LOCUS       40924_14965       11595 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pDOE-12, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Significant reduction of BiFC non-specific assembly facilitates in 
            planta assessment of heterotrimeric G-protein interactors
  JOURNAL   Plant J. (2014) In press
  PUBMED    25187041
REFERENCE   2  (bases 1 to 11595)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2014) Department of Biology, The Pennsylvania 
            State University, 208 Mueller Laboratory, University Park, PA 16802,
            USA
REFERENCE   3  (bases 1 to 11595)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11595)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-SEP-2014) Department of Biology, The Pennsylvania State 
            University, 208 Mueller Laboratory, University Park, PA 16802, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    99..104
                     /label=non-unique SacI
                     /note="non-unique SacI"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    complement(373..406)
                     /label=BAR gene remnant
                     /note="BAR gene remnant"
     misc_feature    407..412
                     /label=MCS2 KpnI site
                     /note="MCS2 KpnI site"
     CDS             complement(416..1129)
                     /label=mTurquoise2
                     /note="enhanced monomeric variant of CFP (Goedhart et al., 
                     2012)"
     sig_peptide     complement(1130..1234)
                     /gene="XT-Golgi-mTq2"
                     /note="exclusive localization to the medial Golgi
                     cisternae; from Arabidopsis AtXylT/XYLT 
                     (beta-1,2-xylosyltransferase, At5g55500); characterized in 
                     PubMed ID 12943552; confirmed by Saint-Jore-Dupas et al. 
                     (Plant Cell 2006;18;3182-3200)"
     misc_feature    1235..1240
                     /label=MCS2 NruI site
                     /note="MCS2 NruI site"
     regulatory      complement(1238..1618)
                     /note="mas1'-2' mannopine synthase promoter; mas2' drives
                     MCS2 ORF; mas1' truncated by 96 nt; enhancer elements, TATA
                     box, and start codon missing"
                     /regulatory_class="promoter"
     promoter        complement(1238..1618)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     regulatory      1631..2268
                     /note="UBQ10; Arabidopsis ubiquitin10 promoter"
                     /regulatory_class="promoter"
     misc_difference 1784
                     /replace="g"
                     /label=killed PmlI (CACcTG)
                     /note="killed PmlI (CACcTG)"
     misc_feature    1965..2268
                     /label=cryptic intron from Norris et al. 1993
                     /note="cryptic intron from Norris et al. 1993"
     misc_difference 2197
                     /replace="t"
                     /label=killed XbaI (aCTAGA)
                     /note="killed XbaI (aCTAGA)"
     primer_bind     2269..2293
                     /label=pDOE mcs1F with XhoI
                     /note="pDOE mcs1F with XhoI"
     misc_feature    2281..2297
                     /label=XhoSac linker
                     /note="XhoSac linker"
     misc_feature    2308..2362
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2364..3047
                     /codon_start=1
                     /gene="NmVen210-X"
                     /product="NmVenus210-mcs1 fusion protein"
                     /label=NmVen210-X
                     /protein_id="AIN46649.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKGASGGGSGSMGR
                     ALGTS"
     gene            2364..3047
                     /gene="NmVen210-X"
                     /label=NmVen210-X
     misc_feature    2364..2993
                     /gene="NmVen210-X"
                     /label=NmVenus210 specific sequence
                     /note="NmVenus210 specific sequence"
     CDS             2364..2882
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     misc_difference 2982..2984
                     /gene="NmVen210-X"
                     /label=A206K monomerizing mutation
                     /note="A206K monomerizing mutation"
     misc_feature    2994..3047
                     /label=MCS1 front end
                     /note="MCS1 front end"
     misc_feature    3048..3069
                     /label=MCS1 back end
                     /note="MCS1 back end"
     terminator      3080..3787
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     regulatory      3808..4445
                     /note="UBQ10; ubiquitin10 promoter"
                     /regulatory_class="promoter"
     misc_difference 3961
                     /replace="g"
                     /label=killed PmlI (CACcTG)
                     /note="killed PmlI (CACcTG)"
     misc_difference 4374
                     /replace="t"
                     /label=killed XbaI (aCTAGA)
                     /note="killed XbaI (aCTAGA)"
     primer_bind     4446..4470
                     /label=pDOE mcs3bF with Bsu36I
                     /note="pDOE mcs3bF with Bsu36I"
     misc_feature    4453..4468
                     /label=Bsu361 linker
                     /note="Bsu361 linker"
     regulatory      4469..4534
                     /label=TMV-Omega enhancer
                     /note="TMV-Omega enhancer"
                     /regulatory_class="enhancer"
     misc_feature    4479..4533
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     misc_feature    4538..4624
                     /gene="CVen210-SFS-X"
                     /label=CVenus210 specific sequence
                     /note="CVenus210 specific sequence"
     CDS             4637..4660
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             4670..4693
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             4700..4723
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     misc_feature    4739..4774
                     /label=MCS3 front end
                     /note="MCS3 front end"
     misc_feature    4775..4792
                     /label=MCS3 back end
                     /note="MCS3 back end"
     terminator      4794..5046
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5056..5072
                     /label=remnant from pFGC5941
                     /note="remnant from pFGC5941"
     primer_bind     complement(5097..5113)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5316..5340
