LOCUS       40924_16345        8021 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDV-CYFP-CTAP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Vectors for expression of proteins with single or combinatorial 
            fluorescent protein and tandem affinity purification tags in 
            Dictyostelium
  JOURNAL   Protein Expr. Purif. 53 (2), 283-288 (2007)
  PUBMED    17296313
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2006) School of Life Sciences, University of 
            Dundee, Dow Street, Dundee DD1 5EH, United Kingdom
REFERENCE   3  (bases 1 to 8021)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8021)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr. Purif."; date: "2007"; volume: "53"; issue: "2"; pages: 
            "283-288"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-SEP-2006) School of Life Sciences, University of Dundee, Dow 
            Street, Dundee DD1 5EH, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    15..44
                     /label=multipe cloning site
                     /note="multipe cloning site"
     CDS             45..758
                     /label=EYFP
                     /note="enhanced YFP"
     CDS             768..845
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             873..893
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             933..1106
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1110..1280
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
                     EAKKLNGAQAPK"
     regulatory      1331..2311
                     /label=2H3 terminator
                     /note="2H3 terminator"
                     /regulatory_class="terminator"
     CDS             3080..3871
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        4764..4868
                     /label=AmpR promoter
     CDS             4869..5726
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5900..6488
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      7713..8021
                     /label=actin15 promoter
                     /note="actin15 promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 agatctaaaa aatgggatcc aagcttacta gttctagaga attcgtgagc aagggcgagg
       61 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      121 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      181 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
      241 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      301 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      361 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      421 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
      481 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
      541 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      601 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg
      661 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      721 ccgccgggat cactctcggc atggacgagc tgtacaagat cgatgaaaag agaagatgga
      781 aaaagaattt catagccgtc tcagcagcca accgctttaa gaaaatctca tcctccgggg
      841 cacttgatta tgatattcca actactgcta gcgagaattt gtattttcag ggtgagctca
      901 aaaccgcggc tcttgcgcaa cacgatgaag ccgtggacaa caaattcaac aaagaacaac
      961 aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa cgaagaacaa cgaaacgcct
     1021 tcatccaaag tttaaaagat gacccaagcc aaagcgctaa ccttttagca gaagctaaaa
     1081 agctaaatga tgctcaggcg ccgaaagtag acaacaaatt caacaaagaa caacaaaacg
     1141 cgttctatga gatcttacat ttacctaact taaacgaaga acaacgaaac gccttcatcc
     1201 aaagtttaaa agatgaccca agccaaagcg ctaacctttt agcagaagct aaaaagctaa
     1261 atggtgctca ggcgccgaaa gtagacgcga attccgcggg gaagtcaacc taactcgagg
     1321 atctgctaga ggtaccacaa gtggtggtgg tggtatgggt tcatttggtg gtcgatccga
     1381 tgaagaatca agtgatccaa atgctatact tggtctcttt gaagacgata ttttctgggg
     1441 tgataatgat gaatacttta gtgataacta ccgttatgtt gatgaagaag atattgatga
     1501 agatttcaat ggtaatgatg aatttgaaat tgatgttgaa ggtgatttcc aagaagatga
     1561 tttcgaagaa gaatatgcat tttattaaat taaacttttt ttcataaaaa aataataata
     1621 ataatttaat aactaatcat tatcatcaat aataacatta taatattact ttaataacaa
     1681 tacttttttt taatattcaa agactttaca aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1741 caaaccataa acaaaactaa aaataaatta aaaaaaaaaa taaaaaaaaa aaaaaaaaaa
     1801 aaaactaaaa atacaattat tttattattt aaataaataa ttgtattata atttgtattt
     1861 ttgtgttttt gtttttttta tttctttaaa atgatttata acttttattt tttataaagg
     1921 aaaataacat tttccttaaa tgataaaaga tcatttaaaa catttgtatt aaaaggttca
     1981 ttaccattat ataaatcatt aaataattgt ttttgaactg tgaaaacgaa tttagtatgt
     2041 ttcaaatcag tgatttgtgg attatcaact aatattgatt gaattgttgg tgtatcatca
     2101 tgagcataag aaccatcatc cattatactt tcaacatgaa aatattttgt aattttttta
     2161 ttgattgatg aaatatatct aatgagttga tcagaattta aacctaatat tgataatttt
     2221 cttaacggtg caattgatga aatcattgaa ccaaactctt taacgaatgc attggataac
     2281 tttatcgatc tttgttttct ttgagagaat tagcggccgc taattcagag gtttattttt
     2341 aaaaattaca aaaactaaaa agaaaaataa aaaggaaaaa tcttattaat ctgaaaatta
     2401 cagatttgcc ccaatttaaa gaaaaattat ccaaaataat caatcaacaa cgatatcttt
     2461 ttgatagata tttataaaaa ctttatcttt tttatttttt caaagttgcg caaaataata
     2521 aaattaaaat aaatataaaa actgtaaaaa gaaaaaaaaa agtgtaaagg tttattaatt
     2581 atttaattat tattcacttt ttgtaattat ttttttattt tgaagaataa tgatggatat
     2641 tttatataaa aaaaaaaaga gatatgaaaa aataataatt ataaaaaaaa aaaaaaaaaa
     2701 tagaatttta aagttttagt acaaatggat gatttttttt tttttttttt ttttttccta
     2761 aataattcaa gtaataaaca aagaattgat attctgatgc ctaattaaaa aagaaatttt
