LOCUS       40924_15200        4149 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDSG261, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Glass DS, Riedel-Kruse IH.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming 
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Glass DS.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318 
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 4149)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4149)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell (2018)
            In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JUN-2018) Bioengineering, Stanford University, 318 Campus Drive,
            Clark Center E350, Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..49
                     /label=pLambda (BBa_R0051)
                     /note="pLambda (BBa_R0051)"
     misc_feature    58..69
                     /label=RBS (BBa_B0034)
                     /note="RBS (BBa_B0034)"
     RBS             58..69
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             76..786
                     /label=mRuby2
                     /note="monomeric red fluorescent protein derived from
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
     CDS             787..804
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    811..831
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      832..891
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(1197..2144)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     misc_feature    complement(2148..2160)
                     /label=RBS
                     /note="RBS"
     regulatory      complement(2165..2184)
                     /label=repA promoter
                     /note="repA promoter"
                     /regulatory_class="promoter"
     rep_origin      complement(2192..2414)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2906..3000)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3033..3890)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3891..3995)
                     /label=AmpR promoter
     terminator      complement(4082..4125)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    4128..4149
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggt gtctaagggc gaagagctga tcaaggaaaa tatgcgtatg
      121 aaggtggtca tggaaggttc ggtcaacggc caccaattca aatgcacagg tgaaggagaa
      181 ggcaatccgt acatgggaac tcaaaccatg aggatcaaag tcatcgaggg aggacccctg
      241 ccatttgcct ttgacattct tgccacgtcg ttcatgtatg gcagccgtac ttttatcaag
      301 tacccgaaag gcattcctga tttctttaaa cagtcctttc ctgagggttt tacttgggaa
      361 agagttacga gatacgaaga tggtggagtc gtcaccgtca tgcaggacac cagccttgag
      421 gatggctgtc tcgtttacca cgtccaagtc agaggggtaa actttccctc caatggtccc
      481 gtgatgcaga agaagaccaa gggttgggag cctaatacag agatgatgta tccagcagat
      541 ggtggtctga ggggatacac tcatatggca ctgaaagttg atggtggtgg ccatctgtct
      601 tgctctttcg taacaactta caggtcaaaa aagaccgtcg ggaacatcaa gatgcccggt
      661 atccatgccg ttgatcaccg cctggaaagg ttagaggaaa gtgacaatga aatgttcgta
      721 gtacaacgcg aacacgcagt tgccaagttc gccgggcttg gtggtgggat ggacgagctg
      781 tacaagcatc accaccacca tcactaatga tactagtagc ggccgctgca gtccggcaaa
      841 aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc
      901 gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      961 gtatcagctc actcaaaggc ggtaatctcg aggttacatt gtcgatctgt tcatggtgaa
     1021 cagctttaaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt tttacaccgt
     1081 tttcatctgt gcatatggac agttttccct ttgatatcta acggtgaaca gttgttctac
     1141 ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc
     1201 cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag
     1261 aacgaaccat tgagatcatg cttactttgc atgtcactca aaaattttgc ctcaaaactg
     1321 gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttacgtagg
     1381 taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc
     1441 tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca
     1501 gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta
     1561 cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc
     1621 attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt
     1681 tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac
     1741 ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc
     1801 tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg
     1861 aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct
     1921 ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat catctgagcg
     1981 tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg
     2041 ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga
     2101 ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct
     2161 aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt acatgtcctt
     2221 ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat
     2281 tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa
     2341 gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc
     2401 cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg
     2461 ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca
     2521 agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg
     2581 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg
     2641 gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac aagaaaagcc
     2701 cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta tctgactttt
     2761 tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga ttatcccgtg
     2821 acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca acaggcttac
     2881 ccgtcttact gtccctagtg cttggattct caccaataaa aaacgcccgg cggcaaccga
     2941 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     3001 caagcgagct cgtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3061 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3121 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3181 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3241 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3301 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3361 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3421 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3481 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3541 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3601 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3661 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3721 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3781 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3841 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3901 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3961 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttcccatggt gccacctgac
     4021 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
     4081 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
     4141 cttctagag
//
