LOCUS       pDendra2.        4123 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing Dendra2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pDendra2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Bacterial expression vector.
FEATURES             Location/Qualifiers
     source          1..4123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             114..116
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             132..821
                     /label=Dendra2
                     /note="monomeric photoswitchable green-to-red fluorescent 
                     protein from Dendronephthya"
     terminator      870..964
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1008..1664
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1732..1818
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1973..2113
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2299..2887)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3061..3918)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3919..4023)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttgattaaag aggagaaatt aatatgagag
      121 gatcgggatc catgaacacc ccgggaatta acctgatcaa ggaggacatg cgcgtgaagg
      181 tgcacatgga gggcaacgtg aacggccacg ccttcgtgat cgagggcgag ggcaagggca
      241 agccctacga gggcacccag accgccaacc tgaccgtgaa ggagggcgcc cccctgccct
      301 tcagctacga catcctgacc accgccgtgc actacggcaa ccgggtgttc accaagtacc
      361 ccgaggacat ccccgactac ttcaagcaga gcttccccga gggctacagc tgggagcgca
      421 ccatgacctt cgaggacaag ggcatctgca ccatccgcag cgacatcagc ctggagggcg
      481 actgcttctt ccagaacgtg cgcttcaagg gcaccaactt cccccccaac ggccccgtga
      541 tgcagaagaa gaccctgaag tgggagccca gcaccgagaa gctgcacgtg cgcgacggcc
      601 tgctggtggg caacatcaac atggccctgc tgctggaggg cggcggccac tacctgtgcg
      661 acttcaagac cacctacaag gccaagaagg tggtgcagct gcccgacgcc cacttcgtgg
      721 accaccgcat cgagatcctg ggcaacgaca gcgactacaa caaggtgaag ctgtacgagc
      781 acgccgtggc ccgctacagc cccctgccca gccaggtgtg gtccggcgac agcggcgtgt
      841 acaagtgaaa gcttaattag ctgagcttgg actcctgttg atagatccag taatgacctc
      901 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
      961 gaatccaagc tagcttggcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
     1021 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     1081 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     1141 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     1201 gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat
     1261 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     1321 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     1381 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     1441 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     1501 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     1561 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     1621 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
     1681 gttattggtg cccttaaacg cctggggtaa tgactctcta gcttgaggca tcaaataaaa
     1741 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1801 ctcctgagta ggacaaatcc gccctctaga gctgcctcgc gcgtttcggt gatgacggtg
     1861 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     1921 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     1981 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     2041 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2101 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2161 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2221 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2281 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2341 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2401 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2461 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2521 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2581 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2641 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2701 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2761 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2821 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2881 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2941 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3001 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3061 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3121 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3181 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3241 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3301 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3361 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3421 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3481 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3541 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3601 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3661 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3721 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3781 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3841 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3901 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3961 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4021 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     4081 aacctataaa aataggcgta tcacgaggcc ctttcgtctt cac
//
