LOCUS       40924_14950       11589 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pDOE-09, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11589)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Significant reduction of BiFC non-specific assembly facilitates in 
            planta assessment of heterotrimeric G-protein interactors
  JOURNAL   Plant J. (2014) In press
  PUBMED    25187041
REFERENCE   2  (bases 1 to 11589)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2014) Department of Biology, The Pennsylvania 
            State University, 208 Mueller Laboratory, University Park, PA 16802,
            USA
REFERENCE   3  (bases 1 to 11589)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11589)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-SEP-2014) Department of Biology, The Pennsylvania State 
            University, 208 Mueller Laboratory, University Park, PA 16802, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    99..104
                     /label=non-unique SacI
                     /note="non-unique SacI"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    complement(373..406)
                     /label=BAR gene remnant
                     /note="BAR gene remnant"
     misc_feature    407..412
                     /label=MCS2 KpnI site
                     /note="MCS2 KpnI site"
     CDS             complement(416..1129)
                     /label=mTurquoise2
                     /note="enhanced monomeric variant of CFP (Goedhart et al., 
                     2012)"
     sig_peptide     complement(1130..1234)
                     /gene="XT-Golgi-mTq2"
                     /note="exclusive localization to the medial Golgi
                     cisternae; from Arabidopsis AtXylT/XYLT 
                     (beta-1,2-xylosyltransferase, At5g55500); characterized in 
                     PubMed ID 12943552; confirmed by Saint-Jore-Dupas et al. 
                     (Plant Cell 2006;18;3182-3200)"
     misc_feature    1235..1240
                     /label=MCS2 NruI site
                     /note="MCS2 NruI site"
     regulatory      complement(1238..1618)
                     /note="mas1'-2' mannopine synthase promoter; mas2' drives
                     MCS2 ORF; mas1' truncated by 96 nt; enhancer elements, TATA
                     box, and start codon missing"
                     /regulatory_class="promoter"
     promoter        complement(1238..1618)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     regulatory      1631..2268
                     /note="UBQ10; Arabidopsis ubiquitin10 promoter"
                     /regulatory_class="promoter"
     misc_difference 1784
                     /replace="g"
                     /label=killed PmlI (CACcTG)
                     /note="killed PmlI (CACcTG)"
     misc_feature    1965..2268
                     /label=cryptic intron from Norris et al. 1993
                     /note="cryptic intron from Norris et al. 1993"
     misc_difference 2197
                     /replace="t"
                     /label=killed XbaI (aCTAGA)
                     /note="killed XbaI (aCTAGA)"
     primer_bind     2269..2293
                     /label=pDOE mcs1F with XhoI
                     /note="pDOE mcs1F with XhoI"
     misc_feature    2281..2297
                     /label=XhoSac linker
                     /note="XhoSac linker"
     misc_feature    2308..2362
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2364..3044
                     /codon_start=1
                     /gene="X-NmVen210"
                     /product="mcs1-NmVenus210 fusion protein"
                     /label=X-NmVen210
                     /protein_id="AIN46637.1"
                     /translation="MGRALGTSGGSGGGSGMVSKGEELFTGVVPILVELDGDVNGHKFS
                     VSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSA
                     MPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYN
                     SHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQS
                     KLSK"
     gene            2364..3044
                     /gene="X-NmVen210"
                     /label=X-NmVen210
     misc_feature    2364..2411
                     /label=MCS1 front end
                     /note="MCS1 front end"
     misc_feature    2412..3044
                     /gene="X-NmVen210"
                     /label=NmVenus210 specific sequence
                     /note="NmVenus210 specific sequence"
     CDS             2412..2930
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     misc_difference 3030..3032
                     /gene="X-NmVen210"
                     /label=A206K monomerizing mutation
                     /note="A206K monomerizing mutation"
     misc_feature    3045..3066
                     /label=MCS1 back end
                     /note="MCS1 back end"
     terminator      3077..3784
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     regulatory      3805..4442
                     /note="UBQ10; ubiquitin10 promoter"
                     /regulatory_class="promoter"
     misc_difference 3958
                     /replace="g"
                     /label=killed PmlI (CACcTG)
                     /note="killed PmlI (CACcTG)"
     misc_difference 4371
                     /replace="t"
                     /label=killed XbaI (aCTAGA)
                     /note="killed XbaI (aCTAGA)"
     primer_bind     4443..4467
                     /label=pDOE mcs3bF with Bsu36I
                     /note="pDOE mcs3bF with Bsu36I"
     misc_feature    4450..4465
                     /label=Bsu361 linker
                     /note="Bsu361 linker"
     regulatory      4466..4531
                     /label=TMV-Omega enhancer
                     /note="TMV-Omega enhancer"
                     /regulatory_class="enhancer"
     misc_feature    4476..4530
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     misc_feature    4532..4579
                     /gene="X-SFS-CVen210"
                     /label=MCS3 front end
                     /note="MCS3 front end"
     CDS             4580..4603
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             4613..4636
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             4643..4666
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     misc_feature    4679..4768
                     /gene="X-SFS-CVen210"
                     /label=CVenus210 specific sequence
                     /note="CVenus210 specific sequence"
     misc_feature    4769..4787
                     /label=MCS3 back end
                     /note="MCS3 back end"
     terminator      4788..5040
