LOCUS       V007929                 6998 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007929
VERSION     V007929
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Wojcik EA, Brzostek A, Bacolla A, Mackiewicz P, Vasquez KM,
            Korycka-Machala M, Jaworski A, Dziadek J.
  TITLE     Direct and inverted repeats elicit genetic instability by both
            exploiting and eluding DNA double-strand break repair systems in
            mycobacteria
  JOURNAL   PLoS ONE 7 (12), E51064 (2012)
   PUBMED   23251422
REFERENCE   2  (bases 1 to 6998)
  AUTHORS   Dziadek J, Wojcik EA.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2012) Pharmacology and Toxicology, University of
            Texas, 1400 Barbara Jordan Blvd., Austin, TX 78759, USA
REFERENCE   3  (bases 1 to 6998)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6998)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2012"; volume: "7"; issue: "12"; pages: "E51064"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-OCT-2012) Pharmacology and Toxicology, University of Texas, 1400
            Barbara Jordan Blvd., Austin, TX 78759, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      complement(2028..3927)
                     /direction=LEFT
                     /label="mycobacterial OriM region from pAL5000"
                     /note="mycobacterial OriM region from pAL5000"
     CDS             complement(4441..5388)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     promoter        5401..5429
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             5618..6148
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     repeat_region   6269..6306
                     /rpt_family="DR-1"
     terminator      6916..6962
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagcaaca aagcgacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
       61 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      121 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
      181 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      241 atcgcttgta tgggaagccc catgcgccag agttgtttct gaaacatggc aaaggtagcg
      301 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
      361 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      421 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
      481 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
      541 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
      601 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
      661 aaatgcataa tcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
      721 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
      781 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
      841 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
      901 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
      961 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt
     1021 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     1081 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     1141 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     1201 ttcacgaggc agacctcact agttccactg agcgtcagac cccgtagaaa agatcaaagg
     1261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1381 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1621 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     1681 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1741 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1801 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1861 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1921 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1981 cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcacgcgtg agcccaccag
     2041 ctccgtaagt tcgggtgctg tgtggctcgt acccgcgcat tcaggcggca gggggtctaa
     2101 cgggtctaag gcggcgtgta cggccgccac agcggctctt agcggcccgg aaacgtcctc
     2161 gaaacgacgc atgtgttcct cctggttggt acaggtggtt gggggtgctc ggctgtcgct
     2221 ggtgtttcat catcagggct cgacgggaga gcgggggagt gtgcagttgt ggggtggccc
     2281 ctcagcgaaa tatctgactt ggagctcgtg tcggaccata caccggtgat taatcgtggt
     2341 ctactaccaa gcgtgagcca cgtcgccgac gaatttgagc agctctggct gccgtactgg
     2401 ccgctggcaa gcgacgatct gctcgagggg atctaccgcc aaagccgcgc gtcggcccta
     2461 ggccgccggt acatcgaggc gaacccaaca gcgctggcaa acctgctggt cgtggacgta
     2521 gaccatccag acgcagcgct ccgagcgctc agcgcccggg ggtcccatcc gctgcccaac
     2581 gcgatcgtgg gcaatcgcgc caacggccac gcacacgcag tgtgggcact caacgcccct
     2641 gttccacgca ccgaatacgc gcggcgtaag ccgctcgcat acatggcggc gtgcgccgaa
     2701 ggccttcggc gcgccgtcga tggcgaccgc agttactcag gcctcatgac caaaaacccc
     2761 ggccacatcg cctgggaaac ggaatggctc cactcagatc tctacacact cagccacatc
     2821 gaggccgagc tcggcgcgaa catgccaccg ccgcgctggc gtcagcagac cacgtacaaa
     2881 gcggctccga cgccgctagg gcggaattgc gcactgttcg attccgtcag gttgtgggcc
     2941 tatcgtcccg ccctcatgcg gatctacctg ccgacccgga acgtggacgg actcggccgc
     3001 gcgatctatg ccgagtgcca cgcgcgaaac gccgaatttc cgtgcaacga cgtgtgtccc
