LOCUS       40924_14935       12956 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pDOE-06, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12956)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Significant reduction of BiFC non-specific assembly facilitates in 
            planta assessment of heterotrimeric G-protein interactors
  JOURNAL   Plant J. (2014) In press
  PUBMED    25187041
REFERENCE   2  (bases 1 to 12956)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2014) Department of Biology, The Pennsylvania 
            State University, 208 Mueller Laboratory, University Park, PA 16802,
            USA
REFERENCE   3  (bases 1 to 12956)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12956)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-SEP-2014) Department of Biology, The Pennsylvania State 
            University, 208 Mueller Laboratory, University Park, PA 16802, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    99..104
                     /label=non-unique SacI
                     /note="non-unique SacI"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    complement(373..406)
                     /label=BAR gene remnant
                     /note="BAR gene remnant"
     misc_feature    407..412
                     /label=MCS2 KpnI site
                     /note="MCS2 KpnI site"
     CDS             complement(416..1129)
                     /label=mTurquoise2
                     /note="enhanced monomeric variant of CFP (Goedhart et al., 
                     2012)"
     sig_peptide     complement(1130..1234)
                     /gene="XT-Golgi-mTq2"
                     /note="exclusive localization to the medial Golgi
                     cisternae; from Arabidopsis AtXylT/XYLT 
                     (beta-1,2-xylosyltransferase, At5g55500); characterized in 
                     PubMed ID 12943552; confirmed by Saint-Jore-Dupas et al. 
                     (Plant Cell 2006;18;3182-3200)"
     promoter        complement(1238..1618)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2614..2959
                     /label=CaMV35S(short)
                     /note="Cauliflower mosaic virus 35S promoter (short)"
     misc_feature    2962..2978
                     /label=XhoSac linker
                     /note="XhoSac linker"
     misc_feature    2989..3043
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             3045..3725
                     /codon_start=1
                     /gene="X-NmVen210"
                     /product="mcs1-NmVenus210 fusion protein"
                     /label=X-NmVen210
                     /protein_id="AIN46625.1"
                     /translation="MGRALGTSGGSGGGSGMVSKGEELFTGVVPILVELDGDVNGHKFS
                     VSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSA
                     MPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYN
                     SHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQS
                     KLSK"
     gene            3045..3725
                     /gene="X-NmVen210"
                     /label=X-NmVen210
     misc_feature    3045..3092
                     /label=MCS1 front end
                     /note="MCS1 front end"
     misc_feature    3093..3725
                     /gene="X-NmVen210"
                     /label=NmVenus210 specific sequence
                     /note="NmVenus210 specific sequence"
     CDS             3093..3611
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     misc_difference 3711..3713
                     /gene="X-NmVen210"
                     /label=A206K monomerizing mutation
                     /note="A206K monomerizing mutation"
     misc_feature    3726..3747
                     /label=MCS1 back end
                     /note="MCS1 back end"
     terminator      3758..4465
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    4474..4479
                     /label=non-unique NdeI
                     /note="non-unique NdeI"
     promoter        5466..5811
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5807..5831
                     /label=pDOE mcs3bF with Bsu36I
                     /note="pDOE mcs3bF with Bsu36I"
     misc_feature    5814..5829
                     /label=Bsu361 linker
                     /note="Bsu361 linker"
     regulatory      5830..5895
                     /label=TMV-Omega enhancer
                     /note="TMV-Omega enhancer"
                     /regulatory_class="enhancer"
     misc_feature    5840..5894
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     misc_feature    5899..5985
                     /gene="CVen210-SFS-X"
                     /label=CVenus210 specific sequence
                     /note="CVenus210 specific sequence"
     CDS             5998..6021
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             6031..6054
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             6061..6084
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     misc_feature    6100..6135
                     /label=MCS3 front end
                     /note="MCS3 front end"
     misc_feature    6136..6153
                     /label=MCS3 back end
                     /note="MCS3 back end"
     terminator      6155..6407
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6417..6433
                     /label=remnant from pFGC5941
                     /note="remnant from pFGC5941"
     primer_bind     complement(6458..6474)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6677..6701
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8002..8628
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             9060..10130
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      10199..10393
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    10737..10877
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(11063..11651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11741..12532)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta cctcacttgt
      421 acagctcgtc catgccgaga gtaattccag cagctgtaac aaattccaaa agaaccatgt
      481 gatctctctt ttcattaggg tctttagaca acttagattg agtagacaaa tagtggttat
      541 caggcaaaag aactggtcca tcaccaatag gagtattttg ctgataatga tcagccaatt
      601 gaactccacc atcttcaata ttatgtctaa tcttaaaatt agctttaatt ccattctttt
      661 gtttatcagc agtaatataa acattatctg aaaagtaatt atactccaac ttatgaccca
      721 aaatatttcc atcttcttta aagtcaatac ccttcaactc aattctatta accaaagtat
      781 ctccttcaaa cttcacttca gctctagtct tataatttcc atcgtcctta aagaagatag
      841 ttctctcttg aacatatcct tcaggcatag cagacttaaa gaaatcatgt tgtttcatgt
      901 ggtcagggta tctagcaaaa cattgaactc cccaagacaa agttgtcaca agagtcggcc
      961 aaggaaccgg caactttcca gttgtacaaa taaacttcaa agtgagcttt ccataagtag
     1021 catctccctc accctcacca ctaacagaaa acttatgtcc attaacgtcg ccatccaatt
