LOCUS       V016062                 5329 bp    DNA     circular SYN 10-FEB-2019
DEFINITION  Exported.
ACCESSION   V016062
VERSION     V016062
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Hua M.
  TITLE     A dual-replicon shuttle vector system for heterologous replication
            and expression in a broad range of gram-positive and negative
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Hua M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JAN-2018) Institute of Infectious Diseases, Beijing
            Ditan Hospital, No. 8 Jingshundongjie, Beijing 100015, China,
            Beijing, Beijing 100015, China
REFERENCE   3  (bases 1 to 5329)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-JAN-2018) Institute of Infectious Diseases, Beijing Ditan
            Hospital, No. 8 Jingshundongjie, Beijing 100015, China, Beijing,
            Beijing 100015, China"
FEATURES             Location/Qualifiers
     source          1..5329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      49..80
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        81..183
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             184..1041
                     /label="AmpR"
                     /note="beta-lactamase"
     terminator      1068..1095
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1107..1694)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(1716..1745)
                     /label="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     CDS             3181..3315
                     /gene="copG"
                     /label="Protein CopG"
                     /note="Protein CopG from Streptococcus agalactiae.
                     Accession#: P13920"
     CDS             3379..4011
                     /codon_start=1
                     /transl_table=11
                     /product="RepB"
                     /label="RepB"
                     /protein_id="QBA30996.1"
                     /translation="MAKEKARYFTFLLYPESIPSDWELKLETLGVPMAISPLHDKDKSS
                     IKGQKYKKAHYHVLYIAKNPVTADSVRKKIKLLLGEKSLAMVQVVLNVENMYLYLTHES
                     KDAIAKKKHVYDKADIKLINNFDIDRYVTLDVEEKTELFNVVVSLIRAYTLQNIFDLYD
                     FIDENGETYGLTINLVNEVIAGKTGFMKLLFDGAYQRSKRGTKNEER"
     CDS             4262..5245
                     /gene="malR"
                     /label="HTH-type transcriptional regulator MalR"
                     /note="HTH-type transcriptional regulator MalR from
                     Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 /
                     TIGR4). Accession#: P0A4T1"
ORIGIN
        1 atcgctcaat actgaccatt taaatcatac ctgacctcca tagcagaaag tcaaaagcct
       61 ccgaccggag gcttttgact tgatcggcac gtaagaggtt ccaactttca ccataatgaa
      121 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
      181 aaaatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      241 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      301 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      361 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      421 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      481 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
      541 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
      601 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
      661 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
      721 atgcctgtag cgatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
      781 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
      841 cgctcggccc ttccggctgg ctggtttatt gctgataaat ccggagccgg tgagcgtggt
      901 tctcgcggta tcatcgcagc gctggggcca gatggtaagc cctcccgtat cgtagttatc
      961 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1021 gcctcactga ttaagcattg gtaatgaggg cccaaatgta atcacctggc tcaccttcgg
     1081 gtgggccttt ctgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1141 aaaaatcgat gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1201 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1261 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1321 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1381 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1441 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1501 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1561 atctgcgctc tgctgaagcc agttacctcg gaaaaagagt tggtagctct tgatccggca
     1621 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1681 aaaaaggatc tcaagaagat cctttgattt tctaccgaag aaaggcccac ccgtgaaggt
