LOCUS       Exported                5587 bp DNA     circular SYN 03-SEP-2024
DEFINITION  Gateway(R) donor vector with attP1 and attP2 sites and a gentamycin 
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5587)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5587)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(192..278)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    332..563
                     /label=attP1
                     /note="recombination site for the Gateway(R) BP reaction 
                     (pDONR(TM)201 version)"
     CDS             complement(962..1264)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(1612..2267)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG"
     promoter        complement(2268..2370)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(2515..2746)
                     /label=attP2
                     /note="recombination site for the Gateway(R) BP reaction 
                     (pDONR(TM)201 version)"
     CDS             complement(3533..4063)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(4252..4280)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      4937..5525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa
      421 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
      481 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg aatcaactac
      541 ttagatggta ttagtgacct gtagtcgacc gacagccttc caaatgttct tcgggtgatg
      601 ctgccaactt agtcgaccga cagccttcca aatgttcttc tcaaacggaa tcgtcgtatc
      661 cagcctactc gctattgtcc tcaatgccgt attaaatcat aaaaagaaat aagaaaaaga
      721 ggtgcgagcc tcttttttgt gtgacaaaat aaaaacatct acctattcat atacgctagt
      781 gtcatagtcc tgaaaatcat ctgcatcaag aacaatttca caactcttat acttttctct
      841 tacaagtcgt tcggcttcat ctggattttc agcctctata cttactaaac gtgataaagt
      901 ttctgtaatt tctactgtat cgacctgcag actggctgtg tataagggag cctgacattt
      961 atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat
     1021 cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc
     1081 gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggagact ttatctgaca
     1141 gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca ataatatcac
     1201 tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact
     1261 gcatttcacc agcccctgtt ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg
     1321 acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacgcag acgacgggct
     1381 tcattctgca tggttgtgct taccagaccg gagatattga catcatatat gccttgagca
     1441 actgatagct gtcgctgtca actgtcactg taatacgctg cttcatagca tacctctttt
     1501 tgacatactt cgggtataca tatcagtata tattcttata ccgcaaaaat cagcgcgcaa
     1561 atacgcatac tgttatctgg cttttagtaa gccggatcca cgcggcgttt acgccccccc
     1621 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     1681 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     1741 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     1801 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
     1861 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     1921 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     1981 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     2041 gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     2101 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     2161 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     2221 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     2281 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     2341 tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccaaaa gttggcccag
     2401 ggcttcccgg tatcaacagg gacaccagga tttatttatt ctgcgaagtg atcttccgtc
     2461 acaggtattt attcggcgca aagtgcgtcg ggtgatgctg ccaacttagt cgactacagg
     2521 tcactaatac catctaagta gttgattcat agtgactgga tatgttgtgt tttacagtat
     2581 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
     2641 gtttctcgtt cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     2701 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgca
     2761 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2821 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     2881 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     2941 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     3001 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     3061 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     3121 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     3181 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     3241 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     3301 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     3361 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     3421 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     3481 tgacgagggg aaattaggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg
     3541 tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag
     3601 ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt
     3661 tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc
     3721 cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa
     3781 cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg
     3841 tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg
     3901 ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt
     3961 cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag
     4021 ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct gctccataac
     4081 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt
     4141 acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
     4201 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga
     4261 accgaacagg cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac
     4321 ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc
     4381 aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag
     4441 gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc
     4501 ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag
     4561 catcgtttgt tcgcccagga ctctagctat agttctagtg gttggctact aataggttgt
     4621 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     4681 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     4741 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     4801 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     4861 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5581 acatgtt
//
