LOCUS       V007958                12653 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007958
VERSION     V007958
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12653)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Significant reduction of BiFC non-specific assembly facilitates in
            planta assessment of heterotrimeric G-protein interactors
  JOURNAL   Plant J. (2014) In press
   PUBMED   25187041
REFERENCE   2  (bases 1 to 12653)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2014) Department of Biology, The Pennsylvania
            State University, 208 Mueller Laboratory, University Park, PA 16802,
            USA
REFERENCE   3  (bases 1 to 12653)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12653)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.
            (2014) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-SEP-2014) Department of Biology, The Pennsylvania State
            University, 208 Mueller Laboratory, University Park, PA 16802, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    99..104
                     /label="non-unique SacI"
                     /note="non-unique SacI"
     terminator      complement(111..363)
                     /label="MAS terminator"
                     /note="mannopine synthase terminator"
     misc_feature    complement(373..406)
                     /label="BAR gene remnant"
                     /note="BAR gene remnant"
     misc_feature    407..412
                     /label="MCS2 KpnI site"
                     /note="MCS2 KpnI site"
     CDS             complement(416..931)
                     /note="RNA silencing suppressor p19 from Tomato bushy stunt
                     virus (strain Cherry). Accession#: P11690"
                     /label="RNA silencing suppressor p19"
     promoter        complement(935..1315)
                     /label="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2311..2656
                     /label="CaMV35S(short)"
                     /note="Cauliflower mosaic virus 35S promoter (short)"
     misc_feature    2659..2675
                     /label="XhoSac linker"
                     /note="XhoSac linker"
     misc_feature    2686..2740
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2742..3422
                     /codon_start=1
                     /gene="X-NmVen210"
                     /product="mcs1-NmVenus210 fusion protein"
                     /label="X-NmVen210"
                     /protein_id="AIN46609.1"
                     /translation="MGRALGTSGGSGGGSGMVSKGEELFTGVVPILVELDGDVNGHKFS
                     VSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSA
                     MPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYN
                     SHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQS
                     KLSK"
     gene            2742..3422
                     /gene="X-NmVen210"
                     /label="X-NmVen210"
     misc_feature    2742..2789
                     /label="MCS1 front end"
                     /note="MCS1 front end"
     misc_feature    2790..3422
                     /gene="X-NmVen210"
                     /label="NmVenus210 specific sequence"
                     /note="NmVenus210 specific sequence"
     CDS             2790..3308
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label="VN173"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     misc_difference 3408..3410
                     /gene="X-NmVen210"
                     /label="A206K monomerizing mutation"
                     /note="A206K monomerizing mutation"
     misc_feature    3423..3444
                     /label="MCS1 back end"
                     /note="MCS1 back end"
     terminator      3455..4162
                     /label="OCS terminator"
                     /note="octopine synthase terminator"
     misc_feature    4171..4176
                     /label="non-unique NdeI"
                     /note="non-unique NdeI"
     promoter        5163..5508
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5504..5528
                     /label="pDOE mcs3bF with Bsu36I"
                     /note="pDOE mcs3bF with Bsu36I"
     misc_feature    5511..5526
                     /label="Bsu361 linker"
                     /note="Bsu361 linker"
     regulatory      5527..5592
                     /label="TMV-Omega enhancer"
                     /note="TMV-Omega enhancer"
                     /regulatory_class="enhancer"
     misc_feature    5537..5591
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     misc_feature    5596..5682
                     /gene="CVen210-SFS-X"
                     /label="CVenus210 specific sequence"
                     /note="CVenus210 specific sequence"
     CDS             5695..5718
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
     CDS             5728..5751
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             5758..5781
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
     misc_feature    5797..5832
                     /label="MCS3 front end"
                     /note="MCS3 front end"
     misc_feature    5833..5850
                     /label="MCS3 back end"
                     /note="MCS3 back end"
     terminator      5852..6104
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6114..6130
                     /label="remnant from pFGC5941"
                     /note="remnant from pFGC5941"
     primer_bind     complement(6155..6171)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    6374..6398
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7699..8325
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             8757..9827
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      9896..10090
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    10434..10574
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10760..11348)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(11438..12229)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta cctcactctg
      421 actccttctc gaaggtttga gtaccttcag gacatcctct tgaaacatta gattcaactt
      481 caattggagc caactgcaaa agttcttgct tagacctaat tgccatctca cacaaatgtt
      541 gaagagttct tgatcctcca gaaacagtga tactaactcc cctgaaccta atagaataag
      601 tacatccgat ttgatcgaac ccaaagaatc ttgatgcagc atagttgact gaatctccag
      661 tccaagatcc aagcactcta tgcaatgatg cttcagttct atcgtatctg agatacctct
      721 taaacacaac ttttccgaaa ccccaagatt ccttgaaacc aagtggatta tcttgatttg
      781 agttcgtctc atcgttatgc aatctccact cagtccaaga tggactttca tcaggaagtt
      841 tgaaaggaga agttgtacct cctgatcctc catcccatct ttcagagtta gcttgttctc
