LOCUS       40924_15015        8596 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pDOJHR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Lee JH, O'sullivan DJ.
  TITLE     Sequence Analysis of Two Cryptic Plasmids from Bifidobacterium 
            longum DJO10A and Construction of a Shuttle Cloning Vector
  JOURNAL   Appl. Environ. Microbiol. 72 (1), 527-535 (2006)
  PUBMED    16391088
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Lee J-H., O'Sullivan DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2005) Food Science and Nutrition, University of 
            Minnesota, 1500 Gortner Ave, St. Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 8596)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8596)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2006"; volume: "72"; issue: "1"; pages:
            "527-535"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-JUL-2005) Food Science and Nutrition, University of Minnesota, 
            1500 Gortner Ave, St. Paul, MN 55108, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1870..2328)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="chloramphenicol resistance"
                     /protein_id="AAZ32757.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPEKCFFSFYYSMDFIYWV"
     gene            complement(1870..2328)
                     /gene="cat"
                     /label=cat
     CDS             2791..2802
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     promoter        complement(4082..4186)
                     /label=AmpR promoter
     primer_bind     4660..4676
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(4680..4736)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(4746..4762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4770..4786)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4794..4824)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4839..4860)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        complement(5313..5341)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     rep_origin      5453..5998
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(6200..7678)
                     /codon_start=1
                     /gene="mob"
                     /product="mobilization protein"
                     /label=mob
                     /protein_id="AAZ32759.1"
                     /translation="MAIYSLNISSASSAVAALSYITSMRVRDDARGETYSGFGRRERVA
                     HVATLLPEGAPSGYADPERLFNAAQAAERGTGVAAKKIMVALPRELDEGQRVLAVERFI
                     RENLTAGGYAATYAIHLDREGRNPHAHILVANRRIDPRTGEWARLKQKTTFALDGNGQR
                     IPVIDPKTGVQKVDKRNRKQWKRVTVSENPLGTKAMLLSMRESWADVCNGLLPEGVRID
                     HRSLEEQGIDRVPTIHEGYASREMEKRGQPSDRMAINREIGASNRDLAEGDAAVDDLRA
                     HQSELRRLLRDMADRAVQAFDRMLESIGHGVDPSDAVEAMRDRDGINLMIPRNAKDSDR
                     PVWHAWLYDRHEWAAIPSHVLREGMRTVGRVRAAIGTALHRWRDAQAARRERIARSTPA
                     QAPRLADVARQAREDAGQPAHQPRPVRKPEQQPEQQRERPVSLAAAAKEASKQLKREQQ
                     QEPEPEPALEWNPWDPADPMNLGMGGSQGYGLGL"
     gene            complement(6200..7678)
                     /gene="mob"
                     /label=mob
     CDS             8011..8493
                     /codon_start=1
                     /gene="orfIV"
                     /product="hypothetical protein"
                     /label=orfIV
                     /protein_id="AAZ32760.1"
                     /translation="MIEPIGGNEMPKSFAQQIEDDENKIKRIREHQRMVRAKQAKQERN
                     ARTKRLVETGAIVEKAHGGAYDDEGRQTFSDGLNGIISVYDPSRGGNVDMRVIDVIDRR
                     IPRLPRSETTTGTAAAASRTVQATAPQPAHAQPQSFTPNPQRIEHRTGAQQPDRWA"
     gene            8011..8493
                     /gene="orfIV"
                     /label=orfIV
ORIGIN
        1 actgaacttc gtgagcagtg ggagcagctt tacctgccgc tgcgcccgct gtgcacgaac
       61 gacttcatcg agggcgtgta ccggcagccc agggcgaagg cgctggaggg ctaccggtac
      121 atcgaggcga accccaagac cgtcagcgac ctgctcgtgg tcgacatcga cgacgcgaac
      181 gcccgcgcga tggccctatg ggagcatgag gggatgctgc ccaacgtgat cgtggagaac
      241 ccgagaaacg gccacgcgca cgccgtgtgg gcgctggccg ccccgttcag tcgcaccgaa
      301 tacgcgcggc gcaagccgct cgccctggcc gcggcggtca cggaggggct gcgccgctcg
      361 tgcgacgggg acaagggata ttcggggctg atgaccaaga acccgctgca cgaggcgtgg
      421 aacagcgaac tggtcaccga tcacctgtac gggctggacg aactgcgcga ggcgctggag
      481 gagtccgggg acatgccgcc ggcctcgtgg aagcgcacga aacggcgcaa cgtggtcgga
      541 ttgggccgca actgccatct gttcgagacg gcgcgcacat gggcgtacag ggaggtgcgc
      601 caccactggg gagacagcga ggggctgcgg ctggccatct cggccgaggc gcacgaaatg
      661 aacgccgaac tgttccccga gccactggca tggtcggagg tcgagcagat cgccaagagc
      721 atccacaagt ggatcgtcac ccaaagccgc atgtggcgcg acggccccac cgtctacgag
