LOCUS       V007927                11038 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007927
VERSION     V007927
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11038)
  AUTHORS   Wojcik EA, Brzostek A, Bacolla A, Mackiewicz P, Vasquez KM,
            Korycka-Machala M, Jaworski A, Dziadek J.
  TITLE     Direct and inverted repeats elicit genetic instability by both
            exploiting and eluding DNA double-strand break repair systems in
            mycobacteria
  JOURNAL   PLoS ONE 7 (12), E51064 (2012)
   PUBMED   23251422
REFERENCE   2  (bases 1 to 11038)
  AUTHORS   Dziadek J, Wojcik EA.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2012) Pharmacology and Toxicology, University of
            Texas, 1400 Barbara Jordan Blvd., Austin, TX 78759, USA
REFERENCE   3  (bases 1 to 11038)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11038)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2012"; volume: "7"; issue: "12"; pages: "E51064"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-OCT-2012) Pharmacology and Toxicology, University of Texas, 1400
            Barbara Jordan Blvd., Austin, TX 78759, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             511..1623
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     CDS             1907..2719
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3054..3642
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4067..5014)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     promoter        5027..5055
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             5244..5774
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     repeat_region   5895..5932
                     /rpt_family="DR-2"
     promoter        6687..6705
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             6995..10039
                     /label="lacZ"
                     /note="beta-galactosidase"
     terminator      10956..11002
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagagccg tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct
       61 gcaactcccg gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg
      121 tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa
      181 agtagccaga tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag
      241 agttgcagac ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga
      301 gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac
      361 atatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
      421 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
      481 tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag
      541 actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac
      601 tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc gggcgagaag
      661 cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa ggcagccgcc
      721 agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga cctcgcagac
      781 ggcaccaggg atctgtacag cgggcacgcg gagcgccgca tctacccggt gctaggtgaa
      841 gtggcggtca cagagatgac gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg
      901 aagcacccga ctgcccgccg gcatgcctac aacgtcctcc gggcggtgat gaacacagcg
      961 gtcgaggaca agctgatcgc agagaacccg tgccggatcg agcagaaggc agccgatgag
     1021 cgcgacgtag aggcgctgac gcctgaggag ctggacatcg tcgccgctga gatcttcgag
     1081 cactaccgga tcgcggcata catcctggcg tggacgagcc tccggttcgg agagctgatc
     1141 gagcttcgcc gcaaggacat cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt
     1201 ggcgcttccc gcgtggggaa caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag
     1261 cgtcctgtga cggttccgcc tcacgtcgcg gagatgatcc gagcgcacat gaaggaccgt
     1321 acgaagatga acaagggccc cgaggcattc ctggtgacca cgacgcaggg caaccggctg
     1381 tcgaagtccg cgttcaccaa gtcgctgaag cgtggctacg ccaagatcgg tcggccggaa
     1441 ctccgcatcc acgacctccg cgctgtcggc gctacgttcg ccgctcaggc aggtgcgacg
     1501 accaaggagc tgatggcccg tctcggtcac acgactccta ggatggcgat gaagtaccag
     1561 atggcgtctg aggcccgcga cgaggctatc gctgaggcga tgtccaagct ggccaagacc
     1621 tcctgaaacg caaaaagccc ccctcccaag gacactgagt cctaaagagg ggggtttctt
     1681 gtcagtacgc gaagaaccac gcctggccgc gagcgccagc accgccgctc tgtgcggaga
     1741 cctgggcacc agccccgccg ccgccaggag cattgccgtt cccgccagct agcaacaaag
     1801 cgacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     1861 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     1921 acgggaaacg tcttgctcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     1981 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
     2041 gaagccccat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     2101 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     2161 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac
     2221 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     2281 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     2341 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     2401 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataatct
     2461 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     2521 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     2581 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     2641 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     2701 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
     2761 tacgctgact tgacgggacg gcggctttgt tgaataaatc gaacttttgc tgagttgaag
     2821 gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa
     2881 atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg
     2941 ctggatgatg gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga
     3001 cctcactagt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3061 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3121 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3181 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     3241 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3301 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3361 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3421 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     3481 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3541 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3601 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3661 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3721 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3781 cgaacgaccg agcgcaacgc gtgcggccgc ggtacccggg gatccttgcc gagctgggat
     3841 ggaagcccgg ccgacccacc ctggaggaga tgatcgagga tgccagggcc tttcacgccc
     3901 gccgctgctg agcgtccgcc gccgggcccg caccgccgtc ggccggcccg ctccgggctc
     3961 gcagcagcgg gcttcggcgc gggcccgggg ctcccgggcc gccgggcggg gctccgcccg
     4021 gcggccgccg ggggccgggg gcggcgccgg gcggcccggg gcgtcaggcg ccgggggcgg
     4081 tgtccggcgg cccccagagg aactgcgcca gttcctccgg atcggtgaag ccggagagat
     4141 ccagcggggt ctcctcgaac acctcgaagt cgtgcaggaa ggtgaaggcg agcagttcgc
     4201 gggcgaagtc ctcggtccgc ttccactgcg ccccgtcgag cagcgcggcc aggatctcgc
     4261 ggtcgccccg gaaggcgttg agatgcagtt gcaccaggct gtagcgggag tctcccgcat
     4321 agacgtcggt gaagtcgacg atcccggtga cctcggtcgc ggccaggtcc acgaagatgt
     4381 tggtcccgtg caggtcgccg tggacgaacc ggggttcgcg gccggccagc agcgtgtcca
     4441 cgtccggcag ccagtcctcc aggcggtcca gcagccgggg cgagaggtag ccccacccgc
     4501 ggtggtcctc gacggtcgcc gcgcggcgtt cccgcagcag ttccgggaag acctcggaat
     4561 ggggggtgag cacggtgttc ccggtcagcg gcaccctgtg cagccggccg agcacccggc
     4621 cgagttcgcg ggccagggcg agcagcgcgt tccggtcggt cgtgccgtcc atcgcggacc
     4681 gccaggtggt gccggtcatc cggctcatca ccaggtaggg ccacggccag gctccggtgc
     4741 cgggccgcag ctcgccgcgg ccgaggaggc ggggcaccgg caccggggcg tccgccagga
     4801 ccgcgtacgc ctccgactcc gacgcgaggc tctccggacc gcaccagtgc tcgccgaaca
     4861 gcttgatcac cgggtcgggc tcgccgacca gtacggggtt ggtgctctcg ccgggcaccc
     4921 gcagcaccgg cggcaccggc agcccgagct cctccagggc tcggcgggcc agcggctccc
     4981 agaattcctg gtcgttccgc agcaagcagt gggtaccgag ctcgaattga cataagcctg
     5041 ttcggttcgt aaactgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     5101 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     5161 ttttgtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     5221 tttgatgtta tggagcagca acgatgttac gcagcagcaa cgatgttacg cagcagggca
     5281 gtcgccctaa aacaaagtta ggtggctcaa gtatgggcat cattcgcaca tgtaggctcg
     5341 gccctgacca agtcaaatcc atgcgggctg ctcttgatct tttcggtcgt gagttcggag
     5401 acgtagccac ctactcccaa catcagccgg actccgatta cctcgggaac ttgctccgta
     5461 gtaagacatt catcgcgctt gctgccttcg accaagaagc ggttgttggc gctctcgcgg
     5521 cttacgttct gcccaggttt gagcagccgc gtagtgagat ctatatctat gatctcgcag
     5581 tctccggcga gcaccggagg cagggcattg ccaccgcgct catcaatctc ctcaagcatg
     5641 aggccaacgc gcttggtgct tatgtgatct acgtgcaagc agattacggt gacgatcccg
     5701 cagtggctct ctatacaaag ttgggcatac gggaagaagt gatgcacttt gatatcgacc
     5761 caagtaccgc cacctaacaa ttcgttcaag ccgagatcgg cttcccggcc gcggagttgt
     5821 tcggtaaatt gtcacaacgc cgcggccaat tcgagctcgg tacccactta ggtcaccgtt
     5881 gtttaaacat cgaacgggcc agcggctccc agaattcctg gtcgttccgc aggctcgcgt
     5941 aggaatcatc cgaatcaata cggtcgagaa gtaacaggga ttcttgtgtc acagcggacc
     6001 tctattcaca gggtacgggc cggcttaatt ccgcacggcc ggtcgcgaca cggcctgtcc
     6061 gcaccgcgga tcaggcgttg acgatgacgg gctggtcggc cacgtcgggg acgttctcgg
     6121 tggtgctgcg gtcgggatcg ccaatctcta cgggccgacc gaggcgacgg tgtacgccac
     6181 cgcctggttc tgcgacggcg aggcgccgtc ccaggccccg ccgatccccg tcccccgcgt
     6241 cgtcgagcgc ggtgccgacg acaccgccgc gtggctcgtc acggaggccg tccccggcgt
     6301 cgcggcggcc gaggagtggc ccgagcacca gcggttcgcc gtggtcgagg cgatggcgga
     6361 gctggcccgc gccctccacg agctgcccgt ggaggactgc cccttcgacc ggcgcctcga
     6421 cgcggcggtc gccgaggccc ggcggaacgt cgccgagggc ctgtggacct cgacgacctg
     6481 caggcatgca agcttatttc cgggtgcggg acggcgttac cagaaactca gaaggttcgt
     6541 ccaaccaaac cgactctgac ggcagtttac gagagagatg atagggtctg cttcagtaag
     6601 ccagatgcta cacaattagg cttgtacata ttgtcgttag aacgcggcta caattaatac
     6661 ataaccttat gtatcataca catacgattt aggtgacact atagaataca taggatctgg
