LOCUS       V007934                 7790 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007934
VERSION     V007934
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Manso AS, Iannelli F, Pozzi G, Furi L, Oggioni MR.
  TITLE     The nucleotide sequence of the Streptococcus pneumoniae Escherichia
            coli shuttle vector pDP28
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7790)
  AUTHORS   Manso AS, Iannelli F, Pozzi G, Furi L, Oggioni MR.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2014) Department of Genetics, University of
            Leicester, Adrian Building, University Road, Leicester LE1 7RH, UK
REFERENCE   3  (bases 1 to 7790)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7790)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JAN-2014) Department of Genetics, University of Leicester,
            Adrian Building, University Road, Leicester LE1 7RH, UK"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..2392
                     /note="HindIII-SalI fragment of plasmid pSMB1 in INSDC
                     accession AF047385"
     rep_origin      184..201
                     /note="double strand replication origin of pneumococcal
                     plasmids pDP1 and pSMB1"
     CDS             403..1365
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label="rep"
                     /note="REP pDP1 pSMB1"
                     /protein_id="AHX11846.1"
                     /translation="MEKWENQDKILLDKNKRGKDRNWRGRKLLSLKLADIFKELGYRET
                     LIERVETCGDTLRFIRREDGSLRLYQAYFCKNKLCPMCNWRRSMKYSYQTSQIVDEAIK
                     EQPKGRFLFLTLTVKNVPGKELNATISQLTQSFDRLFRRAKVKKNLIGFLRSVEVTHNQ
                     EEETYHPHIHVLMMVKSSYFSGAGDNYVSQEEWGRMWEQSLKVDYVPMVDIRSVKEIGK
                     GLKGAILETAKYPIKPIKLDVENKQVVGDLYNGLYRKRQLGYGGLFKEIRKRLQLSNVE
                     NGDLVYTSDDNDEMSKGTKIVAIWNATKQNYFVKNKGWN"
     gene            403..1365
                     /gene="rep"
                     /label="rep"
     rep_origin      2352..2392
                     /note="single strand replication origin of pneumococcal
                     plasmids pDP1 and pSMB1"
     CDS             complement(4050..4706)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(4707..4809)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(5335..5880)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(6142..6876)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
ORIGIN
        1 aagctttttg gttctattca aatatggcta gataatctcc ctattccgat gaaaatactg
       61 ttaacaatag cattagtttt gtttttattc attgtatttc catatagagg aaaacggtaa
      121 aaacggtaat tagatatata aaaggagctg aaagctccgt aaaaaaccta ctcgaagagt
      181 aggttttttc ttatcttgat actagtagga aataactctc atgctatttt tagggtttcg
      241 acagggtcta aacccttgat actactgact ttttaggtaa aaaaatgccc ttgacttttt
      301 tcaacggatc ttttataatt tagttgttca gaaaaaatta taaaaagaga aaaaaggcaa
      361 aggtattttt gtatttcttt gtaattacag gtaaattata acatggagaa gtgggaaaat
      421 caagataaaa ttcttttaga taaaaataaa agaggcaagg atagaaactg gcgaggacga
      481 aaattattaa gtttgaaatt ggcagatatt tttaaagagt taggttatag ggaaaccttg
      541 atagaacgcg ttgagacttg tggagatact cttcgattta ttcgtcgaga agatgggagt
      601 ttgcgtttat atcaagccta tttttgtaag aataaattgt gtcctatgtg taattggaga
      661 cggtcaatga aatattctta tcaaacatct cagatagttg atgaagcaat taaagaacaa
      721 ccaaaagggc gttttctttt cttgactttg actgtgaaaa atgttccagg taaagaattg
      781 aacgcgacaa ttagccagct aactcagtcc tttgaccgtc tttttagacg ggcaaaggta
      841 aagaaaaatc taatcgggtt tttacgttcg gttgaggtga ctcataatca agaagaagag
      901 acctatcacc ctcatatcca tgttttgatg atggttaagt ctagctattt tagtggggct
      961 ggagataatt atgtatctca agaagaatgg gggcggatgt gggaacagtc gcttaaggtt
     1021 gactatgtgc caatggttga tattcggtca gttaaagaaa taggaaaagg tcttaaaggg
     1081 gcaattcttg aaacggcaaa atatccaatt aagccgatta agttagatgt tgaaaacaag