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6641..7267
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             7699..8769
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      8838..9032
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9376..9516
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9702..10290)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10380..11171)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta cctcacttgt
      421 acagctcgtc catgccgaga gtaattccag cagctgtaac aaattccaaa agaaccatgt
      481 gatctctctt ttcattaggg tctttagaca acttagattg agtagacaaa tagtggttat
      541 caggcaaaag aactggtcca tcaccaatag gagtattttg ctgataatga tcagccaatt
      601 gaactccacc atcttcaata ttatgtctaa tcttaaaatt agctttaatt ccattctttt
      661 gtttatcagc agtaatataa acattatctg aaaagtaatt atactccaac ttatgaccca
      721 aaatatttcc atcttcttta aagtcaatac ccttcaactc aattctatta accaaagtat
      781 ctccttcaaa cttcacttca gctctagtct tataatttcc atcgtcctta aagaagatag
      841 ttctctcttg aacatatcct tcaggcatag cagacttaaa gaaatcatgt tgtttcatgt
      901 ggtcagggta tctagcaaaa cattgaactc cccaagacaa agttgtcaca agagtcggcc
      961 aaggaaccgg caactttcca gttgtacaaa taaacttcaa agtgagcttt ccataagtag
     1021 catctccctc accctcacca ctaacagaaa acttatgtcc attaacgtcg ccatccaatt
     1081 caacaagaat aggcacaact ccagtaaaca gctcctcgcc cttgctcact gagaaagatg
     1141 aactgtggaa cacgaagtag atgataagga acaaactgtt gagaagcaac atatacagga
     1201 atatcttcag aatcttaggg ttccgcttac tcattcgcga tttggtgtat cgagattggt
     1261 tatgaaattc agatgctagt gtaatgtatt ggtaatttgg gaagatataa taggaagcaa
     1321 ggctatttat ccatttctga aaaggcgaaa tggcgtcacc gcgagcgtca cgcgcattcc
     1381 gttcttgctg taaagcgttg tttggtacac ttttgactag cgaggcttgg cgtgtcagcg
     1441 tatctattca aaagtcgtta atggctgcgg atcaagaaaa agttggaata gaaacagaat
     1501 acccgcgaaa ttcaggcccg gttgccatgt cctacacgcc gaaataaacg accaaattag
     1561 tagaaaaata aaaactgact cggatactta cgtcacgtct tgcgcactga tttgaaaaat
     1621 ctcagaattc ttgtcgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca
     1681 cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca
     1741 attagccaaa aacaactttg cgtgtaaaca acgctcaata cacctgtcat tttattatta
     1801 gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct
     1861 tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc
     1921 aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc
     1981 cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt
     2041 tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat
     2101 catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt
     2161 tttgtcgaat aattactctt cgatttgtga tttctaacta gatctggtgt tagtttctag
     2221 tttgtgcgat cgaatttgtc gattaatctg agtttttctg attaacaggg agaggacacg
     2281 ctcgagtata agagctctat ttttacaaca attaccaaca acaacaaaca acaaacaaca
     2341 ttacaattac atttacaatt acaatggtga gcaagggcga ggagctgttc accggggtgg
     2401 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2461 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca
     2521 agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg
     2581 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     2641 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2701 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2761 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2821 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     2881 aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2941 ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc aaaggagcca
     3001 gtggaggtgg gtcaggatcc atggggcgcg ccctaggaac tagttaatct agacccgggg
     3061 gttaattaac cctagagtcc tgctttaatg agatatgcga gacgcctatg atcgcatgat
     3121 atttgctttc aattctgttg tgcacgttgt aaaaaacctg agcatgtgta gctcagatcc
     3181 ttaccgccgg tttcggttca ttctaatgaa tatatcaccc gttactatcg tatttttatg
     3241 aataatattc tccgttcaat ttactgattg taccctacta cttatatgta caatattaaa
     3301 atgaaaacaa tatattgtgc tgaataggtt tatagcgaca tctatgatag agcgccacaa
     3361 taacaaacaa ttgcgtttta ttattacaaa tccaatttta aaaaaagcgg cagaaccggt
     3421 caaacctaaa agactgatta cataaatctt attcaaattt caaaagtgcc ccaggggcta
     3481 gtatctacga cacaccgagc ggcgaactaa taacgctcac tgaagggaac tccggttccc
     3541 cgccggcgcg catgggtgag attccttgaa gttgagtatt ggccgtccgc tctaccgaaa
     3601 gttacgggca ccattcaacc cggtccagca cggcggccgg gtaaccgact tgctgccccg
     3661 agaattatgc agcatttttt tggtgtatgt gggccccaaa tgaagtgcag gtcaaacctt
     3721 gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt ttgcgacaac atgtcgaggc
     3781 tcagcaggac ctgctccaag agtatggttg tcgacgagtc agtaataaac ggcgtcaaag
     3841 tggttgcagc cggcacacac gagtcgtgtt tatcaactca aagcacaaat acttttcctc
     3901 aacctaaaaa taaggcaatt agccaaaaac aactttgcgt gtaaacaacg ctcaatacac
     3961 ctgtcatttt attattagct attgcttcac cgccttagct ttctcgtgac ctagtcgtcc
     4021 tcgtcttttc ttcttcttct tctataaaac aatacccaaa gagctcttct tcttcacaat
     4081 tcagatttca atttctcaaa atcttaaaaa ctttctctca attctctcta ccgtgatcaa
     4141 ggtaaatttc tgtgttcctt attctctcaa aatcttcgat tttgttttcg ttcgatccca
     4201 atttcgtata tgttctttgg tttagattct gttaatctta gatcgaagac gattttctgg
     4261 gtttgatcgt tagatatcat cttaattctc gattagggtt tcatagatat catccgattt
     4321 gttcaaataa tttgagtttt gtcgaataat tactcttcga tttgtgattt ctaactagat
     4381 ctggtgttag tttctagttt gtgcgatcga atttgtcgat taatctgagt ttttctgatt
     4441 aacagggaca cgcctcaggt acagagcata tttttacaac aattaccaac aacaacaaac
     4501 aacaaacaac attacaatta catttacaat tacaatggac cccaacgaga agcgcgatca
     4561 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     4621 caagggatct gggtcatggt ctcatccaca attcgagaag tcaggaggag attataagga
     4681 tgatgatgat aaggcttctt ggtcacatcc tcagtttgaa aagtctggag cttcaggaat
     4741 ggggtcccta cgtagtcacg tgacgtccgg ataagcgatc gccatttaaa taggatcgtt
     4801 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta
     4861 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
     4921 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
     4981 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
     5041 tagatcgtta acgatatgtc gaggctcagc aggacctgca ggcatgcaag cttggcactg
     5101 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     5161 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     5221 tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt
     5281 gtcgtttccc gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
     5341 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
