LOCUS       40924_15175        3851 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDRIVE, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Rega S.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2006) Qiagen, 27220 Turnberry Lane, Suite 200, 
            Valencia, CA 91355, USA
REFERENCE   2  (bases 1 to 3851)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3851)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (18-SEP-2006) Qiagen, 27220 Turnberry Lane, Suite 200, Valencia, CA 
            91355, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        239..257
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    266..393
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(399..417)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(431..447)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(451..467)
                     /label=M13 forward (-40)
                     /note="M13 forward (-40)"
     rep_origin      complement(588..1043)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1070..1174
                     /label=AmpR promoter
     CDS             1175..2032
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             2181..2993
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3081..3669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta
      241 atacgactca ctatagggaa agctcggtac cacgcatgct gcagacgcgt tacgtatcgg
      301 atccagaatt cgtgatatct gaattcgtcg acaagcttct cgagcctagg ctagctctag
      361 accacacgtg tgggggcccg agctcgcggc cgctgtattc tatagtgtca cctaaatggc
      421 cgcacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      481 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      541 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt
      601 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
      661 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
      721 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
      781 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
      841 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
      901 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
      961 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     1021 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     1081 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1141 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1201 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1261 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1321 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1381 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     1441 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1501 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1561 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1621 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1681 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1741 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1801 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1861 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1921 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1981 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2041 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2101 gatctaggtg aagatccttt ttgataatct catgaacaat aaaactgtct gcttacataa
     2161 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tctaggccgc
     2221 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     2281 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc
     2341 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     2401 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     2461 catggttact caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc
     2521 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     2581 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     2641 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     2701 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
     2761 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     2821 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     2881 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     2941 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaagaat
     3001 taattcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     3061 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3121 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3181 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3241 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3301 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3361 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3421 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3481 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3541 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3601 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3661 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3721 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3781 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3841 gaagcggaag a
//
