LOCUS       40924_15215        3543 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDSG264, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Glass DS, Riedel-Kruse IH.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming 
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Glass DS.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318 
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 3543)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3543)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell (2018)
            In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JUN-2018) Bioengineering, Stanford University, 318 Campus Drive,
            Clark Center E350, Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2..59
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(132..154)
                     /label=VR primer site(1)
                     /note="VR primer site(1)"
     misc_feature    complement(137..154)
                     /label=VR primer site
                     /note="VR primer site"
     CDS             complement(220..1032)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(1627..2171)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     misc_feature    2589..2609
                     /label=VF2 primer site
                     /note="VF2 primer site"
     terminator      complement(2661..2704)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2707..2728
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     misc_feature    2729..2777
                     /label=pLambda (BBa_R0051)
                     /note="pLambda (BBa_R0051)"
     misc_feature    2786..2797
                     /label=RBS (BBa_B0034)
                     /note="RBS (BBa_B0034)"
     RBS             2786..2797
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2804..3517
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     misc_feature    3524..3543
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
       61 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      121 ggcgagcggt atcagctcac tcaaaggcgg taatctcgag tcccgtcaag tcagcgtaat
      181 gctctgccag tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa
      241 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
      301 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
      361 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
      421 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
      481 cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
      541 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
      601 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
      661 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
      721 tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
      781 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
      841 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
      901 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
      961 atataaatca gcatccatgt tggaatttaa tcgcggcctc gagcaagacg tttcccgttg
     1021 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     1081 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct
     1141 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa
     1201 cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa
     1261 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg
     1321 gtatgagtca gcaacacctt cttcacgagg cagacctcag cgctagcgga gtgtatactg
     1381 gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa
     1441 aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca
     1501 ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg
     1561 gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa
     1621 gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca
     1681 gtggtggcga aacccgacag gactataaag ataccaggcg tttcccctgg cggctccctc
     1741 gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt
     1801 ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg
     1861 tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga
     1921 gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta attgatttag
     1981 aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg
     2041 actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg
     2101 aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa
     2161 aacgatctca agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaactcac
     2221 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2281 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2341 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2401 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2461 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2521 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2581 ttccatggtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     2641 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga
     2701 tttctggaat tcgcggccgc ttctagagta acaccgtgcg tgttgactat tttacctctg
     2761 gcggtgataa tggttgctac tagagaaaga ggagaaatac tagatgcgta aaggcgaaga
     2821 gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa
     2881 gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt
     2941 catctgtact actggtaaac tgccggtacc ttggccgact ctggtaacga cgctgactta
     3001 tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact tcttcaagtc
     3061 cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg acggcacgta
     3121 caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca ttgagctgaa
     3181 aggcattgac tttaaagaag acggcaatat cctgggccat aagctggaat acaattttaa
     3241 cagccacaat gtttacatca ccgccgataa acaaaaaaat ggcattaaag cgaattttaa
     3301 aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc agcaaaacac
     3361 tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagca cgcaaagcgt
     3421 tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc
     3481 agcgggcatc acgcatggta tggatgaact gtacaaatga tgatactagt agcggccgct
     3541 gca
//
