LOCUS       V007956                12656 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007956
VERSION     V007956
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12656)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Significant reduction of BiFC non-specific assembly facilitates in
            planta assessment of heterotrimeric G-protein interactors
  JOURNAL   Plant J. (2014) In press
   PUBMED   25187041
REFERENCE   2  (bases 1 to 12656)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2014) Department of Biology, The Pennsylvania
            State University, 208 Mueller Laboratory, University Park, PA 16802,
            USA
REFERENCE   3  (bases 1 to 12656)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12656)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.
            (2014) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-SEP-2014) Department of Biology, The Pennsylvania State
            University, 208 Mueller Laboratory, University Park, PA 16802, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    99..104
                     /label="non-unique SacI"
                     /note="non-unique SacI"
     terminator      complement(111..363)
                     /label="MAS terminator"
                     /note="mannopine synthase terminator"
     misc_feature    complement(373..406)
                     /label="BAR gene remnant"
                     /note="BAR gene remnant"
     misc_feature    407..412
                     /label="MCS2 KpnI site"
                     /note="MCS2 KpnI site"
     CDS             complement(416..931)
                     /note="RNA silencing suppressor p19 from Tomato bushy stunt
                     virus (strain Cherry). Accession#: P11690"
                     /label="RNA silencing suppressor p19"
     promoter        complement(935..1315)
                     /label="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2311..2656
                     /label="CaMV35S(short)"
                     /note="Cauliflower mosaic virus 35S promoter (short)"
     misc_feature    2659..2675
                     /label="XhoSac linker"
                     /note="XhoSac linker"
     misc_feature    2686..2740
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2742..3425
                     /codon_start=1
                     /gene="NmVen210-X"
                     /product="NmVenus210-mcs1 fusion protein"
                     /label="NmVen210-X"
                     /protein_id="AIN46617.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKGASGGGSGSMGR
                     ALGTS"
     gene            2742..3425
                     /gene="NmVen210-X"
                     /label="NmVen210-X"
     misc_feature    2742..3371
                     /gene="NmVen210-X"
                     /label="NmVenus210 specific sequence"
                     /note="NmVenus210 specific sequence"
     CDS             2742..3260
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label="VN173"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     misc_difference 3360..3362
                     /gene="NmVen210-X"
                     /label="A206K monomerizing mutation"
                     /note="A206K monomerizing mutation"
     misc_feature    3372..3425
                     /label="MCS1 front end"
                     /note="MCS1 front end"
     misc_feature    3426..3447
                     /label="MCS1 back end"
                     /note="MCS1 back end"
     terminator      3458..4165
                     /label="OCS terminator"
                     /note="octopine synthase terminator"
     misc_feature    4174..4179
                     /label="non-unique NdeI"
                     /note="non-unique NdeI"
     promoter        5166..5511
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5507..5531
                     /label="pDOE mcs3bF with Bsu36I"
                     /note="pDOE mcs3bF with Bsu36I"
     misc_feature    5514..5529
                     /label="Bsu361 linker"
                     /note="Bsu361 linker"
     regulatory      5530..5595
                     /label="TMV-Omega enhancer"
                     /note="TMV-Omega enhancer"
                     /regulatory_class="enhancer"
     misc_feature    5540..5594
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     misc_feature    5599..5685
                     /gene="CVen210-SFS-X"
                     /label="CVenus210 specific sequence"
                     /note="CVenus210 specific sequence"
     CDS             5698..5721
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
     CDS             5731..5754
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             5761..5784
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
     misc_feature    5800..5835
                     /label="MCS3 front end"
                     /note="MCS3 front end"
     misc_feature    5836..5853
                     /label="MCS3 back end"
                     /note="MCS3 back end"
     terminator      5855..6107
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6117..6133
                     /label="remnant from pFGC5941"
                     /note="remnant from pFGC5941"
     primer_bind     complement(6158..6174)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    6377..6401
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7702..8328
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             8760..9830
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      9899..10093
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    10437..10577
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10763..11351)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(11441..12232)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta cctcactctg
      421 actccttctc gaaggtttga gtaccttcag gacatcctct tgaaacatta gattcaactt
      481 caattggagc caactgcaaa agttcttgct tagacctaat tgccatctca cacaaatgtt
      541 gaagagttct tgatcctcca gaaacagtga tactaactcc cctgaaccta atagaataag
      601 tacatccgat ttgatcgaac ccaaagaatc ttgatgcagc atagttgact gaatctccag
      661 tccaagatcc aagcactcta tgcaatgatg cttcagttct atcgtatctg agatacctct
      721 taaacacaac ttttccgaaa ccccaagatt ccttgaaacc aagtggatta tcttgatttg
      781 agttcgtctc atcgttatgc aatctccact cagtccaaga tggactttca tcaggaagtt
      841 tgaaaggaga agttgtacct cctgatcctc catcccatct ttcagagtta gcttgttctc
      901 tagcatcatt tccttgtata gctcgttcca ttcgcgattt ggtgtatcga gattggttat
      961 gaaattcaga tgctagtgta atgtattggt aatttgggaa gatataatag gaagcaaggc
     1021 tatttatcca tttctgaaaa ggcgaaatgg cgtcaccgcg agcgtcacgc gcattccgtt
