LOCUS       V007941                11907 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007941
VERSION     V007941
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11907)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Significant reduction of BiFC non-specific assembly facilitates in
            planta assessment of heterotrimeric G-protein interactors
  JOURNAL   Plant J. (2014) In press
   PUBMED   25187041
REFERENCE   2  (bases 1 to 11907)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2014) Department of Biology, The Pennsylvania
            State University, 208 Mueller Laboratory, University Park, PA 16802,
            USA
REFERENCE   3  (bases 1 to 11907)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11907)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.
            (2014) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-SEP-2014) Department of Biology, The Pennsylvania State
            University, 208 Mueller Laboratory, University Park, PA 16802, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    99..104
                     /label="non-unique SacI"
                     /note="non-unique SacI"
     terminator      complement(111..363)
                     /label="MAS terminator"
                     /note="mannopine synthase terminator"
     misc_feature    complement(373..406)
                     /label="BAR gene remnant"
                     /note="BAR gene remnant"
     misc_feature    407..412
                     /label="MCS2 KpnI site"
                     /note="MCS2 KpnI site"
     CDS             complement(416..931)
                     /note="RNA silencing suppressor p19 from Tomato bushy stunt
                     virus (strain Cherry). Accession#: P11690"
                     /label="RNA silencing suppressor p19"
     promoter        complement(935..1315)
                     /label="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     regulatory      1328..1965
                     /note="UBQ10; Arabidopsis ubiquitin10 promoter"
                     /regulatory_class="promoter"
     misc_difference 1481
                     /replace="g"
                     /label="killed PmlI (CACcTG)"
                     /note="killed PmlI (CACcTG)"
     misc_feature    1662..1965
                     /label="cryptic intron from Norris et al. 1993"
                     /note="cryptic intron from Norris et al. 1993"
     misc_difference 1894
                     /replace="t"
                     /label="killed XbaI (aCTAGA)"
                     /note="killed XbaI (aCTAGA)"
     primer_bind     1966..1990
                     /label="pDOE mcs1F with XhoI"
                     /note="pDOE mcs1F with XhoI"
     misc_feature    1978..1994
                     /label="XhoSac linker"
                     /note="XhoSac linker"
     misc_feature    2005..2059
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2061..2777
                     /label="mTurquoise2"
                     /note="enhanced monomeric variant of CFP (Goedhart et al.,
                     2012)"
     misc_feature    2778..2831
                     /label="MCS1 front end"
                     /note="MCS1 front end"
     misc_feature    2832..2853
                     /label="MCS1 back end"
                     /note="MCS1 back end"
     terminator      2864..3571
                     /label="OCS terminator"
                     /note="octopine synthase terminator"
     regulatory      3592..4229
                     /note="UBQ10; ubiquitin10 promoter"
                     /regulatory_class="promoter"
     misc_difference 3745
                     /replace="g"
                     /label="killed PmlI (CACcTG)"
                     /note="killed PmlI (CACcTG)"
     misc_difference 4158
                     /replace="t"
                     /label="killed XbaI (aCTAGA)"
                     /note="killed XbaI (aCTAGA)"
     primer_bind     4230..4254
                     /label="pDOE mcs3bF with Bsu36I"
                     /note="pDOE mcs3bF with Bsu36I"
     misc_feature    4237..4252
                     /label="Bsu361 linker"
                     /note="Bsu361 linker"
     regulatory      4253..4318
                     /label="TMV-Omega enhancer"
                     /note="TMV-Omega enhancer"
                     /regulatory_class="enhancer"
     misc_feature    4263..4317
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     misc_feature    4319..4366
                     /label="MCS3 front end"
                     /note="MCS3 front end"
     CDS             4367..5083
                     /label="mVenus"
                     /note="Venus YFP with monomerizing A206K mutation (Nagai et
                     al., 2002; Kremers et al., 2006)"
     misc_feature    5087..5104
                     /label="MCS3 back end"
                     /note="MCS3 back end"
     terminator      5106..5358
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5368..5384
                     /label="remnant from pFGC5941"
                     /note="remnant from pFGC5941"
     primer_bind     complement(5409..5425)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    5628..5652
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6953..7579
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             8011..9081
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      9150..9344
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9688..9828
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10014..10602)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10692..11483)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta cctcactctg
      421 actccttctc gaaggtttga gtaccttcag gacatcctct tgaaacatta gattcaactt
      481 caattggagc caactgcaaa agttcttgct tagacctaat tgccatctca cacaaatgtt
      541 gaagagttct tgatcctcca gaaacagtga tactaactcc cctgaaccta atagaataag
      601 tacatccgat ttgatcgaac ccaaagaatc ttgatgcagc atagttgact gaatctccag
      661 tccaagatcc aagcactcta tgcaatgatg cttcagttct atcgtatctg agatacctct
      721 taaacacaac ttttccgaaa ccccaagatt ccttgaaacc aagtggatta tcttgatttg
      781 agttcgtctc atcgttatgc aatctccact cagtccaaga tggactttca tcaggaagtt
      841 tgaaaggaga agttgtacct cctgatcctc catcccatct ttcagagtta gcttgttctc
