LOCUS       40924_14890        9820 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDO184, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Ahmed A.
  TITLE     Double origin vectors for isolating bidirectional deletions for DNA 
            sequence analysis
  JOURNAL   Gene In press
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   Ahmed A.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-1993) Ahmed A., University of Alberta, Genetics, 
            Biological Sciences Bldg Rm G502, Edmonton, Alberta, T6G 2E1, Canada
REFERENCE   3  (bases 1 to 9820)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9820)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene In 
            press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-NOV-1993) Ahmed A., University of Alberta, Genetics, Biological 
            Sciences Bldg Rm G502, Edmonton, Alberta, T6G 2E1, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2549..3340
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      3490..4034
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     protein_bind    4274..4295
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4310..4340
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4348..4364
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4372..4388
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4398..4454
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(4458..4474)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6311..6499
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    6604..6744
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6930..7518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7692..8549)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8550..8654)
                     /label=AmpR promoter
     misc_feature    9637..9820
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
ORIGIN
        1 aggtcgccgc cccgtaacct gtcggatcac cggaaaggac ccgtaaagtg ataatgatta
       61 tcatctacat atcacaacgt gcgtggaggc catcaaacca cgtcaaataa tcaattatga
      121 cgcaggtatc gtattaattg atctgcatca acttaacgta aaaacaactt cagacaatac
      181 aaatcagcga cactgaatac ggggcaacct catgtcaacg aagaacagaa cccgcagaac
      241 aacaacccgc aacatccgct ttcctaacca aatgattgaa caaattaaca tcgctcttga
      301 gcaaaaaggg tccgggaatt tctcagcctg ggtcattgaa gcctgccgtc ggagactaac
      361 gtcagaaaag agagcatata catcaattaa aagtgatgaa gaatgaacat cccgcgttct
      421 tccctccgaa caggacgata ttgtaaattc acttaattac gagggcattg cagtaattgg
      481 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
      541 tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
      601 agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag
      661 ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat
      721 ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt
      781 cacttaaaaa actcaggccg cagtcggtaa ccggcctctt catcgggaat gcgcgcgacc
      841 ttcagcatcg ccggcatgtc cccctggcgg acgggaagta tccagctcga catcgtctgc
      901 gcggaaatgg acgaacagtg gggctatgtc ggggctaaat cgcgccagcg ctggctgttt
      961 tacgcgtttt gccacgatcg ggtccagttc atgcgcaaca gcgttgaact cttcaatggt
     1021 gacatcacgc agggctggct gctggaagaa acgcgcaccg gtttcgcact gttcacgacg
     1081 ggtgttgtat tcgctgccaa ccagggtacg tttgaagtta ctgttgatga tgacgacagc
     1141 cacacctttg ggcatggaaa ctgctttggt ccccagtgag cggcaatcga tcagcaaggc
     1201 atgatctttc ttgccgagcg cggaaattag ctgatccatg atcccgcagt tacagcctac
     1261 aaactggttt tctgcttcct gaccgttaag cgcgatttgt gcgccgtcca gcggcagatg
     1321 ataaagctgc tgcaatacgg ttccgaccgc gacttccagt gaagcggaag aacttaaccc
     1381 ggcaccctgc ggcacattgc cgtgatcaac catgtccacg ccgccgaagc tgttgttacg
     1441 cagttgcaga tgtttcacca cgccacgaac gtagttagcc cattgatagt tttcatgtgc
     1501 gacaatgggc gcatcgaggg aaaactcgtc gagctgattt tcataatcgg ctgccatcac
     1561 gcgaacttta cggtcatcgc gtggtgcaca actgatcacg gtttgataat caatcgcgca
     1621 gggcagaacg aaaccgtcgt tgtagtcggt gtgttcacca atcaaattca cgcggccagg
     1681 cgcctgaatg gtgtgagtgg cagggtagcc aaatgcgttg gcaaacagag attgtgtttt
     1741 ttctttcaga ctcatttctt acactccgga ttcgcgacgg cctacagcaa cctgggcgat
     1801 gtatggcatc aggttattcg gaatgccttg cggatcttcg cccatatcgc ccgacggatg
     1861 cgcgccaacc gggttgaagt agcgcacgag ggcaatgctc cagtccggct gggctttttg
     1921 cagatcggtg aggatctgtt ccaccatcag cttgcttttg ccgtaagggc tttgcggtgt
     1981 gccggtcggg aagctataat gcggtagttt atcacagtta aattgctaac gcagtcaggc
     2041 accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc
     2101 tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata tcgtccattc
     2161 cgacagcatc gccagtcact atggcgtgct gctagcttca cgctgccgca agcactcagg
     2221 gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg
     2281 accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag
     2341 aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac
     2401 agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa
     2461 agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga
     2521 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     2581 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     2641 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     2701 cgacctgtcc ggtgccctga atgaactcca agacgaggca gcgcggctat cgtggctggc
     2761 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     2821 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     2881 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     2941 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     3001 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     3061 cgccaggctc aaggcgcgga tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     3121 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     3181 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     3241 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     3301 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     3361 gcgatgataa gctgtcaaac atgagaatta caacttatat cgtatggggc tgacttcagg
     3421 tgctacattt gaagagataa attgcactga aatctagaaa tattttatct gattaataag
     3481 atgatcttct tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac
     3541 cgccttgcag ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa
     3601 ctggcttgga ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca
     3661 tgacttcaag actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg
     3721 catgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact
     3781 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg
     3841 tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag
     3901 gcaggaacag gagagcgcac gagggagccg ccaggggaaa cgcctggtat ctttatagtc
     3961 ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc
     4021 ggagcctatg gaaaaacggc tttgccttct ttcctgcgtt atcccctgat tctgtggata
     4081 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4141 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4201 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4261 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     4321 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     4381 gctatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc
     4441 aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4501 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4561 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcgat
     4621 aagctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc
     4681 gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac
     4741 gcgatcatgg cgaccacacc cgtcctgtgg atcctctacg ccggacgcat cgtggccggc
     4801 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     4861 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     4921 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     4981 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     5041 gagcgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     5101 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     5161 gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg
     5221 atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact
     5281 ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac
     5341 gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg
     5401 attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag
     5461 gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact
     5521 tcgatcattg gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac
     5581 gggttggcat ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc
     5641 ggtgcatgga gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat
     5701 tcaccactcc aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca
     5761 acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct
     5821 cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc
     5881 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa
     5941 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg
     6001 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc
     6061 ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga
     6121 agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt
     6181 gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga
     6241 gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaattcc cccttacacg
     6301 gaggcatcaa gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag
     6361 ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat
     6421 ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg
     6481 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     6541 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     6601 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     6661 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     6721 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6781 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6841 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6901 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6961 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7021 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7081 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     7141 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7201 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7261 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7321 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     7381 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7441 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7501 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7561 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7621 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7681 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7741 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7801 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7861 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7921 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7981 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg
     8041 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8101 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8161 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8221 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8281 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
     8341 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8401 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8461 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8521 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8581 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8641 ataggggttc cgcgcacatt tccccgaaaa gtgcggtaat gactccaact tattgatagt
     8701 gttttatgtt cagataatgc ccgatgactt tgtcatgcag ctccaccgat tttgagaacg
     8761 acagcgactt ccgtcccagc cgtgccaggt gctgcctcag attcaggtta tgccgctcaa
     8821 ttcgctgcgt atatcgcttg ctgattacgt gcagctttcc cttcaggcgg gattcataca
     8881 gcggccagcc atccgtcatc catatcacca cgtcaaaggg tgacagcagg ctcataagac
     8941 gccccagcgt cgccatagtg cgttcaccga atacgtgcgc aacaaccgtc ttccggagac
     9001 tgtcatacgc gtgcgtcata tactgactga aaacgcccgc accgttgaag ctgccagcgc
     9061 gctggagcaa ggcgacctga aacgtatggg cgagttgatg gcggagtctc atgcctctat
     9121 gcgcgatgat ttcgaaatca ccgtgccgca aattgacact ctggtagaaa tcgtcaaagc
     9181 tgtgattggc gacaaaggtg gcgtacgcat gaccggcggc ggatttggcg gctgtatcgt
     9241 cgcgctgatc ccggaagagc tggtgcctgc cgtacagcaa cgtgtcgctg aacaatatga
     9301 agcaaaaaca ggtattaaag agacttttta cgtttgtaaa ccatcacaag gagcaggaca
     9361 gtgctgaacg aaactcccgc actggcaccc gatggtcagc cgtaccgact gttgatctgc
     9421 ctcgctggcc tgccgcagtt cttcaacctc ccggcgcagc ttttcgttct caatttcagc
     9481 atccctttcg gcataccatt ttatgacggc ggcagagtca taaagcacct cattaccctt
     9541 gccaccgcct cgcagaacgg gcattccctg ttcctgccag ttctgaatgg tacggatact
     9601 cgcaccgaaa atgtcagcca gctgcttttt gttgacttcc attgttcatt ccacggacaa
     9661 aaacagagaa aggaaacgac agaggccaaa aagcctcgct ttcagcacct gtcgtttcct
     9721 ttcttttcag agggtatttt aaataaaaac attaagttat gacgaagaag aacggaaacg
     9781 ccttaaaccg gaaaattttc ataaatagcg aaaacccgcg
//
