LOCUS       40924_14930       12953 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pDOE-05, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12953)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Significant reduction of BiFC non-specific assembly facilitates in 
            planta assessment of heterotrimeric G-protein interactors
  JOURNAL   Plant J. (2014) In press
  PUBMED    25187041
REFERENCE   2  (bases 1 to 12953)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2014) Department of Biology, The Pennsylvania 
            State University, 208 Mueller Laboratory, University Park, PA 16802,
            USA
REFERENCE   3  (bases 1 to 12953)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12953)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-SEP-2014) Department of Biology, The Pennsylvania State 
            University, 208 Mueller Laboratory, University Park, PA 16802, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    99..104
                     /label=non-unique SacI
                     /note="non-unique SacI"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    complement(373..406)
                     /label=BAR gene remnant
                     /note="BAR gene remnant"
     misc_feature    407..412
                     /label=MCS2 KpnI site
                     /note="MCS2 KpnI site"
     CDS             complement(416..1129)
                     /label=mTurquoise2
                     /note="enhanced monomeric variant of CFP (Goedhart et al., 
                     2012)"
     sig_peptide     complement(1130..1234)
                     /gene="XT-Golgi-mTq2"
                     /note="exclusive localization to the medial Golgi
                     cisternae; from Arabidopsis AtXylT/XYLT 
                     (beta-1,2-xylosyltransferase, At5g55500); characterized in 
                     PubMed ID 12943552; confirmed by Saint-Jore-Dupas et al. 
                     (Plant Cell 2006;18;3182-3200)"
     promoter        complement(1238..1618)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2614..2959
                     /label=CaMV35S(short)
                     /note="Cauliflower mosaic virus 35S promoter (short)"
     misc_feature    2962..2978
                     /label=XhoSac linker
                     /note="XhoSac linker"
     misc_feature    2989..3043
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             3045..3725
                     /codon_start=1
                     /gene="X-NmVen210"
                     /product="mcs1-NmVenus210 fusion protein"
                     /label=X-NmVen210
                     /protein_id="AIN46621.1"
                     /translation="MGRALGTSGGSGGGSGMVSKGEELFTGVVPILVELDGDVNGHKFS
                     VSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSA
                     MPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYN
                     SHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQS
                     KLSK"
     gene            3045..3725
                     /gene="X-NmVen210"
                     /label=X-NmVen210
     misc_feature    3045..3092
                     /label=MCS1 front end
                     /note="MCS1 front end"
     misc_feature    3093..3725
                     /gene="X-NmVen210"
                     /label=NmVenus210 specific sequence
                     /note="NmVenus210 specific sequence"
     CDS             3093..3611
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     misc_difference 3711..3713
                     /gene="X-NmVen210"
                     /label=A206K monomerizing mutation
                     /note="A206K monomerizing mutation"
     misc_feature    3726..3747
                     /label=MCS1 back end
                     /note="MCS1 back end"
     terminator      3758..4465
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    4474..4479
                     /label=non-unique NdeI
                     /note="non-unique NdeI"
     promoter        5466..5811
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5807..5831
                     /label=pDOE mcs3bF with Bsu36I
                     /note="pDOE mcs3bF with Bsu36I"
     misc_feature    5814..5829
                     /label=Bsu361 linker
                     /note="Bsu361 linker"
     regulatory      5830..5895
                     /label=TMV-Omega enhancer
                     /note="TMV-Omega enhancer"
                     /regulatory_class="enhancer"
     misc_feature    5840..5894
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     misc_feature    5896..5943
                     /label=MCS3 front end
                     /note="MCS3 front end"
     CDS             5944..5967
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             5977..6000
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             6007..6030
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     misc_feature    6043..6132
                     /gene="X-SFS-CVen210"
                     /label=CVenus210 specific sequence
                     /note="CVenus210 specific sequence"
     misc_feature    6133..6151
                     /label=MCS3 back end
                     /note="MCS3 back end"
     terminator      6152..6404
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6414..6430
                     /label=remnant from pFGC5941
                     /note="remnant from pFGC5941"
     primer_bind     complement(6455..6471)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6674..6698
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7999..8625
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             9057..10127
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      10196..10390
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    10734..10874
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(11060..11648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11738..12529)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta cctcacttgt
      421 acagctcgtc catgccgaga gtaattccag cagctgtaac aaattccaaa agaaccatgt
      481 gatctctctt ttcattaggg tctttagaca acttagattg agtagacaaa tagtggttat
      541 caggcaaaag aactggtcca tcaccaatag gagtattttg ctgataatga tcagccaatt
      601 gaactccacc atcttcaata ttatgtctaa tcttaaaatt agctttaatt ccattctttt
      661 gtttatcagc agtaatataa acattatctg aaaagtaatt atactccaac ttatgaccca
      721 aaatatttcc atcttcttta aagtcaatac ccttcaactc aattctatta accaaagtat
      781 ctccttcaaa cttcacttca gctctagtct tataatttcc atcgtcctta aagaagatag
      841 ttctctcttg aacatatcct tcaggcatag cagacttaaa gaaatcatgt tgtttcatgt
      901 ggtcagggta tctagcaaaa cattgaactc cccaagacaa agttgtcaca agagtcggcc
      961 aaggaaccgg caactttcca gttgtacaaa taaacttcaa agtgagcttt ccataagtag