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5050..5066
                     /label=remnant from pFGC5941
                     /note="remnant from pFGC5941"
     primer_bind     complement(5091..5107)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5310..5334
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6635..7261
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             7693..8763
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      8832..9026
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9370..9510
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9696..10284)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10374..11165)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta cctcacttgt
      421 acagctcgtc catgccgaga gtaattccag cagctgtaac aaattccaaa agaaccatgt
      481 gatctctctt ttcattaggg tctttagaca acttagattg agtagacaaa tagtggttat
      541 caggcaaaag aactggtcca tcaccaatag gagtattttg ctgataatga tcagccaatt
      601 gaactccacc atcttcaata ttatgtctaa tcttaaaatt agctttaatt ccattctttt
      661 gtttatcagc agtaatataa acattatctg aaaagtaatt atactccaac ttatgaccca
      721 aaatatttcc atcttcttta aagtcaatac ccttcaactc aattctatta accaaagtat
      781 ctccttcaaa cttcacttca gctctagtct tataatttcc atcgtcctta aagaagatag
      841 ttctctcttg aacatatcct tcaggcatag cagacttaaa gaaatcatgt tgtttcatgt
      901 ggtcagggta tctagcaaaa cattgaactc cccaagacaa agttgtcaca agagtcggcc
      961 aaggaaccgg caactttcca gttgtacaaa taaacttcaa agtgagcttt ccataagtag
     1021 catctccctc accctcacca ctaacagaaa acttatgtcc attaacgtcg ccatccaatt
     1081 caacaagaat aggcacaact ccagtaaaca gctcctcgcc cttgctcact gagaaagatg
     1141 aactgtggaa cacgaagtag atgataagga acaaactgtt gagaagcaac atatacagga
     1201 atatcttcag aatcttaggg ttccgcttac tcattcgcga tttggtgtat cgagattggt
     1261 tatgaaattc agatgctagt gtaatgtatt ggtaatttgg gaagatataa taggaagcaa
     1321 ggctatttat ccatttctga aaaggcgaaa tggcgtcacc gcgagcgtca cgcgcattcc
     1381 gttcttgctg taaagcgttg tttggtacac ttttgactag cgaggcttgg cgtgtcagcg
     1441 tatctattca aaagtcgtta atggctgcgg atcaagaaaa agttggaata gaaacagaat
     1501 acccgcgaaa ttcaggcccg gttgccatgt cctacacgcc gaaataaacg accaaattag
     1561 tagaaaaata aaaactgact cggatactta cgtcacgtct tgcgcactga tttgaaaaat
     1621 ctcagaattc ttgtcgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca
     1681 cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca
     1741 attagccaaa aacaactttg cgtgtaaaca acgctcaata cacctgtcat tttattatta
     1801 gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct
     1861 tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc
     1921 aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc
     1981 cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt
     2041 tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat
     2101 catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt
     2161 tttgtcgaat aattactctt cgatttgtga tttctaacta gatctggtgt tagtttctag
     2221 tttgtgcgat cgaatttgtc gattaatctg agtttttctg attaacaggg agaggacacg
     2281 ctcgagtata agagctctat ttttacaaca attaccaaca acaacaaaca acaaacaaca
     2341 ttacaattac atttacaatt accatggggc gcgccctagg aactagtgga ggatccggag
     2401 gtgggtcagg aatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     2461 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     2521 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
     2581 cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg
     2641 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     2701 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     2761 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     2821 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca
     2881 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     2941 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     3001 ccgacaacca ctacctgagc taccagtcca agctgagcaa ataatctaga cccgggggtt
     3061 aattaaccct agagtcctgc tttaatgaga tatgcgagac gcctatgatc gcatgatatt
     3121 tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct cagatcctta
     3181 ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat ttttatgaat
     3241 aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa tattaaaatg
     3301 aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc gccacaataa
     3361 caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag aaccggtcaa
     3421 acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca ggggctagta
     3481 tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc ggttccccgc
     3541 cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct accgaaagtt
     3601 acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc tgccccgaga
     3661 attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac
     3721 agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg tcgaggctca
     3781 gcaggacctg ctccaagagt atggttgtcg acgagtcagt aataaacggc gtcaaagtgg
     3841 ttgcagccgg cacacacgag tcgtgtttat caactcaaag cacaaatact tttcctcaac
     3901 ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta aacaacgctc aatacacctg
     3961 tcattttatt attagctatt gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg
     4021 tcttttcttc ttcttcttct ataaaacaat acccaaagag ctcttcttct tcacaattca
     4081 gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt
     4141 aaatttctgt gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt
     4201 tcgtatatgt tctttggttt agattctgtt aatcttagat cgaagacgat tttctgggtt
     4261 tgatcgttag atatcatctt aattctcgat tagggtttca tagatatcat ccgatttgtt
     4321 caaataattt gagttttgtc gaataattac tcttcgattt gtgatttcta actagatctg
     4381 gtgttagttt ctagtttgtg cgatcgaatt tgtcgattaa tctgagtttt tctgattaac
     4441 agggacacgc ctcaggtaca gagcatattt ttacaacaat taccaacaac aacaaacaac
     4501 aaacaacatt acaattacat ttacaattac aatggggtcc ctacgtagtc acgtgacgtc
     4561 cggaggttct ggtggagctt ggtctcatcc acaattcgag aagtcaggag gagattataa
     4621 ggatgatgat gataaggctt cttggtcaca tcctcagttt gaaaagggat ctgggtcaga
     4681 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     4741 tctcggcatg gacgagctgt acaagtaagc gatcgccatt taaataggat cgttcaaaca
     4801 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     4861 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     4921 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     4981 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     5041 gttaacgata tgtcgaggct cagcaggacc tgcaggcatg caagcttggc actggccgtc
     5101 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     5161 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     5221 cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca gattgtcgtt
