LOCUS       V007959                12650 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007959
VERSION     V007959
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12650)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Significant reduction of BiFC non-specific assembly facilitates in
            planta assessment of heterotrimeric G-protein interactors
  JOURNAL   Plant J. (2014) In press
   PUBMED   25187041
REFERENCE   2  (bases 1 to 12650)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2014) Department of Biology, The Pennsylvania
            State University, 208 Mueller Laboratory, University Park, PA 16802,
            USA
REFERENCE   3  (bases 1 to 12650)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12650)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.
            (2014) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-SEP-2014) Department of Biology, The Pennsylvania State
            University, 208 Mueller Laboratory, University Park, PA 16802, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    99..104
                     /label="non-unique SacI"
                     /note="non-unique SacI"
     terminator      complement(111..363)
                     /label="MAS terminator"
                     /note="mannopine synthase terminator"
     misc_feature    complement(373..406)
                     /label="BAR gene remnant"
                     /note="BAR gene remnant"
     misc_feature    407..412
                     /label="MCS2 KpnI site"
                     /note="MCS2 KpnI site"
     CDS             complement(416..931)
                     /note="RNA silencing suppressor p19 from Tomato bushy stunt
                     virus (strain Cherry). Accession#: P11690"
                     /label="RNA silencing suppressor p19"
     promoter        complement(935..1315)
                     /label="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2311..2656
                     /label="CaMV35S(short)"
                     /note="Cauliflower mosaic virus 35S promoter (short)"
     misc_feature    2659..2675
                     /label="XhoSac linker"
                     /note="XhoSac linker"
     misc_feature    2686..2740
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2742..3422
                     /codon_start=1
                     /gene="X-NmVen210"
                     /product="mcs1-NmVenus210 fusion protein"
                     /label="X-NmVen210"
                     /protein_id="AIN46605.1"
                     /translation="MGRALGTSGGSGGGSGMVSKGEELFTGVVPILVELDGDVNGHKFS
                     VSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSA
                     MPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYN
                     SHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQS
                     KLSK"
     gene            2742..3422
                     /gene="X-NmVen210"
                     /label="X-NmVen210"
     misc_feature    2742..2789
                     /label="MCS1 front end"
                     /note="MCS1 front end"
     misc_feature    2790..3422
                     /gene="X-NmVen210"
                     /label="NmVenus210 specific sequence"
                     /note="NmVenus210 specific sequence"
     CDS             2790..3308
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label="VN173"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     misc_difference 3408..3410
                     /gene="X-NmVen210"
                     /label="A206K monomerizing mutation"
                     /note="A206K monomerizing mutation"
     misc_feature    3423..3444
                     /label="MCS1 back end"
                     /note="MCS1 back end"
     terminator      3455..4162
                     /label="OCS terminator"
                     /note="octopine synthase terminator"
     misc_feature    4171..4176
                     /label="non-unique NdeI"
                     /note="non-unique NdeI"
     promoter        5163..5508
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5504..5528
                     /label="pDOE mcs3bF with Bsu36I"
                     /note="pDOE mcs3bF with Bsu36I"
     misc_feature    5511..5526
                     /label="Bsu361 linker"
                     /note="Bsu361 linker"
     regulatory      5527..5592
                     /label="TMV-Omega enhancer"
                     /note="TMV-Omega enhancer"
                     /regulatory_class="enhancer"
     misc_feature    5537..5591
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     misc_feature    5593..5640
                     /label="MCS3 front end"
                     /note="MCS3 front end"
     CDS             5641..5664
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
     CDS             5674..5697
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             5704..5727
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
     misc_feature    5740..5829
                     /gene="X-SFS-CVen210"
                     /label="CVenus210 specific sequence"
                     /note="CVenus210 specific sequence"
     misc_feature    5830..5848
                     /label="MCS3 back end"
                     /note="MCS3 back end"
     terminator      5849..6101
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6111..6127
                     /label="remnant from pFGC5941"
                     /note="remnant from pFGC5941"
     primer_bind     complement(6152..6168)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    6371..6395
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7696..8322
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             8754..9824
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      9893..10087
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    10431..10571
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10757..11345)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(11435..12226)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta cctcactctg
      421 actccttctc gaaggtttga gtaccttcag gacatcctct tgaaacatta gattcaactt
      481 caattggagc caactgcaaa agttcttgct tagacctaat tgccatctca cacaaatgtt
      541 gaagagttct tgatcctcca gaaacagtga tactaactcc cctgaaccta atagaataag
      601 tacatccgat ttgatcgaac ccaaagaatc ttgatgcagc atagttgact gaatctccag
      661 tccaagatcc aagcactcta tgcaatgatg cttcagttct atcgtatctg agatacctct
      721 taaacacaac ttttccgaaa ccccaagatt ccttgaaacc aagtggatta tcttgatttg
      781 agttcgtctc atcgttatgc aatctccact cagtccaaga tggactttca tcaggaagtt
      841 tgaaaggaga agttgtacct cctgatcctc catcccatct ttcagagtta gcttgttctc
      901 tagcatcatt tccttgtata gctcgttcca ttcgcgattt ggtgtatcga gattggttat