      901 tagcatcatt tccttgtata gctcgttcca ttcgcgattt ggtgtatcga gattggttat
      961 gaaattcaga tgctagtgta atgtattggt aatttgggaa gatataatag gaagcaaggc
     1021 tatttatcca tttctgaaaa ggcgaaatgg cgtcaccgcg agcgtcacgc gcattccgtt
     1081 cttgctgtaa agcgttgttt ggtacacttt tgactagcga ggcttggcgt gtcagcgtat
     1141 ctattcaaaa gtcgttaatg gctgcggatc aagaaaaagt tggaatagaa acagaatacc
     1201 cgcgaaattc aggcccggtt gccatgtcct acacgccgaa ataaacgacc aaattagtag
     1261 aaaaataaaa actgactcgg atacttacgt cacgtcttgc gcactgattt gaaaaatctc
     1321 agaattcttg tcgacgagtc agtaataaac ggcgtcaaag tggttgcagc cggcacacac
     1381 gagtcgtgtt tatcaactca aagcacaaat acttttcctc aacctaaaaa taaggcaatt
     1441 agccaaaaac aactttgcgt gtaaacaacg ctcaatacac ctgtcatttt attattagct
     1501 attgcttcac cgccttagct ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct
     1561 tctataaaac aatacccaaa gagctcttct tcttcacaat tcagatttca atttctcaaa
     1621 atcttaaaaa ctttctctca attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt
     1681 attctctcaa aatcttcgat tttgttttcg ttcgatccca atttcgtata tgttctttgg
     1741 tttagattct gttaatctta gatcgaagac gattttctgg gtttgatcgt tagatatcat
     1801 cttaattctc gattagggtt tcatagatat catccgattt gttcaaataa tttgagtttt
     1861 gtcgaataat tactcttcga tttgtgattt ctaactagat ctggtgttag tttctagttt
     1921 gtgcgatcga atttgtcgat taatctgagt ttttctgatt aacagggaga ggacacgctc
     1981 gagtataaga gctctatttt tacaacaatt accaacaaca acaaacaaca aacaacatta
     2041 caattacatt tacaattaca atggtgagca agggcgagga gctgtttact ggagttgtgc
     2101 ctattcttgt tgaattggat ggcgacgtta atggacataa gttttctgtt agtggtgagg
     2161 gtgagggaga tgctacttat ggaaagctca ctttgaagtt tatttgtaca actggaaagt
     2221 tgccggttcc ttggccgact cttgtgacaa ctttgtcttg gggagttcaa tgttttgcta
     2281 gataccctga ccacatgaaa caacatgatt tctttaagtc tgctatgcct gaaggatatg
     2341 ttcaagagag aactatcttc tttaaggacg atggaaatta taagactaga gctgaagtga
     2401 agtttgaagg agatactttg gttaatagaa ttgagttgaa gggtattgac tttaaagaag
     2461 atggaaatat tttgggtcat aagttggagt ataattactt ttcagataat gtttatatta
     2521 ctgctgataa acaaaagaat ggaattaaag ctaattttaa gattagacat aatattgaag
     2581 atggtggagt tcaattggct gatcattatc agcaaaatac tcctattggt gatggaccag
     2641 ttcttttgcc tgataaccac tatttgtcta ctcaatctaa gttgtctaaa gaccctaatg
     2701 aaaagagaga tcacatggtt cttttggaat ttgttacagc tgctggaatt actctcggca
     2761 tggacgagct gtacaaggga gccagtggag gtgggtcagg atccatgggg cgcgccctag
     2821 gaactagtta atctagaccc gggggttaat taaccctaga gtcctgcttt aatgagatat
     2881 gcgagacgcc tatgatcgca tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa
     2941 cctgagcatg tgtagctcag atccttaccg ccggtttcgg ttcattctaa tgaatatatc
     3001 acccgttact atcgtatttt tatgaataat attctccgtt caatttactg attgtaccct
     3061 actacttata tgtacaatat taaaatgaaa acaatatatt gtgctgaata ggtttatagc
     3121 gacatctatg atagagcgcc acaataacaa acaattgcgt tttattatta caaatccaat
     3181 tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg attacataaa tcttattcaa
     3241 atttcaaaag tgccccaggg gctagtatct acgacacacc gagcggcgaa ctaataacgc
     3301 tcactgaagg gaactccggt tccccgccgg cgcgcatggg tgagattcct tgaagttgag
     3361 tattggccgt ccgctctacc gaaagttacg ggcaccattc aacccggtcc agcacggcgg
     3421 ccgggtaacc gacttgctgc cccgagaatt atgcagcatt tttttggtgt atgtgggccc
     3481 caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg gtccagggcg
     3541 aattttgcga caacatgtcg aggctcagca ggacctgctc caagagtatg gttgtcgacg
     3601 agtcagtaat aaacggcgtc aaagtggttg cagccggcac acacgagtcg tgtttatcaa
     3661 ctcaaagcac aaatactttt cctcaaccta aaaataaggc aattagccaa aaacaacttt
     3721 gcgtgtaaac aacgctcaat acacctgtca ttttattatt agctattgct tcaccgcctt
     3781 agctttctcg tgacctagtc gtcctcgtct tttcttcttc ttcttctata aaacaatacc
     3841 caaagagctc ttcttcttca caattcagat ttcaatttct caaaatctta aaaactttct
     3901 ctcaattctc tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc tcaaaatctt
     3961 cgattttgtt ttcgttcgat cccaatttcg tatatgttct ttggtttaga ttctgttaat
     4021 cttagatcga agacgatttt ctgggtttga tcgttagata tcatcttaat tctcgattag
     4081 ggtttcatag atatcatccg atttgttcaa ataatttgag ttttgtcgaa taattactct
     4141 tcgatttgtg atttctaact agatctggtg ttagtttcta gtttgtgcga tcgaatttgt
     4201 cgattaatct gagtttttct gattaacagg gacacgcctc aggtacagag catattttta
     4261 caacaattac caacaacaac aaacaacaaa caacattaca attacattta caattacaat
     4321 ggggtcccta cgtagtcacg tgacgtccgg aggttctggt ggaggtatgg tgagcaaggg
     4381 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     4441 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     4501 gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     4561 gggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
     4621 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     4681 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     4741 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     4801 ctacaacagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
     4861 cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca
     4921 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
     4981 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     5041 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagcga tcgccattta
     5101 aataggatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     5161 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     5221 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     5281 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     5341 catctatgtt actagatcgt taacgatatg tcgaggctca gcaggacctg caggcatgca
     5401 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     5461 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     5521 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc
     5581 ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
     5641 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
     5701 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
     5761 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
     5821 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
     5881 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
     5941 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
     6001 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
