LOCUS       V007960                 8932 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007960
VERSION     V007960
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8932)
  AUTHORS   Ahmed A.
  TITLE     Double origin vectors for isolating bidirectional deletions for DNA
            sequence analysis
  JOURNAL   Gene In press
REFERENCE   2  (bases 1 to 8932)
  AUTHORS   Ahmed A.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-1993) Ahmed A., University of Alberta, Genetics,
            Biological Sciences Bldg Rm G502, Edmonton, Alberta, T6G 2E1, Canada
REFERENCE   3  (bases 1 to 8932)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8932)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene In
            press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-NOV-1993) Ahmed A., University of Alberta, Genetics, Biological
            Sciences Bldg Rm G502, Edmonton, Alberta, T6G 2E1, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1107..1898)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     RBS             2695..2703
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             2707..2904
                     /gene="cro"
                     /label="Regulatory protein cro"
                     /note="Regulatory protein cro from Escherichia phage
                     lambda. Accession#: P03040"
     terminator      complement(3195..3289)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     primer_bind     5861..5877
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(5974..5990)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5998..6014)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6022..6052)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6067..6088)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6376..6964)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7138..7995)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7996..8100)
                     /label="AmpR promoter"
     misc_feature    8749..8932
                     /label="cos"
                     /note="lambda cos site; allows packaging into phage lambda
                     particles"
ORIGIN
        1 aggtcgccgc cccgtaacct gtcggatcac cggaaaggac ccgtaaagtg ataatgatta
       61 tcatctacat atcacaacgt gcgtggaggc catcaaacca cgtcaaataa tcaattatga
      121 cgcaggtatc gtattaattg atctgcatca acttaacgta aaaacaactt cagacaatac
      181 aaatcagcga cactgaatac ggggcaacct catgtcaacg aagaacagaa cccgcagaac
      241 aacaacccgc aacatccgct ttcctaacca aatgattgaa caaattaaca tcgctcttga
      301 gcaaaaaggg tccgggaatt tctcagcctg ggtcattgaa gcctgccgtc ggagactaac
      361 gtcagaaaag agagcatata catcaattaa aagtgatgaa gaatgaacat cccgcgttct
      421 tccctccgaa caggacgata ttgtaaattc acttaattac gagggcattg cagtaattgg
      481 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
      541 tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
      601 agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag
      661 ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat
      721 ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt
      781 cacttaaaaa actcaggccg cagtcggtaa ccggcctctt catcgggaat gcgcgcgacc
      841 ttcagcatcg ccggcatgtc cccctggcgg acgggaagta tccagctcga tcaggcagca
      901 gctgaaccaa ctcgcgaggg gatcgagccc ggggtgggcg aagaactcca gcatgagatc
      961 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     1021 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
     1081 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     1141 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     1201 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     1261 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     1321 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
     1381 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     1441 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     1501 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     1561 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     1621 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     1681 gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac
     1741 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     1801 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     1861 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     1921 tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag
     1981 tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg
     2041 cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc
     2101 tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc
     2161 cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc
     2221 ttgcgccctg agtgcttgcg gcagcgtgaa gcttcaggtg agaacatccc tgcctgaaca
     2281 tgagaaaaaa cagggtactc atactcactt ctaagtgacg gctgcatact aaccgcttca
     2341 tacatctcgt agatttctct ggcgattgaa gggctaaatt cttcaacgct aactttgaga
     2401 atttttgcaa gcaatgcggc gttataagca tttaatgcat tgatgccatt aaataaagca
     2461 ccaacgcctg actgccccat ccccatcttg tctgcgacag attcctggga taagccaagt
     2521 tcatttttct ttttttcata aattgcttta aggcgacgtg cgtcctcaag ctgctcttgt
     2581 gttaatggtt tcttttttgt gctcatacgt taaatctatc accgcaaggg ataaatatct
     2641 aacaccgtgc gtgttgacta ttttacctct ggcggtgata atggttgcat gtactaagga
     2701 ggttgtatgg aacaacgcat aaccctgaaa gattatgcaa tgcgctttgg gcaaaccaag
     2761 acagctaaag atctcggcgt atatcaaagc gcgatcaaca aggccattca tgcaggccga
     2821 aagatttttt taactataaa cgctgatgga agcgtttatg cggaagaggt aaagcccttc
     2881 ccgagtaaca aaaaaacaac agcataaata accccgctct tacacattcc agccctgaaa
     2941 attgttatct aaggaaatac ttacatatgg ttcgtgcaaa caaacgcaac gaggctctac
     3001 gaatcgagag tgcgttgctt aacaaaatcg caatgcttgg aactgagaag acagcggaag
     3061 ctgtgggcgt tgataagtcg cagatcagca ggtggaagag ggactggatt ccaaagttct
     3121 caatgctgct tgctgttctt gaatgggggg tcgttgacga cgacatggct cgattggcgc
     3181 gacaagttgc tgcgattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     3241 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtca ttatgacaaa
