LOCUS       V007970                 8059 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V007970
VERSION     V007970
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Veltman DM, Keizer-Gunnink I, Haastert PJ.
  TITLE     An extrachromosomal, inducible expression system for Dictyostelium
            discoideum
  JOURNAL   Plasmid 61 (2), 119-125 (2009)
   PUBMED   19046986
REFERENCE   2  (bases 1 to 8059)
  AUTHORS   Veltman DM, Keizer I, van Haastert PJM.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2008) Cell Biochemistry, Rijks Universiteit
            Groningen, Kerklaan 30, Haren, Groningen 9751 NN, The Netherlands
REFERENCE   3  (bases 1 to 8059)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8059)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2009"; volume: "61"; issue: "2"; pages: "119-125"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-JUL-2008) Cell Biochemistry, Rijks Universiteit Groningen,
            Kerklaan 30, Haren, Groningen 9751 NN, The Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..298
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     regulatory      473..734
                     /label="act8 terminator"
                     /note="act8 terminator"
                     /regulatory_class="terminator"
     regulatory      complement(741..995)
                     /label="mhcA terminator"
                     /note="mhcA terminator"
                     /regulatory_class="terminator"
     CDS             complement(1005..1424)
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     regulatory      complement(1436..1657)
                     /label="act6 promoter"
                     /note="act6 promoter"
                     /regulatory_class="promoter"
     promoter        1712..1816
                     /label="AmpR promoter"
     CDS             1817..2674
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2848..3436
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     regulatory      3649..3932
                     /label="act15 promoter"
                     /note="act15 promoter"
                     /regulatory_class="promoter"
     CDS             3945..4565
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     CDS             4587..4820
                     /label="VP16 AD"
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et
                     al., 1989)"
     regulatory      4862..5123
                     /label="act8 terminator"
                     /note="act8 terminator"
                     /regulatory_class="terminator"
     rep_origin      complement(5248..5790)
                     /direction=LEFT
                     /label="Ddp1 ori"
                     /note="Ddp1 ori"
     CDS             5700..6293
                     /codon_start=1
                     /product="G5"
                     /label="G5"
                     /protein_id="ACG80845.1"
                     /translation="MFNHIKDSGYYATNEEIEIFLESCTLCKEITAQTKRNSYKKRNII
                     NKLPEEEEEEEEEEEEEEEEEEQEEEVEKPTISEEEEEETPAVSEEEKEEEEQEEDKEK
                     DKEKKIEEDTETGKKKDEVMNERQDGIEISEKTNDKPTEKTKVKKNVSRVKDINLHKKG
                     NPITPKKIKNFSQQQLRLASGVSNEKRAIITLKK"
     CDS             complement(6301..7065)
                     /codon_start=1
                     /product="D5"
                     /label="D5"
                     /protein_id="ACG80846.1"
                     /translation="MYSTTLLFFLFFFIVSKSSVLQNDQIEVKYFQLLIVMYQKENCVE
                     KSMTGAVIYDECNIHGRVETNSTHALFYDDIETNNSRCNNFRNLTNLIKLNECINDEFG
                     ESILYKEYNETDDGYLFRVEDSFVEITSLSMDCTKNSKTIIEKFNICSKFENVYHITNI
                     TQEKSNRFTCTDPLCHYCKNENIQNNLDFKTTKCTPKYGASDSEFLSTIYNPKLDGSNN
                     GMEKSVTQEKNISNNLKINIYLIFFLIIFLIK"
     CDS             complement(7568..8059)
                     /codon_start=1
                     /product="D1'"
                     /label="D1'"
                     /protein_id="ACG80847.1"
                     /translation="MDFEKVNSNYYKIQSESTQINSNEIADLKKFIKEEVNKTSSKIDF
                     FLVSSTDALSNPENYSLLEVKCINCHSLCQGKNLYISCTRDGCQNNICYNCLGININIY
                     NVVINSKLCPPCFNDSVINKKCAMCSKNGTKCNLNQECKLHLCAQCSKKCLYILRVKTN
                     "
ORIGIN
        1 aagcttccgg gggatcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
       61 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
      121 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
      181 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
      241 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
