LOCUS       40924_15210        4131 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDSG263, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Glass DS, Riedel-Kruse IH.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming 
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Glass DS.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318 
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 4131)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4131)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell (2018)
            In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JUN-2018) Bioengineering, Stanford University, 318 Campus Drive,
            Clark Center E350, Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..49
                     /label=pLambda (BBa_R0051)
                     /note="pLambda (BBa_R0051)"
     misc_feature    58..69
                     /label=RBS (BBa_B0034)
                     /note="RBS (BBa_B0034)"
     RBS             58..69
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             76..789
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     misc_feature    793..813
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      814..873
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(1179..2126)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     misc_feature    complement(2130..2142)
                     /label=RBS
                     /note="RBS"
     regulatory      complement(2147..2166)
                     /label=repA promoter
                     /note="repA promoter"
                     /regulatory_class="promoter"
     rep_origin      complement(2174..2396)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2888..2982)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3015..3872)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3873..3977)
                     /label=AmpR promoter
     terminator      complement(4064..4107)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    4110..4131
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgcg taaaggcgaa gagctgttca ctggtgtcgt ccctattctg
      121 gtggaactgg atggtgatgt caacggtcat aagttttccg tgcgtggcga gggtgaaggt
      181 gacgcaacta atggtaaact gacgctgaag ttcatctgta ctactggtaa actgccggta
      241 ccttggccga ctctggtaac gacgctgact tatggtgttc agtgctttgc tcgttatccg
      301 gaccatatga agcagcatga cttcttcaag tccgccatgc cggaaggcta tgtgcaggaa
      361 cgcacgattt cctttaagga tgacggcacg tacaaaacgc gtgcggaagt gaaatttgaa
      421 ggcgataccc tggtaaaccg cattgagctg aaaggcattg actttaaaga agacggcaat
      481 atcctgggcc ataagctgga atacaatttt aacagccaca atgtttacat caccgccgat
      541 aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc acaacgtgga ggatggcagc
      601 gtgcagctgg ctgatcacta ccagcaaaac actccaatcg gtgatggtcc tgttctgctg
      661 ccagacaatc actatctgag cacgcaaagc gttctgtcta aagatccgaa cgagaaacgc
      721 gatcatatgg ttctgctgga gttcgtaacc gcagcgggca tcacgcatgg tatggatgaa
      781 ctgtacaaat gatactagta gcggccgctg cagtccggca aaaaaacggg caaggtgtca
      841 ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg
      901 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      961 gcggtaatct cgaggttaca ttgtcgatct gttcatggtg aacagcttta aatgcaccaa
     1021 aaactcgtaa aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatgg
     1081 acagttttcc ctttgatatc taacggtgaa cagttgttct acttttgttt gttagtcttg
     1141 atgcttcact gatagataca agagccataa gaacctcaga tccttccgta tttagccagt
     1201 atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc attgagatca
     1261 tgcttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg aatttttgca
     1321 gttaaagcat cgtgtagtgt ttttcttagt ccgttacgta ggtaggaatc tgatgtaatg
     1381 gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc ggttacgaga
     1441 tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg gcctcgctta
     1501 tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg tttcaaaacc
     1561 cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat gaacttaaat
     1621 tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag ttcttttaat
     1681 aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg ttccagatta
     1741 tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact aaaaactaat
     1801 tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc aaagccttta
     1861 accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc tttagctaat
     1921 acaccataag cattttccct actgatgttc atcatctgag cgtattggtt ataagtgaac
     1981 gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag tgccacacag
     2041 cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc tagttcattt
     2101 gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt ttaatcacta
     2161 taccaattga gatgggctag tcaatgataa ttacatgtcc ttttcctttg agttgtgggt
     2221 atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag accctctgta
     2281 aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat aatttataga
     2341 ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta taactcacta
     2401 ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa
     2461 aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggg caaatcgctg
     2521 aatattcctt ttgtctccga ccatcaggca cctgagtcgc tgtctttttc gtgacattca
     2581 gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt
     2641 atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacgg gcttctcagg
     2701 gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc agcagttcct
     2761 gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca ttcagactgg
     2821 ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta ctgtccctag
     2881 tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
     2941 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgtaaact
     3001 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3061 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3121 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3181 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3241 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3301 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3361 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3421 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3481 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3541 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3601 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3661 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3721 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3781 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3841 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     3901 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3961 caaatagggg ttccgcgcac atttcccatg gtgccacctg acgtctaaga aaccattatt
     4021 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
     4081 tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga g
//
