LOCUS       40924_14960       11592 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pDOE-11, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Significant reduction of BiFC non-specific assembly facilitates in 
            planta assessment of heterotrimeric G-protein interactors
  JOURNAL   Plant J. (2014) In press
  PUBMED    25187041
REFERENCE   2  (bases 1 to 11592)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2014) Department of Biology, The Pennsylvania 
            State University, 208 Mueller Laboratory, University Park, PA 16802,
            USA
REFERENCE   3  (bases 1 to 11592)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11592)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-SEP-2014) Department of Biology, The Pennsylvania State 
            University, 208 Mueller Laboratory, University Park, PA 16802, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    99..104
                     /label=non-unique SacI
                     /note="non-unique SacI"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    complement(373..406)
                     /label=BAR gene remnant
                     /note="BAR gene remnant"
     misc_feature    407..412
                     /label=MCS2 KpnI site
                     /note="MCS2 KpnI site"
     CDS             complement(416..1129)
                     /label=mTurquoise2
                     /note="enhanced monomeric variant of CFP (Goedhart et al., 
                     2012)"
     sig_peptide     complement(1130..1234)
                     /gene="XT-Golgi-mTq2"
                     /note="exclusive localization to the medial Golgi
                     cisternae; from Arabidopsis AtXylT/XYLT 
                     (beta-1,2-xylosyltransferase, At5g55500); characterized in 
                     PubMed ID 12943552; confirmed by Saint-Jore-Dupas et al. 
                     (Plant Cell 2006;18;3182-3200)"
     misc_feature    1235..1240
                     /label=MCS2 NruI site
                     /note="MCS2 NruI site"
     regulatory      complement(1238..1618)
                     /note="mas1'-2' mannopine synthase promoter; mas2' drives
                     MCS2 ORF; mas1' truncated by 96 nt; enhancer elements, TATA
                     box, and start codon missing"
                     /regulatory_class="promoter"
     promoter        complement(1238..1618)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     regulatory      1631..2268
                     /note="UBQ10; Arabidopsis ubiquitin10 promoter"
                     /regulatory_class="promoter"
     misc_difference 1784
                     /replace="g"
                     /label=killed PmlI (CACcTG)
                     /note="killed PmlI (CACcTG)"
     misc_feature    1965..2268
                     /label=cryptic intron from Norris et al. 1993
                     /note="cryptic intron from Norris et al. 1993"
     misc_difference 2197
                     /replace="t"
                     /label=killed XbaI (aCTAGA)
                     /note="killed XbaI (aCTAGA)"
     primer_bind     2269..2293
                     /label=pDOE mcs1F with XhoI
                     /note="pDOE mcs1F with XhoI"
     misc_feature    2281..2297
                     /label=XhoSac linker
                     /note="XhoSac linker"
     misc_feature    2308..2362
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2364..3047
                     /codon_start=1
                     /gene="NmVen210-X"
                     /product="NmVenus210-mcs1 fusion protein"
                     /label=NmVen210-X
                     /protein_id="AIN46645.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKGASGGGSGSMGR
                     ALGTS"
     gene            2364..3047
                     /gene="NmVen210-X"
                     /label=NmVen210-X
     misc_feature    2364..2993
                     /gene="NmVen210-X"
                     /label=NmVenus210 specific sequence
                     /note="NmVenus210 specific sequence"
     CDS             2364..2882
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     misc_difference 2982..2984
                     /gene="NmVen210-X"
                     /label=A206K monomerizing mutation
                     /note="A206K monomerizing mutation"
     misc_feature    2994..3047
                     /label=MCS1 front end
                     /note="MCS1 front end"
     misc_feature    3048..3069
                     /label=MCS1 back end
                     /note="MCS1 back end"
     terminator      3080..3787
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     regulatory      3808..4445
                     /note="UBQ10; ubiquitin10 promoter"
                     /regulatory_class="promoter"
     misc_difference 3961
                     /replace="g"
                     /label=killed PmlI (CACcTG)
                     /note="killed PmlI (CACcTG)"
     misc_difference 4374
                     /replace="t"
                     /label=killed XbaI (aCTAGA)
                     /note="killed XbaI (aCTAGA)"
     primer_bind     4446..4470
                     /label=pDOE mcs3bF with Bsu36I
                     /note="pDOE mcs3bF with Bsu36I"
     misc_feature    4453..4468
                     /label=Bsu361 linker
                     /note="Bsu361 linker"
     regulatory      4469..4534
                     /label=TMV-Omega enhancer
                     /note="TMV-Omega enhancer"
                     /regulatory_class="enhancer"
     misc_feature    4479..4533
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     misc_feature    4535..4582
                     /label=MCS3 front end
                     /note="MCS3 front end"
     CDS             4583..4606
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             4616..4639
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             4646..4669
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     misc_feature    4682..4771
                     /gene="X-SFS-CVen210"
                     /label=CVenus210 specific sequence
                     /note="CVenus210 specific sequence"
     misc_feature    4772..4790
                     /label=MCS3 back end
                     /note="MCS3 back end"
     terminator      4791..5043
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5053..5069
                     /label=remnant from pFGC5941
                     /note="remnant from pFGC5941"
     primer_bind     complement(5094..5110)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5313..5337