      961 gaaattcaga tgctagtgta atgtattggt aatttgggaa gatataatag gaagcaaggc
     1021 tatttatcca tttctgaaaa ggcgaaatgg cgtcaccgcg agcgtcacgc gcattccgtt
     1081 cttgctgtaa agcgttgttt ggtacacttt tgactagcga ggcttggcgt gtcagcgtat
     1141 ctattcaaaa gtcgttaatg gctgcggatc aagaaaaagt tggaatagaa acagaatacc
     1201 cgcgaaattc aggcccggtt gccatgtcct acacgccgaa ataaacgacc aaattagtag
     1261 aaaaataaaa actgactcgg atacttacgt cacgtcttgc gcactgattt gaaaaatctc
     1321 agaattccaa tcccacaaaa atctgagctt aacagcacag ttgctcctct cagagcagaa
     1381 tcgggtattc aacaccctca tatcaactac tacgttgtgt ataacggtcc acatgccggt
     1441 atatacgatg actggggttg tacaaaggcg gcaacaaacg gcgttcccgg agttgcacac
     1501 aagaaatttg ccactattac agaggcaaga gcagcagctg acgcgtacac aacaagtcag
     1561 caaacagaca ggttgaactt catccccaaa ggagaagctc aactcaagcc caagagcttt
     1621 gctaaggccc taacaagccc accaaagcaa aaagcccact ggctcacgct aggaaccaaa
     1681 aggcccagca gtgatccagc cccaaaagag atctcctttg ccccggagat tacaatggac
     1741 gatttcctct atctttacga tctaggaagg aagttcgaag gtgaaggtga cgacactatg
     1801 ttcaccactg ataatgagaa ggttagcctc ttcaatttca gaaagaatgc tgacccacag
     1861 atggttagag aggcctacgc agcaggtctc atcaagacga tctacccgag taacaatctc
     1921 caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt caggactaat
     1981 tgcatcaaga acacagagaa agacatattt ctcaagatca gaagtactat tccagtatgg
     2041 acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt ctctaaaaag
     2101 gtagttccta ctgaatctaa ggccatgcat ggagtctaag attcaaatcg aggatctaac
     2161 agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa
     2221 gaagaaaatc ttcgtcaaca tggtggagca cgacactctg gtctactcca aaaatgtcaa
     2281 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaagga taatttcggg
     2341 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtagaaaa
     2401 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca ttcaagatct
     2461 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     2521 agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca ctgacgtaag
     2581 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     2641 tcatttggag aggacacgct cgagtataag agctctattt ttacaacaat taccaacaac
     2701 aacaaacaac aaacaacatt acaattacat ttacaattac catggggcgc gccctaggaa
     2761 ctagtggagg atccggaggt gggtcaggaa tggtgagcaa gggcgaggag ctgttcaccg
     2821 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     2881 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca
     2941 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt
     3001 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     3061 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     3121 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     3181 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     3241 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
     3301 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     3361 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaag ctgagcaaat
     3421 aatctagacc cgggggttaa ttaaccctag agtcctgctt taatgagata tgcgagacgc
     3481 ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa acctgagcat
     3541 gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat cacccgttac
     3601 tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtaccc tactacttat
     3661 atgtacaata ttaaaatgaa aacaatatat tgtgctgaat aggtttatag cgacatctat
     3721 gatagagcgc cacaataaca aacaattgcg ttttattatt acaaatccaa ttttaaaaaa
     3781 agcggcagaa ccggtcaaac ctaaaagact gattacataa atcttattca aatttcaaaa
     3841 gtgccccagg ggctagtatc tacgacacac cgagcggcga actaataacg ctcactgaag
     3901 ggaactccgg ttccccgccg gcgcgcatgg gtgagattcc ttgaagttga gtattggccg
     3961 tccgctctac cgaaagttac gggcaccatt caacccggtc cagcacggcg gccgggtaac
     4021 cgacttgctg ccccgagaat tatgcagcat ttttttggtg tatgtgggcc ccaaatgaag
     4081 tgcaggtcaa accttgacag tgacgacaaa tcgttgggcg ggtccagggc gaattttgcg
     4141 acaacatgtc gaggctcagc aggacctgct catatgttcc aatcccacaa aaatctgagc
     4201 ttaacagcac agttgctcct ctcagagcag aatcgggtat tcaacaccct catatcaact
     4261 actacgttgt gtataacggt ccacatgccg gtatatacga tgactggggt tgtacaaagg
     4321 cggcaacaaa cggcgttccc ggagttgcac acaagaaatt tgccactatt acagaggcaa
     4381 gagcagcagc tgacgcgtac acaacaagtc agcaaacaga caggttgaac ttcatcccca
     4441 aaggagaagc tcaactcaag cccaagagct ttgctaaggc cctaacaagc ccaccaaagc
     4501 aaaaagccca ctggctcacg ctaggaacca aaaggcccag cagtgatcca gccccaaaag
     4561 agatctcctt tgccccggag attacaatgg acgatttcct ctatctttac gatctaggaa
     4621 ggaagttcga aggtgaaggt gacgacacta tgttcaccac tgataatgag aaggttagcc
     4681 tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac gcagcaggtc
     4741 tcatcaagac gatctacccg agtaacaatc tccaggagat caaatacctt cccaagaagg
     4801 ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa gaacacagag aaagacatat
     4861 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa
     4921 ggcaagtaat agagattgga gtctctaaaa aggtagttcc tactgaatct aaggccatgc
     4981 atggagtcta agattcaaat cgaggatcta acagaactcg ccgtgaagac tggcgaacag
     5041 ttcatacaga gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag
     5101 cacgacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga ccaaagggct
     5161 attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct
     5221 atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat
     5281 tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga
     5341 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
     5401 gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     5461 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg cctcaggtac
     5521 agagcatatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattaca
     5581 tttacaatta caatggggtc cctacgtagt cacgtgacgt ccggaggttc tggtggagct
     5641 tggtctcatc cacaattcga gaagtcagga ggagattata aggatgatga tgataaggct
     5701 tcttggtcac atcctcagtt tgaaaaggga tctgggtcag accccaacga gaagcgcgat
     5761 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     5821 tacaagtaag cgatcgccat ttaaatagga tcgttcaaac atttggcaat aaagtttctt
     5881 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     5941 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     6001 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     6061 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgttaacgat atgtcgaggc
     6121 tcagcaggac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact
     6181 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     6241 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     6301 gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac
     6361 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     6421 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