     1021 catctccctc accctcacca ctaacagaaa acttatgtcc attaacgtcg ccatccaatt
     1081 caacaagaat aggcacaact ccagtaaaca gctcctcgcc cttgctcact gagaaagatg
     1141 aactgtggaa cacgaagtag atgataagga acaaactgtt gagaagcaac atatacagga
     1201 atatcttcag aatcttaggg ttccgcttac tcattcgcga tttggtgtat cgagattggt
     1261 tatgaaattc agatgctagt gtaatgtatt ggtaatttgg gaagatataa taggaagcaa
     1321 ggctatttat ccatttctga aaaggcgaaa tggcgtcacc gcgagcgtca cgcgcattcc
     1381 gttcttgctg taaagcgttg tttggtacac ttttgactag cgaggcttgg cgtgtcagcg
     1441 tatctattca aaagtcgtta atggctgcgg atcaagaaaa agttggaata gaaacagaat
     1501 acccgcgaaa ttcaggcccg gttgccatgt cctacacgcc gaaataaacg accaaattag
     1561 tagaaaaata aaaactgact cggatactta cgtcacgtct tgcgcactga tttgaaaaat
     1621 ctcagaattc caatcccaca aaaatctgag cttaacagca cagttgctcc tctcagagca
     1681 gaatcgggta ttcaacaccc tcatatcaac tactacgttg tgtataacgg tccacatgcc
     1741 ggtatatacg atgactgggg ttgtacaaag gcggcaacaa acggcgttcc cggagttgca
     1801 cacaagaaat ttgccactat tacagaggca agagcagcag ctgacgcgta cacaacaagt
     1861 cagcaaacag acaggttgaa cttcatcccc aaaggagaag ctcaactcaa gcccaagagc
     1921 tttgctaagg ccctaacaag cccaccaaag caaaaagccc actggctcac gctaggaacc
     1981 aaaaggccca gcagtgatcc agccccaaaa gagatctcct ttgccccgga gattacaatg
     2041 gacgatttcc tctatcttta cgatctagga aggaagttcg aaggtgaagg tgacgacact
     2101 atgttcacca ctgataatga gaaggttagc ctcttcaatt tcagaaagaa tgctgaccca
     2161 cagatggtta gagaggccta cgcagcaggt ctcatcaaga cgatctaccc gagtaacaat
     2221 ctccaggaga tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact
     2281 aattgcatca agaacacaga gaaagacata tttctcaaga tcagaagtac tattccagta
     2341 tggacgattc aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaaa
     2401 aaggtagttc ctactgaatc taaggccatg catggagtct aagattcaaa tcgaggatct
     2461 aacagaactc gccgtgaaga ctggcgaaca gttcatacag agtcttttac gactcaatga
     2521 caagaagaaa atcttcgtca acatggtgga gcacgacact ctggtctact ccaaaaatgt
     2581 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa ggataatttc
     2641 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
     2701 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
     2761 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
     2821 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgacatct ccactgacgt
     2881 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
     2941 atttcatttg gagaggacac gctcgagtat aagagctcta tttttacaac aattaccaac
     3001 aacaacaaac aacaaacaac attacaatta catttacaat taccatgggg cgcgccctag
     3061 gaactagtgg aggatccgga ggtgggtcag gaatggtgag caagggcgag gagctgttca
     3121 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3181 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca
     3241 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc
     3301 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3361 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3421 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3481 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     3541 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
     3601 acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     3661 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc aagctgagca
     3721 aataatctag acccgggggt taattaaccc tagagtcctg ctttaatgag atatgcgaga
     3781 cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
     3841 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     3901 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgta ccctactact
     3961 tatatgtaca atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc
     4021 tatgatagag cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa
     4081 aaaagcggca gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca
     4141 aaagtgcccc aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg
     4201 aagggaactc cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg
     4261 ccgtccgctc taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt
     4321 aaccgacttg ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg
     4381 aagtgcaggt caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt
     4441 gcgacaacat gtcgaggctc agcaggacct gctcatatgt tccaatccca caaaaatctg
     4501 agcttaacag cacagttgct cctctcagag cagaatcggg tattcaacac cctcatatca
     4561 actactacgt tgtgtataac ggtccacatg ccggtatata cgatgactgg ggttgtacaa
     4621 aggcggcaac aaacggcgtt cccggagttg cacacaagaa atttgccact attacagagg
     4681 caagagcagc agctgacgcg tacacaacaa gtcagcaaac agacaggttg aacttcatcc
     4741 ccaaaggaga agctcaactc aagcccaaga gctttgctaa ggccctaaca agcccaccaa
     4801 agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc cagcagtgat ccagccccaa
     4861 aagagatctc ctttgccccg gagattacaa tggacgattt cctctatctt tacgatctag
     4921 gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac cactgataat gagaaggtta
     4981 gcctcttcaa tttcagaaag aatgctgacc cacagatggt tagagaggcc tacgcagcag
     5041 gtctcatcaa gacgatctac ccgagtaaca atctccagga gatcaaatac cttcccaaga
     5101 aggttaaaga tgcagtcaaa agattcagga ctaattgcat caagaacaca gagaaagaca
     5161 tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg cttcataaac
     5221 caaggcaagt aatagagatt ggagtctcta aaaaggtagt tcctactgaa tctaaggcca
     5281 tgcatggagt ctaagattca aatcgaggat ctaacagaac tcgccgtgaa gactggcgaa
     5341 cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt caacatggtg
     5401 gagcacgaca ctctggtcta ctccaaaaat gtcaaagata cagtctcaga agaccaaagg
     5461 gctattgaga cttttcaaca aaggataatt tcgggaaacc tcctcggatt ccattgccca
     5521 gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat
     5581 cattgcgata aaggaaaggc tatcattcaa gatctctctg ccgacagtgg tcccaaagat
     5641 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     5701 caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc ccactatcct
     5761 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgcctcagg
     5821 tacagagcat atttttacaa caattaccaa caacaacaaa caacaaacaa cattacaatt
     5881 acatttacaa ttacaatggg gtccctacgt agtcacgtga cgtccggagg ttctggtgga
     5941 gcttggtctc atccacaatt cgagaagtca ggaggagatt ataaggatga tgatgataag
     6001 gcttcttggt cacatcctca gtttgaaaag ggatctgggt cagaccccaa cgagaagcgc
     6061 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     6121 ctgtacaagt aagcgatcgc catttaaata ggatcgttca aacatttggc aataaagttt
     6181 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     6241 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     6301 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     6361 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgttaac gatatgtcga
     6421 ggctcagcag gacctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg
     6481 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     6541 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     6601 atggcgaatg ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta
     6661 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     6721 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     6781 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
     6841 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
     6901 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
     6961 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
     7021 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
     7081 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
     7141 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
     7201 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
     7261 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
     7321 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
     7381 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
     7441 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
     7501 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
     7561 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
     7621 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
     7681 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
     7741 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     7801 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     7861 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     7921 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     7981 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     8041 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     8101 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     8161 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     8221 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     8281 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     8341 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     8401 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     8461 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     8521 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     8581 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     8641 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     8701 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     8761 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     8821 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     8881 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     8941 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     9001 tgggttgcct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     9061 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
     9121 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
     9181 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
     9241 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
     9301 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
     9361 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
     9421 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
     9481 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
     9541 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
     9601 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
     9661 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
     9721 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
     9781 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
     9841 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
     9901 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
     9961 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
    10021 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
    10081 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
    10141 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg
    10201 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
    10261 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
    10321 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
    10381 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
    10441 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
    10501 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
    10561 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt
    10621 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
    10681 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
    10741 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
    10801 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    10861 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    10921 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    10981 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    11041 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    11101 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    11161 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    11221 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    11281 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    11341 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    11401 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    11461 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    11521 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    11581 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    11641 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    11701 cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat
    11761 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
    11821 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
    11881 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
    11941 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
    12001 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
    12061 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt
    12121 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
    12181 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa
    12241 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
    12301 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
    12361 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt
    12421 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
    12481 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
    12541 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
    12601 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
    12661 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc
    12721 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg
    12781 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa
    12841 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
    12901 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tgg
//