      781 gcgacattca tcaccgtcca aagcgcccga gggaaaaagt caggcaaggc cagacgagaa
      841 acattcgaac tattcgcgag cgaggagatg acgtaatggt tattcaaacg attcgcaaga
      901 agcgtcccct gcccgcgcgg caactcgctg aaatgtatga cgttactcca cgcaccatca
      961 tgagatgggc agcacagacc cgtgctgatt ggattgatga acaggcggct ggacgcgagg
     1021 cgatccgcgc atatcacgat gatgatggac attcatggac tcaaaccgcc aagcatttcc
     1081 acctttctct gtccacggtg aaagaacgcg cataccgtgc ccgtaaggag cgtgccgccg
     1141 aggccgagga aaaagccaga aatgaggtcc acaagaacga ggtgccgctt ttcgactagg
     1201 ctggcgcttg accccggtgc ctcccgtgca attggagcgc ggcaagggcc agggtctttc
     1261 acgtctaggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca
     1321 tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag
     1381 gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct
     1441 ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat
     1501 ggggaaggcc atccagcctc gcgtcatgtg aggcattttc gctctttccg gcgaggctag
     1561 ttacccttaa gttattggta tgactggttt taagcgcaaa aaaagttgct ttttcgtacc
     1621 tattaatgta tcgttagaaa accgactgta aaaagtacag tcggcattat ctcatattat
     1681 aaaagccagt cattaggcct atctgacaat tcctgaatag agttcataaa caatcctgca
     1741 tgataaccat cacaaacaga atgatgtacc tgtaaagata gcggtaaata tattgaatta
     1801 cctttattaa tgaattttcc tgctgtaata atgggtagaa ggtaattact attattattg
     1861 atatttaagt taaacccagt aaatgaagtc catggaataa tagaaagaga aaaagcattt
     1921 ttcaggtata ggtgttttgg gaaacaattt ccccgaacca ttatatttct ctacatcaga
     1981 aaggtataaa tcataaaact ctttgaagtc attctttaca ggagtccaaa taccagagaa
     2041 tgttttagat acaccatcaa aaattgtata aagtggctct aacttatccc aataacctaa
     2101 ctctccgtcg ctattgtaac cagttctaaa agctgtattt gagtttatca cccttgtcac
     2161 taagaaaata aatgcagggt aaaatttata tccttcttgt tttatgtttc ggtataaaac
     2221 actaatatca atttctgtgg ttatactaaa agtcgtttgt tggttcaaat aatgattaaa
     2281 tatctctttt ctcttccaat tgtctaaatc aattttatta aagttcattt gatatgcctc
     2341 ctaaattttt atcttgctct tttgtcagag aaatcataac tctttttttc gattctgaaa
     2401 tcaccattta aaaaactcca atcaaataat tttataaaat tagtgtatca ctttgtaatc
     2461 ataaaaccaa caataaagct acttaaatat agatttataa aaaacgttgg cgaaaacgtt
     2521 ggcgattcgt tggcgattga aaaacccctc aaacccttga gccagttggg atagagcgtt
     2581 tttggcacaa aaattggcac tcggcactta atggggggtc gtagtacgga agcaaaattc
     2641 gcttcctttc cccccatttt tttccaaatt ccaaattttt ttcaaaaatt ttccagcgct
     2701 accgctcggc aaaattgcaa gcaattttta aaatcaaacc catgagggaa tttcattccc
     2761 tcaaactccc ttgagcctcc tccaaccgaa atagaaggac gctgcgctta ttatttcatt
     2821 cagtcatcgg ctttcataat ctaacagaca acatcttcgc tgcaaagcca cgctacgctc
     2881 aagggctttt acgctacgat aacgcctgtt ttaacgatta tgccgataac taaacgaaat
     2941 aaacgctaaa acgtctcaga aacgattttg agacgtttta ataaaaaatc gacttcgttc
     3001 tttttttacc tctcggttat gagttagttc aaattcgttc tttttaggtt ctaaatcgtg
     3061 tttttcttgg aattgtgctg ttttatcctt taccttgtct acaaacccct taaaaacgtt
     3121 tttaaaggct tttaagccgt ctgtacgttc cttaaggaat tcgagctcgg taccccgaac
     3181 gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg
     3241 ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg
     3301 aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa
     3361 atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata
     3421 agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
     3481 ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
     3541 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
     3601 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
     3661 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     3721 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcactcaa
     3781 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3841 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3901 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3961 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4021 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4081 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4141 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4201 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     4261 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     4321 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     4381 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     4441 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     4501 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     4561 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     4621 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
     4681 agcttgcatg cctgcaggtc gactctagag gatccccggg taccgagctc gaattcgtaa