      301 agtgaaagtc gagctcggta cccaattttt tttttttttt tttttttttt tttttttttt
      361 ttttcagatt gcataaaaag attttttttt tttttttttc ttatttctta aaacaaataa
      421 attaaattaa ataaaaaata aaaatcagat ctagtactag ttaaataaat aaattattta
      481 ataaataata aaaaaacaaa ttgttgtaat aatctaatat tttctttttt ttttaatttt
      541 ttttttttaa atcttaataa ttattaagtt attttaattt tttttttttt tttttttttt
      601 tttttttttt tttttctatc aaaaaaatca aatatattta aaaaatttat tatttacaga
      661 tacattttga atggtgaaga taaatatatg cattagatgt aaaacagcca aagagtatga
      721 aaatcaaaaa gatactcgag attttattta atatactaaa taataaaaaa gttaaaaaat
      781 gatcattgga taaatttttt ataattataa ataaagataa taattttttt tttaacaaaa
      841 ctaaaaataa aaataataaa ataattgtta aaataggttt tttttttttt tttttttttt
      901 taataaatgg tatttattaa tttatttgtt gtgtgtgttt ttttttttat aatatttttt
      961 tttttagcat tgaattaaga agaaatcaaa ttgattctag tttaatttcg ggtatatttg
     1021 agtggaatga gttcttcaat cgtagttttg actaacttgc cattcatttc tattaacaca
     1081 aaacaatctg gtgcatagtc tgaaatcaac tccctacaca taccacaagg acttaccact
     1141 cgaatacttc tatctacttc gtcagaataa gggtgtctaa cagctacaat cgtgtcaaaa
     1201 tccttttgtc cattcgaaac tgcactacca atcgcaatgg cttctgcaca aacagttact
     1261 cgtcctatat acgcttcaat atgtactgcc gaaatgattt ctcctgtttt cgtacgaatt
     1321 gccgctccca catgatgttt attatcctca taaagcattg taatcttctc tgtcgctact
     1381 tctactaatt ctagatcctg ttgagaaatg ttaaattgat ccattttgga tcttttatat
     1441 tatatttatt tattgattat ttttttgaat taattaaaaa aaaaaaaaat ttcattttat
     1501 aatctcagaa acctcaaaaa aaaaaaaata aaaaataaaa aatataaaaa aataaaaata
     1561 aaatcccaat tttaaagcga aaaaccaccc atggtttgaa aatttcaatc aatttcaaat
     1621 aactttactt aaaaaaaacc cattttttat ttaaaaaatc tagtggatcc cgctacaggg
     1681 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1741 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1801 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1861 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1921 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1981 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     2041 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     2101 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     2161 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     2221 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     2281 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     2341 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     2401 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     2461 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     2521 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2581 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2641 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2701 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2761 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2821 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2881 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2941 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     3001 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3061 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3121 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3181 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3241 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3301 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3361 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3421 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3481 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3541 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3601 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctgccggcta aaaaaaattt
     3661 ttatttattt ttatttattt tgaattaaat agattacaaa ttaattaatc ccatcaaatc
     3721 tttaaaaaaa aatggtttaa aaaaacttgg gttggttaat tattatttga aaattttaaa
     3781 acccaaatta aaaaaaaaaa atgggattca aaaatttttt tttttttttt tttttttttt
     3841 tttttttttt tttcagattg cataaaaaga tttttttttt ttttttttct tatttcttaa
     3901 aacaaataaa ttaaattaaa taaaaaataa aaatcagatc caaaatgtca agattagata
     3961 aaagtaaagt gattaacggc gcattagagc tgcttaatgg ggtcggaatc gaaggtttaa
     4021 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
     4081 aaaataagcg ggctttgctc gacgccttac ccattgagat gttagatagg caccatactc
     4141 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
     4201 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
     4261 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
     4321 cactagagaa tgcattatat gcactcagcg ctgtggggca ctttacttta ggttgcgtat
     4381 tggaagaaca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
