LOCUS       40924_14945       12959 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pDOE-08, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12959)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Significant reduction of BiFC non-specific assembly facilitates in 
            planta assessment of heterotrimeric G-protein interactors
  JOURNAL   Plant J. (2014) In press
  PUBMED    25187041
REFERENCE   2  (bases 1 to 12959)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2014) Department of Biology, The Pennsylvania 
            State University, 208 Mueller Laboratory, University Park, PA 16802,
            USA
REFERENCE   3  (bases 1 to 12959)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12959)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-SEP-2014) Department of Biology, The Pennsylvania State 
            University, 208 Mueller Laboratory, University Park, PA 16802, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    99..104
                     /label=non-unique SacI
                     /note="non-unique SacI"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    complement(373..406)
                     /label=BAR gene remnant
                     /note="BAR gene remnant"
     misc_feature    407..412
                     /label=MCS2 KpnI site
                     /note="MCS2 KpnI site"
     CDS             complement(416..1129)
                     /label=mTurquoise2
                     /note="enhanced monomeric variant of CFP (Goedhart et al., 
                     2012)"
     sig_peptide     complement(1130..1234)
                     /gene="XT-Golgi-mTq2"
                     /note="exclusive localization to the medial Golgi
                     cisternae; from Arabidopsis AtXylT/XYLT 
                     (beta-1,2-xylosyltransferase, At5g55500); characterized in 
                     PubMed ID 12943552; confirmed by Saint-Jore-Dupas et al. 
                     (Plant Cell 2006;18;3182-3200)"
     promoter        complement(1238..1618)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2614..2959
                     /label=CaMV35S(short)
                     /note="Cauliflower mosaic virus 35S promoter (short)"
     misc_feature    2962..2978
                     /label=XhoSac linker
                     /note="XhoSac linker"
     misc_feature    2989..3043
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             3045..3728
                     /codon_start=1
                     /gene="NmVen210-X"
                     /product="NmVenus210-mcs1 fusion protein"
                     /label=NmVen210-X
                     /protein_id="AIN46633.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKGASGGGSGSMGR
                     ALGTS"
     gene            3045..3728
                     /gene="NmVen210-X"
                     /label=NmVen210-X
     misc_feature    3045..3674
                     /gene="NmVen210-X"
                     /label=NmVenus210 specific sequence
                     /note="NmVenus210 specific sequence"
     CDS             3045..3563
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     misc_difference 3663..3665
                     /gene="NmVen210-X"
                     /label=A206K monomerizing mutation
                     /note="A206K monomerizing mutation"
     misc_feature    3675..3728
                     /label=MCS1 front end
                     /note="MCS1 front end"
     misc_feature    3729..3750
                     /label=MCS1 back end
                     /note="MCS1 back end"
     terminator      3761..4468
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    4477..4482
                     /label=non-unique NdeI
                     /note="non-unique NdeI"
     promoter        5469..5814
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5810..5834
                     /label=pDOE mcs3bF with Bsu36I
                     /note="pDOE mcs3bF with Bsu36I"
     misc_feature    5817..5832
                     /label=Bsu361 linker
                     /note="Bsu361 linker"
     regulatory      5833..5898
                     /label=TMV-Omega enhancer
                     /note="TMV-Omega enhancer"
                     /regulatory_class="enhancer"
     misc_feature    5843..5897
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     misc_feature    5902..5988
                     /gene="CVen210-SFS-X"
                     /label=CVenus210 specific sequence
                     /note="CVenus210 specific sequence"
     CDS             6001..6024
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             6034..6057
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             6064..6087
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     misc_feature    6103..6138
                     /gene="CVen210-SFS-X"
                     /label=MCS3 front end
                     /note="MCS3 front end"
     misc_feature    6139..6156
                     /label=MCS3 back end
                     /note="MCS3 back end"
     terminator      6158..6410
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6420..6436
                     /label=remnant from pFGC5941
                     /note="remnant from pFGC5941"
     primer_bind     complement(6461..6477)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6680..6704
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8005..8631
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             9063..10133
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      10202..10396
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    10740..10880
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(11066..11654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11744..12535)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta cctcacttgt
      421 acagctcgtc catgccgaga gtaattccag cagctgtaac aaattccaaa agaaccatgt
      481 gatctctctt ttcattaggg tctttagaca acttagattg agtagacaaa tagtggttat
      541 caggcaaaag aactggtcca tcaccaatag gagtattttg ctgataatga tcagccaatt
      601 gaactccacc atcttcaata ttatgtctaa tcttaaaatt agctttaatt ccattctttt
      661 gtttatcagc agtaatataa acattatctg aaaagtaatt atactccaac ttatgaccca
      721 aaatatttcc atcttcttta aagtcaatac ccttcaactc aattctatta accaaagtat
      781 ctccttcaaa cttcacttca gctctagtct tataatttcc atcgtcctta aagaagatag
      841 ttctctcttg aacatatcct tcaggcatag cagacttaaa gaaatcatgt tgtttcatgt
      901 ggtcagggta tctagcaaaa cattgaactc cccaagacaa agttgtcaca agagtcggcc
      961 aaggaaccgg caactttcca gttgtacaaa taaacttcaa agtgagcttt ccataagtag
     1021 catctccctc accctcacca ctaacagaaa acttatgtcc attaacgtcg ccatccaatt
     1081 caacaagaat aggcacaact ccagtaaaca gctcctcgcc cttgctcact gagaaagatg
