LOCUS       40924_15010       13317 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle cloning vector pDOJ4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13317)
  AUTHORS   Lee JH, Halgerson JS, Kim JH, O'Sullivan DJ.
  TITLE     Comparative sequence analysis of plasmids from Lactobacillus 
            delbrueckii and construction of a shuttle cloning vector
  JOURNAL   Appl. Environ. Microbiol. 73 (14), 4417-4424 (2007)
  PUBMED    17526779
REFERENCE   2  (bases 1 to 13317)
  AUTHORS   Lee J-H., Halgerson JS, Kim J-H., O'Sullivan DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2006) Center for Microbial and Plant Genomics, 
            University of Minnesota, 1500 Gortner Ave, St. Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 13317)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13317)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2007"; volume: "73"; issue: "14"; 
            pages: "4417-4424"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-DEC-2006) Center for Microbial and Plant Genomics, University of
            Minnesota, 1500 Gortner Ave, St. Paul, MN 55108, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        1505..1533
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     protein_bind    1986..2007
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2022..2052
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2060..2076
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2084..2100
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2110..2166
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2170..2186)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2660..2764
                     /label=AmpR promoter
     CDS             complement(4044..4055)
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     CDS             4518..4976
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="derived from pCI372 for chloramphenicol resistance"
                     /protein_id="ABM79767.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPEKCFFSFYYSMDFIYWV"
     gene            4518..4976
                     /gene="cat"
                     /label=cat
     CDS             7073..7651
                     /codon_start=1
                     /product="putative mobilization protein"
                     /label=putative mobilization protein
                     /note="ORF1; derived from Lactobacillus delbrueckii subsp. 
                     bulgaricus plasmid pDOJ1"
                     /protein_id="ABM79768.1"
                     /translation="MENSAGMLSVGQIAKMLKTNRQNIYNVLKTEHIKADGYNDKHYSL
                     YSPETIQEIKAALSKKATLRSKKVVAKEQAEEIADLKNQLSEQQRLTTWLQSQLVQLQV
                     EADKLRSQNSQLQLDHAKTQLLIGQVDQEKTTLKAENDRLSAENDKLGQLTDKVLKDAQ
                     RAEEDAQKAKADLDKARRAGLWSRITRNY"
     CDS             7952..8473
                     /codon_start=1
                     /product="putative transposase"
                     /label=putative transposase
                     /note="ORF2; derived from Lactobacillus delbrueckii subsp. 
                     bulgaricus plasmid pDOJ1"
                     /protein_id="ABM79769.1"
                     /translation="MTYKEWTDSDHLELVKNWKLHGLTNVEIAQRIGIAEKTLYVWLKK
                     SPKLKKAIRGGKNIARARAENALYELALNGDRQALFFWLKNNYRERYSDKPLSPAEADL
                     MSQKARLAKLQADLAEAQLKAIKEDQGDQATQLNNLLDSLKEAVLDEGISPDNIVPTGN
                     GLIIDDISDS"
     CDS             9301..9312
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     CDS             9322..9549
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="ORF4; derived from Lactobacillus delbrueckii subsp. 
                     bulgaricus plasmid pDOJ1"
                     /protein_id="ABM79771.1"
                     /translation="MMSLEEREREIEKVVRIAEADFNNAYHLNAINKEDVIKNRAYKYA
                     EVLKLQELLALNKTIKDGLNGIEMSVDLIE"
     CDS             9564..10535
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="ORF5; derived from Lactobacillus delbrueckii subsp. 