     6721 ctgagctcca ccgcggtggc ggccgctcta gacgacacat gcccagacac tgcggaaatg
     6781 ccaccttcag gccgtcgcgt cggtcccgaa ttggccgtga acgaccgccg gataagggtt
     6841 tcggcggtgc gcttgatgcg ggtggacgcc cgaagttgtg gttgactaca cgagcactgc
     6901 cgggcccagc gcctgcagtc tgacctaatt caggatgcgc ccaaacatgc atggatgcgt
     6961 tgagatgagg atgagggaag caagaatgga tcccgtcgtt ttacaacgtc gtgactggga
     7021 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     7081 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     7141 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga
     7201 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc
     7261 gcccatctac accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga
     7321 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg
     7381 ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg
     7441 ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt
     7501 acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct
     7561 ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa
     7621 accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg
     7681 cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac
     7741 agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga
     7801 aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa
     7861 cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac
     7921 cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat
     7981 tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca
     8041 cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct
     8101 gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct
     8161 gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac
     8221 ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag
     8281 cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc
     8341 gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc
     8401 tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga
     8461 tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa
     8521 atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga
     8581 atacgcccac gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg
     8641 tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa
     8701 atatgatgaa aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa
     8761 cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct
     8821 gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga
     8881 agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc
     8941 gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa
     9001 acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac
     9061 agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg
     9121 gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat
     9181 cccgcatctg accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca
     9241 atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct
     9301 gacgccgctg cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga
     9361 agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca
     9421 ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac
     9481 gaccgctcac gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg
     9541 gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc
     9601 gcatccggcg cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg
     9661 gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg
     9721 ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct
     9781 gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt
     9841 caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca
     9901 cgcggaagaa ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga
     9961 ctcctggagc ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca
    10021 gttggtctgg tgtcaaaaat aataataacc gggcaggggg gatcccccct cgagtgacca
    10081 caacgacgcg cccgctttga tcggggacgt ctgcggccga ccatttacgg gtcttgttgt
    10141 cgttggcggt catgggccga acatactcac ccggatcgga gggccgagga caaggtcgaa
    10201 cgaggggcat gacccggtgc ggggcttctt gcactcggca taggcgagtg ctaagaataa
    10261 cgttggcact cgcgaccggt gagtcgtagg tcgggacggt gaggccaggc ccgtcgtcgc
    10321 agcgagtggc agcgaggaca acttgagccg tccgtcgcgg gcactgcgcc cggccagcgt
    10381 aagtagcggg gttgccgtca cccggtgact cgaggtcgat actttggcgt caccccttac
    10441 atattttagg tcttttttta ttgtgcgtaa ctaacttgcc atcttcaaac aggagggctg
    10501 gaagaagcag accgctaaca cagtacataa aaaaggagac atgaacgatg aacatcaaaa
    10561 agtttgcaaa acaagcaaca gtattaacct ttactaccgc actgctggca ggaggcgcaa
    10621 ctcaagcgtt tgcgcatatg aaagaaacga accaaaagcc atataaggaa acatacggca
    10681 tttcccatat tacacgccat gatatgctgc aaatccctga acagcaaaaa aatgaaaaat
    10741 atcaagttcc tgaattcgat tcgtccacaa ttaaaaatat ctcttctgca aaaggcctgg
    10801 acgtttggga cagctggcca ttacaaaacg ctgacggcac tgtcgcaaac tatcacggct
    10861 accacatcgt ctttgcatta gccggagatc ctaaaaatgc ggatgacaca tcgatgtcga
    10921 cgtagttaac tagcgtacga tcgactgcca ggcatcaaat aaaacgaaag gctcagtcga
    10981 aagactgggc ctttcgtttt atctgttgtt tgtccggcca tcatggccgc ggtgatca
//