      901 tagcatcatt tccttgtata gctcgttcca ttcgcgattt ggtgtatcga gattggttat
      961 gaaattcaga tgctagtgta atgtattggt aatttgggaa gatataatag gaagcaaggc
     1021 tatttatcca tttctgaaaa ggcgaaatgg cgtcaccgcg agcgtcacgc gcattccgtt
     1081 cttgctgtaa agcgttgttt ggtacacttt tgactagcga ggcttggcgt gtcagcgtat
     1141 ctattcaaaa gtcgttaatg gctgcggatc aagaaaaagt tggaatagaa acagaatacc
     1201 cgcgaaattc aggcccggtt gccatgtcct acacgccgaa ataaacgacc aaattagtag
     1261 aaaaataaaa actgactcgg atacttacgt cacgtcttgc gcactgattt gaaaaatctc
     1321 agaattccaa tcccacaaaa atctgagctt aacagcacag ttgctcctct cagagcagaa
     1381 tcgggtattc aacaccctca tatcaactac tacgttgtgt ataacggtcc acatgccggt
     1441 atatacgatg actggggttg tacaaaggcg gcaacaaacg gcgttcccgg agttgcacac
     1501 aagaaatttg ccactattac agaggcaaga gcagcagctg acgcgtacac aacaagtcag
     1561 caaacagaca ggttgaactt catccccaaa ggagaagctc aactcaagcc caagagcttt
     1621 gctaaggccc taacaagccc accaaagcaa aaagcccact ggctcacgct aggaaccaaa
     1681 aggcccagca gtgatccagc cccaaaagag atctcctttg ccccggagat tacaatggac
     1741 gatttcctct atctttacga tctaggaagg aagttcgaag gtgaaggtga cgacactatg
     1801 ttcaccactg ataatgagaa ggttagcctc ttcaatttca gaaagaatgc tgacccacag
     1861 atggttagag aggcctacgc agcaggtctc atcaagacga tctacccgag taacaatctc
     1921 caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt caggactaat
     1981 tgcatcaaga acacagagaa agacatattt ctcaagatca gaagtactat tccagtatgg
     2041 acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt ctctaaaaag
     2101 gtagttccta ctgaatctaa ggccatgcat ggagtctaag attcaaatcg aggatctaac
     2161 agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa
     2221 gaagaaaatc ttcgtcaaca tggtggagca cgacactctg gtctactcca aaaatgtcaa
     2281 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaagga taatttcggg
     2341 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtagaaaa
     2401 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca ttcaagatct
     2461 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     2521 agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca ctgacgtaag
     2581 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     2641 tcatttggag aggacacgct cgagtataag agctctattt ttacaacaat taccaacaac
     2701 aacaaacaac aaacaacatt acaattacat ttacaattac catggggcgc gccctaggaa
     2761 ctagtggagg atccggaggt gggtcaggaa tggtgagcaa gggcgaggag ctgttcaccg
     2821 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     2881 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca
     2941 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt
     3001 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     3061 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     3121 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     3181 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     3241 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
     3301 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     3361 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaag ctgagcaaat
     3421 aatctagacc cgggggttaa ttaaccctag agtcctgctt taatgagata tgcgagacgc
     3481 ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa acctgagcat
     3541 gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat cacccgttac
     3601 tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtaccc tactacttat
     3661 atgtacaata ttaaaatgaa aacaatatat tgtgctgaat aggtttatag cgacatctat
     3721 gatagagcgc cacaataaca aacaattgcg ttttattatt acaaatccaa ttttaaaaaa
     3781 agcggcagaa ccggtcaaac ctaaaagact gattacataa atcttattca aatttcaaaa
     3841 gtgccccagg ggctagtatc tacgacacac cgagcggcga actaataacg ctcactgaag
     3901 ggaactccgg ttccccgccg gcgcgcatgg gtgagattcc ttgaagttga gtattggccg
     3961 tccgctctac cgaaagttac gggcaccatt caacccggtc cagcacggcg gccgggtaac
     4021 cgacttgctg ccccgagaat tatgcagcat ttttttggtg tatgtgggcc ccaaatgaag
     4081 tgcaggtcaa accttgacag tgacgacaaa tcgttgggcg ggtccagggc gaattttgcg
     4141 acaacatgtc gaggctcagc aggacctgct catatgttcc aatcccacaa aaatctgagc
     4201 ttaacagcac agttgctcct ctcagagcag aatcgggtat tcaacaccct catatcaact
     4261 actacgttgt gtataacggt ccacatgccg gtatatacga tgactggggt tgtacaaagg
     4321 cggcaacaaa cggcgttccc ggagttgcac acaagaaatt tgccactatt acagaggcaa
     4381 gagcagcagc tgacgcgtac acaacaagtc agcaaacaga caggttgaac ttcatcccca
     4441 aaggagaagc tcaactcaag cccaagagct ttgctaaggc cctaacaagc ccaccaaagc
     4501 aaaaagccca ctggctcacg ctaggaacca aaaggcccag cagtgatcca gccccaaaag
     4561 agatctcctt tgccccggag attacaatgg acgatttcct ctatctttac gatctaggaa
     4621 ggaagttcga aggtgaaggt gacgacacta tgttcaccac tgataatgag aaggttagcc
     4681 tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac gcagcaggtc
     4741 tcatcaagac gatctacccg agtaacaatc tccaggagat caaatacctt cccaagaagg
     4801 ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa gaacacagag aaagacatat
     4861 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa
     4921 ggcaagtaat agagattgga gtctctaaaa aggtagttcc tactgaatct aaggccatgc
     4981 atggagtcta agattcaaat cgaggatcta acagaactcg ccgtgaagac tggcgaacag
     5041 ttcatacaga gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag
     5101 cacgacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga ccaaagggct
     5161 attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct
     5221 atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat
     5281 tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga
     5341 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
     5401 gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     5461 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg cctcaggtac
     5521 agagcatatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattaca
     5581 tttacaatta caatggaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5641 accgccgccg ggatcactct cggcatggac gagctgtaca agggatctgg gtcatggtct
     5701 catccacaat tcgagaagtc aggaggagat tataaggatg atgatgataa ggcttcttgg
     5761 tcacatcctc agtttgaaaa gtctggagct tcaggaatgg ggtccctacg tagtcacgtg
     5821 acgtccggat aagcgatcgc catttaaata ggatcgttca aacatttggc aataaagttt
     5881 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     5941 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     6001 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     6061 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgttaac gatatgtcga
     6121 ggctcagcag gacctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg
     6181 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     6241 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     6301 atggcgaatg ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta
     6361 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     6421 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     6481 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
     6541 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
     6601 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
     6661 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
     6721 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
     6781 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
     6841 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
     6901 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
     6961 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
     7021 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
     7081 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
     7141 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
     7201 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
     7261 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
     7321 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
     7381 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
     7441 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     7501 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     7561 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     7621 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     7681 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     7741 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     7801 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     7861 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     7921 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     7981 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     8041 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     8101 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     8161 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     8221 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     8281 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     8341 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     8401 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     8461 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     8521 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     8581 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     8641 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     8701 tgggttgcct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     8761 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
     8821 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
     8881 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
     8941 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
     9001 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
     9061 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
     9121 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
     9181 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
     9241 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
     9301 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
     9361 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
     9421 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
     9481 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
     9541 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
     9601 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
     9661 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
     9721 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
     9781 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
     9841 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg
     9901 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
     9961 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
    10021 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
    10081 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
    10141 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
    10201 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
    10261 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt
    10321 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
    10381 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
    10441 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
    10501 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    10561 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    10621 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    10681 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    10741 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    10801 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    10861 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    10921 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    10981 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    11041 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    11101 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    11161 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    11221 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    11281 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    11341 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    11401 cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat
    11461 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
    11521 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
    11581 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
    11641 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
    11701 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
    11761 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt
    11821 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
    11881 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa
    11941 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
    12001 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
    12061 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt
    12121 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
    12181 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
    12241 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
    12301 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
    12361 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc
    12421 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg
    12481 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa
    12541 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
    12601 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tgg
//