                     bulgaricus plasmid pDOJ1"
                     /protein_id="ABM79772.1"
                     /translation="MNNSEKTSLMAEPYNSNRNAIDRLRVNQKALQAGSVKREENYIAD
                     ESGNSEGFRMAFYTAYKSGVQLAIPVEAEPGKMVINETFSKVQHLLIASWYSQADRASN
                     FRIQLTFKEISEALGYSRSQATALRKQLRDLITPLVRCTFVTSNKDSIDAVNLFAAGSY
                     SKGQLTMWLTPNMAETFLSEESSTEYFPLSLLKLKGTAYYLALKIMHNANINARWRADR
                     VDRLGLEDTLKALPTLPDPVENAQGTSRHLQRQIIAPLAKAIEELEAVTGIVVRPSQPL
                     KGLKAKDLSKVTLNVTDWGQVDIAELTRKKRKRLRKNNVRED"
     rep_origin      10558..10622
                     /label=derived from pDOJ1
                     /note="derived from pDOJ1"
     repeat_region   10558..10578
                     /label=direct repeat I for iteron structure
                     /note="direct repeat I for iteron structure"
     repeat_region   10580..10600
                     /label=direct repeat II for iteron structure
                     /note="direct repeat II for iteron structure"
     misc_feature    10602..10622
                     /label=direct repeat III for iteron structure
                     /note="direct repeat III for iteron structure"
     CDS             10830..12896
                     /codon_start=1
                     /product="putative primase-helicase"
                     /label=putative primase-helicase
                     /note="ORF6; derived from Lactobacillus delbrueckii subsp. 
                     bulgaricus plasmid pDOJ1"
                     /protein_id="ABM79773.1"
                     /translation="MNNLVKPADLKSLISLPEYIASVVSMDSKGFFSCVNPNHPDKHPS
                     MCVDQNHPQYVHCFSCQASYDLFDCWALINDGVTETRKNSAGKEKPVYNFNAVAIEIAD
                     HYGYTLIGDPANNLPEPPLPEPEPEPAQTQTSNDSNIRAQLEAWHASLNQTDYLQKRGI
                     TQTTAEIFNLGYSALTNSIIIPYGQDGYYVQRAINPIEKHDRYRFPIGQVRVYNAEALK
                     ECKTVFIVEGQFDALSIMQESGVGAVATSTSQTRLIVKTLQKFKEQDPTINPTIILSMD
                     NDRAGQKANRALQRDLEAHGFTCYVNPVNGDYKDANEFLVKDREGFRQKLQHVINQPDN
                     WLDKYYADIKNRHDYPDNIPTGFKNLDDELDGGLQPKLYVLGAVSSLGKTTFALNIADN
                     LAKQGRHVFFFSMESSKREVTDKLLSRASCLSNGHKWTQLQVSRGAWLNNAEDKEEFDG
                     LFKAFSRYQRFLHIYDNRVKASQVKDLVNSWLGNHPDEKKPLVVVDYLQILQAEQDNVT
                     DKAKVTDSVSVLSELTKQAEVPVLVISSLNRASYWQDVSFESFKESGEIEYSADVMLGL
                     EFAHREEYITVQKNGHVELNKEKFDQRKQEVPRRVEMVILKNRTGKTGGHIFFKYNAMF
                     NSYQACTEKEAAIANNFKMLYHTKQVGKPVEAARIEYTVDPETGRVTEKNQDK"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
     1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
     1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
     1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
     1861 ccgtcctgtg gatcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     1921 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     1981 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     2041 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     2101 catgattacg aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
