LOCUS       40924_16355        8003 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDV-NTAP-CGFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Vectors for expression of proteins with single or combinatorial 
            fluorescent protein and tandem affinity purification tags in 
            Dictyostelium
  JOURNAL   Protein Expr. Purif. 53 (2), 283-288 (2007)
  PUBMED    17296313
REFERENCE   2  (bases 1 to 8003)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2006) School of Life Sciences, University of 
            Dundee, Dow Street, Dundee DD1 5EH, United Kingdom
REFERENCE   3  (bases 1 to 8003)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8003)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr. Purif."; date: "2007"; volume: "53"; issue: "2"; pages: 
            "283-288"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-SEP-2006) School of Life Sciences, University of Dundee, Dow 
            Street, Dundee DD1 5EH, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             48..221
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             225..395
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
                     EAKKLNDAQAPK"
     CDS             432..452
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             459..536
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             537..551
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    555..572
                     /label=multipe cloning site
                     /note="multipe cloning site"
     CDS             573..1286
                     /label=EGFP
                     /note="enhanced GFP"
     regulatory      1313..2293
                     /label=2H3 terminator
                     /note="2H3 terminator"
                     /regulatory_class="terminator"
     CDS             3062..3853
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        4746..4850
                     /label=AmpR promoter
     CDS             4851..5708
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5882..6470
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      7695..8003
                     /label=actin15 promoter
                     /note="actin15 promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 agatctaaaa aatgggatcc gcaggccttg cgcaacacga tgaagccgtg gacaacaaat
       61 tcaacaaaga acaacaaaac gcgttctatg agatcttaca tttacctaac ttaaacgaag
      121 aacaacgaaa cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt
      181 tagcagaagc taaaaagcta aatgatgctc aggcgccgaa agtagacaac aaattcaaca
      241 aagaacaaca aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac
      301 gaaacgcctt catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag
      361 aagctaaaaa gctaaatgat gctcaggcgc cgaaagtaga cgcgaattgt gatataccta
      421 caactgcttc tgaaaattta tattttcagg gtgaactaaa gagaagatgg aaaaagaatt
      481 tcatagccgt ctcagcagcc aaccgcttta agaaaatctc atcctccggg gcacttgatg
      541 atgacgataa gcttactagt tctagagaat tcgtgagcaa gggcgaggag ctgttcaccg
      601 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      661 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      721 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
      781 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      841 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      901 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      961 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1021 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1081 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1141 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     1201 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1261 ctctcggcat ggacgagctg tacaagatcg attaactcga ggatctgcta gaggtaccac
     1321 aagtggtggt ggtggtatgg gttcatttgg tggtcgatcc gatgaagaat caagtgatcc
     1381 aaatgctata cttggtctct ttgaagacga tattttctgg ggtgataatg atgaatactt
     1441 tagtgataac taccgttatg ttgatgaaga agatattgat gaagatttca atggtaatga
     1501 tgaatttgaa attgatgttg aaggtgattt ccaagaagat gatttcgaag aagaatatgc
     1561 attttattaa attaaacttt ttttcataaa aaaataataa taataattta ataactaatc
     1621 attatcatca ataataacat tataatatta ctttaataac aatacttttt tttaatattc
     1681 aaagacttta caaaaaaaaa aaaaaaaaaa aaaaaaaaaa aacaaaccat aaacaaaact
     1741 aaaaataaat taaaaaaaaa aataaaaaaa aaaaaaaaaa aaaaaactaa aaatacaatt
     1801 attttattat ttaaataaat aattgtatta taatttgtat ttttgtgttt ttgttttttt
     1861 tatttcttta aaatgattta taacttttat tttttataaa ggaaaataac attttcctta
     1921 aatgataaaa gatcatttaa aacatttgta ttaaaaggtt cattaccatt atataaatca
     1981 ttaaataatt gtttttgaac tgtgaaaacg aatttagtat gtttcaaatc agtgatttgt
     2041 ggattatcaa ctaatattga ttgaattgtt ggtgtatcat catgagcata agaaccatca
     2101 tccattatac tttcaacatg aaaatatttt gtaatttttt tattgattga tgaaatatat
     2161 ctaatgagtt gatcagaatt taaacctaat attgataatt ttcttaacgg tgcaattgat
     2221 gaaatcattg aaccaaactc tttaacgaat gcattggata actttatcga tctttgtttt
     2281 ctttgagaga attagcggcc gctaattcag aggtttattt ttaaaaatta caaaaactaa
     2341 aaagaaaaat aaaaaggaaa aatcttatta atctgaaaat tacagatttg ccccaattta
     2401 aagaaaaatt atccaaaata atcaatcaac aacgatatct ttttgataga tatttataaa
     2461 aactttatct tttttatttt ttcaaagttg cgcaaaataa taaaattaaa ataaatataa
     2521 aaactgtaaa aagaaaaaaa aaagtgtaaa ggtttattaa ttatttaatt attattcact
     2581 ttttgtaatt atttttttat tttgaagaat aatgatggat attttatata aaaaaaaaaa
     2641 gagatatgaa aaaataataa ttataaaaaa aaaaaaaaaa aatagaattt taaagtttta
     2701 gtacaaatgg atgatttttt tttttttttt ttttttttcc taaataattc aagtaataaa
     2761 caaagaattg atattctgat gcctaattaa aaaagaaatt tttaaataaa aaatgggttt