     5281 tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
     5341 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
     5401 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
     5461 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
     5521 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
     5581 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
     5641 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
     5701 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
     5761 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
     5821 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
     5881 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
     5941 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
     6001 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
     6061 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
     6121 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
     6181 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
     6241 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
     6301 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
     6361 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
     6421 agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
     6481 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
     6541 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
     6601 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
     6661 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
     6721 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
     6781 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
     6841 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
     6901 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
     6961 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
     7021 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
     7081 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
     7141 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
     7201 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
     7261 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
     7321 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
     7381 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
     7441 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
     7501 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
     7561 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
     7621 gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc
     7681 cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
     7741 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
     7801 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
     7861 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
     7921 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
     7981 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
     8041 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
     8101 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
     8161 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
     8221 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
     8281 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
     8341 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
     8401 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
     8461 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
     8521 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
     8581 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
     8641 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
     8701 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
     8761 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
     8821 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
     8881 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
     8941 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
     9001 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
     9061 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
     9121 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
     9181 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
     9241 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     9301 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     9361 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     9421 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     9481 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     9541 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9601 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9661 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9721 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9781 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9841 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9901 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9961 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10021 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    10081 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
    10141 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    10201 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    10261 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    10321 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
    10381 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
    10441 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
    10501 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
    10561 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
    10621 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
    10681 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
    10741 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
    10801 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
    10861 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
    10921 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
    10981 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
    11041 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
    11101 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
    11161 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
    11221 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
    11281 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
    11341 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
    11401 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
    11461 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
    11521 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
    11581 gctggctgg
//