     4741 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     4801 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     4861 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     4921 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgatccacag
     4981 gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag
     5041 gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg
     5101 catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac
     5161 gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag
     5221 ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact
     5281 gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca
     5341 aacatgagaa ttacaactta tatcgtatgg ggctgacttc aggtgctaca tttgaagaga
     5401 taaattgcac tgaaatctag aaatatttta tctgattaat aagatgatct tcttgagatc
     5461 gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt
     5521 tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt ggaggagcgc
     5581 agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc aagactaact
     5641 cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg
     5701 gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg gggttcgtgc
     5761 atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga
     5821 caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg
     5881 cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5941 ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa
     6001 cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa
     6061 tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag
     6121 tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg caccggtgca
     6181 gccttttttc tcctgccacc tagaggccga ggccgtagcc ttggctgccg cccatgccga
     6241 ggttcatggg gtcggccggg tcccacgggt tccattcgag tgccggctct ggttccggct
     6301 cctgctgttg ctcccgtttg agctgtttgc tggcctcctt ggccgctgcg gcgaggctca
     6361 cgggccgctc ccgctgctgc tcgggctgtt gctcgggctt cctgacggga cggggctggt
     6421 gtgccggttg tcccgcgtcc tcgcgcgcct gacgggccac gtcagccaag cggggcgcct
     6481 gggcgggcgt ggaacgggcg atccgctcgc gtctggcggc ctgcgcgtcc cgccaacgat
     6541 gcagggccgt gccgatggcg gcgcggacgc ggcccacagt gcgcattccc tcccgcagga
     6601 cgtgggaggg aatggccgcc cactcgtgcc ggtcgtatag ccaggcatgc cagacgggac
     6661 ggtcggaatc cttggcgttc cggggaatca tgaggttgat gccgtcccgg tcccgcatcg
     6721 cctccaccgc gtcggacggg tccacgccgt gtccgatgct ctccagcatc cggtcgaacg
     6781 cttggacggc gcggtcggcc atgtcgcgca ggagccggcg gagttcggac tggtgcgcgc
     6841 gcaggtcgtc cacggcagcg tcgccttcgg ccaggtcgcg gttcgaggct ccgatctcgc
     6901 ggttgatggc catgcggtcg gacggctggc ctcgtttctc catctcccgg ctggcgtagc
     6961 cctcgtggat ggtgggcacg cggtcgatgc cctgttcctc aaggctgcgg tggtcgatgc
     7021 gcacgccctc gggcagcagc ccgttgcaca cgtcggccca gctctcgcgc atgctcagca
     7081 gcatggcctt ggtgcccaaa gggttctcgc tgacggtgac gcgcttccac tgcttgcggt
     7141 tgcgcttgtc caccttctgc acgccggtct tcgggtcgat gaccgggatg cgctggccgt
     7201 tcccgtccag cgcgaacgtg gtcttctgct tgagccgcgc ccattccccg gtccgagggt
     7261 cgatcctgcg gttggcgacg aggatgtgcg cgtgcgggtt cctcccctcg cggtccaaat
     7321 ggatcgcgta ggtcgccgcg tacccgccgg cggtcaggtt ctcacggatg aaccgttcca
     7381 ccgccaggac gcgctgcccc tcgtccagct cgcgcggcag ggccaccatg atcttcttcg
     7441 cggccacgcc cgtgccgcgc tccgccgcct gggcggcgtt gaacaggcgc tccggatccg
     7501 cgtagccgct gggcgcgccc tcgggcagga gcgtcgccac atgggccacg cgctccctgc
     7561 gcccgaaccc ggaataggtc tcgcccctcg cgtcgtcccg cacgcgcatc gacgtgatgt
     7621 acgacaacgc ggccacggcg ctggacgcgc tggagatgtt caacgagtag atcgccacgg
     7681 cgacctcctt ccacgcgtgc gggcacgggg attctcaagg ggccggcccg aggccccttg
     7741 agcccgccgg gaggcgcccc cggcagggcg ggaatccaaa gggcggagcc ctgtggccct
     7801 ccccgggcag gggcgggatc gtcaagggcg gagcccttgg ccccctcggg agagcgcact
     7861 gacacaatgc tacctccggt agcattaagt gcgccctccg ccatgcggag ggacgggccg
     7921 cgaccggata tgcggggaac gtccacgacg cgtcttccgt gtcctccgtc ctgccttgtg
     7981 ccgtcgatta taatcctcgg ataacggacg atgattgaac cgattggagg aaacgagatg
     8041 ccgaagagct tcgcgcagca gatcgaggac gacgagaaca agatcaagcg catccgggag
     8101 caccagcgca tggtcagggc caaacaagcc aaacaggagc gcaacgcccg caccaaaagg
     8161 ctcgtggaga ccggagcgat agtggaaaaa gcgcacggcg gcgcgtacga cgacgaagga
     8221 cggcagacgt tctcggatgg tctgaacggc atcatctcgg tctacgaccc gtcccgcggc
     8281 ggcaacgtgg acatgagagt catcgacgta atcgaccggc gcatccccag attgccaagg
     8341 tccgaaacca caacaggcac ggcagcggcg gcatcgcgaa ccgtgcaagc caccgcacca
     8401 cagccagccc acgcccaacc gcaaagcttc acgcccaacc cccagcgcat cgaacaccgg
     8461 acaggagcac agcagccgga tcggtgggcc tagccgggac ggcgcagccc tcgctccgct
     8521 gggctgaaat atctgacttg gttcgtaaca tatttccgac atgtcggaaa tatgtcgtat
     8581 catcgttgct atgagt
//
