LOCUS       40924_16350        7475 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDV-CYFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Vectors for expression of proteins with single or combinatorial 
            fluorescent protein and tandem affinity purification tags in 
            Dictyostelium
  JOURNAL   Protein Expr. Purif. 53 (2), 283-288 (2007)
  PUBMED    17296313
REFERENCE   2  (bases 1 to 7475)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2006) School of Life Sciences, University of 
            Dundee, Dow Street, Dundee DD1 5EH, United Kingdom
REFERENCE   3  (bases 1 to 7475)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7475)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr. Purif."; date: "2007"; volume: "53"; issue: "2"; pages: 
            "283-288"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-SEP-2006) School of Life Sciences, University of Dundee, Dow 
            Street, Dundee DD1 5EH, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    15..44
                     /label=multipe cloning site
                     /note="multipe cloning site"
     CDS             45..758
                     /label=EYFP
                     /note="enhanced YFP"
     regulatory      785..1765
                     /label=2H3 terminator
                     /note="2H3 terminator"
                     /regulatory_class="terminator"
     CDS             2534..3325
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        4218..4322
                     /label=AmpR promoter
     CDS             4323..5180
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5354..5942
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      7167..7475
                     /label=actin15 promoter
                     /note="actin15 promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 agatctaaaa aatgggatcc aagcttacta gttctagaga attcgtgagc aagggcgagg
       61 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      121 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      181 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
      241 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      301 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      361 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      421 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
      481 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
      541 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      601 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg
      661 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      721 ccgccgggat cactctcggc atggacgagc tgtacaagat cgattaactc gaggatctgc
      781 tagaggtacc acaagtggtg gtggtggtat gggttcattt ggtggtcgat ccgatgaaga
      841 atcaagtgat ccaaatgcta tacttggtct ctttgaagac gatattttct ggggtgataa
      901 tgatgaatac tttagtgata actaccgtta tgttgatgaa gaagatattg atgaagattt
      961 caatggtaat gatgaatttg aaattgatgt tgaaggtgat ttccaagaag atgatttcga
     1021 agaagaatat gcattttatt aaattaaact ttttttcata aaaaaataat aataataatt
     1081 taataactaa tcattatcat caataataac attataatat tactttaata acaatacttt
     1141 tttttaatat tcaaagactt tacaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaacaaacc
     1201 ataaacaaaa ctaaaaataa attaaaaaaa aaaataaaaa aaaaaaaaaa aaaaaaaact
     1261 aaaaatacaa ttattttatt atttaaataa ataattgtat tataatttgt atttttgtgt
     1321 ttttgttttt tttatttctt taaaatgatt tataactttt attttttata aaggaaaata
     1381 acattttcct taaatgataa aagatcattt aaaacatttg tattaaaagg ttcattacca
     1441 ttatataaat cattaaataa ttgtttttga actgtgaaaa cgaatttagt atgtttcaaa
     1501 tcagtgattt gtggattatc aactaatatt gattgaattg ttggtgtatc atcatgagca
     1561 taagaaccat catccattat actttcaaca tgaaaatatt ttgtaatttt tttattgatt
     1621 gatgaaatat atctaatgag ttgatcagaa tttaaaccta atattgataa ttttcttaac
     1681 ggtgcaattg atgaaatcat tgaaccaaac tctttaacga atgcattgga taactttatc
     1741 gatctttgtt ttctttgaga gaattagcgg ccgctaattc agaggtttat ttttaaaaat
     1801 tacaaaaact aaaaagaaaa ataaaaagga aaaatcttat taatctgaaa attacagatt
     1861 tgccccaatt taaagaaaaa ttatccaaaa taatcaatca acaacgatat ctttttgata
     1921 gatatttata aaaactttat cttttttatt ttttcaaagt tgcgcaaaat aataaaatta
     1981 aaataaatat aaaaactgta aaaagaaaaa aaaaagtgta aaggtttatt aattatttaa
     2041 ttattattca ctttttgtaa ttattttttt attttgaaga ataatgatgg atattttata
     2101 taaaaaaaaa aagagatatg aaaaaataat aattataaaa aaaaaaaaaa aaaatagaat
     2161 tttaaagttt tagtacaaat ggatgatttt tttttttttt tttttttttt cctaaataat
     2221 tcaagtaata aacaaagaat tgatattctg atgcctaatt aaaaaagaaa tttttaaata
     2281 aaaaatgggt tttttttaag taaagttatt tgaaattgat tgaaattttc aaaccatggg
     2341 tggtttttcg ctttaaaatt gggattttat ttttattttt ttatattttt tattttttat
     2401 tttttttttt tttgaggttt ctgagattat aaaatgaaat tttttttttt tttttaatta
     2461 attcaaaaaa taatcaaata aataaatata atataaaatg gacggagaag atgttcaagc
     2521 tggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     2581 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     2641 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     2701 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     2761 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     2821 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     2881 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     2941 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     3001 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     3061 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     3121 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     3181 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     3241 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     3301 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     3361 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     3421 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     3481 ctggagttct tcgcccaccc cgggctcgat cccctcgcga gttggttcag ctgctgcctg
     3541 aggctggacg acctcgcgga gttctaccgg cagtgcaaat ccgtcggcat ccaggaaacc
     3601 agcagcggct atccgcgcat ccatgccccc gaactgcagg agtggggagg cacgatggcc
     3661 gctttggtcc cggatcagct ttatcttttt gattttcata ctctttggct gttttacatc
     3721 taatgcatat atttatcttc accattcaaa atgtatctgt aaataataaa ttttttaaat
     3781 atatttgatt tttttgatag aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaattaaa
     3841 ataacttaat aattattaag atttaaaaaa aaaaaattaa aaaaaaaaga aaatattaga
     3901 ttattacaac aatttgtttt tttattattt attaaataat ttagaaacat tttctgtgaa
     3961 caattgatgg accctcagga agatcgcact ccagccagct ttccggcacc gcttctggtg
     4021 ccggaaacca ggcaaagcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     4081 cggtggcggg gatcagcggc cgctatcaga tcaattcttg aagacgaaag ggcctcgtga
     4141 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     4201 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4261 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4321 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4381 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4441 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4501 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4561 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4621 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4681 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4741 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4801 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4861 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4921 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4981 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5041 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5101 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5161 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5221 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5281 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5341 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5401 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5461 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     5521 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5581 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5641 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5701 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5761 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5821 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5881 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5941 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6001 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6061 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6121 aagagcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat
     6181 gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc
     6241 ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac
     6301 aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg
     6361 cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc
     6421 acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg
     6481 tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg
     6541 ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg
     6601 ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa
     6661 gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca
     6721 tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg
     6781 ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc
     6841 cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt
     6901 gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc
     6961 agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt
     7021 gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga
     7081 aggagctgac tgggttgaag gctctcaagg gcatcggtcg agcagctgaa gctagcttgc
     7141 atgcctgcag gtcgactcta gctagataaa aaaaattttt atttattttt atttattttg
     7201 aattaaatag attacaaatt aattaatccc atcaaatctt taaaaaaaaa aatggtttaa
     7261 aaaaacttgg gttggttaat tattatttga aaattttaaa acccaaatta aaaaaaaaaa
     7321 atgggattca aaaatttttt tttttttttt tttttttttt tttttttttt tttttttttt
     7381 tttttttttt ttcagattgc ataaaaagat tttttttttt ttttttttct tatttcttaa
     7441 aacaaataaa ttaaattaaa taaaaaataa aaatc
//
