LOCUS       V007962                11581 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007962
VERSION     V007962
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11581)
  AUTHORS   Ahmed A.
  TITLE     Double origin vectors for isolating bidirectional deletions for DNA
            sequence analysis
  JOURNAL   Gene In press
REFERENCE   2  (bases 1 to 11581)
  AUTHORS   Ahmed A.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-1993) Ahmed A., University of Alberta, Genetics,
            Biological Sciences Bldg Rm G502, Edmonton, Alberta, T6G 2E1, Canada
REFERENCE   3  (bases 1 to 11581)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11581)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene In
            press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-NOV-1993) Ahmed A., University of Alberta, Genetics, Biological
            Sciences Bldg Rm G502, Edmonton, Alberta, T6G 2E1, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1107..1898)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     RBS             2695..2703
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             2707..2904
                     /gene="cro"
                     /label="Regulatory protein cro"
                     /note="Regulatory protein cro from Escherichia phage
                     lambda. Accession#: P03040"
     terminator      complement(3195..3289)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             6575..7762
                     /label="TcR"
                     /note="tetracycline efflux protein"
     CDS             8406..8594
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    8699..8839
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9025..9613)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9787..10644)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10645..10749)
                     /label="AmpR promoter"
ORIGIN
        1 aggtcgccgc cccgtaacct gtcggatcac cggaaaggac ccgtaaagtg ataatgatta
       61 tcatctacat atcacaacgt gcgtggaggc catcaaacca cgtcaaataa tcaattatga
      121 cgcaggtatc gtattaattg atctgcatca acttaacgta aaaacaactt cagacaatac
      181 aaatcagcga cactgaatac ggggcaacct catgtcaacg aagaacagaa cccgcagaac
      241 aacaacccgc aacatccgct ttcctaacca aatgattgaa caaattaaca tcgctcttga
      301 gcaaaaaggg tccgggaatt tctcagcctg ggtcattgaa gcctgccgtc ggagactaac
      361 gtcagaaaag agagcatata catcaattaa aagtgatgaa gaatgaacat cccgcgttct
      421 tccctccgaa caggacgata ttgtaaattc acttaattac gagggcattg cagtaattgg
      481 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
      541 tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
      601 agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag
      661 ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat
      721 ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt
      781 cacttaaaaa actcaggccg cagtcggtaa ccggcctctt catcgggaat gcgcgcgacc
      841 ttcagcatcg ccggcatgtc cccctggcgg acgggaagta tccagctcga tcaggcagca
      901 gctgaaccaa ctcgcgaggg gatcgagccc ggggtgggcg aagaactcca gcatgagatc
      961 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     1021 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
     1081 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     1141 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     1201 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     1261 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     1321 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
     1381 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     1441 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     1501 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     1561 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     1621 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     1681 gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac
     1741 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     1801 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     1861 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     1921 tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag
     1981 tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg
     2041 cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc
     2101 tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc
     2161 cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc
     2221 ttgcgccctg agtgcttgcg gcagcgtgaa gcttcaggtg agaacatccc tgcctgaaca
     2281 tgagaaaaaa cagggtactc atactcactt ctaagtgacg gctgcatact aaccgcttca
     2341 tacatctcgt agatttctct ggcgattgaa gggctaaatt cttcaacgct aactttgaga
     2401 atttttgcaa gcaatgcggc gttataagca tttaatgcat tgatgccatt aaataaagca
     2461 ccaacgcctg actgccccat ccccatcttg tctgcgacag attcctggga taagccaagt
     2521 tcatttttct ttttttcata aattgcttta aggcgacgtg cgtcctcaag ctgctcttgt
     2581 gttaatggtt tcttttttgt gctcatacgt taaatctatc accgcaaggg ataaatatct
     2641 aacaccgtgc gtgttgacta ttttacctct ggcggtgata atggttgcat gtactaagga
     2701 ggttgtatgg aacaacgcat aaccctgaaa gattatgcaa tgcgctttgg gcaaaccaag
