LOCUS       62056_8665       11428 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        261..640
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        641..844
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        886..904
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             954..974
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             972..5075
                     /codon_start=1
                     /label=Cas9(H840A)
                     /note="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /translation="VDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7344..7364
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             7419..7439
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             7440..7466
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     polyA_signal    8998..9222
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(9341..9371)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9386..9407)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9695..10283)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10457..11314)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11315..11419)
                     /label=AmpR promoter
ORIGIN
        1 ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc
       61 tactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
      121 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
      181 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
      241 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
      301 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      361 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      421 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      481 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      541 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      601 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      661 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      721 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      781 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      841 agctggttta gtgaaccgtc agatccgcta gagatccgcg gccgctaata cgactcacta
      901 tagggagagc cgccaccatg aaacggacag ccgacggaag cgagttcgag tcaccaaaga
      961 agaagcggaa agtcgacaag aagtacagca tcggcctgga catcggcacc aactctgtgg
     1021 gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca
     1081 acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg
     1141 aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga
     1201 accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct
     1261 tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc
     1321 ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc
     1381 acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg
     1441 ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg
     1501 acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg
     1561 aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga
     1621 gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc
     1681 tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg
     1741 acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca
     1801 acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt
     1861 ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc
     1921 tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag
     1981 ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga
     2041 acggctacgc cggctacatt gacggcggag ccagccagga agagttctac aagttcatca
     2101 agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg
     2161 acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg
     2221 gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc
     2281 gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca
     2341 ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga
     2401 acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca
     2461 acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt
     2521 acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc
     2581 ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc
     2641 ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact
     2701 ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc
     2761 tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg
     2821 aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga
     2881 aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca
     2941 ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca
     3001 agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga
     3061 tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg
     3121 gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca
     3181 tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg
     3241 agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca
     3301 gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga
     3361 aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc
     3421 agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg
     3481 atgtggacgc tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc
     3541 tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga
     3601 agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt
     3661 tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca
     3721 tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact
     3781 cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca
     3841 ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg
     3901 agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc
     3961 tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg
     4021 acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact
     4081 tcttctacag caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga
     4141 tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg
     4201 gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa
     4261 agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg
     4321 ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc
     4381 ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac
     4441 tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga
     4501 atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca
     4561 agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg
     4621 ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt
     4681 acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc
     4741 tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct
     4801 ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc
     4861 accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca
     4921 atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca
     4981 ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg
     5041 agacacggat cgacctgtct cagctgggag gtgactctgg aggatctagc ggaggatcct
     5101 ctggcagcga gacaccagga acaagcgagt cagcaacacc agagagctct ggtagcgaga
     5161 cacccggtac cagtgaaagc gccacgccag aaagcagtgg gagtgagact ccgggtacat
     5221 ctgaatcagc gacaccggaa tcaagtggcg gcagcagtgg cggcagcagc accctaaata
