LOCUS       40924_16340        7307 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDV-CTAP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Vectors for expression of proteins with single or combinatorial 
            fluorescent protein and tandem affinity purification tags in 
            Dictyostelium
  JOURNAL   Protein Expr. Purif. 53 (2), 283-288 (2007)
  PUBMED    17296313
REFERENCE   2  (bases 1 to 7307)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2006) School of Life Sciences, University of 
            Dundee, Dow Street, Dundee DD1 5EH, United Kingdom
REFERENCE   3  (bases 1 to 7307)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7307)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr. Purif."; date: "2007"; volume: "53"; issue: "2"; pages: 
            "283-288"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-SEP-2006) School of Life Sciences, University of Dundee, Dow 
            Street, Dundee DD1 5EH, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    15..50
                     /label=multipe cloning site
                     /note="multipe cloning site"
     CDS             54..131
                     /label=CBP
                     /note="calmodulin-binding peptide"
     CDS             159..179
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             219..392
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     CDS             396..566
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
                     EAKKLNGAQAPK"
     regulatory      617..1597
                     /label=2H3 terminator
                     /note="2H3 terminator"
                     /regulatory_class="terminator"
     CDS             2366..3157
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        4050..4154
                     /label=AmpR promoter
     CDS             4155..5012
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5186..5774
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      6999..7307
                     /label=actin15 promoter
                     /note="actin15 promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 agatctaaaa aatgggatcc aagcttacta gttctagaga attcatcgat gaaaagagaa
       61 gatggaaaaa gaatttcata gccgtctcag cagccaaccg ctttaagaaa atctcatcct
      121 ccggggcact tgattatgat attccaacta ctgctagcga gaatttgtat tttcagggtg
      181 agctcaaaac cgcggctctt gcgcaacacg atgaagccgt ggacaacaaa ttcaacaaag
      241 aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa gaacaacgaa
      301 acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt ttagcagaag
      361 ctaaaaagct aaatgatgct caggcgccga aagtagacaa caaattcaac aaagaacaac
      421 aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa cgaagaacaa cgaaacgcct
      481 tcatccaaag tttaaaagat gacccaagcc aaagcgctaa ccttttagca gaagctaaaa
      541 agctaaatgg tgctcaggcg ccgaaagtag acgcgaattc cgcggggaag tcaacctaac
      601 tcgaggatct gctagaggta ccacaagtgg tggtggtggt atgggttcat ttggtggtcg
      661 atccgatgaa gaatcaagtg atccaaatgc tatacttggt ctctttgaag acgatatttt
      721 ctggggtgat aatgatgaat actttagtga taactaccgt tatgttgatg aagaagatat
      781 tgatgaagat ttcaatggta atgatgaatt tgaaattgat gttgaaggtg atttccaaga
      841 agatgatttc gaagaagaat atgcatttta ttaaattaaa ctttttttca taaaaaaata
      901 ataataataa tttaataact aatcattatc atcaataata acattataat attactttaa
      961 taacaatact tttttttaat attcaaagac tttacaaaaa aaaaaaaaaa aaaaaaaaaa
     1021 aaaaaacaaa ccataaacaa aactaaaaat aaattaaaaa aaaaaataaa aaaaaaaaaa
     1081 aaaaaaaaaa ctaaaaatac aattatttta ttatttaaat aaataattgt attataattt
     1141 gtatttttgt gtttttgttt tttttatttc tttaaaatga tttataactt ttatttttta
     1201 taaaggaaaa taacattttc cttaaatgat aaaagatcat ttaaaacatt tgtattaaaa
     1261 ggttcattac cattatataa atcattaaat aattgttttt gaactgtgaa aacgaattta
     1321 gtatgtttca aatcagtgat ttgtggatta tcaactaata ttgattgaat tgttggtgta
     1381 tcatcatgag cataagaacc atcatccatt atactttcaa catgaaaata ttttgtaatt
     1441 tttttattga ttgatgaaat atatctaatg agttgatcag aatttaaacc taatattgat
     1501 aattttctta acggtgcaat tgatgaaatc attgaaccaa actctttaac gaatgcattg
     1561 gataacttta tcgatctttg ttttctttga gagaattagc ggccgctaat tcagaggttt
     1621 atttttaaaa attacaaaaa ctaaaaagaa aaataaaaag gaaaaatctt attaatctga
     1681 aaattacaga tttgccccaa tttaaagaaa aattatccaa aataatcaat caacaacgat
     1741 atctttttga tagatattta taaaaacttt atctttttta ttttttcaaa gttgcgcaaa
     1801 ataataaaat taaaataaat ataaaaactg taaaaagaaa aaaaaaagtg taaaggttta
     1861 ttaattattt aattattatt cactttttgt aattattttt ttattttgaa gaataatgat
     1921 ggatatttta tataaaaaaa aaaagagata tgaaaaaata ataattataa aaaaaaaaaa
     1981 aaaaaataga attttaaagt tttagtacaa atggatgatt tttttttttt tttttttttt
     2041 ttcctaaata attcaagtaa taaacaaaga attgatattc tgatgcctaa ttaaaaaaga
     2101 aatttttaaa taaaaaatgg gtttttttta agtaaagtta tttgaaattg attgaaattt
     2161 tcaaaccatg ggtggttttt cgctttaaaa ttgggatttt atttttattt ttttatattt
     2221 tttatttttt attttttttt tttttgaggt ttctgagatt ataaaatgaa attttttttt
     2281 tttttttaat taattcaaaa aataatcaaa taaataaata taatataaaa tggacggaga