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6638..7264
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             7696..8766
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      8835..9029
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9373..9513
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9699..10287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10377..11168)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta cctcacttgt
      421 acagctcgtc catgccgaga gtaattccag cagctgtaac aaattccaaa agaaccatgt
      481 gatctctctt ttcattaggg tctttagaca acttagattg agtagacaaa tagtggttat
      541 caggcaaaag aactggtcca tcaccaatag gagtattttg ctgataatga tcagccaatt
      601 gaactccacc atcttcaata ttatgtctaa tcttaaaatt agctttaatt ccattctttt
      661 gtttatcagc agtaatataa acattatctg aaaagtaatt atactccaac ttatgaccca
      721 aaatatttcc atcttcttta aagtcaatac ccttcaactc aattctatta accaaagtat
      781 ctccttcaaa cttcacttca gctctagtct tataatttcc atcgtcctta aagaagatag
      841 ttctctcttg aacatatcct tcaggcatag cagacttaaa gaaatcatgt tgtttcatgt
      901 ggtcagggta tctagcaaaa cattgaactc cccaagacaa agttgtcaca agagtcggcc
      961 aaggaaccgg caactttcca gttgtacaaa taaacttcaa agtgagcttt ccataagtag
     1021 catctccctc accctcacca ctaacagaaa acttatgtcc attaacgtcg ccatccaatt
     1081 caacaagaat aggcacaact ccagtaaaca gctcctcgcc cttgctcact gagaaagatg
     1141 aactgtggaa cacgaagtag atgataagga acaaactgtt gagaagcaac atatacagga
     1201 atatcttcag aatcttaggg ttccgcttac tcattcgcga tttggtgtat cgagattggt
     1261 tatgaaattc agatgctagt gtaatgtatt ggtaatttgg gaagatataa taggaagcaa
     1321 ggctatttat ccatttctga aaaggcgaaa tggcgtcacc gcgagcgtca cgcgcattcc
     1381 gttcttgctg taaagcgttg tttggtacac ttttgactag cgaggcttgg cgtgtcagcg
     1441 tatctattca aaagtcgtta atggctgcgg atcaagaaaa agttggaata gaaacagaat
     1501 acccgcgaaa ttcaggcccg gttgccatgt cctacacgcc gaaataaacg accaaattag
     1561 tagaaaaata aaaactgact cggatactta cgtcacgtct tgcgcactga tttgaaaaat
     1621 ctcagaattc ttgtcgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca
     1681 cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca
     1741 attagccaaa aacaactttg cgtgtaaaca acgctcaata cacctgtcat tttattatta
     1801 gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct
     1861 tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc
     1921 aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc
     1981 cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt
     2041 tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat
     2101 catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt
     2161 tttgtcgaat aattactctt cgatttgtga tttctaacta gatctggtgt tagtttctag
     2221 tttgtgcgat cgaatttgtc gattaatctg agtttttctg attaacaggg agaggacacg
     2281 ctcgagtata agagctctat ttttacaaca attaccaaca acaacaaaca acaaacaaca
     2341 ttacaattac atttacaatt acaatggtga gcaagggcga ggagctgttc accggggtgg
     2401 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2461 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca
     2521 agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg
     2581 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     2641 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2701 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2761 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2821 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     2881 aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2941 ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc aaaggagcca
     3001 gtggaggtgg gtcaggatcc atggggcgcg ccctaggaac tagttaatct agacccgggg
     3061 gttaattaac cctagagtcc tgctttaatg agatatgcga gacgcctatg atcgcatgat
     3121 atttgctttc aattctgttg tgcacgttgt aaaaaacctg agcatgtgta gctcagatcc
     3181 ttaccgccgg tttcggttca ttctaatgaa tatatcaccc gttactatcg tatttttatg
     3241 aataatattc tccgttcaat ttactgattg taccctacta cttatatgta caatattaaa
     3301 atgaaaacaa tatattgtgc tgaataggtt tatagcgaca tctatgatag agcgccacaa
     3361 taacaaacaa ttgcgtttta ttattacaaa tccaatttta aaaaaagcgg cagaaccggt
     3421 caaacctaaa agactgatta cataaatctt attcaaattt caaaagtgcc ccaggggcta
     3481 gtatctacga cacaccgagc ggcgaactaa taacgctcac tgaagggaac tccggttccc
     3541 cgccggcgcg catgggtgag attccttgaa gttgagtatt ggccgtccgc tctaccgaaa
     3601 gttacgggca ccattcaacc cggtccagca cggcggccgg gtaaccgact tgctgccccg
     3661 agaattatgc agcatttttt tggtgtatgt gggccccaaa tgaagtgcag gtcaaacctt
     3721 gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt ttgcgacaac atgtcgaggc
     3781 tcagcaggac ctgctccaag agtatggttg tcgacgagtc agtaataaac ggcgtcaaag
     3841 tggttgcagc cggcacacac gagtcgtgtt tatcaactca aagcacaaat acttttcctc
     3901 aacctaaaaa taaggcaatt agccaaaaac aactttgcgt gtaaacaacg ctcaatacac
     3961 ctgtcatttt attattagct attgcttcac cgccttagct ttctcgtgac ctagtcgtcc
     4021 tcgtcttttc ttcttcttct tctataaaac aatacccaaa gagctcttct tcttcacaat
     4081 tcagatttca atttctcaaa atcttaaaaa ctttctctca attctctcta ccgtgatcaa
     4141 ggtaaatttc tgtgttcctt attctctcaa aatcttcgat tttgttttcg ttcgatccca
     4201 atttcgtata tgttctttgg tttagattct gttaatctta gatcgaagac gattttctgg
     4261 gtttgatcgt tagatatcat cttaattctc gattagggtt tcatagatat catccgattt
     4321 gttcaaataa tttgagtttt gtcgaataat tactcttcga tttgtgattt ctaactagat
     4381 ctggtgttag tttctagttt gtgcgatcga atttgtcgat taatctgagt ttttctgatt
     4441 aacagggaca cgcctcaggt acagagcata tttttacaac aattaccaac aacaacaaac
     4501 aacaaacaac attacaatta catttacaat tacaatgggg tccctacgta gtcacgtgac
     4561 gtccggaggt tctggtggag cttggtctca tccacaattc gagaagtcag gaggagatta
     4621 taaggatgat gatgataagg cttcttggtc acatcctcag tttgaaaagg gatctgggtc
     4681 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4741 cactctcggc atggacgagc tgtacaagta agcgatcgcc atttaaatag gatcgttcaa
     4801 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     4861 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     4921 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     4981 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     5041 atcgttaacg atatgtcgag gctcagcagg acctgcaggc atgcaagctt ggcactggcc
     5101 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     5161 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5221 caacagttgc gcagcctgaa tggcgaatgc tagagcagct tgagcttgga tcagattgtc
     5281 gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     5341 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
     5401 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