     1141 aactgtggaa cacgaagtag atgataagga acaaactgtt gagaagcaac atatacagga
     1201 atatcttcag aatcttaggg ttccgcttac tcattcgcga tttggtgtat cgagattggt
     1261 tatgaaattc agatgctagt gtaatgtatt ggtaatttgg gaagatataa taggaagcaa
     1321 ggctatttat ccatttctga aaaggcgaaa tggcgtcacc gcgagcgtca cgcgcattcc
     1381 gttcttgctg taaagcgttg tttggtacac ttttgactag cgaggcttgg cgtgtcagcg
     1441 tatctattca aaagtcgtta atggctgcgg atcaagaaaa agttggaata gaaacagaat
     1501 acccgcgaaa ttcaggcccg gttgccatgt cctacacgcc gaaataaacg accaaattag
     1561 tagaaaaata aaaactgact cggatactta cgtcacgtct tgcgcactga tttgaaaaat
     1621 ctcagaattc caatcccaca aaaatctgag cttaacagca cagttgctcc tctcagagca
     1681 gaatcgggta ttcaacaccc tcatatcaac tactacgttg tgtataacgg tccacatgcc
     1741 ggtatatacg atgactgggg ttgtacaaag gcggcaacaa acggcgttcc cggagttgca
     1801 cacaagaaat ttgccactat tacagaggca agagcagcag ctgacgcgta cacaacaagt
     1861 cagcaaacag acaggttgaa cttcatcccc aaaggagaag ctcaactcaa gcccaagagc
     1921 tttgctaagg ccctaacaag cccaccaaag caaaaagccc actggctcac gctaggaacc
     1981 aaaaggccca gcagtgatcc agccccaaaa gagatctcct ttgccccgga gattacaatg
     2041 gacgatttcc tctatcttta cgatctagga aggaagttcg aaggtgaagg tgacgacact
     2101 atgttcacca ctgataatga gaaggttagc ctcttcaatt tcagaaagaa tgctgaccca
     2161 cagatggtta gagaggccta cgcagcaggt ctcatcaaga cgatctaccc gagtaacaat
     2221 ctccaggaga tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact
     2281 aattgcatca agaacacaga gaaagacata tttctcaaga tcagaagtac tattccagta
     2341 tggacgattc aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaaa
     2401 aaggtagttc ctactgaatc taaggccatg catggagtct aagattcaaa tcgaggatct
     2461 aacagaactc gccgtgaaga ctggcgaaca gttcatacag agtcttttac gactcaatga
     2521 caagaagaaa atcttcgtca acatggtgga gcacgacact ctggtctact ccaaaaatgt
     2581 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa ggataatttc
     2641 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
     2701 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
     2761 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
     2821 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgacatct ccactgacgt
     2881 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
     2941 atttcatttg gagaggacac gctcgagtat aagagctcta tttttacaac aattaccaac
     3001 aacaacaaac aacaaacaac attacaatta catttacaat tacaatggtg agcaagggcg
     3061 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     3121 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     3181 agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg
     3241 gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     3301 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     3361 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     3421 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     3481 acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
     3541 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga
     3601 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     3661 ccaagctgag caaaggagcc agtggaggtg ggtcaggatc catggggcgc gccctaggaa
     3721 ctagttaatc tagacccggg ggttaattaa ccctagagtc ctgctttaat gagatatgcg
     3781 agacgcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg taaaaaacct
     3841 gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga atatatcacc
     3901 cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt gtaccctact
     3961 acttatatgt acaatattaa aatgaaaaca atatattgtg ctgaataggt ttatagcgac
     4021 atctatgata gagcgccaca ataacaaaca attgcgtttt attattacaa atccaatttt
     4081 aaaaaaagcg gcagaaccgg tcaaacctaa aagactgatt acataaatct tattcaaatt
     4141 tcaaaagtgc cccaggggct agtatctacg acacaccgag cggcgaacta ataacgctca
     4201 ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga gattccttga agttgagtat
     4261 tggccgtccg ctctaccgaa agttacgggc accattcaac ccggtccagc acggcggccg
     4321 ggtaaccgac ttgctgcccc gagaattatg cagcattttt ttggtgtatg tgggccccaa
     4381 atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat
     4441 tttgcgacaa catgtcgagg ctcagcagga cctgctcata tgttccaatc ccacaaaaat
     4501 ctgagcttaa cagcacagtt gctcctctca gagcagaatc gggtattcaa caccctcata
     4561 tcaactacta cgttgtgtat aacggtccac atgccggtat atacgatgac tggggttgta
     4621 caaaggcggc aacaaacggc gttcccggag ttgcacacaa gaaatttgcc actattacag
     4681 aggcaagagc agcagctgac gcgtacacaa caagtcagca aacagacagg ttgaacttca
     4741 tccccaaagg agaagctcaa ctcaagccca agagctttgc taaggcccta acaagcccac
     4801 caaagcaaaa agcccactgg ctcacgctag gaaccaaaag gcccagcagt gatccagccc
     4861 caaaagagat ctcctttgcc ccggagatta caatggacga tttcctctat ctttacgatc
     4921 taggaaggaa gttcgaaggt gaaggtgacg acactatgtt caccactgat aatgagaagg
     4981 ttagcctctt caatttcaga aagaatgctg acccacagat ggttagagag gcctacgcag
     5041 caggtctcat caagacgatc tacccgagta acaatctcca ggagatcaaa taccttccca
     5101 agaaggttaa agatgcagtc aaaagattca ggactaattg catcaagaac acagagaaag
     5161 acatatttct caagatcaga agtactattc cagtatggac gattcaaggc ttgcttcata
     5221 aaccaaggca agtaatagag attggagtct ctaaaaaggt agttcctact gaatctaagg
     5281 ccatgcatgg agtctaagat tcaaatcgag gatctaacag aactcgccgt gaagactggc
     5341 gaacagttca tacagagtct tttacgactc aatgacaaga agaaaatctt cgtcaacatg
     5401 gtggagcacg acactctggt ctactccaaa aatgtcaaag atacagtctc agaagaccaa
     5461 agggctattg agacttttca acaaaggata atttcgggaa acctcctcgg attccattgc
     5521 ccagctatct gtcacttcat cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc
     5581 catcattgcg ataaaggaaa ggctatcatt caagatctct ctgccgacag tggtcccaaa
     5641 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     5701 aagcaagtgg attgatgtga catctccact gacgtaaggg atgacgcaca atcccactat
     5761 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag gacacgcctc
     5821 aggtacagag catattttta caacaattac caacaacaac aaacaacaaa caacattaca
     5881 attacattta caattacaat ggaccccaac gagaagcgcg atcacatggt cctgctggag
     5941 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg atctgggtca
     6001 tggtctcatc cacaattcga gaagtcagga ggagattata aggatgatga tgataaggct
     6061 tcttggtcac atcctcagtt tgaaaagtct ggagcttcag gaatggggtc cctacgtagt
     6121 cacgtgacgt ccggataagc gatcgccatt taaataggat cgttcaaaca tttggcaata
     6181 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     6241 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     6301 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     6361 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gttaacgata
     6421 tgtcgaggct cagcaggacc tgcaggcatg caagcttggc actggccgtc gttttacaac
     6481 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     6541 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     6601 gcctgaatgg cgaatgctag agcagcttga gcttggatca gattgtcgtt tcccgccttc