     2161 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     2221 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     2281 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     2341 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     2401 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     2461 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     2521 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     2581 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     2641 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2701 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2761 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2821 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2881 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2941 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3001 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3061 cttggttgag tgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     3121 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     3181 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     3241 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     3301 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc
     3361 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     3421 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
     3481 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
     3541 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
     3601 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
     3661 ctggcgttcg gggtaccgag ctcgaattcc ttaaggaacg tacagacggc ttaaaagcct
     3721 ttaaaaacgt ttttaagggg tttgtagaca aggtaaagga taaaacagca caattccaag
     3781 aaaaacacga tttagaacct aaaaagaacg aatttgaact aactcataac cgagaggtaa
     3841 aaaaagaacg aagtcgattt tttattaaaa cgtctcaaaa tcgtttctga gacgttttag
     3901 cgtttatttc gtttagttat cggcataatc gttaaaacag gcgttatcgt agcgtaaaag
     3961 cccttgagcg tagcgtggct ttgcagcgaa gatgttgtct gttagattat gaaagccgat
     4021 gactgaatga aataataagc gcagcgtcct tctatttcgg ttggaggagg ctcaagggag
     4081 tttgagggaa tgaaattccc tcatgggttt gattttaaaa attgcttgca attttgccga
     4141 gcggtagcgc tggaaaattt ttgaaaaaaa tttggaattt ggaaaaaaat ggggggaaag
     4201 gaagcgaatt ttgcttccgt actacgaccc cccattaagt gccgagtgcc aatttttgtg
     4261 ccaaaaacgc tctatcccaa ctggctcaag ggtttgaggg gtttttcaat cgccaacgaa
     4321 tcgccaacgt tttcgccaac gttttttata aatctatatt taagtagctt tattgttggt
     4381 tttatgatta caaagtgata cactaatttt ataaaattat ttgattggag ttttttaaat
     4441 ggtgatttca gaatcgaaaa aaagagttat gatttctctg acaaaagagc aagataaaaa
     4501 tttaggaggc atatcaaatg aactttaata aaattgattt agacaattgg aagagaaaag
     4561 agatatttaa tcattatttg aaccaacaaa cgacttttag tataaccaca gaaattgata
     4621 ttagtgtttt ataccgaaac ataaaacaag aaggatataa attttaccct gcatttattt
     4681 tcttagtgac aagggtgata aactcaaata cagcttttag aactggttac aatagcgacg
     4741 gagagttagg ttattgggat aagttagagc cactttatac aatttttgat ggtgtatcta
     4801 aaacattctc tggtatttgg actcctgtaa agaatgactt caaagagttt tatgatttat
     4861 acctttctga tgtagagaaa tataatggtt cggggaaatt gtttcccaaa acacctatac
     4921 ctgaaaaatg ctttttctct ttctattatt ccatggactt catttactgg gtttaactta
     4981 aatatcaata ataatagtaa ttaccttcta cccattatta cagcaggaaa attcattaat
     5041 aaaggtaatt caatatattt accgctatct ttacaggtac atcattctgt ttgtgatggt
     5101 tatcatgcag gattgtttat gaactctatt caggaattgt cagataggcc taatgactgg
     5161 cttttataat atgagataat gccgactgta ctttttacag tcggttttct aacgatacat
     5221 taataggtac gaaaaagcaa ctttttttgc gcttaaaacc agtcatacca ataacttaag