     5281 tagaagatga gtatcggcta catgagacct caaaagagcc agatgtttct ctagggtcca
     5341 catggctgtc tgattttcct caggcctggg cggaaaccgg gggcatggga ctggcagttc
     5401 gccaagctcc tctgatcata cctctgaaag caacctctac ccccgtgtcc ataaaacaat
     5461 accccatgtc acaagaagcc agactgggga tcaagcccca catacagaga ctgttggacc
     5521 agggaatact ggtaccctgc cagtccccct ggaacacgcc cctgctaccc gttaagaaac
     5581 cagggactaa tgattatagg cctgtccagg atctgagaga agtcaacaag cgggtggaag
     5641 acatccaccc caccgtgccc aacccttaca acctcttgag cgggccccca ccgtcccacc
     5701 agtggtacac tgtgcttgat ttaaaggatg cctttttctg cctgagactc caccccacca
     5761 gtcagcctct cttcgccttt gagtggagag atccagagat gggaatctca ggacaattga
     5821 cctggaccag actcccacag ggtttcaaaa acagtcccac cctgtttaat gaggcactgc
     5881 acagagacct agcagacttc cggatccagc acccagactt gatcctgcta cagtacgtgg
     5941 atgacttact gctggccgcc acttctgagc tagactgcca acaaggtact cgggccctgt
     6001 tacaaaccct agggaacctc gggtatcggg cctcggccaa gaaagcccaa atttgccaga
     6061 aacaggtcaa gtatctgggg tatcttctaa aagagggtca gagatggctg actgaggcca
     6121 gaaaagagac tgtgatgggg cagcctactc cgaagacccc tcgacaacta agggagttcc
     6181 tagggaaggc aggcttctgt cgcctcttca tccctgggtt tgcagaaatg gcagcccccc
     6241 tgtaccctct caccaaaccg gggactctgt ttaattgggg cccagaccaa caaaaggcct
     6301 atcaagaaat caagcaagct cttctaactg ccccagccct ggggttgcca gatttgacta
     6361 agccctttga actctttgtc gacgagaagc agggctacgc caaaggtgtc ctaacgcaaa
     6421 aactgggacc ttggcgtcgg ccggtggcct acctgtccaa aaagctagac ccagtagcag
     6481 ctgggtggcc cccttgccta cggatggtag cagccattgc cgtactgaca aaggatgcag
     6541 gcaagctaac catgggacag ccactagtca ttctggcccc ccatgcagta gaggcactag
     6601 tcaaacaacc ccccgaccgc tggctttcca acgcccggat gactcactat caggccttgc
     6661 ttttggacac ggaccgggtc cagttcggac cggtggtagc cctgaacccg gctacgctgc
     6721 tcccactgcc tgaggaaggg ctgcaacaca actgccttga tatcctggcc gaagcccacg
     6781 gaacccgacc cgacctaacg gaccagccgc tcccagacgc cgaccacacc tggtacacgg
     6841 atggaagcag tctcttacaa gagggacagc gtaaggcggg agctgcggtg accaccgaga
     6901 ccgaggtaat ctgggctaaa gccctgccag ccgggacatc cgctcagcgg gctgaactga
     6961 tagcactcac ccaggcccta aagatggcag aaggtaagaa gctaaatgtt tatactgata
     7021 gccgttatgc ttttgctact gcccatatcc atggagaaat atacagaagg cgtgggtggc
     7081 tcacatcaga aggcaaagag atcaaaaata aagacgagat cttggcccta ctaaaagccc
     7141 tctttctgcc caaaagactt agcataatcc attgtccagg acatcaaaag ggacacagcg
     7201 ccgaggctag aggcaaccgg atggctgacc aagcggcccg aaaggcagcc atcacagaga
     7261 ctccagacac ctctaccctc ctcatagaaa attcatcacc ctctggcggc tcaaaaagaa
     7321 ccgccgacgg cagcgaattc gagcccaaga agaagaggaa agtcgggggg tcaggtggat
     7381 ccggcggaag tggcggatcc ggtggatctg gcggcagtcc aaaaaagaaa agaaaagtgt
     7441 atccctatga tgtccccgat tatgccggtt caagagccct ggtcgtgatt agactgagcc
     7501 gagtgacaga cgccaccaca agtcccgaga gacagctgga atcatgccag cagctctgtg
     7561 ctcagcgggg ttgggatgtg gtcggcgtgg cagaggatct ggacgtgagc ggggccgtcg
     7621 atccattcga cagaaagagg aggcccaacc tggcaagatg gctcgctttc gaggaacagc
     7681 cctttgatgt gatcgtcgcc tacagagtgg accggctgac ccgctcaatt cgacatctcc
     7741 agcagctggt gcattgggct gaggaccaca agaaactggt ggtcagcgca acagaagccc
     7801 acttcgatac taccacacct tttgccgctg tggtcatcgc actgatgggc actgtggccc
     7861 agatggagct cgaagctatc aaggagcgaa acaggagcgc agcccatttc aatattaggg
     7921 ccggtaaata cagaggctcc ctgccccctt ggggatatct ccctaccagg gtggatgggg
     7981 agtggagact ggtgccagac cccgtccaga gagagcggat tctggaagtg taccacagag
     8041 tggtcgataa ccacgaacca ctccatctgg tggcacacga cctgaataga cgcggcgtgc
     8101 tctctccaaa ggattatttt gctcagctgc agggaagaga gccacaggga agagaatgga
     8161 gtgctactgc actgaagaga tctatgatca gtgaggctat gctgggttac gcaacactca
     8221 atggcaaaac tgtccgggac gatgacggag cccctctggt gagggctgag cctattctca
     8281 ccagagagca gctcgaagct ctgcgggcag aactggtcaa gactagtcgc gccaaacctg
     8341 ccgtgagcac cccaagcctg ctcctgaggg tgctgttctg cgccgtctgt ggagagccag
     8401 catacaagtt tgccggcgga gggcgcaaac atccccgcta tcgatgcagg agcatggggt
     8461 tccctaagca ctgtggaaac gggacagtgg ccatggctga gtgggacgcc ttttgcgagg
     8521 aacaggtgct ggatctcctg ggtgacgctg agcggctgga aaaagtgtgg gtggcaggat
     8581 ctgactccgc tgtggagctg gcagaagtca atgccgagct cgtggatctg acttccctca
     8641 tcggatctcc tgcatataga gctgggtccc cacagagaga agctctggac gcacgaattg
     8701 ctgcactcgc tgctagacag gaggaactgg agggcctgga ggccaggccc tctggatggg
     8761 agtggcgaga aaccggacag aggtttgggg attggtggag ggagcaggac accgcagcca
     8821 agaacacatg gctgagatcc atgaatgtcc ggctcacatt cgacgtgcgc ggtggcctga
     8881 ctcgaaccat cgattttggc gacctgcagg agtatgaaca gcacctgaga ctggggtccg
     8941 tggtcgaaag actgcacact gggatgtcct aggtttaaac ccgctgatca gcctcgactg
     9001 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     9061 aaggtgccac tcccactgtc ctttcctaat aaaatgagaa aattgcatcg cattgtctga
     9121 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     9181 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     9241 ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     9301 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     9361 taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     9421 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     9481 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     9541 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9601 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9661 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9721 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9781 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9841 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9901 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9961 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    10021 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    10081 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    10141 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    10201 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    10261 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacactc
    10321 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    10381 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    10441 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    10501 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    10561 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    10621 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    10681 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10741 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10801 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10861 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10921 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10981 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    11041 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    11101 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    11161 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    11221 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    11281 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    11341 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    11401 aacaaatagg ggttccgcgc acatttcc
//