     6661 agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
     6721 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     6781 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
     6841 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
     6901 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
     6961 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
     7021 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
     7081 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
     7141 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
     7201 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
     7261 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
     7321 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     7381 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
     7441 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     7501 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     7561 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     7621 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     7681 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     7741 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     7801 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     7861 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     7921 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
     7981 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     8041 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     8101 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     8161 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     8221 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     8281 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     8341 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     8401 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     8461 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     8521 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     8581 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     8641 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     8701 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     8761 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     8821 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
     8881 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     8941 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     9001 agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     9061 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
     9121 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
     9181 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
     9241 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
     9301 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     9361 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     9421 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     9481 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     9541 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     9601 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     9661 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     9721 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     9781 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
     9841 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     9901 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     9961 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
    10021 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
    10081 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
    10141 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
    10201 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
    10261 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
    10321 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
    10381 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
    10441 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
    10501 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
    10561 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
    10621 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
    10681 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
    10741 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
    10801 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
    10861 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    10921 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    10981 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    11041 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    11101 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    11161 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    11221 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    11281 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    11341 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    11401 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    11461 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    11521 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    11581 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    11641 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    11701 aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta
    11761 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct
    11821 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat
    11881 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt
    11941 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct
    12001 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc
    12061 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc
    12121 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga
    12181 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg
    12241 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa
    12301 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc
    12361 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt
    12421 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc
    12481 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt
    12541 atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac
    12601 gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc
    12661 aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg
    12721 gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat
    12781 catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg
    12841 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
    12901 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctgg
//