     5281 ggtaactagc ctcgccggaa agagcgaaaa tgcctcacat gacgcgaggc tggatggcct
     5341 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
     5401 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta
     5461 ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga
     5521 gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg
     5581 cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct
     5641 cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa
     5701 tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac
     5761 gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc
     5821 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat
     5881 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg
     5941 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtccc ctacgtgctg
     6001 ctgaagttgc ccgcaacaga gagtggaacc aaccggtgat accacgatac tatgactgag
     6061 agtcaacgcc atgagcggcc tcatttctta ttctgagtta caacagtccg caccgctgtc
     6121 cggtagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     6181 ctttatcatg caactcgtag gacaggtgcc ggcagcgccc aacagtcccc cggccacggg
     6241 gcctgccacc atacccacgc cgaaacaagc gccctgcacc attatgttcc ggatctgcat
     6301 cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga agcgctaacc
     6361 gtttttatca ggctctggga ggcagaataa atgatcatat cgtcaattat tacctccacg
     6421 gggagagcct gagcaaactg gcctcaggca tttgagaagc acacggtcac actgcttccg
     6481 gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct
     6541 gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac
     6601 ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc
     6661 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     6721 gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     6781 cgtataatat ttgcccatgg cttaaatttg gcatacagac agcaagggga gcgggcaacg
     6841 gggaagagca gacatttaat tcgtttttgg gcgtgtcaga acctgttaaa accttacttt
     6901 ttacaaaaaa atccggcggc gaaagccttg aaaaaactta tgtggcaaaa aaattccgga
     6961 ctgattcgga ctttttcttt cctggccagg ccttctaatg gtcagtaagg tcagccgctt
     7021 cagcggtcaa tcgtgtataa tatagtcaag attgacaaga ggagggctga caatggaaaa
     7081 tagcgctggc atgctgtcag ttggtcaaat agctaaaatg ctgaagacca acagacagaa
     7141 catttacaac gtgctgaaaa ctgaacacat taaagcggac ggctacaatg acaagcacta
     7201 ttcactgtat agcccggaaa cgattcaaga gatcaaggcc gctctgtcta agaaagcaac
     7261 gctgagaagt aagaaggtag tagcaaaaga gcaggctgaa gagatagctg acttgaagaa
     7321 tcagctgtca gaacagcaga gattgacaac ctggctacag tctcagctgg ttcaacttca
     7381 agtagaggct gacaagctca ggagtcagaa cagccagtta cagctagacc acgcaaagac
     7441 tcagctcctt attggccagg ttgaccagga gaagacaaca ctgaaggccg aaaatgaccg
     7501 actgagcgct gaaaatgaca agctaggaca actaaccgac aaggtgctga aggacgctca
     7561 gagagcagaa gaggacgctc agaaggctaa agctgatcta gataaagccc ggcgggctgg
     7621 cttatggtct agaatcacca ggaattatta agagtggtat agccgttatc tgactttgtg
     7681 aaattcctta ttgggtctgt cagatcagcg atttaaaacc tatacgggtt tgtgaatcct
     7741 ggtttacgaa attgggcgat aaagaagccc gtaagtgcaa ggatagaagg ttagttccaa
     7801 taagacacat tatgtaaagt tgtaagtggt atacctgtaa ttgattgaca ggaactatac
     7861 ataggctaga cacttgtcag cattgactgt agcggcttta caatgacact agatctacac
     7921 tataattaca gtagaaagag attttgcacg catgacttac aaagaatgga ctgattcaga
     7981 tcatttagag ttagtcaaaa attggaaatt acacgggctg actaacgttg aaatagctca