     3061 ggaccgctac cggacagcga ggtccgcgcc atcgccaaca gcatttggcg ttggatcaca
     3121 accaagtcgc gcatttgggc ggacgggatc gtggtctacg aggccacact cagtgcgcgc
     3181 cagtcggcca tctcgcggaa gggcgcagca gcgcgcacgg cggcgagcac agttgcgcgg
     3241 cgcgcaaagt ccgcgtcagc catgcatgga ggcattgcta tgagcgacgg ctacagcgac
     3301 ggctacagcg acggctacaa ccggcagccg actgtccgca aaaagcggcg cgtgaccgcc
     3361 gccgaaggcg ctcgaatcac cggactatcc gaacgccacg tcgtccggct cgtggcgcag
     3421 gaacgcagcg agtggctcgc cgagcaggct gcacgccgcg aacgcatccg cgcctatcac
     3481 gacgacgagg gccactcttg gccgcaaacg gccaaacatt tcgggctgca tctggacacc
     3541 gttaagcgac tcggctatcg ggcgaggaaa gagcgtgcgg cagaacagga agcggctcaa
     3601 aaggcccaca acgaagccga caatccaccg ctgttctaac gcaattgggg agcgggtgtc
     3661 gcgggggttc cgtggggggt tccgttgcaa cgggtcggac aggtaaaagt cctggtagac
     3721 gctagttttc tggtttgggc catgcctgtc tcgttgcgtg tttcgttgcg tccgttttga
     3781 ataccagcca gacgagacgg ggttctacga atcttggtcg ataccaagcc atttccgctg
     3841 aatatcgtgg agctcaccgc cagaatcggt ggttgtggtg atgtacgtgg cgaactccgt
     3901 tgtagtgctt gtggtggcat ccgtggcgcg gccgcggtac cagatcttta aatctagacg
     3961 acacatgccc agacactgcg gaaatgccac cttcaggccg tcgcgtcggt cccgaattgg
     4021 ccgtgaacga ccgccggata agggtttcgg cggtgcgctt gatgcgggtg gacgcccgaa
     4081 gttgtggttg actacacgag cactgccggg cccagcgcct gcagtctgac ctaattcagg
     4141 atgcgcccaa acatgcatgg atgcgttgag atgaggatga gggaagcaag aatggatcct
     4201 tgccgagctg ggatggaagc ccggccgacc caccctggag gagatgatcg aggatgccag
     4261 ggcctttcac gcccgccgct gctgagcgtc cgccgccggg cccgcaccgc cgtcggccgg
     4321 cccgctccgg gctcgcagca gcgggcttcg gcgcgggccc ggggctcccg ggccgccggg
     4381 cggggctccg cccggcggcc gccgggggcc gggggcggcg ccgggcggcc cggggcgtca
     4441 ggcgccgggg gcggtgtccg gcggccccca gaggaactgc gccagttcct ccggatcggt
     4501 gaagccggag agatccagcg gggtctcctc gaacacctcg aagtcgtgca ggaaggtgaa
     4561 ggcgagcagt tcgcgggcga agtcctcggt ccgcttccac tgcgccccgt cgagcagcgc
     4621 ggccaggatc tcgcggtcgc cccggaaggc gttgagatgc agttgcacca ggctgtagcg
     4681 ggagtctccc gcatagacgt cggtgaagtc gacgatcccg gtgacctcgg tcgcggccag
     4741 gtccacgaag atgttggtcc cgtgcaggtc gccgtggacg aaccggggtt cgcggccggc
     4801 cagcagcgtg tccacgtccg gcagccagtc ctccaggcgg tccagcagcc ggggcgagag
     4861 gtagccccac ccgcggtggt cctcgacggt cgccgcgcgg cgttcccgca gcagttccgg
     4921 gaagacctcg gaatgggggg tgagcacggt gttcccggtc agcggcaccc tgtgcagccg
     4981 gccgagcacc cggccgagtt cgcgggccag ggcgagcagc gcgttccggt cggtcgtgcc
     5041 gtccatcgcg gaccgccagg tggtgccggt catccggctc atcaccaggt agggccacgg
     5101 ccaggctccg gtgccgggcc gcagctcgcc gcggccgagg aggcggggca ccggcaccgg
     5161 ggcgtccgcc aggaccgcgt acgcctccga ctccgacgcg aggctctccg gaccgcacca
     5221 gtgctcgccg aacagcttga tcaccgggtc gggctcgccg accagtacgg ggttggtgct
     5281 ctcgccgggc acccgcagca ccggcggcac cggcagcccg agctcctcca gggctcggcg
     5341 ggccagcggc tcccagaatt cctggtcgtt ccgcagcaag cagtgggtac cgagctcgaa
     5401 ttgacataag cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg ctcacgcaac
     5461 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     5521 tgttatgact gtttttttgt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     5581 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gcaacgatgt
     5641 tacgcagcag ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg gcatcattcg
     5701 cacatgtagg ctcggccctg accaagtcaa atccatgcgg gctgctcttg atcttttcgg
     5761 tcgtgagttc ggagacgtag ccacctactc ccaacatcag ccggactccg attacctcgg
     5821 gaacttgctc cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag aagcggttgt
     5881 tggcgctctc gcggcttacg ttctgcccag gtttgagcag ccgcgtagtg agatctatat
     5941 ctatgatctc gcagtctccg gcgagcaccg gaggcagggc attgccaccg cgctcatcaa
     6001 tctcctcaag catgaggcca acgcgcttgg tgcttatgtg atctacgtgc aagcagatta
     6061 cggtgacgat cccgcagtgg ctctctatac aaagttgggc atacgggaag aagtgatgca
     6121 ctttgatatc gacccaagta ccgccaccta acaattcgtt caagccgaga tcggcttccc
     6181 ggccgcggag ttgttcggta aattgtcaca acgccgcggc caattcgagc tcggtaccca
     6241 cttaggtcac cgttgtttaa acatcgaacg ggccagcggc tcccagaatt cctggtcgtt
     6301 ccgcaggctc gcgtaggaat catccgaatc aatacggtcg agaagtaaca gggattcttg
     6361 tgtcacagcg gacctctatt cacagggtac gggccggctt aattccgcac ggccggtcgc
     6421 gacacggcct gtccgcaccg cggatcaggc gttgacgatg acgggctggt cggccacgtc
     6481 ggggacgttc tcggtggtgc tgcggtcggg atcgccaatc tctacgggcc gaccgaggcg
     6541 acggtgtacg ccaccgcctg gttctgcgac ggcgaggcgc cgtcccaggc cccgccgatc
     6601 cccgtccccc gcgtcgtcga gcgcggtgcc gacgacaccg ccgcgtggct cgtcacggag
     6661 gccgtccccg gcgtcgcggc ggccgaggag tggcccgagc accagcggtt cgccgtggtc
     6721 gaggcgatgg cggagctggc ccgcgccctc cacgagctgc ccgtggagga ctgccccttc
     6781 gaccggcgcc tcgacgcggc ggtcgccgag gcccggcgga acgtcgccga gggcctgtgg
     6841 acctcgacga cctgcaggca tgcaagctta tcgatgtcga cgtagttaac tagcgtacga
     6901 tcgactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     6961 atctgttgtt tgtccggcca tcatggccgc ggtgatca
//
