LOCUS       62056_8580        7127 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7127)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2416..2433
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             2437..2469
                     /codon_start=1
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
                     /translation="MASMTGGQQMG"
     CDS             2473..2496
                     /codon_start=1
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
                     /translation="DLYDDDDK"
     CDS             2527..5571
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     rep_origin      5952..6540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        6626..6673
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             6693..7064
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 cctgcagggc ccactagttc catgtcctta tatggactca tctttgccta ttgcgacaca
       61 cactcaatga acacctacta cgcgctgcaa agagccccgc aggcctgagg tgcccccacc
      121 tcaccactct tcctattttt gtgtaaaaat ccagcttctt gtcaccacct ccaaggaggg
      181 ggaggaggag gaaggcaggt tcctctaggc tgagccgaat gcccctctgt ggtcccacgc
      241 cactgatcgc tgcatgccca ccacctgggt acacacagtc tgtgattccc ggagcagaac
      301 ggaccctgcc cacccggtct tgtgtgctac tcagtggaca gacccaaggc aagaaagggt
      361 gacaaggaca gggtcttccc aggctggctt tgagttccta gcaccgcccc gcccccaatc
      421 ctctgtggca catggagtct tggtccccag agtcccccag cggcctccag atggtctggg
      481 agggcagttc agctgtggct gcgcatagca gacatacaac ggacggtggg cccagaccca
      541 ggctgtgtag acccagcccc cccgccccgc agtgcctagg tcacccacta acgccccagg
      601 cctggtcttg gctgggcgtg actgttaccc tcaaaagcag gcagctccag ggtaaaaggt
      661 gccctgccct gtagagccca ccttccttcc cagggctgcg gctgggtagg tttgtagcct
      721 tcatcacggg ccacctccag ccactggacc gctggcccct gccctgtcct ggggagtgtg
      781 gtcctgcgac ttctaagtgg ccgcaagcca cctgactccc ccaacaccac actctacctc
      841 tcaagcccag gtctctccct agtgacccac ccagcacatt tagctagctg agccccacag
      901 ccagaggtcc tcaggccctg ctttcagggc agttgctctg aagtcggcaa gggggagtga
      961 ctgcctggcc actccatgcc ctccaagagc tccttctgca ggagcgtaca gaacccaggg
     1021 ccctggcacc cgtgcagacc ctggcccacc ccacctgggc gctcagtgcc caagagatgt
     1081 ccacacctag gatgtcccgc ggtgggtggg gggcccgaga gacgggcagg ccgggggcag
     1141 gcctggccat gcggggccga accgggcact gcccagcgtg gggcgcgggg gccacggcgc
     1201 gcgcccccag cccccgggcc cagcacccca aggcggccaa cgccaaaact ctccctcctc
     1261 ctcttcctca atctcgctct cgctcttttt ttttttcgca aaaggagggg agagggggta
     1321 aaaaaatgct gcactgtgcg gcgaagccgg tgagtgagcg gcgcggggcc aatcagcgtg
     1381 cgccgttccg aaagttgcct tttatggctc gagcggccgc ggcggcgccc tataaaaccc
     1441 agcggcgcga cgcgccacca ccgccgagac cgcgtccgcc ccgcgagcac agagcctcgc
     1501 ctttgccgat ccgccgcccg tccacacccg ccgccaggta agcccggcca gccgaccggg
     1561 gcaggcggct cacggcccgg ccgcaggcgg ccgcggcccc ttcgcccgtg cagagccgcc
     1621 gtctgggccg cagcgggggg cgcatggggg gggaaccgga ccgccgtggg gggcgcggga
     1681 gaagcccctg ggcctccgga gatgggggac accccacgcc agttcggagg cgcgaggccg
     1741 cgctcgggag gcgcgctccg ggggtgccgc tctcggggcg ggggcaaccg gcggggtctt
     1801 tgtctgagcc gggctcttgc caatggggat cgcagggtgg gcgcggcgga gcccccgcca
     1861 ggcccggtgg gggctggggc gccattgcgc gtgcgcgctg gtcctttggg cgctaactgc
     1921 gtgcgcgctg ggaattggcg ctaattgcgc gtgcgcgctg ggactcaagg cgctaactgc
     1981 gcgtgcgttc tggggcccgg ggtgccgcgg cctgggctgg ggcgaaggcg ggctcggccg
     2041 gaaggggtgg ggtcgccgcg gctcccgggc gcttgcgcgc acttcctgcc cgagccgctg
     2101 gccgcccgag ggtgtggccg ctgcgtgcgc gcgcgccgac ccggcgctgt ttgaaccggg
     2161 cggaggcggg gctggcgccc ggttgggagg gggttggggc ctggcttcct gccgcgcgcc
     2221 gcggggacgc ctccgaccag tgtttgcctt ttatggtaat aacgcggccg gcccggcttc
     2281 ctttgtcccc aatctgggcg cgcgccggcg ccccctggcg gcctaaggac tcggcgcgcc
     2341 ggaagtggcc agggcggggg cgacctcggc tcacagcgcg cccggctatt ctcgcagctc
     2401 accatggggg gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag
     2461 caaatgggtc gggatctgta cgacgatgac gataaggtac ctaaggatca gcttggagtt
     2521 gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     2581 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     2641 ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca
     2701 gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc
     2761 ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc
     2821 attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca
     2881 tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt
     2941 aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt
     3001 ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg
     3061 atggtgctgc gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc
     3121 ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat
     3181 gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg
     3241 tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag
     3301 gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc
     3361 gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg
     3421 aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa
     3481 gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc
     3541 aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc
     3601 atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc
     3661 gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc
     3721 ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg
     3781 accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc
     3841 gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct
     3901 aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat
     3961 gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg
     4021 gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta
     4081 cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt
     4141 ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc
     4201 gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg
     4261 gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg
     4321 gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt
     4381 ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat
     4441 agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt
     4501 gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg
     4561 cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc
     4621 gcatggtcag aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc
     4681 agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat
     4741 ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca
     4801 cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt
     4861 gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg
     4921 gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg
     4981 gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg
     5041 aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt
     5101 accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc
     5161 cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat
     5221 cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat
     5281 accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat
     5341 ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa
     5401 ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc
     5461 gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa
     5521 ttacagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataatct
     5581 agtcgagaat tcgctagctc gacatgataa gatacattga tgagtttgga caaaccacaa
     5641 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     5701 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     5761 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     5821 aagcaagtaa aacctctaca aatgtggtag atccatttaa atgttaatta actagccatg
     5881 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5941 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6001 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6061 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6121 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6181 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6241 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6301 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6361 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6421 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6481 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6541 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6601 ttcttaatta aatttttcaa aagtagttga caattaatca tcggcatagt atatcggcat
     6661 agtataatac gactcactat aggagggcca tcatggccaa gttgaccagt gctgtcccag
     6721 tgctcacagc cagggatgtg gctggagctg ttgagttctg gactgacagg ttggggttct
     6781 ccagagattt tgtggaggat gactttgcag gtgtggtcag agatgatgtc accctgttca
     6841 tctcagcagt ccaggaccag gtggtgcctg acaacaccct ggcttgggtg tgggtgagag
     6901 gactggatga gctgtatgct gagtggagtg aggtggtctc caccaacttc agggatgcca
     6961 gtggccctgc catgacagag attggagagc agccctgggg gagagagttt gccctgagag
     7021 acccagcagg caactgtgtg cactttgtgg cagaggagca ggactgagga taagaattga
     7081 gtttcagaaa agggggcctg agtggcccct tttttcaact taattaa
//
