LOCUS       V007974                 6431 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V007974
VERSION     V007974
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Veltman DM, Akar G, Bosgraaf L, Van Haastert PJ.
  TITLE     A new set of small, extrachromosomal expression vectors for
            Dictyostelium discoideum
  JOURNAL   Plasmid 61 (2), 110-118 (2009)
   PUBMED   19063918
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Veltman DM, Akar G, Bosgraaf L, van Haastert PJM.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2008) Cell Biochemistry, Rijks Universiteit
            Groningen, Kerklaan 30, Haren 9751 NN, The Netherlands
REFERENCE   3  (bases 1 to 6431)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6431)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2009"; volume: "61"; issue: "2"; pages: "110-118"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-JUL-2008) Cell Biochemistry, Rijks Universiteit Groningen,
            Kerklaan 30, Haren 9751 NN, The Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      14..303
                     /label="act15 promoter"
                     /note="act15 promoter"
                     /regulatory_class="promoter"
     regulatory      332..594
                     /label="act8 terminator"
                     /note="act8 terminator"
                     /regulatory_class="terminator"
     CDS             606..1091
                     /codon_start=1
                     /product="D1'"
                     /label="D1'"
                     /protein_id="ACH81556.1"
                     /translation="FEKVNSNYYKIQSESTQINSNEIADLKKFIKEEVNKTSSKIDFFL
                     VSSTDALSNPENYSLLEVKCINCHSLCQGKNLYISCTRDGCQNNICYNCLGININIYNV
                     VINSKLCPPCFNDSVINKKCAMCSKNGTKCNLNQECKLHLCAQCSKKCLYILRVKTN"
     CDS             1594..2358
                     /codon_start=1
                     /product="D5"
                     /label="D5"
                     /protein_id="ACH81557.1"
                     /translation="MYSTTLLFFLFFFIVSKSSVLQNDQIEVKYFQLLIVMYQKENCVE
                     KSMTGAVIYDECNIHGRVETNSTHALFYDDIETNNSRCNNFRNLTNLIKLNECINDEFG
                     ESILYKEYNETDDGYLFRVEDSFVEITSLSMDCTKNSKTIIEKFNICSKFENVYHITNI
                     TQEKSNRFTCTDPLCHYCKNENIQNNLDFKTTKCTPKYGASDSEFLSTIYNPKLDGSNN
                     GMEKSVTQEKNISNNLKINIYLIFFLIIFLIK"
     CDS             complement(2366..2959)
                     /codon_start=1
                     /product="G5"
                     /label="G5"
                     /protein_id="ACH81558.1"
                     /translation="MFNHIKDSGYYATNEEIEIFLESCTLCKEITAQTKRNSYKKRNII
                     NKLPEEEEEEEEEEEEEEEEEEQEEEVEKPTISEEEEEETPAVSEEEKEEEEQEEDKEK
                     DKEKKIEEDTETGKKKDEVMNERQDGIEISEKTNDKPTEKTKVKKNVSRVKDINLHKKG
                     NPITPKKIKNFSQQQLRLASGVSNEKRAIITLKK"
     rep_origin      2869..3411
                     /label="Ddp1 ori"
                     /note="Ddp1 ori"
     rep_origin      complement(3742..4330)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4504..5361)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5362..5466)
                     /label="AmpR promoter"
     regulatory      complement(5513..5768)
                     /label="mhcA terminator"
                     /note="mhcA terminator"
                     /regulatory_class="terminator"
     CDS             complement(5778..6197)
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     regulatory      complement(6209..6430)
