LOCUS       40924_16370        7289 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDV-NTAP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Vectors for expression of proteins with single or combinatorial 
            fluorescent protein and tandem affinity purification tags in 
            Dictyostelium
  JOURNAL   Protein Expr. Purif. 53 (2), 283-288 (2007)
  PUBMED    17296313
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2006) School of Life Sciences, University of 
            Dundee, Dow Street, Dundee DD1 5EH, United Kingdom
REFERENCE   3  (bases 1 to 7289)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7289)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr. Purif."; date: "2007"; volume: "53"; issue: "2"; pages: 
            "283-288"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-SEP-2006) School of Life Sciences, University of Dundee, Dow 
            Street, Dundee DD1 5EH, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             48..221
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     CDS             225..395
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
                     EAKKLNDAQAPK"
     CDS             432..452
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             459..536
                     /label=CBP
                     /note="calmodulin-binding peptide"
     CDS             537..551
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    555..587
                     /label=multipe cloning site
                     /note="multipe cloning site"
     regulatory      599..1579
                     /label=2H3 terminator
                     /note="2H3 terminator"
                     /regulatory_class="terminator"
     CDS             2348..3139
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        4032..4136
                     /label=AmpR promoter
     CDS             4137..4994
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5168..5756
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      6981..7289
                     /label=actin15 promoter
                     /note="actin15 promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 agatctaaaa aatgggatcc gcaggccttg cgcaacacga tgaagccgtg gacaacaaat
       61 tcaacaaaga acaacaaaac gcgttctatg agatcttaca tttacctaac ttaaacgaag
      121 aacaacgaaa cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt
      181 tagcagaagc taaaaagcta aatgatgctc aggcgccgaa agtagacaac aaattcaaca
      241 aagaacaaca aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac
      301 gaaacgcctt catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag
      361 aagctaaaaa gctaaatgat gctcaggcgc cgaaagtaga cgcgaattgt gatataccta
      421 caactgcttc tgaaaattta tattttcagg gtgaactaaa gagaagatgg aaaaagaatt
      481 tcatagccgt ctcagcagcc aaccgcttta agaaaatctc atcctccggg gcacttgatg
      541 atgacgataa gcttactagt tctagagaat tcatcgatta actcgaggat ctgctagagg
      601 taccacaagt ggtggtggtg gtatgggttc atttggtggt cgatccgatg aagaatcaag
      661 tgatccaaat gctatacttg gtctctttga agacgatatt ttctggggtg ataatgatga
      721 atactttagt gataactacc gttatgttga tgaagaagat attgatgaag atttcaatgg
      781 taatgatgaa tttgaaattg atgttgaagg tgatttccaa gaagatgatt tcgaagaaga
      841 atatgcattt tattaaatta aacttttttt cataaaaaaa taataataat aatttaataa
      901 ctaatcatta tcatcaataa taacattata atattacttt aataacaata ctttttttta
      961 atattcaaag actttacaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaca aaccataaac
     1021 aaaactaaaa ataaattaaa aaaaaaaata aaaaaaaaaa aaaaaaaaaa aactaaaaat
     1081 acaattattt tattatttaa ataaataatt gtattataat ttgtattttt gtgtttttgt
     1141 tttttttatt tctttaaaat gatttataac ttttattttt tataaaggaa aataacattt
     1201 tccttaaatg ataaaagatc atttaaaaca tttgtattaa aaggttcatt accattatat
     1261 aaatcattaa ataattgttt ttgaactgtg aaaacgaatt tagtatgttt caaatcagtg
     1321 atttgtggat tatcaactaa tattgattga attgttggtg tatcatcatg agcataagaa
     1381 ccatcatcca ttatactttc aacatgaaaa tattttgtaa tttttttatt gattgatgaa
     1441 atatatctaa tgagttgatc agaatttaaa cctaatattg ataattttct taacggtgca
     1501 attgatgaaa tcattgaacc aaactcttta acgaatgcat tggataactt tatcgatctt
     1561 tgttttcttt gagagaatta gcggccgcta attcagaggt ttatttttaa aaattacaaa
     1621 aactaaaaag aaaaataaaa aggaaaaatc ttattaatct gaaaattaca gatttgcccc
     1681 aatttaaaga aaaattatcc aaaataatca atcaacaacg atatcttttt gatagatatt
     1741 tataaaaact ttatcttttt tattttttca aagttgcgca aaataataaa attaaaataa
     1801 atataaaaac tgtaaaaaga aaaaaaaaag tgtaaaggtt tattaattat ttaattatta
     1861 ttcacttttt gtaattattt ttttattttg aagaataatg atggatattt tatataaaaa
     1921 aaaaaagaga tatgaaaaaa taataattat aaaaaaaaaa aaaaaaaata gaattttaaa
     1981 gttttagtac aaatggatga tttttttttt tttttttttt ttttcctaaa taattcaagt
     2041 aataaacaaa gaattgatat tctgatgcct aattaaaaaa gaaattttta aataaaaaat
     2101 gggttttttt taagtaaagt tatttgaaat tgattgaaat tttcaaacca tgggtggttt
     2161 ttcgctttaa aattgggatt ttatttttat ttttttatat tttttatttt ttattttttt
     2221 tttttttgag gtttctgaga ttataaaatg aaattttttt ttttttttta attaattcaa
     2281 aaaataatca aataaataaa tataatataa aatggacgga gaagatgttc aagctggatc