     5401 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact
     5461 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
     5521 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
     5581 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac
     5641 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc
     5701 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
     5761 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
     5821 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
     5881 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
     5941 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
     6001 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
     6061 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
     6121 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
     6181 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
     6241 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc
     6301 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
     6361 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
     6421 tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt
     6481 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc
     6541 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
     6601 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
     6661 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
     6721 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
     6781 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga
     6841 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
     6901 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
     6961 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga
     7021 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
     7081 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg
     7141 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
     7201 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
     7261 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc
     7321 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca
     7381 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac
     7441 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
     7501 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
     7561 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
     7621 cggttggcca ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc
     7681 caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg
     7741 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc
     7801 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
     7861 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
     7921 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
     7981 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
     8041 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt
     8101 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
     8161 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
     8221 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
     8281 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
     8341 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
     8401 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
     8461 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
     8521 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
     8581 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaaaaa gttctgtttc
     8641 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
     8701 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
     8761 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
     8821 cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
     8881 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
     8941 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
     9001 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
     9061 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
     9121 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
     9181 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
     9241 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     9301 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     9361 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     9421 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     9481 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     9541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9721 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9781 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9841 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9901 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9961 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10021 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10081 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10141 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10201 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10261 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10321 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtactaa
    10381 aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt
    10441 aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg
    10501 cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca
    10561 cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca
    10621 agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat
    10681 ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa
    10741 tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg
    10801 gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca
    10861 tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag
    10921 ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc
    10981 atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt
    11041 tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct
    11101 tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc
    11161 attttagcca tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag
    11221 ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc
    11281 agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc
    11341 cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg
    11401 ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa
    11461 tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc
    11521 ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag
    11581 ctgttggctg gctgg
//