     8041 aagaataggc attgctgaga agactctgta cgtatggttg aagaagtctc ctaagctgaa
     8101 gaaggccatt agaggcggca agaatattgc cagggctagg gctgagaatg cactgtatga
     8161 gcttgctctt aatggcgata ggcaagccct tttcttttgg ctcaaaaaca actacagaga
     8221 acgctactca gacaagccgt taagcccggc tgaagccgat ttgatgagtc agaaggcaag
     8281 gctggccaaa ttacaggctg acctggctga ggctcagctg aaggccatta aggaagacca
     8341 gggagaccaa gcaacgcaat taaacaacct gttagacagt ctgaaggaag ccgtgttaga
     8401 tgaggggatt agccccgata acatcgttcc tactggcaac ggcttaatta tcgatgatat
     8461 ttctgactct taggtttaca cgacattgac agtgtaaaca caagatagcg gaaaagattc
     8521 tgattattat atttacaagc actgtatatt gtgctattct aagatgtgct aaacggattt
     8581 ggggaatgca actaactgct gaaaggtatc aacttttttg ttgcgctctt taattcttta
     8641 gcaaaaagct agatatcaaa aaagagcgag accgagtatt gcttcacggg ttcgctctta
     8701 tttttttatc tggctagttg cctactggta ctatactgac accctagcgg catgtttgcg
     8761 gtattgcact acagcggcaa caatggtaaa aataataata ggtaacaaaa aaagccttta
     8821 gtactggcaa tactagaggc gggctgtgtt tagctctggt aaagcttaac acggttagag
     8881 ttgtagagtt gttgtatttg tctttacgga attatgatac gtttaaaagt agcaccttgt
     8941 caagggtaac gtatcacttc taaagtatat atagtagttt tcttctaaca ttatgttgta
     9001 aaaacctaac attttgtaga caaacactat acttctatga ctctaaccat gtttaagaca
     9061 ggccaggcta acaccctact ggcctgtttt ttgttgccaa aatttcaaaa gaaaggcggt
     9121 aacaaccgtg attagacaaa acagcgatgt tagagcggct attaaagctt ctggcttgaa
     9181 gcaatatgag gtagctactt tgatgaatgt ttcagccagc tatctcagcc agctgttact
     9241 acaaccattg tcagaaggcc ataaaaaacg cattatggcg gcgattaaac aaggtgagtc
     9301 attgaaggga gaacaagaat aatgatgagc ttagaagagc gtgagcgtga gattgaaaag
     9361 gtagtacgca ttgctgaagc tgacttcaac aacgcttatc acttgaatgc tatcaacaag
     9421 gaagacgtta ttaagaaccg ggcttacaag tacgctgaag tgctgaagct tcaagaattg
     9481 ctggcattga acaagaccat taaggacggg ctgaatggta ttgaaatgtc agtagatctc
     9541 attgagtagc ggggagaccc gctatgaaca atagtgaaaa gacttctcta atggctgagc
     9601 cgtacaactc aaacagaaac gccattgaca gactcagagt caaccagaag gccttacagg
     9661 ctggctctgt caagcgtgaa gagaattata tagccgatga atccggcaat tcagagggct
     9721 tcagaatggc cttctacacg gcatataaga gcggcgttca gttagctata ccagtagagg
     9781 ctgagcccgg aaaaatggta attaatgaga ccttcagcaa ggttcaacat ctactaattg
     9841 ccagctggta tagccaggct gacagagcaa gcaacttcag aatacagctt accttcaaag
     9901 agatctcaga ggcgctagga tacagcagaa gccaggctac agcgctcaga aaacagctca
     9961 gagacctaat tacaccgcta gtccgctgta cttttgttac cagcaataaa gacagcatag
    10021 acgctgtaaa cctttttgcg gctggtagct acagcaaggg acagctgaca atgtggttaa
    10081 ctcctaacat ggctgaaacg tttctgtcag aagaatcatc tacagaatat tttccgttat
    10141 cgttactgaa gctaaaaggg acagcctact acctggcctt gaagatcatg cacaacgcaa
    10201 acattaatgc acgctggcgt gctgacaggg ttgacagatt gggcttagaa gacacgctga
    10261 aggccttgcc tacactcccc gacccggtag agaatgcaca aggaactagt agacacttac
    10321 agcgccaaat catagccccc ctggctaagg ctattgaaga gcttgaggcc gtcactggca
    10381 ttgtcgttag acctagccag ccgctaaagg gactgaaggc gaaagatctg tctaaagtca
    10441 ctttgaatgt cactgattgg ggacaggttg acatagcaga attgaccaga aaaaagcgaa
    10501 aacgtttgcg aaaaaataat gttcgtgagg actaaaacta tattttttct aattcgtatg
    10561 taggtaatta tggccgcaaa tgtaggtaat tatggccgca aatgtaggta attatggccg
