LOCUS       40924_14880        4161 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDNR-LIB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Kalnine N, Farmer A, Hsieh F, Zhang R.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2006) Informatics, Clontech, 1290 Terra Bella 
            ave., Mountain View, CA 94043, USA
REFERENCE   2  (bases 1 to 4161)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4161)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (02-JUN-2006) Informatics, Clontech, 1290 Terra Bella ave., Mountain
            View, CA 94043, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    9..42
                     /label=loxP recombination site
                     /note="loxP recombination site"
     protein_bind    9..42
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    45..99
                     /note="MCS A; multiple cloning site"
     polyA_signal    162..296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    292..351
                     /note="MCS B; multiple cloning site"
     terminator      complement(415..509)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(533..1189)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     protein_bind    complement(1218..1251)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1305..2723)
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     promoter        complement(2724..3169)
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     rep_origin      3360..3948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4065..4081
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4089..4107
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgcat aacttcgtat agcatacatt atacgaagtt atcagtcgac ggtaccggac
       61 atatgcccgg gaattcggcc attacggcct gcaggatccg aaaaaacctc ccacacctcc
      121 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
      181 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      241 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccggc
      301 cgcctcggcc ctcgagaagc tttctagacc attcgtttgg cgcgcgggcc cagtaggtaa
      361 gtgaacatgg tcatagctgt ttccctagga gatcctggta tgactagtgc ttggattctc
      421 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
      481 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
      541 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
      601 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
      661 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
      721 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
      781 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
      841 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
      901 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
      961 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     1021 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     1081 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     1141 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     1201 tagctcctga aagatccata acttcgtata gcatacatta tacgaagtta tgcggccgcg
     1261 acgtcaatgc caataggata tcggcatttt cttttgcgtt tttatttgtt aactgttaat
     1321 tgtccttgtt caaggatgct gtctttgaca acagatgttt tcttgccttt gatgttcagc
     1381 aggaagctag gcgcaaacgt tgattgtttg tctgcgtaga atcctctgtt tgtcatatag
     1441 cttgtaatca cgacattgtt tcctttcgct tgaggtacag cgaagtgtga gtaagtaaag
     1501 gttacatcgt taggatcaag atccattttt aacacaaggc cagttttgtt cagcggcttg
     1561 tatgggccag ttaaagaatt agaaacataa ccaagcatgt aaatatcgtt agacgtaatg
     1621 ccgtcaatcg tcatttttga tccgcgggag tcagtgaaca ggtaccattt gccgttcatt
     1681 ttaaagacgt tcgcgcgttc aatttcatct gttactgtgt tagatgcaat cagcggtttc
     1741 atcacttttt tcagtgtgta atcatcgttt agctcaatca taccgagagc gccgtttgct
     1801 aactcagccg tgcgtttttt atcgctttgc agaagttttt gactttcttg acggaagaat
     1861 gatgtgcttt tgccatagta tgctttgtta aataaagatt cttcgccttg gtagccatct
     1921 tcagttccag tgtttgcttc aaatactaag tatttgtggc ctttatcttc tacgtagtga
     1981 ggatctctca gcgtatggtt gtcgcctgag ctgtagttgc cttcatcgat gaactgctgt
     2041 acattttgat acgtttttcc gtcaccgtca aagattgatt tataatcctc tacaccgttg
     2101 atgttcaaag agctgtctga tgctgatacg ttaacttgtg cagttgtcag tgtttgtttg
     2161 ccgtaatgtt taccggagaa atcagtgtag aataaacgga tttttccgtc agatgtaaat
     2221 gtggctgaac ctgaccattc ttgtgtttgg tcttttagga tagaatcatt tgcatcgaat
     2281 ttgtcgctgt ctttaaagac gcggccagcg tttttccagc tgtcaataga agtttcgccg
     2341 actttttgat agaacatgta aatcgatgtg tcatccgcat ttttaggatc tccggctaat
     2401 gcaaagacga tgtggtagcc gtgatagttt gcgacagtgc cgtcagcgtt ttgtaatggc
     2461 cagctgtccc aaacgtccag gccttttgca gaagagatat ttttaattgt ggacgaatcg
     2521 aactcaggaa cttgatattt ttcatttttt tgctgttcag ggatttgcag catatcatgg
     2581 cgtgtaatat gggaaatgcc gtatgtttcc ttatatggct tttggttcgt ttctttcgca
     2641 aacgcttgag ttgcgcctcc tgccagcagt gcggtagtaa aggttaatac tgttgcttgt
     2701 tttgcaaact ttttgatgtt catcgttcat gtctcctttt ttatgtactg tgttagcggt
     2761 ctgcttcttc cagccctcct gtttgaagat ggcaagttag ttacgcacaa taaaaaaaga
     2821 cctaaaatat gtaaggggtg acgccaaagt atacactttg ccctttacac attttaggtc
     2881 ttgcctgctt tatcagtaac aaacccgcgc gatttacttt tcgacctcat tctattagac
     2941 tctcgtttgg attgcaactg gtctattttc ctcttttgtt tgatagaaaa tcataaaagg
     3001 atttgcagac tacgggccta aagaactaaa aaatctatct gtttcttttc attctctgta
     3061 ttttttatag tttctgttgc atgggcataa agttgccttt ttaatcacaa ttcagaaaat
     3121 atcataatat ctcatttcac taaataatag tgaacggcag gtatatgtgg cgcgcctaag
     3181 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     3241 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3301 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3361 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3421 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3481 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3541 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3601 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3661 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3721 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3781 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3841 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3901 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3961 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4021 ttatcccctg attctgtgga taaccgtatt accgccttac gcgtgtaaaa cgacggccag
     4081 tagatctgta atacgactca ctatagggcg ctagctcgcc gcagccgaac gaccgagcgc
     4141 agcgagtcag tgagcgagga a
//