     2341 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     2401 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     2461 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     2521 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     2581 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     2641 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     2701 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     2761 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     2821 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     2881 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     2941 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     3001 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     3061 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     3121 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     3181 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     3241 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     3301 ttcttcgccc accccgggct cgatcccctc gcgagttggt tcagctgctg cctgaggctg
     3361 gacgacctcg cggagttcta ccggcagtgc aaatccgtcg gcatccagga aaccagcagc
     3421 ggctatccgc gcatccatgc ccccgaactg caggagtggg gaggcacgat ggccgctttg
     3481 gtcccggatc agctttatct ttttgatttt catactcttt ggctgtttta catctaatgc
     3541 atatatttat cttcaccatt caaaatgtat ctgtaaataa taaatttttt aaatatattt
     3601 gatttttttg atagaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaat taaaataact
     3661 taataattat taagatttaa aaaaaaaaaa ttaaaaaaaa aagaaaatat tagattatta
     3721 caacaatttg tttttttatt atttattaaa taatttagaa acattttctg tgaacaattg
     3781 atggaccctc aggaagatcg cactccagcc agctttccgg caccgcttct ggtgccggaa
     3841 accaggcaaa gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgg
     3901 cggggatcag cggccgctat cagatcaatt cttgaagacg aaagggcctc gtgatacgcc
     3961 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     4021 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4081 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4141 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4201 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4261 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4321 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     4381 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4441 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4501 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4561 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4621 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     4681 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     4741 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     4801 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     4861 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     4921 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     4981 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5041 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5101 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5161 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5221 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5281 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     5341 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5401 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5461 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5521 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5581 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5641 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5701 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5761 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5821 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5881 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5941 gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt
     6001 gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat
     6061 tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc
     6121 acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg
     6181 ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt
     6241 ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc
     6301 cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc
     6361 agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg
     6421 cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg
     6481 cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct
     6541 gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc
     6601 agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga
     6661 taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga
     6721 gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa
     6781 agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga
     6841 taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc
     6901 tgactgggtt gaaggctctc aagggcatcg gtcgagcagc tgaagctagc ttgcatgcct
     6961 gcaggtcgac tctagctaga taaaaaaaat ttttatttat ttttatttat tttgaattaa
     7021 atagattaca aattaattaa tcccatcaaa tctttaaaaa aaaaaatggt ttaaaaaaac
     7081 ttgggttggt taattattat ttgaaaattt taaaacccaa attaaaaaaa aaaaatggga
     7141 ttcaaaaatt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
     7201 ttttttcaga ttgcataaaa agattttttt tttttttttt ttcttatttc ttaaaacaaa
     7261 taaattaaat taaataaaaa ataaaaatc
//
