LOCUS       40924_15820        6639 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pDUAL-GC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Mullinax B, Lo J.
  TITLE     pDual-GC, a vector used in Stratagene's Expression Tested clones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6639)
  AUTHORS   Mullinax B, Lo J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2000) Technical Services, Stratagene, 11011 North 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 6639)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6639)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-APR-2000) Technical Services, Stratagene, 11011 North Torrey 
            Pines Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..123
                     /label=AmpR promoter
     promoter        127..484
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             519..1310
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    1545..1592
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1921..2509
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    complement(2618..2639)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2655..3734)
                     /label=lacI
                     /note="lac repressor"
     enhancer        3863..4166
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4167..4370
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        4399..4417
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4418..4442
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      4467..4476
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        4491..4595
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4596..5453
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             5530..5547
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             5554..5643
                     /label=3xMyc
                     /note="3 tandem Myc epitope tags"
     CDS             5674..5691
                     /label=6xHis
                     /note="6xHis affinity tag"
     protein_bind    5767..5791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     polyA_signal    complement(5899..6031)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(6178..6633)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agaatcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
      181 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
      241 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
      301 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      361 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
      421 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      481 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
      541 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
      601 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
      661 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
      721 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
      781 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
      841 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
      901 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
      961 tggaagccgg tcttgtcgat caggatgatc tggacgaaga acatcagggg ctcgcgccag
     1021 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     1081 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     1141 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     1201 ttgctgaaga acttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     1261 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     1321 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     1381 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     1441 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     1501 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     1561 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     1621 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     1681 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     1741 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     1801 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     1861 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1921 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1981 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2041 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     2101 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2161 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2221 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2281 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2341 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2401 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2461 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2521 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2581 gttatcccct gattctgtgg ataaccgtat taccgtaatg agtgagctaa cttacattaa
     2641 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     2701 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt
     2761 ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc
     2821 agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac
     2881 ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca
     2941 ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg
     3001 gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa
     3061 ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg
     3121 agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct
     3181 aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg
     3241 tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac
     3301 gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag
     3361 ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct
     3421 tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga
     3481 gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg
     3541 ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc
     3601 agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg
     3661 ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac
     3721 gttactggtt tcacattcac caccctgaat tgactctctt tcgggcgcta tcatgccata
     3781 ccgcgaaagg ttttgcgcca ttcgattatt aatagtaatc aattacgggg tcattagttc
     3841 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     3901 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     3961 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     4021 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     4081 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     4141 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     4201 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     4261 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     4321 cgcaaatggg cggtaggcgt gcctaatggg aggtctatat aagcagagct ggtttagtga
     4381 accgtcagat ccgctagcta atacgactca ctatagggga attgtgagcg gataacaatt
     4441 ccccttgttt aaactttaag aggagggcca ccatgggaag agaattcgtg cgcggaaccc
     4501 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4561 gataaatgct tcaataatat tgaaaaagga ggagtatgag tattcaacat ttccgtgtcg
     4621 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4681 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4741 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4801 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4861 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4921 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4981 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5041 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5101 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5161 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5221 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5281 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5341 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5401 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5461 cagaccaagt ttactcatat atagaattca agcttctctt cacttagttt aaacactgcg
     5521 gccgcgctgc tggttccgcg tggttctact agtgagcaga aactcatctc tgaagaagat
     5581 ctggaacaaa agttgatttc agaagaagat ctggaacaga agctcatctc tgaggaagat
     5641 ctgggtaccg ctgcgtccgc tgctggttct agacatcacc atcaccatca ctaatgataa
     5701 tccgcgtggt ttccaatacc cagctttgac ttgacttgat aacaggtaag tgtacccggg
     5761 atccggggaa ttgtgagcgg ataacaattc cccgatcccc ttcccaacag ttgcgcagcc
     5821 tgaatggcga atggagatcc aatttttaag tgtataatgt gttaaactac tgattctaat
     5881 tgtttgtgta ttttagattc cagacatgat aagatacatt gatgagtttg gacaaaccac
     5941 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     6001 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     6061 tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     6121 taacgcgtat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gacgcgtaaa
     6181 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     6241 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     6301 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     6361 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     6421 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     6481 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     6541 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     6601 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtg
//
