LOCUS       40924_14820        3653 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pDM312, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Veltman DM, Akar G, Bosgraaf L, Van Haastert PJ.
  TITLE     A new set of small, extrachromosomal expression vectors for 
            Dictyostelium discoideum
  JOURNAL   Plasmid 61 (2), 110-118 (2009)
  PUBMED    19063918
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Veltman DM, Akar G, Bosgraaf L, van Haastert PJM.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2008) Cell Biochemistry, Rijks Universiteit 
            Groningen, Kerklaan 30, Haren 9751 NN, The Netherlands
REFERENCE   3  (bases 1 to 3653)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3653)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2009"; volume: "61"; issue: "2"; pages: "110-118"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JUL-2008) Cell Biochemistry, Rijks Universiteit Groningen, 
            Kerklaan 30, Haren 9751 NN, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             10..681
                     /codon_start=1
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /translation="ASSEDVIKEFMRFKVKMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERIMNFEDGGVV
                     TVTQDSTLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYKAKKQVQLPGAYKTDIKLDIISHNEDYTIVEQYERAEGRHST
                     GA"
     primer_bind     complement(703..719)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(745..763)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(773..789)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(930..1385)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1412..1516
                     /label=AmpR promoter
     CDS             1517..2374
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2548..3136
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3424..3445
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3460..3490
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3498..3514
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3522..3538
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3559..3577
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3614..3630
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 actagtagtg catcatcaga agatgttatt aaagaattta tgagattcaa agttaaaatg
       61 gaaggttcag ttaacggtca tgaatttgaa attgaaggtg aaggtgaagg tagaccatat
      121 gaaggtacac aaacagcaaa attaaaagtt acaaaaggtg gtccattacc atttgcatgg
      181 gatattcttt caccacaatt tcaatatggt agtaaagcat atgttaaaca tccagcagat
      241 attccagatt atcttaaact ttcatttcca gaaggtttta aatgggaaag aattatgaat
      301 tttgaagatg gtggtgttgt aacagttact caagattcaa cattacaaga tggtgaattt
      361 atttataaag ttaaattaag aggtaccaat tttccatcag atggtccagt aatgcaaaaa
      421 aaaactatgg gttgggaagc atcaacagaa agaatgtatc cagaagatgg tgcactaaaa
      481 ggtgaaatta aaatgagatt aaaacttaag gatggtggtc attatgatgc agaagttaaa
      541 acaacttata aagctaaaaa acaagttcaa ttaccaggtg cttataaaac agatattaaa
      601 ttagatatta tttcacataa tgaagattat actattgttg aacaatatga aagagcagaa
      661 ggtagacatt caacaggtgc ataatctaga gatcaagctt atcgataccg tcgacctcga
      721 gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
      781 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
      841 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
      901 aacagttgcg cagcctgaat ggcgaatgga aattgtaagc gttaatattt tgttaaaatt
      961 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     1021 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     1081 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     1141 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     1201 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     1261 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     1321 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     1381 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1441 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1501 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1561 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1621 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1681 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1741 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1801 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1861 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1921 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1981 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     2041 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     2101 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     2161 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     2221 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2281 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2341 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2401 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2461 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2521 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2581 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2641 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     2701 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2761 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2821 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2881 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2941 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3001 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3061 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3301 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     3361 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     3421 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     3481 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     3541 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctccaccg
     3601 cggtggcggc cgctctagaa ctagtggatc ccccgggctg caggaattcg atg
//
