LOCUS       40924_15605        3179 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDSM0CD_Ttal1_TER40, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Young EM, Zhao Z, Gielesen BE, Wu L, Benjamin Gordon D, Roubos JA, 
            Voigt CA.
  TITLE     Iterative algorithm-guided design of massive strain libraries, 
            applied to itaconic acid production in yeast
  JOURNAL   Metab. Eng. 48, 33-43 (2018)
  PUBMED    29753070
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Young E, Zhao Z, Gielesen B, Wu L, Gordon DB, Roubos J, Voigt C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2018) Genetics, DSM Biotechnology Center, 
            Alexander Fleminglaan 1, Delft, South Holland 2613AX, Netherlands
REFERENCE   3  (bases 1 to 3179)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3179)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            48, 33-43 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-MAY-2018) Genetics, DSM Biotechnology Center, Alexander 
            Fleminglaan 1, Delft, South Holland 2613AX, Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     790..806
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2035..2126
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2127..2984
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3111..3179
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
       61 gcagatacca aatactgttc ttctagtgta gccgtagtta gcccaccact tcaagaactc
      121 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
      181 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
      241 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
      301 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
      361 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      421 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      481 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
      541 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      601 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
      661 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ggcgagagta gggaactgcc
      721 aggcatcaaa ctaagcagaa ggcccctgac ggatggcctt tttgcgtttc tacaaactct
      781 ttctgtgttg taaaacgacg gccagtctta agctcgggcc ccctgggcgg ttctgataac
      841 gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt tttatggggg
      901 gagtttaggg aaagagcatt tgtcagaata tttaagggcg cctgtcactt tgcttgatat
      961 atgagaatta tttaacctta taaatgagaa aaaagcaacg cactttaaat aagatacgtt
     1021 gctttttcga ttgatgaaca cctataatta aactattcat ctattattta tgattttttg
     1081 tatatacaat atttctagtt tgttaaagag aattaagaaa ataaatctcg aaaataataa
     1141 agggaaaatc agtttttgat atcaaaatta tacatgtcaa cgataataca aaatataata
     1201 caaactataa gatgttatca gtatttatta tgcatttaga atacgtactc agcgtctggg
     1261 ttccccatcg gtgatgtcgt ataagagacg tataggagac ctatagtgtc ttctatagcg
     1321 cgcaggtgtg tcgcccttcg ctgaaggtct cgtaaaggaa gtatctcgga aatattaatt
     1381 taggccatgt ccttatgcac gtttcttttg atacttacgg gtacatgtac acaagtatat
     1441 ctatatatat aaattaatga aaatccccta tttatatata tgactttaac gagacagaac
     1501 agttttttat tttttatcct atttgatgaa tgatacagtt tcttattcac gtgttatacc
     1561 cacaccaaat ccaatagcaa taccggccat cacaatcact gtttcggcag cccctaagat
     1621 cagacaaaac atccggaacc accttaaatc aacgtccctc ggagacccgt caaaagggcg
     1681 acacgaagac catatacgtc tcctataggt ctcgtataac ctgcttccta tacgacatca
     1741 ccgatgggga acacgaagcg atccagcccc cctaattagc ccgggcgaaa ggcccagtct
     1801 ttcgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg catggggagt
     1861 ccccacacta ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg
     1921 gaccaccgcg ctactgccgc caggcaaaca aggggtgtta tgagccatat tcaggtataa
     1981 atgggctcgc gataatgttc agaattggtt aattggttgt aacactgacc cctatttgtt
     2041 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2101 ttcaataata ttgaaaaagg aagaatatga gtattcaaca tttccgtgtc gcccttattc
     2161 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2221 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2281 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2341 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     2401 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2461 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2521 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2581 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2641 caaacgacga gcgtgacacc acgatgcctg tagcgatggc aacaacgttg cgcaaactat
     2701 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2761 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2821 aatccggagc cggtgagcgt ggttctcgcg gtatcatcgc agcgctgggg ccagatggta
     2881 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2941 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaaaag cagagcatta
     3001 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgc
     3061 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggt
//