     2821 tttttaagta aagttatttg aaattgattg aaattttcaa accatgggtg gtttttcgct
     2881 ttaaaattgg gattttattt ttattttttt atatttttta ttttttattt tttttttttt
     2941 tgaggtttct gagattataa aatgaaattt tttttttttt tttaattaat tcaaaaaata
     3001 atcaaataaa taaatataat ataaaatgga cggagaagat gttcaagctg gatcgtttcg
     3061 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     3121 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     3181 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     3241 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     3301 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     3361 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     3421 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     3481 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     3541 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     3601 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     3661 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     3721 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     3781 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     3841 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     3901 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     3961 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     4021 gcccaccccg ggctcgatcc cctcgcgagt tggttcagct gctgcctgag gctggacgac
     4081 ctcgcggagt tctaccggca gtgcaaatcc gtcggcatcc aggaaaccag cagcggctat
     4141 ccgcgcatcc atgcccccga actgcaggag tggggaggca cgatggccgc tttggtcccg
     4201 gatcagcttt atctttttga ttttcatact ctttggctgt tttacatcta atgcatatat
     4261 ttatcttcac cattcaaaat gtatctgtaa ataataaatt ttttaaatat atttgatttt
     4321 tttgatagaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaattaaaat aacttaataa
     4381 ttattaagat ttaaaaaaaa aaaattaaaa aaaaaagaaa atattagatt attacaacaa
     4441 tttgtttttt tattatttat taaataattt agaaacattt tctgtgaaca attgatggac
     4501 cctcaggaag atcgcactcc agccagcttt ccggcaccgc ttctggtgcc ggaaaccagg
     4561 caaagcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtggcgggga
     4621 tcagcggccg ctatcagatc aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     4681 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     4741 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4801 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4861 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4921 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4981 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5041 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     5101 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5161 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5221 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5281 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5341 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     5401 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5461 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5521 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5581 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5641 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5701 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5761 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5821 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5881 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5941 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6001 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     6061 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6121 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6181 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6241 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6301 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6361 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6421 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6481 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6541 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6601 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgctgac
     6661 ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag
     6721 gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc
     6781 tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc
     6841 atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag
     6901 atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc
     6961 aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt
     7021 ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa
     7081 ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat
     7141 aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg
     7201 atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg
     7261 cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc
     7321 gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg
     7381 cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt
     7441 gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt
     7501 cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga
     7561 agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg
     7621 ggttgaaggc tctcaagggc atcggtcgag cagctgaagc tagcttgcat gcctgcaggt
     7681 cgactctagc tagataaaaa aaatttttat ttatttttat ttattttgaa ttaaatagat
     7741 tacaaattaa ttaatcccat caaatcttta aaaaaaaaaa tggtttaaaa aaacttgggt
     7801 tggttaatta ttatttgaaa attttaaaac ccaaattaaa aaaaaaaaat gggattcaaa
     7861 aatttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
     7921 cagattgcat aaaaagattt tttttttttt ttttttctta tttcttaaaa caaataaatt
     7981 aaattaaata aaaaataaaa atc
//