     6481 gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata
     6541 gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt
     6601 cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt
     6661 ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga
     6721 gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca
     6781 accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg
     6841 gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg
     6901 ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg
     6961 ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca
     7021 ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc
     7081 gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga
     7141 agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg
     7201 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc
     7261 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg
     7321 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc
     7381 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga
     7441 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc
     7501 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc
     7561 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga
     7621 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat
     7681 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
     7741 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
     7801 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
     7861 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
     7921 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
     7981 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
     8041 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
     8101 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
     8161 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
     8221 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
     8281 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
     8341 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
     8401 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
     8461 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
     8521 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg
     8581 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
     8641 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg
     8701 gttgcctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg
     8761 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     8821 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     8881 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     8941 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     9001 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     9061 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     9121 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     9181 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     9241 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     9301 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     9361 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     9421 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     9481 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     9541 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     9601 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     9661 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     9721 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     9781 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     9841 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata
     9901 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     9961 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
    10021 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
    10081 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
    10141 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
    10201 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
    10261 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
    10321 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
    10381 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
    10441 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
    10501 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
    10561 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    10621 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    10681 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    10741 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    10801 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    10861 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    10921 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10981 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    11041 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    11101 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    11161 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    11221 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    11281 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    11341 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    11401 gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat
    11461 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
    11521 gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt
    11581 ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga
    11641 tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct
    11701 ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc
    11761 aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta
    11821 agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg
    11881 catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga
    11941 cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga
    12001 cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat
    12061 cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc
    12121 ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt
    12181 tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc
    12241 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa
    12301 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
    12361 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca
    12421 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
    12481 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
    12541 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
    12601 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
//
