LOCUS       40924_14830        6878 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pDM320, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Veltman DM, Akar G, Bosgraaf L, Van Haastert PJ.
  TITLE     A new set of small, extrachromosomal expression vectors for 
            Dictyostelium discoideum
  JOURNAL   Plasmid 61 (2), 110-118 (2009)
  PUBMED    19063918
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Veltman DM, Akar G, Bosgraaf L, van Haastert PJM.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2008) Cell Biochemistry, Rijks Universiteit 
            Groningen, Kerklaan 30, Haren 9751 NN, The Netherlands
REFERENCE   3  (bases 1 to 6878)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6878)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2009"; volume: "61"; issue: "2"; pages: "110-118"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JUL-2008) Cell Biochemistry, Rijks Universiteit Groningen, 
            Kerklaan 30, Haren 9751 NN, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      14..303
                     /label=act15 promoter
                     /note="act15 promoter"
                     /regulatory_class="promoter"
     CDS             321..344
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     regulatory      371..633
                     /label=act8 terminator
                     /note="act8 terminator"
                     /regulatory_class="terminator"
     CDS             645..1130
                     /codon_start=1
                     /product="D1'"
                     /label=D1'
                     /protein_id="ACH81551.1"
                     /translation="FEKVNSNYYKIQSESTQINSNEIADLKKFIKEEVNKTSSKIDFFL
                     VSSTDALSNPENYSLLEVKCINCHSLCQGKNLYISCTRDGCQNNICYNCLGININIYNV
                     VINSKLCPPCFNDSVINKKCAMCSKNGTKCNLNQECKLHLCAQCSKKCLYILRVKTN"
     CDS             1633..2397
                     /codon_start=1
                     /product="D5"
                     /label=D5
                     /protein_id="ACH81552.1"
                     /translation="MYSTTLLFFLFFFIVSKSSVLQNDQIEVKYFQLLIVMYQKENCVE
                     KSMTGAVIYDECNIHGRVETNSTHALFYDDIETNNSRCNNFRNLTNLIKLNECINDEFG
                     ESILYKEYNETDDGYLFRVEDSFVEITSLSMDCTKNSKTIIEKFNICSKFENVYHITNI
                     TQEKSNRFTCTDPLCHYCKNENIQNNLDFKTTKCTPKYGASDSEFLSTIYNPKLDGSNN
                     GMEKSVTQEKNISNNLKINIYLIFFLIIFLIK"
     CDS             complement(2405..2998)
                     /codon_start=1
                     /product="G5"
                     /label=G5
                     /protein_id="ACH81553.1"
                     /translation="MFNHIKDSGYYATNEEIEIFLESCTLCKEITAQTKRNSYKKRNII
                     NKLPEEEEEEEEEEEEEEEEEEQEEEVEKPTISEEEEEETPAVSEEEKEEEEQEEDKEK
                     DKEKKIEEDTETGKKKDEVMNERQDGIEISEKTNDKPTEKTKVKKNVSRVKDINLHKKG
                     NPITPKKIKNFSQQQLRLASGVSNEKRAIITLKK"
     rep_origin      2908..3450
                     /label=Ddp1 ori
                     /note="Ddp1 ori"
     rep_origin      complement(3781..4369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4543..5400)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5401..5505)
                     /label=AmpR promoter
     regulatory      complement(5552..5807)
                     /label=mhcA terminator
                     /note="mhcA terminator"
                     /regulatory_class="terminator"
     CDS             complement(5817..6608)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(6656..6877)
                     /label=act6 promoter
                     /note="act6 promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 ctcgagacta gagctagata aaaaaaattt ttatttattt ttatttattt tgaattaaat
       61 agattacaaa ttaattaatc ccatcaaatc tttaaaaaaa aatggtttaa aaaaacttgg
      121 gttggttaat tattatttga aaattttaaa acccaaatta aaaaaaaaaa atgggattca