     1081 cttgctgtaa agcgttgttt ggtacacttt tgactagcga ggcttggcgt gtcagcgtat
     1141 ctattcaaaa gtcgttaatg gctgcggatc aagaaaaagt tggaatagaa acagaatacc
     1201 cgcgaaattc aggcccggtt gccatgtcct acacgccgaa ataaacgacc aaattagtag
     1261 aaaaataaaa actgactcgg atacttacgt cacgtcttgc gcactgattt gaaaaatctc
     1321 agaattccaa tcccacaaaa atctgagctt aacagcacag ttgctcctct cagagcagaa
     1381 tcgggtattc aacaccctca tatcaactac tacgttgtgt ataacggtcc acatgccggt
     1441 atatacgatg actggggttg tacaaaggcg gcaacaaacg gcgttcccgg agttgcacac
     1501 aagaaatttg ccactattac agaggcaaga gcagcagctg acgcgtacac aacaagtcag
     1561 caaacagaca ggttgaactt catccccaaa ggagaagctc aactcaagcc caagagcttt
     1621 gctaaggccc taacaagccc accaaagcaa aaagcccact ggctcacgct aggaaccaaa
     1681 aggcccagca gtgatccagc cccaaaagag atctcctttg ccccggagat tacaatggac
     1741 gatttcctct atctttacga tctaggaagg aagttcgaag gtgaaggtga cgacactatg
     1801 ttcaccactg ataatgagaa ggttagcctc ttcaatttca gaaagaatgc tgacccacag
     1861 atggttagag aggcctacgc agcaggtctc atcaagacga tctacccgag taacaatctc
     1921 caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt caggactaat
     1981 tgcatcaaga acacagagaa agacatattt ctcaagatca gaagtactat tccagtatgg
     2041 acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt ctctaaaaag
     2101 gtagttccta ctgaatctaa ggccatgcat ggagtctaag attcaaatcg aggatctaac
     2161 agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa
     2221 gaagaaaatc ttcgtcaaca tggtggagca cgacactctg gtctactcca aaaatgtcaa
     2281 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaagga taatttcggg
     2341 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtagaaaa
     2401 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca ttcaagatct
     2461 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     2521 agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca ctgacgtaag
     2581 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     2641 tcatttggag aggacacgct cgagtataag agctctattt ttacaacaat taccaacaac
     2701 aacaaacaac aaacaacatt acaattacat ttacaattac aatggtgagc aagggcgagg
     2761 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     2821 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc
     2881 tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct
     2941 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3001 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     3061 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     3121 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     3181 acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
     3241 agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca
     3301 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca
     3361 agctgagcaa aggagccagt ggaggtgggt caggatccat ggggcgcgcc ctaggaacta
     3421 gttaatctag acccgggggt taattaaccc tagagtcctg ctttaatgag atatgcgaga
     3481 cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
     3541 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     3601 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgta ccctactact
     3661 tatatgtaca atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc
     3721 tatgatagag cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa
     3781 aaaagcggca gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca
     3841 aaagtgcccc aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg
     3901 aagggaactc cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg
     3961 ccgtccgctc taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt
     4021 aaccgacttg ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg
     4081 aagtgcaggt caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt
     4141 gcgacaacat gtcgaggctc agcaggacct gctcatatgt tccaatccca caaaaatctg
     4201 agcttaacag cacagttgct cctctcagag cagaatcggg tattcaacac cctcatatca
     4261 actactacgt tgtgtataac ggtccacatg ccggtatata cgatgactgg ggttgtacaa
     4321 aggcggcaac aaacggcgtt cccggagttg cacacaagaa atttgccact attacagagg
     4381 caagagcagc agctgacgcg tacacaacaa gtcagcaaac agacaggttg aacttcatcc
     4441 ccaaaggaga agctcaactc aagcccaaga gctttgctaa ggccctaaca agcccaccaa
     4501 agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc cagcagtgat ccagccccaa
     4561 aagagatctc ctttgccccg gagattacaa tggacgattt cctctatctt tacgatctag
     4621 gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac cactgataat gagaaggtta
     4681 gcctcttcaa tttcagaaag aatgctgacc cacagatggt tagagaggcc tacgcagcag
     4741 gtctcatcaa gacgatctac ccgagtaaca atctccagga gatcaaatac cttcccaaga
     4801 aggttaaaga tgcagtcaaa agattcagga ctaattgcat caagaacaca gagaaagaca
     4861 tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg cttcataaac
     4921 caaggcaagt aatagagatt ggagtctcta aaaaggtagt tcctactgaa tctaaggcca
     4981 tgcatggagt ctaagattca aatcgaggat ctaacagaac tcgccgtgaa gactggcgaa
     5041 cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt caacatggtg
     5101 gagcacgaca ctctggtcta ctccaaaaat gtcaaagata cagtctcaga agaccaaagg
     5161 gctattgaga cttttcaaca aaggataatt tcgggaaacc tcctcggatt ccattgccca
     5221 gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat
     5281 cattgcgata aaggaaaggc tatcattcaa gatctctctg ccgacagtgg tcccaaagat
     5341 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     5401 caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc ccactatcct
     5461 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgcctcagg
     5521 tacagagcat atttttacaa caattaccaa caacaacaaa caacaaacaa cattacaatt
     5581 acatttacaa ttacaatgga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     5641 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggatc tgggtcatgg
     5701 tctcatccac aattcgagaa gtcaggagga gattataagg atgatgatga taaggcttct
     5761 tggtcacatc ctcagtttga aaagtctgga gcttcaggaa tggggtccct acgtagtcac
     5821 gtgacgtccg gataagcgat cgccatttaa ataggatcgt tcaaacattt ggcaataaag
     5881 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     5941 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     6001 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     6061 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcgtt aacgatatgt
     6121 cgaggctcag caggacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc
     6181 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     6241 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     6301 tgaatggcga atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt
     6361 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     6421 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     6481 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct
     6541 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc
     6601 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg
     6661 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga
     6721 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact
     6781 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga
     6841 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg
     6901 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg
     6961 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg
     7021 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt
     7081 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag
     7141 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc
     7201 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct
     7261 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc
     7321 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg
     7381 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat
     7441 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca
     7501 cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc
     7561 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt
     7621 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa
     7681 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca
     7741 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt
     7801 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat
     7861 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc
     7921 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc
     7981 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
     8041 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
     8101 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
     8161 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
     8221 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
     8281 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
     8341 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
     8401 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
     8461 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
     8521 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa
     8581 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
     8641 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
     8701 ggctgggttg cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
     8761 tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
     8821 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
     8881 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
     8941 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
     9001 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
     9061 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
     9121 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg
     9181 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
     9241 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
     9301 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
     9361 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
     9421 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
     9481 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
     9541 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
     9601 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
     9661 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
     9721 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
     9781 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
     9841 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg
     9901 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
     9961 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
    10021 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
    10081 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
    10141 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
    10201 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
    10261 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac
    10321 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
    10381 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
    10441 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
    10501 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
    10561 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    10621 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    10681 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    10741 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    10801 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    10861 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    10921 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    10981 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    11041 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    11101 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    11161 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
    11221 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    11281 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    11341 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    11401 actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa
    11461 tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga
    11521 catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc
    11581 acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca
    11641 caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt
    11701 ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt
    11761 tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc
    11821 tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc
    11881 actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc
    11941 aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt
    12001 gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt
    12061 ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat
    12121 tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc
    12181 ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt
    12241 tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa
    12301 ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa
    12361 gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg
    12421 atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat
    12481 ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc
    12541 aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct
    12601 gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctgg
//