    10621 cattgtgaaa tttgggcaag tgcctttagg ccttgagcca gtaaggagta agcgtatttt
    10681 ttaaaaaagt ttcacttgtt aatagtttaa tagtattaaa agcaacggca cagcttgacg
    10741 ctggccttgc ttgaaaattg aaaaaagatg aaacagccag ggagagcaga gacttcttac
    10801 tggcttgttt tctaaacgag gtatctagca tgaacaactt agttaaacca gcagatttaa
    10861 agagcttgat ctctttaccg gaatacattg ccagcgtggt cagcatggac tctaaaggct
    10921 tctttagctg tgtcaatccg aaccaccccg acaagcatcc tagcatgtgt gtagatcaga
    10981 accacccgca atatgttcat tgcttcagtt gccaggccag ttatgacctg tttgactgtt
    11041 gggcgctgat taatgacggc gtgacagaga ccaggaagaa tagtgctggc aaggaaaagc
    11101 cagtctacaa cttcaacgct gtagcaatag agatagctga ccattacgga tataccctta
    11161 ttggcgaccc ggcaaataac ttgccagaac cgccattacc cgaaccagaa ccagagccag
    11221 ctcaaactca gaccagcaat gacagcaata tcagagccca attagaagcc tggcatgcta
    11281 gcttgaatca gactgactat cttcagaagc ggggaatcac tcagacaaca gcagagattt
    11341 tcaatttagg ctactcagca ctgaccaaca gcattattat tccttacggt caggacggct
    11401 attacgttca gagagcaatt aatccgattg agaaacatga ccgctacaga ttccctattg
    11461 gccaggtgag agtctacaac gctgaggcgc tgaaagaatg caagacggta ttcattgttg
    11521 aaggtcagtt tgacgctctg tcaatcatgc aagaatccgg tgtaggagct gtagcaactt
    11581 caaccagtca gacccggctt attgtcaaga ccttacaaaa gttcaaagag caagacccaa
    11641 caattaaccc gactatcatt ctcagtatgg acaacgacag agcaggccag aaggcgaata
    11701 gagcccttca gagggactta gaagcgcatg gctttacttg ctatgtcaac ccggttaacg
    11761 gcgattataa ggacgctaac gagtttctgg taaaggatag agagggcttc agacagaagc
    11821 ttcagcatgt catcaaccag cccgacaact ggcttgacaa gtactatgct gacatcaaaa
    11881 accgccatga ctacccggat aatatcccta ctggcttcaa gaatttagat gatgagcttg
    11941 acggcgggct tcagcctaaa ctctatgttt taggtgctgt cagttcatta gggaaaacga
    12001 ctttcgcctt gaatattgct gacaacctgg ctaaacaggg gagacatgtt ttcttcttca
    12061 gcatggaatc tagcaagaga gaagtgacgg acaagctttt aagccgggct agctgtctct
    12121 ctaacggcca taaatggact cagctacaag tcagccgggg agcatggtta aacaatgctg
    12181 aggacaaaga agagtttgac ggcctgttta aagccttcag ccgttaccag cgcttcttac
    12241 acatctatga caacagggtt aaggcaagtc aggtaaaaga cctggtcaat agctggcttg
    12301 gcaaccaccc ggacgaaaag aagccgcttg tagtcgttga ctatcttcag atcttgcaag
    12361 ctgagcagga caacgtgaca gacaaggcca aggtgacaga cagcgtgagt gttctctcag
    12421 agctgactaa acaggctgaa gttcctgtct tggtcatctc atcattgaac cgggcttctt
    12481 actggcaaga cgtaagtttt gaatccttca aggaatccgg ggaaatcgaa tactcagccg
    12541 atgttatgtt aggtttggaa tttgctcatc gtgaggagta cattacagtt cagaaaaacg
    12601 gccatgttga attgaacaaa gagaagtttg accagcggaa acaggaagtc cctagacggg
    12661 ttgaaatggt cattctgaag aatcgaactg gcaagacagg cggtcatatc ttcttcaagt
    12721 acaacgccat gtttaacagt taccaggcat gcactgagaa ggaagcggcg atagccaaca
    12781 acttcaaaat gctgtatcac acaaaacaag taggcaagcc agttgaggcg gctagaattg
    12841 aatacacggt agacccggaa acaggccggg taacagagaa gaatcaagat aaatagagct
    12901 aaagaagctg gccaggaatg gctggctttt gttttgcctt cagacgctct cagaagctca
    12961 tagagccgtt ctgagcctgg cctggtagat ttggccataa acccggctaa atacccccat
    13021 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
    13081 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
    13141 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
    13201 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
    13261 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacg
//