     6061 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
     6121 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac
     6181 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
     6241 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
     6301 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
     6361 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
     6421 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
     6481 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc
     6541 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
     6601 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
     6661 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
     6721 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc
     6781 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
     6841 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
     6901 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
     6961 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
     7021 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
     7081 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
     7141 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
     7201 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
     7261 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
     7321 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
     7381 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
     7441 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
     7501 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
     7561 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
     7621 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
     7681 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
     7741 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
     7801 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
     7861 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
     7921 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gcctgccggc cctgcaatgg
     7981 cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta
     8041 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
     8101 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
     8161 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
     8221 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
     8281 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
     8341 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
     8401 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
     8461 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
     8521 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
     8581 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
     8641 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
     8701 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
     8761 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
     8821 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
     8881 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
     8941 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
     9001 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
     9061 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
     9121 ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag
     9181 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
     9241 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
     9301 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
     9361 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
     9421 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
     9481 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
     9541 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     9601 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     9661 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
     9721 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     9781 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     9841 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9901 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9961 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    10021 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    10081 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    10141 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    10201 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    10261 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    10321 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10381 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    10441 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10501 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10561 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10621 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
    10681 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc
    10741 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg
    10801 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga
    10861 tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg
    10921 tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc
    10981 ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta
    11041 ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc
    11101 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca
    11161 gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc
    11221 agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct
    11281 tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg
    11341 ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata taccttagca
    11401 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc
    11461 ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga
    11521 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
    11581 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
    11641 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
    11701 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
    11761 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
    11821 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
    11881 ccggtcgggg agctgttggc tggctgg
//