     4441 tgccgccatt attacgacaa gctatcgaat tatttgatcg ccaaggtgca gagccagcct
     4501 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
     4561 gatcacacac tcgtagactc tcaactgccc caccaaccga tgtcagcctg ggggacgagc
     4621 tccacttaga cggcgaggac gtggcgatgg cgcatgccga cgcgctagac gatttcgatc
     4681 tggacatgtt gggggacggg gattccccgg gcccgggatt taccccccac gactccgccc
     4741 cctacggcgc tctggatatg gccgacttcg agtttgagca gatgtttacc gatgcccttg
     4801 gaattgacga gtacggtggt taatctagta cgcgtaaatt gttcacagaa aatgtttcta
     4861 aattatttaa taaataataa aaaaacaaat tgttgtaata atctaatatt ttcttttttt
     4921 tttaattttt tttttttaaa tcttaataat tattaagtta ttttaatttt tttttttttt
     4981 tttttttttt tttttttttt ttttctatca aaaaaatcaa atatatttaa aaaatttatt
     5041 atttacagat acattttgaa tggtgaagat aaatatatgc attagatgta aaacagccaa
     5101 agagtatgaa aatcaaaaag atagccggcc tcgacatttg aaattacaat gaatgttttt
     5161 cggtttttat tttttacttt ttatttgatt tttttttttt tttttttttt tttttttttt
     5221 ttaaaatttt tttttttttt ttttcaaatt tttttttttt taaaaaaaaa aaaattcatt
     5281 tttgtattta aattatttta taaataaaaa atgatagtag tagaaattgt tttttggaca
     5341 agaatcgtaa agtcaaaata ttcagttgat gatgaaatct ttaaatattt ggttaataga
     5401 gcaaaagttg tgtataacaa ttcagattat aaaacactat ctcaaactga tttagagaaa
     5461 tgggcgattc aaaacactca cagaacaata acttcaatta aaaatcgttt ttcagtcaaa
     5521 aagattggag atgaagaaaa gttatttaga atttcaaaaa atggtaaaat ccatctttat
     5581 acatgttcat tttaatttat tttaactaat tttttaaatt aggggagctt attgttctta
     5641 atgaattaga attcgataat tttcatatta aagaaggaaa gcatttaaga aaatcaaaaa
     5701 tgtttaatca tatcaaagac agtggttatt atgcaacaaa cgaagaaatt gaaatttttc
     5761 ttgaaagctg cactctatgt aaggaaatta ctgcacaaac caaacgtaat tcatataaaa
     5821 aaagaaatat aattaataaa ttaccggaag aagaagaaga agaagaagaa gaagaagaag
     5881 aagaagaaga agaagaagaa caagaagaag aagtagaaaa accgacaata tcggaagaag
     5941 aagaagaaga aacacctgca gtatcagaag aagaaaaaga agaggaagaa caagaagaag
     6001 ataaagaaaa agataaagaa aaaaaaatag aagaagatac agaaacagga aaaaaaaaag
     6061 atgaagttat gaatgaacgt caagatggta ttgaaattag tgaaaaaaca aatgataaac
     6121 caacagaaaa aacaaaagtt aaaaagaatg tttcacgagt caaagatatt aatcttcata
     6181 aaaaaggaaa tcctattacc ccaaaaaaaa taaaaaattt cagtcaacaa caattacgtt
     6241 tagcttctgg tgtttcaaat gaaaaaagag caataattac tcttaaaaaa taataaaact
     6301 ttatttaatt aaaaaaataa ttaaaaagaa aattaaatat atattaattt ttaaattatt
     6361 tgaaatgttt ttttcttgag ttactgactt ttccataccg ttatttgagc catcgagctt
     6421 tggattgtaa attgttgata aaaattcaga atcagatgca ccatactttg gagtacactt
     6481 tgttgtttta aaatcaagat tgttttgaat gttttcattc ttacaatagt ggcacaatgg
     6541 atctgtacat gtaaatctat tggatttctc ttgtgtaatg tttgtaatat gatatacatt
     6601 ttcaaatttt gaacaaatgt tgaatttttc aataattgtt ttactatttt ttgtacaatc
     6661 cattgaaaga gaagtaattt caacaaagct gtcttccact ctaaacaaat aaccatcatc
     6721 agtttcatta tattctttat aaagaataga ctctccaaac tcgtcattaa tacattcatt
     6781 aagtttaatt aagtttgtta aattacgaaa attgttacat cttgaattat ttgtttcaat
     6841 gtcatcataa aaaagcgcat gagtactatt tgtttcaact cttccatgaa tattacattc
     6901 atcataaatt acggcgcctg tcattgactt ttcaacacag ttttcttttt gatacataac
     6961 aataagaagt tgaaaatatt ttacttcaat ctgatcattt tgaagaacag aagattttga
     7021 tacaataaaa aaaaaaagaa aaaataataa cgttgttgag tacattatat acaatgaaaa
     7081 taataaatga acaaaaaata attttttatt ataatatatt aaaccccacc ccacaaccac
     7141 acctaaaaaa aagttaatac ttttttaaat ataatttttt taatagttat tttaatacgt
     7201 ttttaacata aaatatattt taattttttt tttaattttt tagaaaattt ttttttcata
     7261 attattttaa tactttttta caaaaaatat ttttaaaatt ttaaaaattt tttttttata
     7321 attattttaa tagcttaatt acaaaaacaa tttaataatt tttttaattt ttttttcata
     7381 atatttttaa tagctttttt acaaattatt tttacgaaaa tttttgttat atttttttat
     7441 aattatttta atacattttt aacattaaat aattatataa ttttttaata caataattac
     7501 agttataaat tcatattatt taaatatttt taaatgagtt tatttagaaa ttaagtttat
     7561 attttattta attagttttg actctcagaa tgtatagaca ctttttagaa cactgtgcac
     7621 aaagatgaag tttacattct tggttcaaat tacatttagt tccgttctta ctacacatgg
     7681 cacacttctt gttgattacc gaatcattga aacatggagg gcaaagttta gaattaataa
     7741 caacattata tatgtttatg tttattccta aacaattata gcaaatattg ttttgacatc
     7801 catctcttgt acatgaaata tataaatttt ttccttgaca caaagaatga caattaatac
     7861 actttacttc taagagagaa taattttctg gatttgaaag ggcatctgtt gaagaaacta
     7921 aaaagaaatc aattttggaa gaagttttat tgacttcttc tttaataaac ttttttaaat
     7981 cggcaatttc attggaattt atttgggtag attcggattg aattttgtaa taattgctat
     8041 ttactttttc gaaatcgat
//