      181 aaaatttttt tttttttttt tttttttttt tttttttttt tttcagattg cataaaaaga
      241 tttttttttt ttttttttct tatttcttaa aacaaataaa ttaaattaaa taaaaaataa
      301 aaatcagatc caaaaaaatg gattataaag atgatgatga taaaagatct agtactagtt
      361 aaataaataa attatttaat aaataataaa aaaacaaatt gttgtaataa tctaatattt
      421 tctttttttt ttaatttttt ttttttaaat cttaataatt attaagttat tttaattttt
      481 tttttttttt tttttttttt tttttttttt tttctatcaa aaaaatcaaa tatatttaaa
      541 aaatttatta tttacagata cattttgaat ggtgaagata aatatatgca ttagatgtaa
      601 aacagccaaa gagtatgaaa atcaaaaaga taaagcttat cgatttcgaa aaagtaaata
      661 gcaattatta caaaattcaa tccgaatcta cccaaataaa ttccaatgaa attgccgatt
      721 taaaaaagtt tattaaagaa gaagtcaata aaacttcttc caaaattgat ttctttttag
      781 tttcttcaac agatgccctt tcaaatccag aaaattattc tctcttagaa gtaaagtgta
      841 ttaattgtca ttctttgtgt caaggaaaaa atttatatat ttcatgtaca agagatggat
      901 gtcaaaacaa tatttgctat aattgtttag gaataaacat aaacatatat aatgttgtta
      961 ttaattctaa actttgccct ccatgtttca atgattcggt aatcaacaag aagtgtgcca
     1021 tgtgtagtaa gaacggaact aaatgtaatt tgaaccaaga atgtaaactt catctttgtg
     1081 cacagtgttc taaaaagtgt ctatacattc tgagagtcaa aactaattaa ataaaatata
     1141 aacttaattt ctaaataaac tcatttaaaa atatttaaat aatatgaatt tataactgta
     1201 attattgtat taaaaaatta tataattatt taatgttaaa aatgtattaa aataattata
     1261 aaaaaatata acaaaaattt tcgtaaaaat aatttgtaaa aaagctatta aaaatattat
     1321 gaaaaaaaaa ttaaaaaaat tattaaattg tttttgtaat taagctatta aaataattat
     1381 aaaaaaaaaa tttttaaaat tttaaaaata ttttttgtaa aaaagtatta aaataattat
     1441 gaaaaaaaaa ttttctaaaa aattaaaaaa aaaattaaaa tatattttat gttaaaaacg
     1501 tattaaaata actattaaaa aaattatatt taaaaaagta ttaacttttt tttaggtgtg
     1561 gttgtggggt ggggtttaat atattataat aaaaaattat tttttgttca tttattattt
     1621 tcattgtata taatgtactc aacaacgtta ttattttttc tttttttttt tattgtatca
     1681 aaatcttctg ttcttcaaaa tgatcagatt gaagtaaaat attttcaact tcttattgtt
     1741 atgtatcaaa aagaaaactg tgttgaaaag tcaatgacag gcgccgtaat ttatgatgaa
     1801 tgtaatattc atggaagagt tgaaacaaat agtactcatg cgctttttta tgatgacatt
     1861 gaaacaaata attcaagatg taacaatttt cgtaatttaa caaacttaat taaacttaat
     1921 gaatgtatta atgacgagtt tggagagtct attctttata aagaatataa tgaaactgat
     1981 gatggttatt tgtttagagt ggaagacagc tttgttgaaa ttacttctct ttcaatggat
     2041 tgtacaaaaa atagtaaaac aattattgaa aaattcaaca tttgttcaaa atttgaaaat
     2101 gtatatcata ttacaaacat tacacaagag aaatccaata gatttacatg tacagatcca
     2161 ttgtgccact attgtaagaa tgaaaacatt caaaacaatc ttgattttaa aacaacaaag
     2221 tgtactccaa agtatggtgc atctgattct gaatttttat caacaattta caatccaaag
     2281 ctcgatggct caaataacgg tatggaaaag tcagtaactc aagaaaaaaa catttcaaat
     2341 aatttaaaaa ttaatatata tttaattttc tttttaatta tttttttaat taaataaagt
     2401 tttattattt tttaagagta attattgctc ttttttcatt tgaaacacca gaagctaaac
     2461 gtaattgttg ttgactgaaa ttttttattt tttttggggt aataggattt ccttttttat
     2521 gaagattaat atctttgact cgtgaaacat tctttttaac ttttgttttt tctgttggtt
     2581 tatcatttgt tttttcacta atttcaatac catcttgacg ttcattcata acttcatctt
     2641 ttttttttcc tgtttctgta tcttcttcta tttttttttc tttatctttt tctttatctt
     2701 cttcttgttc ttcctcttct ttttcttctt ctgatactgc aggtgtttct tcttcttctt
     2761 cttccgatat tgtcggtttt tctacttctt cttcttgttc ttcttcttct tcttcttctt
     2821 cttcttcttc ttcttcttct tcttcttctt ccggtaattt attaattata tttctttttt
     2881 tatatgaatt acgtttggtt tgtgcagtaa tttccttaca tagagtgcag ctttcaagaa
     2941 aaatttcaat ttcttcgttt gttgcataat aaccactgtc tttgatatga ttaaacattt
     3001 ttgattttct taaatgcttt ccttctttaa tatgaaaatt atcgaattct aattcattaa
     3061 gaacaataag ctcccctaat ttaaaaaatt agttaaaata aattaaaatg aacatgtata
     3121 aagatggatt ttaccatttt ttgaaattct aaataacttt tcttcatctc caatcttttt
     3181 gactgaaaaa cgatttttaa ttgaagttat tgttctgtga gtgttttgaa tcgcccattt