     5461 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
     5521 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
     5581 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
     5641 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
     5701 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
     5761 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
     5821 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
     5881 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
     5941 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
     6001 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
     6061 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
     6121 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
     6181 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
     6241 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
     6301 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
     6361 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
     6421 tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
     6481 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
     6541 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
     6601 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
     6661 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
     6721 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
     6781 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
     6841 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
     6901 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
     6961 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
     7021 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
     7081 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
     7141 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
     7201 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
     7261 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
     7321 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
     7381 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
     7441 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
     7501 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
     7561 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
     7621 ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa
     7681 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
     7741 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc
     7801 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
     7861 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag
     7921 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
     7981 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
     8041 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg
     8101 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
     8161 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
     8221 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
     8281 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
     8341 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
     8401 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa
     8461 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
     8521 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
     8581 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
     8641 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
     8701 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
     8761 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
     8821 aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
     8881 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
     8941 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
     9001 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg
     9061 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
     9121 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
     9181 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
     9241 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     9301 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     9361 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     9421 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     9481 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     9541 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9601 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9661 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9721 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9781 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9841 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9901 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9961 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    10021 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10081 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
    10141 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10201 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10261 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10321 gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac
    10381 aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag
    10441 tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag
    10501 aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt
    10561 actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt
    10621 tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga
    10681 gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc
    10741 aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag
    10801 tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct
    10861 tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca
    10921 tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc
    10981 atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt
    11041 aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc
    11101 gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt
    11161 ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
    11221 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
    11281 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
    11341 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
    11401 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
    11461 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
    11521 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
    11581 ttggctggct gg
//
