LOCUS       Exported                4777 bp DNA     circular SYN 03-SEP-2024
DEFINITION  Gateway(R) donor vector with attP4 and attP1 (reversed) sites and a 
            kanamycin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4777)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4777)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..824
                     /label=attP4
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1184..1486)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(1831..2487)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(2488..2590)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    2748..2979
                     /label=attP1
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3019..3037)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3042..3058)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3171..3977
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      4127..4715
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgaa cgagaaacgt aaaatgatat aaatatcaat
      721 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatg
      781 cagtcactat gaatcaacta cttagatggt attagtgacc tgtagaattc gagctcgacc
      841 gacagccttc caaatgttct tctcaaacgg aatcgtcgta tccagcctac tcgctattgt
      901 cctcaatgcc gtattaaatc ataaaaagaa ataagaaaaa gaggtgcgag cctctttttt
      961 gtgtgacaaa ataaaaacat ctacctattc atatacgcta gtgtcatagt cctgaaaatc
     1021 atctgcatca agaacaattt cacaactctt atacttttct cttacaagtc gttcggcttc
     1081 atctggattt tcagcctcta tacttactaa acgtgataaa gtttctgtaa tttctactgt
     1141 atcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca
     1201 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga
     1261 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga
     1321 tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt gcactggcca
     1381 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca
     1441 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagtccctg
     1501 ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc
     1561 tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg
     1621 cttaccagac cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt
     1681 caactgtcac tgtaatacgc tgcttcatag cacacctctt tttgacatac ttcgggtata
     1741 catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct
     1801 ggcttttagt aagccggatc cacgcgatta cgccccgccc tgccactcat cgcagtactg
     1861 ttgtaattca ttaagcattc tgccgacatg gaagccatca cagacggcat gatgaacctg
     1921 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     1981 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     2041 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     2101 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     2161 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     2221 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga attccggatg
     2281 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     2341 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     2401 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     2461 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     2521 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     2581 gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg
     2641 gacaccagga tttatttatt ctgcgaagtg atcttccgtc acaggtattt attcggcgca
     2701 aagtgcgtcg ggtgatgctg ccaacttagt cgacgctcta gagctccaaa taatgatttt
     2761 attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct tttttataat
     2821 gccaactttg tacaaaaaag ttgaacgaga aacgtaaaat gatataaata tcaatatatt
     2881 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatgcagtc
     2941 actatgaatc aactacttag atggtattag tgacctgtag aattcgagct ctagagctgc
     3001 agggcggccg cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     3061 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     3121 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     3181 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     3241 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga
     3301 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     3361 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     3421 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag
     3481 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     3541 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     3601 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     3661 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
     3721 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     3781 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     3841 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     3901 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     3961 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
     4021 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt
     4081 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4141 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4201 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4261 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4321 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4381 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4441 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4501 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4561 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4621 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4681 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4741 ggttcctggc cttttgctgg ccttttgctc acatgtt
//