                     /label="act6 promoter"
                     /note="act6 promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 ctcgagacta gagctagata aaaaaaattt ttatttattt ttatttattt tgaattaaat
       61 agattacaaa ttaattaatc ccatcaaatc tttaaaaaaa aatggtttaa aaaaacttgg
      121 gttggttaat tattatttga aaattttaaa acccaaatta aaaaaaaaaa atgggattca
      181 aaaatttttt tttttttttt tttttttttt tttttttttt tttcagattg cataaaaaga
      241 tttttttttt ttttttttct tatttcttaa aacaaataaa ttaaattaaa taaaaaataa
      301 aaatcagatc tagtactagt taaataaata aattatttaa taaataataa aaaaacaaat
      361 tgttgtaata atctaatatt ttcttttttt tttaattttt tttttttaaa tcttaataat
      421 tattaagtta ttttaatttt tttttttttt tttttttttt tttttttttt ttttctatca
      481 aaaaaatcaa atatatttaa aaaatttatt atttacagat acattttgaa tggtgaagat
      541 aaatatatgc attagatgta aaacagccaa agagtatgaa aatcaaaaag ataaagctta
      601 tcgatttcga aaaagtaaat agcaattatt acaaaattca atccgaatct acccaaataa
      661 attccaatga aattgccgat ttaaaaaagt ttattaaaga agaagtcaat aaaacttctt
      721 ccaaaattga tttcttttta gtttcttcaa cagatgccct ttcaaatcca gaaaattatt
      781 ctctcttaga agtaaagtgt attaattgtc attctttgtg tcaaggaaaa aatttatata
      841 tttcatgtac aagagatgga tgtcaaaaca atatttgcta taattgttta ggaataaaca
      901 taaacatata taatgttgtt attaattcta aactttgccc tccatgtttc aatgattcgg
      961 taatcaacaa gaagtgtgcc atgtgtagta agaacggaac taaatgtaat ttgaaccaag
     1021 aatgtaaact tcatctttgt gcacagtgtt ctaaaaagtg tctatacatt ctgagagtca
     1081 aaactaatta aataaaatat aaacttaatt tctaaataaa ctcatttaaa aatatttaaa
     1141 taatatgaat ttataactgt aattattgta ttaaaaaatt atataattat ttaatgttaa
     1201 aaatgtatta aaataattat aaaaaaatat aacaaaaatt ttcgtaaaaa taatttgtaa
     1261 aaaagctatt aaaaatatta tgaaaaaaaa attaaaaaaa ttattaaatt gtttttgtaa
     1321 ttaagctatt aaaataatta taaaaaaaaa atttttaaaa ttttaaaaat attttttgta
     1381 aaaaagtatt aaaataatta tgaaaaaaaa attttctaaa aaattaaaaa aaaaattaaa
     1441 atatatttta tgttaaaaac gtattaaaat aactattaaa aaaattatat ttaaaaaagt
     1501 attaactttt ttttaggtgt ggttgtgggg tggggtttaa tatattataa taaaaaatta
     1561 ttttttgttc atttattatt ttcattgtat ataatgtact caacaacgtt attatttttt
     1621 cttttttttt ttattgtatc aaaatcttct gttcttcaaa atgatcagat tgaagtaaaa
     1681 tattttcaac ttcttattgt tatgtatcaa aaagaaaact gtgttgaaaa gtcaatgaca
     1741 ggcgccgtaa tttatgatga atgtaatatt catggaagag ttgaaacaaa tagtactcat
     1801 gcgctttttt atgatgacat tgaaacaaat aattcaagat gtaacaattt tcgtaattta
     1861 acaaacttaa ttaaacttaa tgaatgtatt aatgacgagt ttggagagtc tattctttat
     1921 aaagaatata atgaaactga tgatggttat ttgtttagag tggaagacag ctttgttgaa
     1981 attacttctc tttcaatgga ttgtacaaaa aatagtaaaa caattattga aaaattcaac
     2041 atttgttcaa aatttgaaaa tgtatatcat attacaaaca ttacacaaga gaaatccaat
     2101 agatttacat gtacagatcc attgtgccac tattgtaaga atgaaaacat tcaaaacaat
     2161 cttgatttta aaacaacaaa gtgtactcca aagtatggtg catctgattc tgaattttta
     2221 tcaacaattt acaatccaaa gctcgatggc tcaaataacg gtatggaaaa gtcagtaact
     2281 caagaaaaaa acatttcaaa taatttaaaa attaatatat atttaatttt ctttttaatt
     2341 atttttttaa ttaaataaag ttttattatt ttttaagagt aattattgct cttttttcat
     2401 ttgaaacacc agaagctaaa cgtaattgtt gttgactgaa attttttatt ttttttgggg
     2461 taataggatt tcctttttta tgaagattaa tatctttgac tcgtgaaaca ttctttttaa
     2521 cttttgtttt ttctgttggt ttatcatttg ttttttcact aatttcaata ccatcttgac
     2581 gttcattcat aacttcatct tttttttttc ctgtttctgt atcttcttct attttttttt
     2641 ctttatcttt ttctttatct tcttcttgtt cttcctcttc tttttcttct tctgatactg