     2821 taaataaaaa atgggttttt tttaagtaaa gttatttgaa attgattgaa attttcaaac
     2881 catgggtggt ttttcgcttt aaaattggga ttttattttt atttttttat attttttatt
     2941 ttttattttt tttttttttg aggtttctga gattataaaa tgaaattttt tttttttttt
     3001 taattaattc aaaaaataat caaataaata aatataatat aaaatggacg gagaagatgt
     3061 tcaagctgga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     3121 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     3181 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     3241 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     3301 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     3361 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     3421 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     3481 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     3541 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     3601 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     3661 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     3721 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     3781 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     3841 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     3901 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     3961 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     4021 ctcatgctgg agttcttcgc ccaccccggg ctcgatcccc tcgcgagttg gttcagctgc
     4081 tgcctgaggc tggacgacct cgcggagttc taccggcagt gcaaatccgt cggcatccag
     4141 gaaaccagca gcggctatcc gcgcatccat gcccccgaac tgcaggagtg gggaggcacg
     4201 atggccgctt tggtcccgga tcagctttat ctttttgatt ttcatactct ttggctgttt
     4261 tacatctaat gcatatattt atcttcacca ttcaaaatgt atctgtaaat aataaatttt
     4321 ttaaatatat ttgatttttt tgatagaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4381 attaaaataa cttaataatt attaagattt aaaaaaaaaa aattaaaaaa aaaagaaaat
     4441 attagattat tacaacaatt tgttttttta ttatttatta aataatttag aaacattttc
     4501 tgtgaacaat tgatggaccc tcaggaagat cgcactccag ccagctttcc ggcaccgctt
     4561 ctggtgccgg aaaccaggca aagcgccatt cgccattcag gctgcgcaac tgttgggaag
     4621 ggcgatcggt ggcggggatc agcggccgct atcagatcaa ttcttgaaga cgaaagggcc
     4681 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     4741 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4801 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4861 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4921 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4981 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5041 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5101 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5161 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5221 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5281 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5341 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5401 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5461 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5521 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5581 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5641 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5701 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5761 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5821 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5881 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5941 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6001 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     6061 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6121 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6181 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6241 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6301 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6361 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6421 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6481 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6541 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6601 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6661 aagcggaaga gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc
     6721 attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg
     6781 cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc
     6841 aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc
     6901 cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc
     6961 gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta
     7021 gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca
     7081 acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg
     7141 tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc
     7201 tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg
     7261 acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg
     7321 ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg
     7381 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     7441 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     7501 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     7561 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     7621 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagca gctgaagcta
     7681 gcttgcatgc ctgcaggtcg actctagcta gataaaaaaa atttttattt atttttattt
     7741 attttgaatt aaatagatta caaattaatt aatcccatca aatctttaaa aaaaaaaatg
     7801 gtttaaaaaa acttgggttg gttaattatt atttgaaaat tttaaaaccc aaattaaaaa
     7861 aaaaaaatgg gattcaaaaa tttttttttt tttttttttt tttttttttt tttttttttt
     7921 tttttttttt ttttttttca gattgcataa aaagattttt tttttttttt ttttcttatt
     7981 tcttaaaaca aataaattaa attaaataaa aaataaaaat c
//