     1141 caagttgttg gtgatttgta taatgggtta tatcgtaaac ggcaattggg ttacggtgga
     1201 ttgtttaaag aaattcggaa gagattgcaa ctgtctaatg tcgaaaatgg agatctggtt
     1261 tatacttctg atgacaatga tgaaatgtca aaaggtacaa aaatagtcgc gatttggaat
     1321 gcgactaaac aaaactattt tgtaaaaaat aagggttgga attaaagtat caactctttt
     1381 attataacat aaaaaaagag taaaatagac ttgatttttt tgagtttaga tgtatattga
     1441 aggtgtgaaa attaaaaaga tagctatgtt tttttctggg tatacttgtt ggggtattta
     1501 ttgttctatt ttttagaaat tattttgttt tacttttgga atatattttt ggttaaaaag
     1561 caccctctaa aaagggtgct ttttgtgcgg ttattcgcac gaaagttctc tgcaagagtt
     1621 cctcggaaga gcgtttgtat tttgattgta cgtcaaaact ataaaaacat ttactctttg
     1681 tcaaagagta gcagaaaagc tagaataaaa tttttaattt tattctagct aatagtgcaa
     1741 gtaatagctt ttgtagcttg cctcttgtcc gttattcaga caagaggcag gagtttgtat
     1801 ttgttctaaa tgagtatttc atttagaaca aatacatcga tgatgaacat tgcttacaag
     1861 aatagagatt tgagtgatga agaatttaaa gaagaagctc atcagtttag agataaagag
     1921 ataaaaatac tcgaagaaaa attaaaggaa cttaatattt cttgtaaggt tgttggaata
     1981 aatagtataa aagatatgaa cgagtataaa gagattatgg aaaatgttaa tcaagcaaaa
     2041 gaggaattaa agagaataga tgaattaata gccgtcagga gctccagaat tgatttttta
     2101 gaggataaaa cccttagaat tacaggaaac caaagatgag cttaaaaacg aaatggagag
     2161 cctaaaaaga gatttatctt tttttagaaa cgaaaatact cgactgaaaa atgtagtttc
     2221 agattgtatc aaggtagtat ttttggggta tttttagtag aaagtatatt gtaatttctg
     2281 agcgatttat gccgtgaaag ctacttgacc gtaagctaga tcaaagtacg ctagtcaggg
     2341 acgagccgaa aggctgtagc gtttcggagc ggcacggaca aagtacgtcg accgatgccc
     2401 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     2461 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
     2521 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
     2581 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
     2641 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
     2701 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
     2761 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     2821 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg
     2881 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta
     2941 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     3001 acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg
     3061 gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat
     3121 ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct
     3181 ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt
     3241 gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct
     3301 gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa
     3361 gtctggaaac gcggaagtcc cctacgtgct gctgaagttg cccgcaacag agagtggaac
     3421 caaccggtga taccacgata ctatgactga gagtcaacgc catgagcggc ctcatttctt
     3481 attctgagtt acaacagtcc gcaccgctgt ccggtagctc cttccggtgg gcgcggggca
     3541 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     3601 cggcagcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     3661 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
     3721 cacctacatc tgtattaacg aagcgctaac cgtttttatc aggctctggg aggcagaata
     3781 aatgatcata tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc
     3841 atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata
     3901 gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc
     3961 gaatttctgc cattcatccg cttattatca cttattcagg cgtagcaacc aggcgtttaa
     4021 gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt
     4081 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     4141 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     4201 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     4261 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     4321 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     4381 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     4441 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga
     4501 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     4561 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     4621 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     4681 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     4741 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     4801 tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg
     4861 acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa
     4921 agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc
     4981 tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg
     5041 taacggcaaa agcaccgccg gacatcagcg ctagcggagt gtatactggc ttactatgtt
     5101 ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg
     5161 tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac
     5221 gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg
     5281 aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca
     5341 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
     5401 cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc
     5461 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     5521 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     5581 cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5641 aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc
     5701 ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct
     5761 ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc
     5821 tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa
     5881 gaagatcatc ttattaatca gataaaatat ttctagattt cagtgcaatt tatctcttca
     5941 aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg tttgacagct
     6001 tatcatcgat acaaattccc cgtaggcgct agggacctct ttagctcctt ggaagctgtc
     6061 agtagtatac ctaataattt atctacattc cctttagtaa cgtgtaactt tccaaattta
     6121 caaaagcgac tcatagaatt atttcctccc gttaaataat agataactat taaaaataga
     6181 caatacttgc tcataagtaa cggtacttaa attgtttact ttggcgtgtt tcattgcttg
     6241 atgaaactga tttttagtaa acagttgacg atattctcga ttgacccatt ttgaaacaaa
     6301 gtacgtatat agcttccaat atttatctgg aacatctgtg gtatggcggg taagttttat
     6361 taagacactg tttacttttg gtttaggatg aaagcattcc gctggcagct taagcaattg
     6421 ctgaatcgag acttgagtgt gcaagagcaa ccctagtgtt cggtgaatat ccaaggtacg
     6481 cttgtagaat ccttcttcaa caatcagata gatgtcagac gcatggcttt caaaaaccac
     6541 ttttttaata atttgtgtgc ttaaatggta aggaatactc ccaacaattt tatacctctg
     6601 tttgttaggg aattgaaact gtagaatatc ttggtgaatt aaagtgacac gagtattcag
     6661 ttttaatttt tctgacgata agttgaatag atgactgtct aattcaatag acgttacctg
     6721 tttacttatt ttagccagtt tcgtcgttaa atgcccttta cctgttccaa tttcgtaaac
     6781 ggtatcggtt tcttttaaat tcaattgttt tattatttgg ttgagtactt tttcactcgt
     6841 taaaaagttt tgagaatatt ttatattttt gttcatgtaa tcactccttc ttaattacaa
     6901 atttttagca tctaatttaa cttcaattcc tattatacaa aattttaaga tactgcacta
     6961 tcaacacact cttaagtttg cttctaagtc ttatttccat aacttctttt acgtttccgc
     7021 cattctttgc tgtttcgatt tttatgatat ggtgcaagtc agcacgaaca cgaaccgtct
     7081 tatctcccat tatatctttt tttgcactga ttggtgtatc atttcgtttt tctttttgtg
     7141 cgcctaaatt tcccacaatc actcacttct ttctatttct tcttattctt attttatcat
     7201 caacaatcac aaatcacttg tgatttgtga taagtgattt gtgattaata tataaaagcc
     7261 ctctttaaag ggcttttatg tttattttga gaaagatata aaatcaatat atcccttttc
     7321 tccgatttta caacggcatt gtaggacttt ctatctttcg ttttgattcc ttttaccagg
     7381 gtttcttttc cctctagtaa ttcttttaca tttgttttgg tgagtttttt ctttctaaaa
     7441 tgttcagcta aagtaaactt acattcagga taatttgaac aaccataaaa cgattttttt
     7501 aatacaatat tgttgccaca cttaggacat tttcctacaa tacttttttc tgcttctttt
     7561 tctttctgtt cctggtaatc agaaaaattt agtttttcta tatcgttagg tacagcttcc
     7621 agtaaatgaa caatgaattt tttgatattc gtaataaagt tctcttgatt gccttctctt
     7681 ttaccgattt tttttaaata cgtttcccat ttagccgtca tttcagcact cgttaaaagg
     7741 tgctgacttt caactgcctg gcacaataat tttccttttt cagttacaac
//