     1081 caacaagaat aggcacaact ccagtaaaca gctcctcgcc cttgctcact gagaaagatg
     1141 aactgtggaa cacgaagtag atgataagga acaaactgtt gagaagcaac atatacagga
     1201 atatcttcag aatcttaggg ttccgcttac tcattcgcga tttggtgtat cgagattggt
     1261 tatgaaattc agatgctagt gtaatgtatt ggtaatttgg gaagatataa taggaagcaa
     1321 ggctatttat ccatttctga aaaggcgaaa tggcgtcacc gcgagcgtca cgcgcattcc
     1381 gttcttgctg taaagcgttg tttggtacac ttttgactag cgaggcttgg cgtgtcagcg
     1441 tatctattca aaagtcgtta atggctgcgg atcaagaaaa agttggaata gaaacagaat
     1501 acccgcgaaa ttcaggcccg gttgccatgt cctacacgcc gaaataaacg accaaattag
     1561 tagaaaaata aaaactgact cggatactta cgtcacgtct tgcgcactga tttgaaaaat
     1621 ctcagaattc caatcccaca aaaatctgag cttaacagca cagttgctcc tctcagagca
     1681 gaatcgggta ttcaacaccc tcatatcaac tactacgttg tgtataacgg tccacatgcc
     1741 ggtatatacg atgactgggg ttgtacaaag gcggcaacaa acggcgttcc cggagttgca
     1801 cacaagaaat ttgccactat tacagaggca agagcagcag ctgacgcgta cacaacaagt
     1861 cagcaaacag acaggttgaa cttcatcccc aaaggagaag ctcaactcaa gcccaagagc
     1921 tttgctaagg ccctaacaag cccaccaaag caaaaagccc actggctcac gctaggaacc
     1981 aaaaggccca gcagtgatcc agccccaaaa gagatctcct ttgccccgga gattacaatg
     2041 gacgatttcc tctatcttta cgatctagga aggaagttcg aaggtgaagg tgacgacact
     2101 atgttcacca ctgataatga gaaggttagc ctcttcaatt tcagaaagaa tgctgaccca
     2161 cagatggtta gagaggccta cgcagcaggt ctcatcaaga cgatctaccc gagtaacaat
     2221 ctccaggaga tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact
     2281 aattgcatca agaacacaga gaaagacata tttctcaaga tcagaagtac tattccagta
     2341 tggacgattc aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaaa
     2401 aaggtagttc ctactgaatc taaggccatg catggagtct aagattcaaa tcgaggatct
     2461 aacagaactc gccgtgaaga ctggcgaaca gttcatacag agtcttttac gactcaatga
     2521 caagaagaaa atcttcgtca acatggtgga gcacgacact ctggtctact ccaaaaatgt
     2581 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa ggataatttc
     2641 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
     2701 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
     2761 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
     2821 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgacatct ccactgacgt
     2881 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
     2941 atttcatttg gagaggacac gctcgagtat aagagctcta tttttacaac aattaccaac
     3001 aacaacaaac aacaaacaac attacaatta catttacaat taccatgggg cgcgccctag
     3061 gaactagtgg aggatccgga ggtgggtcag gaatggtgag caagggcgag gagctgttca
     3121 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3181 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca
     3241 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc
     3301 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3361 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3421 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3481 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     3541 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
     3601 acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     3661 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc aagctgagca
     3721 aataatctag acccgggggt taattaaccc tagagtcctg ctttaatgag atatgcgaga
     3781 cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
     3841 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     3901 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgta ccctactact
     3961 tatatgtaca atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc
     4021 tatgatagag cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa
     4081 aaaagcggca gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca
     4141 aaagtgcccc aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg
     4201 aagggaactc cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg
     4261 ccgtccgctc taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt
     4321 aaccgacttg ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg
     4381 aagtgcaggt caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt
     4441 gcgacaacat gtcgaggctc agcaggacct gctcatatgt tccaatccca caaaaatctg
     4501 agcttaacag cacagttgct cctctcagag cagaatcggg tattcaacac cctcatatca
     4561 actactacgt tgtgtataac ggtccacatg ccggtatata cgatgactgg ggttgtacaa
     4621 aggcggcaac aaacggcgtt cccggagttg cacacaagaa atttgccact attacagagg
     4681 caagagcagc agctgacgcg tacacaacaa gtcagcaaac agacaggttg aacttcatcc
     4741 ccaaaggaga agctcaactc aagcccaaga gctttgctaa ggccctaaca agcccaccaa
     4801 agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc cagcagtgat ccagccccaa
     4861 aagagatctc ctttgccccg gagattacaa tggacgattt cctctatctt tacgatctag
     4921 gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac cactgataat gagaaggtta
     4981 gcctcttcaa tttcagaaag aatgctgacc cacagatggt tagagaggcc tacgcagcag
     5041 gtctcatcaa gacgatctac ccgagtaaca atctccagga gatcaaatac cttcccaaga
     5101 aggttaaaga tgcagtcaaa agattcagga ctaattgcat caagaacaca gagaaagaca
     5161 tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg cttcataaac
     5221 caaggcaagt aatagagatt ggagtctcta aaaaggtagt tcctactgaa tctaaggcca
     5281 tgcatggagt ctaagattca aatcgaggat ctaacagaac tcgccgtgaa gactggcgaa
     5341 cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt caacatggtg
     5401 gagcacgaca ctctggtcta ctccaaaaat gtcaaagata cagtctcaga agaccaaagg
     5461 gctattgaga cttttcaaca aaggataatt tcgggaaacc tcctcggatt ccattgccca
     5521 gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat
     5581 cattgcgata aaggaaaggc tatcattcaa gatctctctg ccgacagtgg tcccaaagat
     5641 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     5701 caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc ccactatcct
     5761 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgcctcagg
     5821 tacagagcat atttttacaa caattaccaa caacaacaaa caacaaacaa cattacaatt
     5881 acatttacaa ttacaatgga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     5941 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggatc tgggtcatgg
     6001 tctcatccac aattcgagaa gtcaggagga gattataagg atgatgatga taaggcttct
     6061 tggtcacatc ctcagtttga aaagtctgga gcttcaggaa tggggtccct acgtagtcac
     6121 gtgacgtccg gataagcgat cgccatttaa ataggatcgt tcaaacattt ggcaataaag
     6181 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     6241 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     6301 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     6361 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcgtt aacgatatgt
     6421 cgaggctcag caggacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc
     6481 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     6541 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     6601 tgaatggcga atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt
     6661 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     6721 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     6781 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct
     6841 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc
     6901 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg
     6961 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga
     7021 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact
     7081 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga
     7141 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg
     7201 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg
     7261 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg
     7321 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt
     7381 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag
     7441 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc
     7501 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct
     7561 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc
     7621 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg
     7681 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat
     7741 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca
     7801 cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc
     7861 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt
     7921 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa
     7981 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca
     8041 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt
     8101 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat
     8161 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc
     8221 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc
     8281 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
     8341 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
     8401 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
     8461 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
     8521 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
     8581 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
     8641 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
     8701 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
     8761 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
     8821 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa
     8881 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
     8941 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
     9001 ggctgggttg cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
     9061 tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
     9121 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
     9181 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
     9241 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
     9301 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
     9361 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
     9421 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg
     9481 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
     9541 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
     9601 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
     9661 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
     9721 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
     9781 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
     9841 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
     9901 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
     9961 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
    10021 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
    10081 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
    10141 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg
    10201 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
    10261 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
    10321 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
    10381 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
    10441 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
    10501 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
    10561 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac
    10621 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
    10681 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
    10741 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
    10801 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
    10861 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    10921 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    10981 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    11041 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    11101 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    11161 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    11221 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    11281 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    11341 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    11401 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    11461 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
    11521 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    11581 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    11641 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    11701 actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa
    11761 tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga
    11821 catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc
    11881 acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca
    11941 caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt
    12001 ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt
    12061 tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc
    12121 tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc
    12181 actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc
    12241 aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt
    12301 gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt
    12361 ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat
    12421 tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc
    12481 ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt
    12541 tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa
    12601 ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa
    12661 gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg
    12721 atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat
    12781 ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc
    12841 aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct
    12901 gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctgg
//
