LOCUS       40924_15110        5406 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDONRamp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Song J.
  TITLE     MYB26 controls Arabidopsis anther dehiscence by regulating secondary
            thickening in the endothecium
  JOURNAL   Thesis (2009) University of Nottingham, Sutton Bonington Campus, 
            Loughborough, UK, In press
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Song J.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2009) Plant and Crop Sciences, The School of 
            Biosciences, Sutton Bonington Campus, University of Nottingham, 
            Loughborough LE12 5RD, UK
REFERENCE   3  (bases 1 to 5406)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5406)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Thesis 
            (2009) University of Nottingham, Sutton Bonington Campus, 
            Loughborough, UK, In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2009) Plant and Crop Sciences, The School of Biosciences, 
            Sutton Bonington Campus, University of Nottingham, Loughborough LE12
            5RD, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On Sep 23, 2009 this sequence version replaced FN398073.1.
FEATURES             Location/Qualifiers
     source          1..5406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    374..605
                     /label=attP1
                     /note="recombination site for the Gateway(R) BP reaction 
                     (pDONR(TM)221 version)"
     CDS             complement(1004..1306)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(1654..2310)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(2311..2413)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(2558..2789)
                     /label=attP2
                     /note="recombination site for the Gateway(R) BP reaction 
                     (pDONR(TM)221 version)"
     protein_bind    complement(3214..3230)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3238..3268)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(3283..3304)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3595..4183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4357..5214)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5215..5319)
                     /label=AmpR promoter
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tccaaataat gattttattt tgactgatag tgacctgttc gttgcaacac
      421 attgatgagc aatgcttttt tataatgcca actttgtaca aaaaagctga acgagaaacg
      481 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      541 tactgtaaaa cacaacatat ccagtcacta tgaatcaact acttagatgg tattagtgac
      601 ctgtagtcga ccgacagcct tccaaatgtt cttcgggtga tgctgccaac ttagtcgacc
      661 gacagccttc caaatgttct tctcaaacgg aatcgtcgta tccagcctac tcgctattgt
      721 cctcaatgcc gtattaaatc ataaaaagaa ataagaaaaa gaggtgcgag cctctttttt
      781 gtgtgacaaa ataaaaacat ctacctattc atatacgcta gtgtcatagt cctgaaaatc
      841 atctgcatca agaacaattt cacaactctt atacttttct cttacaagtc gttcggcttc
      901 atctggattt tcagcctcta tacttactaa acgtgataaa gtttctgtaa tttctactgt
      961 atcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca
     1021 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga
     1081 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga
     1141 tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt gcactggcca
     1201 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca
     1261 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg
     1321 ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc
     1381 tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg
     1441 cttaccagac cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt
     1501 caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata
     1561 catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct
     1621 ggcttttagt aagccggatc cacgcggcgt ttacgccccg ccctgccact catcgcagta
     1681 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac
     1741 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     1801 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     1861 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     1921 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     1981 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     2041 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg
     2101 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     2161 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     2221 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     2281 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     2341 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     2401 tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac
     2461 agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc
     2521 gcaaagtgcg tcgggtgatg ctgccaactt agtcgactac aggtcactaa taccatctaa
     2581 gtagttgatt catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2641 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2701 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2761 cactatcagt caaaataaaa tcattatttg ccatccagct gatatctttc tagaagatct
     2821 cctacaatat tctcagctgc catggaaaat cgatgttctt cttttattct ctcaagattt
     2881 tcaggctgta tattaaaact tatattaaga actatgctaa ccacctcatc aggaaccgtt
     2941 gtaggtggcg tgggttttct tggcaatcga ctctcatgaa aactacgagc taaatattca
     3001 atatgttcct cttgaccaac tttattctgc attttttttg aacgaggttt agagcaagct
     3061 tcaggaaact gagacaggaa ttttattaaa aatttaaatt ttgaagaaag ttcagggtta
     3121 atagcatcca ttttttgctt tgcaagttcc tcagcattct taacaaaaga cgtctctttt
     3181 gacatgttta aagtttaaac ctcctgtgtg aaattgttat ccgctcacaa ttccacacat
     3241 tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     3301 attaattgcg ttgcgctcac tgccaattgc tttccagtcg ggaaacctgt cgtgccagct
     3361 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3421 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3481 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3541 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3601 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3661 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3721 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3781 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3841 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3901 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3961 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4021 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4081 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4141 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4201 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4261 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4321 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4381 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4441 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4501 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4561 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4621 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4681 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4741 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4801 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4861 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4921 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     4981 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     5041 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     5101 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     5161 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     5221 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5281 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5341 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     5401 gaggcc
//