     3301 tacagcaaaa atactcaact tcggcagagg taactttgcc ggacaggagc gtaatgtggc
     3361 agatctcgat gatggttacg ccagactatc aaatatgctg cttgaggctt attcgggcgc
     3421 agatctgacc aagcgacagt ttaaagtgct gcttgccatt ctgcgtaaaa cctatgggtg
     3481 gaataaacca atggacagaa tcaccgattc tcaacttagc gagattacaa agttacctgt
     3541 caaacggtgc aatgaagcca agttagaact cgtcagaatg aatattatca agcagcaagg
     3601 cggcatgttt ggaccaaata aaaacatctc agaatggtgc atccctcaaa acgagggaaa
     3661 atcccctaaa acgagggata aaacatccct caaattgggg gattgctatc cctcaaaaca
     3721 gggggacaca aaagacacta ttacaaaaga aaaaagaaaa gattattcgt cagagaattc
     3781 tggcgaatcc tctgaccagc cagaaaacga cctttctgtg gtgaaaccgg atgctgcaat
     3841 tcagagcggc agcaagtggg ggacagcaga agacctgacc gccgcagagt ggatgtttga
     3901 catggtgaag actatcgcac catcagccag aaaaccgaat tttgctgggt gggctaacga
     3961 tatccgcctg atgcgtgaac gtgacggacg taaccaccgc gacatgtgtg tgctgttccg
     4021 ctgggcatgc caggacaact tctggtccgg taacgtgctg agcccggcca aactccgcga
     4081 taagtggacc caactcgaaa tcaaccgtaa caagcaacag gcaggcgtga cagccagcaa
     4141 accaaaactc gacctgacaa acacagactg gatttacggg gtggatctat gaaaaacatc
     4201 gccgcacaga tggttaactt tgaccgtgag cagatgcgtc ggatcgccaa caacatgccg
     4261 gaacagtacg acgaaaagcc gcaggtacag caggtagcgc agatcatcaa cggtgtgttc
     4321 agccagttac tggcaacttt cccggcgagc ctggctaacc gtgaccagaa cgaagtgaac
     4381 gaaatccgtc gccagtgggt tctggctttt cgggaaaacg ggatcaccac gatggaacag
     4441 gttaacgcag gaatgcgcgt agcccgtcgg cagaatcgac catttctgcc atcacccggg
     4501 cagtttgttg catggtgccg ggaagaagca tccgttaccg ccggactgcc aaacgtcagc
     4561 gagctggttg atatggttta cgagtattgc cggaagcgag gcctgtatcc ggatgcggag
     4621 tcttatccgt ggaaatcaaa cgcgcactac tggctggtta ccaacctgta tcagaacatg
     4681 cgggccaatg cgcttactga tgcggaatta cgccgtaagg ccgcagatga gcttgtccat
     4741 atgactgcga gaattaaccg tggtgaggcg atccctgaac cagtaaaaca acttcctgtc
     4801 atgggcggta gacctctaaa tcgtgcacag gctctggcga agatcgcaga aatcaaagct
     4861 aagttcggac tgaaaggagc aagtgtatga cgggcaaaga ggcaattatt cattacctgg
     4921 ggacgcataa tagcttctgt gcgccggacg ttgccgcgct aacaggcgca acagtaacca
     4981 gcataaatca ggccgcggct aaaatggcac gggcaggtct tctggttatc gaaggtaagg
     5041 tctggcgaac ggtgtattac cggtttgcta ccagggaaga acgggaagga aagatgagca
     5101 cgaacctggt ttttaaggag tgtcgccaga gtgccgcgat gaaacgggta ttggcggtat
     5161 atggagttaa aagatgacca tctacattac tgagctaata acaggcctgc tggtaatcgc
     5221 aggccttttt atttggggga gagggaagtc atgaaaaaac taacctttga aattcgatct
     5281 ccagcacatc agcaaaacgc tattcacgca gtacagcaaa tccttccaga cccaaccaaa
     5341 ccaatcgtag taaccattca ggaacgcaac cgcagcttag accaaaacag gaagctatgg
     5401 gcctgcttag gtgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     5461 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     5521 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     5581 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     5641 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     5701 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     5761 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     5821 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     5881 ttcgagctcg gtacccgggg gcgcgccgga tccttaatta agtctagagt cgactgttta
     5941 aacctgcagg catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6001 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6061 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6121 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6181 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6241 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6301 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6541 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     6601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6781 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     6841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7141 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7201 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7261 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7321 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7381 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7441 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7501 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7561 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7621 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7681 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7741 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7801 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7861 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     7921 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     7981 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8041 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8101 cacatttccc cgaaaagtgc ggtaatgact ccaacttatt gatagtgttt tatgttcaga
     8161 taatgcccga tgactttgtc atgcagctcc accgattttg agaacgacag cgacttccgt
     8221 cccagccgtg ccaggtgctg cctcagattc aggttatgcc gctcaattcg ctgcgtatat
     8281 cgcttgctga ttacgtgcag ctttcccttc aggcgtacgc atgaccggcg gcggatttgg
     8341 cggctgtatc gtcgcgctga tcccggaaga gctggtgcct gccgtacagc aacgtgtcgc
     8401 tgaacaatat gaagcaaaaa caggtattaa agagactttt tacgtttgta aaccatcaca
     8461 aggagcagga cagtgctgaa cgaaactccc gcactggcac ccgatggtca gccgtaccga
     8521 ctgttgatct gcctcgctgg cctgccgcag ttcttcaacc tcccggcgca gcttttcgtt
     8581 ctcaatttca gcatcccttt cggcatacca ttttatgacg gcggcagagt cataaagcac
     8641 ctcattaccc ttgccaccgc ctcgcagaac gggcattccc tgttcctgcc agttctgaat
     8701 ggtacggata ctcgcaccga aaatgtcagc cagctgcttt ttgttgactt ccattgttca
     8761 ttccacggac aaaaacagag aaaggaaacg acagaggcca aaaagcctcg ctttcagcac
     8821 ctgtcgtttc ctttcttttc agagggtatt ttaaataaaa acattaagtt atgacgaaga
     8881 agaacggaaa cgccttaaac cggaaaattt tcataaatag cgaaaacccg cg
//