     2761 acagctaaag atctcggcgt atatcaaagc gcgatcaaca aggccattca tgcaggccga
     2821 aagatttttt taactataaa cgctgatgga agcgtttatg cggaagaggt aaagcccttc
     2881 ccgagtaaca aaaaaacaac agcataaata accccgctct tacacattcc agccctgaaa
     2941 attgttatct aaggaaatac ttacatatgg ttcgtgcaaa caaacgcaac gaggctctac
     3001 gaatcgagag tgcgttgctt aacaaaatcg caatgcttgg aactgagaag acagcggaag
     3061 ctgtgggcgt tgataagtcg cagatcagca ggtggaagag ggactggatt ccaaagttct
     3121 caatgctgct tgctgttctt gaatgggggg tcgttgacga cgacatggct cgattggcgc
     3181 gacaagttgc tgcgattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     3241 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtca ttatgacaaa
     3301 tacagcaaaa atactcaact tcggcagagg taactttgcc ggacaggagc gtaatgtggc
     3361 agatctcgat gatggttacg ccagactatc aaatatgctg cttgaggctt attcgggcgc
     3421 agatctgacc aagcgacagt ttaaagtgct gcttgccatt ctgcgtaaaa cctatgggtg
     3481 gaataaacca atggacagaa tcaccgattc tcaacttagc gagattacaa agttacctgt
     3541 caaacggtgc aatgaagcca agttagaact cgtcagaatg aatattatca agcagcaagg
     3601 cggcatgttt ggaccaaata aaaacatctc agaatggtgc atccctcaaa acgagggaaa
     3661 atcccctaaa acgagggata aaacatccct caaattgggg gattgctatc cctcaaaaca
     3721 gggggacaca aaagacacta ttacaaaaga aaaaagaaaa gattattcgt cagagaattc
     3781 tggcgaatcc tctgaccagc cagaaaacga cctttctgtg gtgaaaccgg atgctgcaat
     3841 tcagagcggc agcaagtggg ggacagcaga agacctgacc gccgcagagt ggatgtttga
     3901 catggtgaag actatcgcac catcagccag aaaaccgaat tttgctgggt gggctaacga
     3961 tatccgcctg atgcgtgaac gtgacggacg taaccaccgc gacatgtgtg tgctgttccg
     4021 ctgggcatgc caggacaact tctggtccgg taacgtgctg agcccggcca aactccgcga
     4081 taagtggacc caactcgaaa tcaaccgtaa caagcaacag gcaggcgtga cagccagcaa
     4141 accaaaactc gacctgacaa acacagactg gatttacggg gtggatctat gaaaaacatc
     4201 gccgcacaga tggttaactt tgaccgtgag cagatgcgtc ggatcgccaa caacatgccg
     4261 gaacagtacg acgaaaagcc gcaggtacag caggtagcgc agatcatcaa cggtgtgttc
     4321 agccagttac tggcaacttt cccggcgagc ctggctaacc gtgaccagaa cgaagtgaac
     4381 gaaatccgtc gccagtgggt tctggctttt cgggaaaacg ggatcaccac gatggaacag
     4441 gttaacgcag gaatgcgcgt agcccgtcgg cagaatcgac catttctgcc atcacccggg
     4501 cagtttgttg catggtgccg ggaagaagca tccgttaccg ccggactgcc aaacgtcagc
     4561 gagctggttg atatggttta cgagtattgc cggaagcgag gcctgtatcc ggatgcggag
     4621 tcttatccgt ggaaatcaaa cgcgcactac tggctggtta ccaacctgta tcagaacatg
     4681 cgggccaatg cgcttactga tgcggaatta cgccgtaagg ccgcagatga gcttgtccat
     4741 atgactgcga gaattaaccg tggtgaggcg atccctgaac cagtaaaaca acttcctgtc
     4801 atgggcggta gacctctaaa tcgtgcacag gctctggcga agatcgcaga aatcaaagct
     4861 aagttcggac tgaaaggagc aagtgtatga cgggcaaaga ggcaattatt cattacctgg
     4921 ggacgcataa tagcttctgt gcgccggacg ttgccgcgct aacaggcgca acagtaacca
     4981 gcataaatca ggccgcggct aaaatggcac gggcaggtct tctggttatc gaaggtaagg
     5041 tctggcgaac ggtgtattac cggtttgcta ccagggaaga acgggaagga aagatgagca
     5101 cgaacctggt ttttaaggag tgtcgccaga gtgccgcgat gaaacgggta ttggcggtat
     5161 atggagttaa aagatgacca tctacattac tgagctaata acaggcctgc tggtaatcgc
     5221 aggccttttt atttggggga gagggaagtc atgaaaaaac taacctttga aattcgatct
     5281 ccagcacatc agcaaaacgc tattcacgca gtacagcaaa tccttccaga cccaaccaaa
     5341 ccaatcgtag taaccattca ggaacgcaac cgcagcttag accaaaacag gaagctatgg
     5401 gcctgcttag gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg
     5461 gctaaatcgc gccagcgctg gctgttttac gcgttttgcc acgatcgggt ccagttcatg
     5521 cgcaacagcg ttgaactctt caatggtgac atcacgcagg gctggctgct ggaagaaacg
     5581 cgcaccggtt tcgcactgtt cacgacgggt gttgtattcg ctgccaacca gggtacgttt
     5641 gaagttactg ttgatgatga cgacagccac acctttgggc atggaaactg ctttggtccc
     5701 cagtgagcgg caatcgatca gcaaggcatg atctttcttg ccgagcgcgg aaattagctg
     5761 atccatgatc ccgcagttac agcctacaaa ctggttttct gcttcctgac cgttaagcgc
     5821 gatttgtgcg ccgtccagcg gcagatgata aagctgctgc aatacggttc cgaccgcgac
     5881 ttccagtgaa gcggaagaac ttaacccggc accctgcggc acattgccgt gatcaaccat
     5941 gtccacgccg ccgaagctgt tgttacgcag ttgcagatgt ttcaccacgc cacgaacgta
     6001 gttagcccat tgatagtttt catgtgcgac aatgggcgca tcgagggaaa actcgtcgag
     6061 ctgattttca taatcggctg ccatcacgcg aactttacgg tcatcgcgtg gtgcacaact
     6121 gatcacggtt tgataatcaa tcgcgcaggg cagaacgaaa ccgtcgttgt agtcggtgtg
     6181 ttcaccaatc aaattcacgc ggccaggcgc ctgaatggtg tgagtggcag ggtagccaaa
     6241 tgcgttggca aacagagatt gtgttttttc tttcagactc atttcttaca ctccggattc
     6301 gcgacggcct acagcaacct gggcgatgta tggcatcagg ttattcggaa tgccttgcgg
     6361 atcttcgccc atatcgcccg acggatgcgc gccaaccggg ttgaagtagc gcacgagggc
     6421 aatgctccag tccggctggg ctttttgcag atcggtgagg atctgttcca ccatcagctt
     6481 gcttttgccg taagggcttt gcggtgtgcc ggtcgggaag ctataatgcg gtagtttatc
     6541 acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt
     6601 catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc
     6661 gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct
     6721 agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg
     6781 ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat
     6841 catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac
     6901 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
     6961 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     7021 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
     7081 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
     7141 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
     7201 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
     7261 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg
     7321 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc
     7381 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct
     7441 gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct
     7501 tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga
     7561 tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat
     7621 cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt
     7681 ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc
     7741 atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc
     7801 actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct
     7861 tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc
     7921 agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta
     7981 ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga
     8041 agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc
     8101 cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc
     8161 tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc
     8221 tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta
     8281 ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc
     8341 ctctctcgtt tcatcggtat cattaccccc atgaacagaa attccccctt acacggaggc
     8401 atcaagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga
     8461 cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg
     8521 aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg
     8581 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     8641 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     8701 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     8761 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     8821 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8881 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8941 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     9001 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9061 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9121 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9181 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     9241 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9301 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9361 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9421 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     9481 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9541 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9601 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9661 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9721 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9781 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9841 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9901 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9961 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    10021 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    10081 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    10141 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    10201 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    10261 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10321 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10381 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
    10441 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10501 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10561 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10621 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10681 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10741 ggttccgcgc acatttcccc gaaaagtgcg gtaatgactc caacttattg atagtgtttt
    10801 atgttcagat aatgcccgat gactttgtca tgcagctcca ccgattttga gaacgacagc
    10861 gacttccgtc ccagccgtgc caggtgctgc ctcagattca ggttatgccg ctcaattcgc
    10921 tgcgtatatc gcttgctgat tacgtgcagc tttcccttca ggcgtacgca tgaccggcgg
    10981 cggatttggc ggctgtatcg tcgcgctgat cccggaagag ctggtgcctg ccgtacagca
    11041 acgtgtcgct gaacaatatg aagcaaaaac aggtattaaa gagacttttt acgtttgtaa
    11101 accatcacaa ggagcaggac agtgctgaac gaaactcccg cactggcacc cgatggtcag
    11161 ccgtaccgac tgttgatctg cctcgctggc ctgccgcagt tcttcaacct cccggcgcag
    11221 cttttcgttc tcaatttcag catccctttc ggcataccat tttatgacgg cggcagagtc
    11281 ataaagcacc tcattaccct tgccaccgcc tcgcagaacg ggcattccct gttcctgcca
    11341 gttctgaatg gtacggatac tcgcaccgaa aatgtcagcc agctgctttt tgttgacttc
    11401 cattgttcat tccacggaca aaaacagaga aaggaaacga cagaggccaa aaagcctcgc
    11461 tttcagcacc tgtcgtttcc tttcttttca gagggtattt taaataaaaa cattaagtta
    11521 tgacgaagaa gaacggaaac gccttaaacc ggaaaatttt cataaatagc gaaaacccgc
    11581 g
//