     2341 agatgttcaa gctggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     2401 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     2461 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     2521 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     2581 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     2641 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     2701 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     2761 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     2821 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     2881 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     2941 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     3001 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     3061 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     3121 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     3181 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     3241 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     3301 ggggatctca tgctggagtt cttcgcccac cccgggctcg atcccctcgc gagttggttc
     3361 agctgctgcc tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa atccgtcggc
     3421 atccaggaaa ccagcagcgg ctatccgcgc atccatgccc ccgaactgca ggagtgggga
     3481 ggcacgatgg ccgctttggt cccggatcag ctttatcttt ttgattttca tactctttgg
     3541 ctgttttaca tctaatgcat atatttatct tcaccattca aaatgtatct gtaaataata
     3601 aattttttaa atatatttga tttttttgat agaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3661 aaaaaaatta aaataactta ataattatta agatttaaaa aaaaaaaatt aaaaaaaaaa
     3721 gaaaatatta gattattaca acaatttgtt tttttattat ttattaaata atttagaaac
     3781 attttctgtg aacaattgat ggaccctcag gaagatcgca ctccagccag ctttccggca
     3841 ccgcttctgg tgccggaaac caggcaaagc gccattcgcc attcaggctg cgcaactgtt
     3901 gggaagggcg atcggtggcg gggatcagcg gccgctatca gatcaattct tgaagacgaa
     3961 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     4021 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4081 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4141 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4201 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4261 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4321 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4381 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4441 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4501 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4561 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4621 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4681 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     4741 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4801 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4861 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4921 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4981 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5041 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5101 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5161 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5221 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5281 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     5341 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5401 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5461 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5521 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5581 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5641 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5701 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5761 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5821 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     5881 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     5941 gcgaggaagc ggaagagcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
     6001 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
     6061 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
     6121 gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag
     6181 atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg
     6241 gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat
     6301 ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc
     6361 gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt
     6421 tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag
     6481 ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct
     6541 gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca
     6601 taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt
     6661 cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
     6721 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca
     6781 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
     6841 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc
     6901 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagcagctg
     6961 aagctagctt gcatgcctgc aggtcgactc tagctagata aaaaaaattt ttatttattt
     7021 ttatttattt tgaattaaat agattacaaa ttaattaatc ccatcaaatc tttaaaaaaa
     7081 aaaatggttt aaaaaaactt gggttggtta attattattt gaaaatttta aaacccaaat
     7141 taaaaaaaaa aaatgggatt caaaaatttt tttttttttt tttttttttt tttttttttt
     7201 tttttttttt tttttttttt ttttcagatt gcataaaaag attttttttt tttttttttt
     7261 cttatttctt aaaacaaata aattaaatta aataaaaaat aaaaatc
//
