LOCUS       40924_15245        5976 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDSG310, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Glass DS, Riedel-Kruse IH.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming 
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Glass DS.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318 
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 5976)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5976)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell (2018)
            In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JUN-2018) Bioengineering, Stanford University, 318 Campus Drive,
            Clark Center E350, Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..879)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        906..1190
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     misc_feature    1219..1230
                     /label=RBS (BBa_B0034)
                     /note="RBS (BBa_B0034)"
     misc_feature    1219..1230
                     /label=BBa_J04500
                     /note="BBa_J04500"
     RBS             1219..1230
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1237..3225
                     /codon_start=1
                     /product="mutated Neae"
                     /label=mutated Neae
                     /note="removed restriction sites"
                     /protein_id="AXC07669.1"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENYF
                     KLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMMKA
                     APGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDDKAL
                     NYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQSGNN
                     FDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNVFIDQD
                     FSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRFNGYLPS
                     YPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYRHGTGNEN
                     DLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKKQDILSLNI
                     PHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQDYQAILPAY
                     VQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTADKTSAKADNAD
                     TITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTLKSSTPGQVVVS
                     AKTAEMTSALNASAVIFFDGATREPEA"
     CDS             3211..3225
                     /codon_start=1
                     /product="EPEA peptide protein"
                     /label=EPEA peptide protein
                     /note="C-terminus of alpha synuclein"
                     /protein_id="AXC07671.1"
                     /translation="EPEA"
     misc_feature    3223..3228
                     /label=stop codons
                     /note="stop codons"
     misc_feature    3229..3249
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      3250..3307
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    complement(3385..3402)
                     /label=VR primer site
                     /note="VR primer site"
     CDS             complement(3468..4280)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(4875..5419)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     misc_feature    5837..5857
                     /label=VF2 primer site
                     /note="VF2 primer site"
     terminator      complement(5909..5952)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    5955..5976
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga ttactcatgg ttgttatacc
     1261 cggacccggc acaagcataa gctaaaaaaa acattgatta tgcttagtgc tggtttagga
     1321 ttgttttttt atgttaatca gaactcattt gcaaatggtg aaaattattt taaattgggt
     1381 tcggattcaa aactgttaac tcatgatagc tatcagaatc gcctttttta tacgttgaaa
     1441 actggtgaaa ctgttgccga tctttctaaa tcgcaagata ttaatttatc gacgatttgg
     1501 tcgttgaata agcatttata cagttctgaa agcgaaatga tgaaggccgc gcctggtcag
     1561 cagatcattt tgccactcaa aaaacttccc tttgaataca gtgcactacc acttttaggt
     1621 tcggcacctc ttgttgctgc gggtggtgtt gctggtcaca cgaataaact gactaaaatg
     1681 tccccggacg tgaccaaaag caacatgacc gatgacaagg cattaaatta tgcggcacaa
     1741 caggcggcga gtctcggtag ccagcttcag tcgcgatctc tgaacggcga ttacgcgaaa
     1801 gataccgctc ttggtatcgc tggtaaccag gcttcgtcac agttgcaggc ctggttacaa
     1861 cattatggaa cggcagaggt taatctgcaa agtggtaata actttgacgg tagttcactg
     1921 gacttcttat taccgttcta tgattccgaa aaaatgctgg catttggtca ggtcggagcg
     1981 cgttacattg actcccgctt tacggcaaat ttaggtgcgg gtcagcgttt tttccttcct
     2041 gcaaacatgt tgggctataa cgtcttcatt gatcaggatt tttctggtga taatacccgt
     2101 ttaggtattg gtggcgaata ctggcgagac tatttcaaaa gtagcgttaa cggctatttc
     2161 cgcatgagcg gctggcatga gtcatacaat aagaaagact atgatgagcg cccagcaaat
     2221 ggcttcgata tccgttttaa tggctatcta ccgtcatatc cggcattagg cgccaagctg
     2281 atatatgagc agtattatgg tgataatgtt gctttgttta attctgataa gctgcaatcg
     2341 aatcctggtg cggcgaccgt tggtgtaaac tatactccga ttcctctggt gacgatgggg
     2401 atcgattacc gtcatggtac gggtaatgaa aatgatctcc tttactcaat gcagttccgt
     2461 tatcagtttg ataaatcgtg gtctcagcaa attgaaccac agtatgttaa cgagttaaga
     2521 acattatcag gcagccgtta cgatctggtt cagcgtaata acaatattat tctggagtac
     2581 aagaagcagg atattctttc tctgaatatt ccgcatgata ttaatggtac tgaacacagt
     2641 acgcagaaga ttcagttgat cgttaagagc aaatacggtc tggatcgtat cgtctgggat
     2701 gatagtgcat tacgcagtca gggcggtcag attcagcata gcggaagcca aagcgcacaa
     2761 gactaccagg ctattttgcc tgcttatgtg caaggtggca gcaatattta taaagtgacg
     2821 gctcgcgcct atgaccgtaa tggcaatagc tctaacaatg tacagcttac tattaccgtt
     2881 ctgtcgaatg gtcaagttgt cgaccaggtt ggggtaacgg actttacggc ggataagact
     2941 tcggctaaag cggataacgc cgataccatt acttataccg cgacggtgaa aaagaatggg
     3001 gtagctcagg ctaatgtccc tgtttcattt aatattgttt caggaactgc aactcttggg
     3061 gcaaatagtg ccaaaacgga tgctaacggt aaggcaaccg taacgttgaa gtcgagtacg
     3121 ccaggacagg tcgtcgtgtc tgctaaaacc gcggagatga cttcagcact taatgccagt
     3181 gcggttatat tttttgatgg tgcgactaga gaaccagagg cttaataata ctagtagcgg
     3241 ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc
     3301 gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt
     3361 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atctcgagtc ccgtcaagtc
     3421 agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg
     3481 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     3541 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     3601 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     3661 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     3721 ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     3781 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     3841 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     3901 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     3961 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     4021 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     4081 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     4141 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     4201 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctcga gcaagacgtt
     4261 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     4321 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     4381 acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt
     4441 cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc
     4501 tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt
     4561 caggcctggt atgagtcagc aacaccttct tcacgaggca gacctcagcg ctagcggagt
     4621 gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag
     4681 gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc
     4741 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga
     4801 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc
     4861 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
     4921 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tcccctggcg
     4981 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
     5041 ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
     5101 ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat
     5161 cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat
     5221 tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag
     5281 ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag
     5341 aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg
     5401 cagaccaaaa cgatctcaag aagatcatct tattaagggg tctgacgctc agtggaacga
     5461 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5521 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5581 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5641 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5701 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5761 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5821 ccagtctatt ccatggtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     5881 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
     5941 aaggatgatt tctggaattc gcggccgctt ctagag
//