     3241 ctctaaatca gtttgagata gtgttttata atctgaattg ttatacacaa cttttgctct
     3301 attaaccaaa tatttaaaga tttcatcatc aactgaatat tttgacttta cgattcttgt
     3361 ccaaaaaaca atttctacta ctatcatttt ttatttataa aataatttaa atacaaaaat
     3421 gaattttttt ttttttaaaa aaaaaaaaat ttgaaaaaaa aaaaaaaaaa attttaaaaa
     3481 aaaaaaaaaa aaaaaaaaaa aaaaaaaaat caaataaaaa gtaaaaaata aaaaccgaaa
     3541 aacattcatt gtaatttcaa atgtcgaggc cggcagaggc ggtttgcgta ttgggcgctc
     3601 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3661 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3721 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3781 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3841 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3901 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3961 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4021 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4081 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4141 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4201 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     4261 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4321 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4381 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4441 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4501 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4561 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4621 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4681 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4741 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4801 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4861 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4921 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4981 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5041 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5101 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5161 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5221 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5281 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5341 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5401 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5461 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5521 agtgccacct gacgcgccct gtagcgggat ccattttatt taatatacta aataataaaa
     5581 aagttaaaaa atgatcattg gataaatttt ttataattat aaataaagat aataattttt
     5641 tttttaacaa aactaaaaat aaaaataata aaataattgt taaaataggt tttttttttt
     5701 tttttttttt tttaataaat ggtatttatt aatttatttg ttgtgtgtgt tttttttttt
     5761 ataatatttt tttttttagc attgaattaa gaagaaatca aattgattct agttcagaag
     5821 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     5881 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     5941 aacgctatgt cctgatagcg gtccgccaca cccagccgtc cacagtcgat gaatccagaa
     6001 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     6061 tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     6121 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     6181 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     6241 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     6301 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     6361 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     6421 tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     6481 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     6541 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     6601 tcaatcatgc gaaacgatcc agcttgaaca tcttcaccat ccattttgga tcttttatat
     6661 tatatttatt tattgattat ttttttgaat taattaaaaa aaaaaaaaat ttcattttat
     6721 aatctcagaa acctcaaaaa aaaaaaaata aaaaataaaa aatataaaaa aataaaaata
     6781 aaatcccaat tttaaagcga aaaaccaccc atggtttgaa aatttcaatc aatttcaaat
     6841 aactttactt aaaaaaaacc cattttttat ttaaaaaa
//