     2701 caggtgtttc ttcttcttct tcttccgata ttgtcggttt ttctacttct tcttcttgtt
     2761 cttcttcttc ttcttcttct tcttcttctt cttcttcttc ttcttcttct tccggtaatt
     2821 tattaattat atttcttttt ttatatgaat tacgtttggt ttgtgcagta atttccttac
     2881 atagagtgca gctttcaaga aaaatttcaa tttcttcgtt tgttgcataa taaccactgt
     2941 ctttgatatg attaaacatt tttgattttc ttaaatgctt tccttcttta atatgaaaat
     3001 tatcgaattc taattcatta agaacaataa gctcccctaa tttaaaaaat tagttaaaat
     3061 aaattaaaat gaacatgtat aaagatggat tttaccattt tttgaaattc taaataactt
     3121 ttcttcatct ccaatctttt tgactgaaaa acgattttta attgaagtta ttgttctgtg
     3181 agtgttttga atcgcccatt tctctaaatc agtttgagat agtgttttat aatctgaatt
     3241 gttatacaca acttttgctc tattaaccaa atatttaaag atttcatcat caactgaata
     3301 ttttgacttt acgattcttg tccaaaaaac aatttctact actatcattt tttatttata
     3361 aaataattta aatacaaaaa tgaatttttt tttttttaaa aaaaaaaaaa tttgaaaaaa
     3421 aaaaaaaaaa aattttaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa tcaaataaaa
     3481 agtaaaaaat aaaaaccgaa aaacattcat tgtaatttca aatgtcgagg ccggcagagg
     3541 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4141 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     4201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4381 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4441 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4501 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4561 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4621 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4681 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4741 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4801 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4861 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4921 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4981 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5041 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5101 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5161 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5221 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5281 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5341 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5401 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5461 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggga tccattttat
     5521 ttaatatact aaataataaa aaagttaaaa aatgatcatt ggataaattt tttataatta
     5581 taaataaaga taataatttt ttttttaaca aaactaaaaa taaaaataat aaaataattg
     5641 ttaaaatagg tttttttttt tttttttttt ttttaataaa tggtatttat taatttattt
     5701 gttgtgtgtg tttttttttt tataatattt ttttttttag cattgaatta agaagaaatc
     5761 aaattgattc tagtttaatt tcgggtatat ttgagtggaa tgagttcttc aatcgtagtt
     5821 ttgactaact tgccattcat ttctattaac acaaaacaat ctggtgcata gtctgaaatc
     5881 aactccctac acataccaca aggacttacc actcgaatac ttctatctac ttcgtcagaa
     5941 taagggtgtc taacagctac aatcgtgtca aaatcctttt gtccattcga aactgcacta
     6001 ccaatcgcaa tggcttctgc acaaacagtt actcgtccta tatacgcttc aatatgtact
     6061 gccgaaatga tttctcctgt tttcgtacga attgccgctc ccacatgatg tttattatcc
     6121 tcataaagca ttgtaatctt ctctgtcgct acttctacta attctagatc ctgttgagaa
     6181 atgttaaatt gatccatttt ggatctttta tattatattt atttattgat tatttttttg
     6241 aattaattaa aaaaaaaaaa aatttcattt tataatctca gaaacctcaa aaaaaaaaaa
     6301 ataaaaaata aaaaatataa aaaaataaaa ataaaatccc aattttaaag cgaaaaacca
     6361 cccatggttt gaaaatttca atcaatttca aataacttta cttaaaaaaa acccattttt
     6421 tatttaaaaa a
//