     1741 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgatg
     1801 aattcaagct tggtggcggg taccggatcc gcggccgcgc tagcgagctc agatctaggc
     1861 ctgatccatc tctagagtat tttgcagacg caaacgtttg cgttacttat aagtatactc
     1921 cttttcaagc ataattgcaa gcgtttttaa aaaacttttt tgattatttt taaggagact
     1981 aactcatttt agcaaagata acttaaaact atctaatcct aaggtttgac tctcacgaga
     2041 cagtctacaa agtacaaaac ctccttaggg agcttgaatt tatatgtaac aaagcacaaa
     2101 cgtccataga aaatagatag ggattagaac cagggaggtc ttaaggcgat taagccaatc
     2161 gtgaatataa ttaaaaaggc tattgaaaag agcttgttct gagcgattta tgccgtgaaa
     2221 gctatttgac aataagcagt gacagagtac gctaggacgt gccgagccga aaggctttag
     2281 cgtttcggac ggacacggac aaaggacggc agtcactggt tacttgttgt caaatagacc
     2341 atggaataaa aagcgtcaaa agtcttgagt ggatgatacc ctatggtact ctattcgcct
     2401 tttgactttt ttgctataat ttaagtgtcg ccagttcttc cgtcaggtaa tgcgaactta
     2461 gactggaggt gagcgttgtg aagacattcc tcgagcttgt ctttgtccct tttgtggttg
     2521 gcgttgctgc agaagtagtc gctgattggc taattcagcg tatcaaagac aaaggcgacc
     2581 aaaaatagct tgagttcttt tagaacaaaa aagaaagaca gtagttgcac ctactgtctt
     2641 tcttttgcgt tgtgctttta gttcctcgaa cttttagcgt caagcatatt atatcatggg
     2701 gcgagaaatt ctgtcaaaat aatgctataa tgcttttgag gcacctcagc gatacggtcg
     2761 gtggtgtgaa tctcatttac gtagggcgac tggaaacgga tagctcaaag ggcgcgtttg
     2821 agtgtcgggt gtgggactgc cttcagcttc gggctgtaaa gacccctgat acttttgaat
     2881 gagatgaccc tttggggtct tttttgtttt tttagggaga tgttgtgggg gattttttct
     2941 ccgaaaaaat ctaaaatatg ggggggctac tacgaccccc cctatagtgc cgagtgccaa
     3001 aatcaaaaaa aaaacgcctt tagccttaga gctgcaaggg tttgaggctc gtcaaatctc
     3061 ggcgactttt cggcgacttt tcggcgactt tttagagatt ttttgggaaa aatacgaaaa
     3121 agattgcatt gagtgcacgg ttatgctact atagttttat aaaattttga gaggtgacgc
     3181 atgaaaaaaa gattgacgat aacattaagt gaatcggtac ttgaaaatct tgaaaaaatg
     3241 gcaagagaga tggggttatc aaaatctgca atgatttctg ttgccttgga aaattacaag
     3301 aaaggtcaag aaaaataaaa aaagccgtgc tggcaggcac tggctaaagt caaacatttc
     3361 ttgggtatat tatactttat ggctaaagaa aaagcaagat acttcacttt tttactttat
     3421 cctgaatcaa ttccaagcga ctgggagctg aaacttgaaa cgcttggagt gccgatggca
     3481 attagtccat tgcatgataa ggataagagt agtatcaaag gacaaaaata taagaaagct
     3541 cattatcatg tgctttatat agctaaaaat ccagttactg cagatagtgt acgtaaaaag
     3601 attaaattat tgcttggtga aaaaagtctt gcaatggtgc aggttgttct caatgtcgaa
     3661 aatatgtatt tgtatttaac gcacgagagc aaggacgcta ttgctaagaa gaaacatgtt
     3721 tatgataagg ctgatataaa gctaatcaat aattttgata ttgaccgtta tgtgacgtta
     3781 gatgtcgagg aaaagaccga acttttcaat gtggttgtat cgcttattcg tgcgtacact
     3841 ctccaaaata tttttgattt gtatgatttc attgacgaaa atggagaaac ttatgggttg
     3901 actataaatt tggttaacga agttattgca gggaaaactg gttttatgaa attgttgttt
     3961 gacggagctt atcaacgtag taagcgtgga acaaagaacg aagagagata aaaagttgat
     4021 ctttgtgaaa actacagaaa gtaaagaatg aaaagagtaa tgctaacata gcattacgga
     4081 ttttatgacc gatgatgaag aaaagaattt gaaacttagt ttatatgtgg taaaatgttt
     4141 taatcaagtt taggaggaat taattatgaa gtgtaattaa tgtaacaggg ttcaattaaa
     4201 agagggaagc gtatcattaa ccctataaac tacgtctgcc ctcattattg gagggtgaaa
     4261 tatgcccgtt acgattaaag acgtggccaa ggctgctggt gtttcgcctt caaccgtaac
     4321 ccgtgttatt caaaataaat caaccattag cgacgaaaca aaaaaacgcg ttcgcaaagc
     4381 tatgaaggaa ctcaactacc acccaaacct caacgctcgt agcttggtaa gcagctatac
     4441 tcaggttatc ggattagttc ttcctgatga ctcagacgcc ttctaccaga atcctttctt
     4501 tccatcggtt ctacgtggca tctctcaagt cgcatctgaa aaccactatg ccattcagat
     4561 agcaacaggg aaagatgaga aggagcgtct caacgctatt tcacaaatgg tctacggcaa
     4621 gcgtgtagat gggctaatct ttctctatgc ccaagaagaa gaccctctcg taaaactcgt
     4681 cgcagaagaa cagttcccct tccttatcct aggtaaatct ctatctcctt tcatcccact
     4741 tgtcgacaac gacaatgttc aagctggttt tgatgcgact gaatatttca tcaaaaaagg
     4801 ctgcaaacgc attgccttta tcggaggaag taaaaagctc ttcgtgacca aagaccgttt
     4861 aacaggctat gaacaggcgc ttaaacatta caaacttacc actgacaaca atcgcatcta
     4921 ctttgccgac gagtttctgg aagaaaaggg ctataaattt agcaagcgat tattcaagca
     4981 cgatccacaa attgatgcta tcatcacaac cgatagcctc ctagctgaag gtgtttgtaa
     5041 ctatattgcc aaacaccagc tggatgtccc tgttctcagc tttgactcgg ttaatcccaa
     5101 gctcaacttg gcagcctatg tcgatatcaa tagtttagag cttggtcgtg tttcccttga
     5161 aactattctc cagattatta atgataataa aaacaataaa caaatttgtt accgtcaatt
     5221 gatcgcccac aaaattatcg aaaaataatt ttcgagaccg gtctcacccc atcgttaagg
     5281 gatcaacttt gggagagagt tcaaaattga tccttttttt ataacagat
//
