LOCUS       40924_14800       12210 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pDM2L, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12210)
  AUTHORS   Chan CT, Lee JW, Cameron DE, Bashor CJ, Collins JJ.
  TITLE     'Deadman' and 'Passcode' microbial kill switches for bacterial 
            containment
  JOURNAL   Nat. Chem. Biol. 12 (2), 82-86 (2016)
  PUBMED    26641934
REFERENCE   2  (bases 1 to 12210)
  AUTHORS   Chan CTY., Lee JW, Cameron DE, Bashor CJ, Collins JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2015) Biological engineering, MIT, 45 Carleton St,
            MIT Building E25-Rm302, Cambridge, MA 02142, USA
REFERENCE   3  (bases 1 to 12210)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12210)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem. 
            Biol."; date: "2016"; volume: "12"; issue: "2"; pages: "82-86"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-OCT-2015) Biological engineering, MIT, 45 Carleton St, MIT 
            Building E25-Rm302, Cambridge, MA 02142, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(105..1073)
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             complement(1076..2248)
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    complement(2574..2824)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(2830..3582)
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     rep_origin      complement(3673..3892)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      complement(3968..4582)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        5440..5542
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             5543..6199
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      complement(6337..6431)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6494..7207)
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     RBS             7213..7221
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      complement(7249..7322)
                     /label=PLtetO promoter
                     /note="PLtetO promoter"
                     /regulatory_class="promoter"
     promoter        complement(7249..7322)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     misc_feature    7268
                     /label=transcription start site
                     /note="transcription start site"
     protein_bind    7304..7322
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    complement(7365..7448)
                     /label=ssrA tag (mf-lon)
                     /note="ssrA tag (mf-lon)"
     CDS             complement(7449..8528)
                     /label=lacI
                     /note="lac repressor"
     RBS             8534..8542
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(8564..8637)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     promoter        8669..8698
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     misc_feature    8704
                     /label=transcription start site
                     /note="transcription start site"
     protein_bind    8706..8722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             8755..8763
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8769..9389
                     /label=TetR
                     /note="tetracycline repressor TetR"
     regulatory      9397..9509
                     /label=rnpBT1 terminator
                     /note="rnpBT1 terminator"
                     /regulatory_class="terminator"
     protein_bind    9528..9547
                     /label=lac operator (symmetric)
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    9564..9583
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        9584..9613
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    complement(9620..9639)
                     /label=lac operator (symmetric)
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     RBS             9656..9664
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9668..12031
                     /codon_start=1
                     /product="protease"
                     /label=protease
                     /note="derived from Mesoplasma florum lon codon optimized"
                     /protein_id="ALP32215.1"
                     /translation="MSKKIKLPIFQIRGSFIVPGIKENLEVGRKNTLASVNYAIKNSNN
                     QMIAIPQIDASVEKPEFSDLHEFGILIDFEVIKEWKDNSLTISTNPIQRCKVISFFENE
                     DQVPYAEVELIESINDFSDEELKELIEKISDAIKTKASLVTKQIKQLISGESDDLSLAF
                     DSIMFKLAPSKILTNPEYITSPSLKTRWSIIEKIIFAEDGIITRNAESIDAARQKNEIE
                     QELNHKLKEKMDKQQKEYYLREKMRIIKDELEDEDDSDDSSLEKYKERLAKEPFPEEVK
                     RKIMASIKRVEALQSGTPEWNTEKNYIDWMMSIPWWEETEDLTDLKYAKKILDKHHYGM
                     KKVKERIIEYLAVKTKTKSLKAPIITLVGPPGVGKTSLAKSIAEAVGKNFVKVSLGGVK
                     DESEIRGHRKTYVGSMPGRIIQTMKRAKVKNPLFLLDEIDKMASDHRGDPASAMLEVLD
                     PEQNKEFSDHYIEEPYDLSQVMFIATANYPEDIPEALYDRMEIINLSSYTEIEKVKIAQ
                     DYLVPKAIEQHELTSEEISFTEGAINEIIKYYTREAGVRQLERHINSIIRKYIVKNLNG
                     EMDKIVIDEKQVNDLLGKRIFDHTEKQEESQIGVVTGLAYTQFGGDILPIEVSLYPGKG
                     NLILTGKLGEVMKESATIALTYVKSNFEKFGVDKKVFEENDIHVHVPEGAVPKDGPSAG
                     ITITTALISALSDKPVSKEIGMTGEITLRGNVLPIGGLREKSISASRSGLKTIIIPKKN
                     ERDLDEIPDEVKAKLKIIPAEKYEEVFAIVFKTK"
     terminator      12068..12154
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 aagtggggcc cagagagaat attcaggcca gttatgcttt ctggcctgta acaaaggaca
       61 ttaagtaaag acagataaac gtagactaaa acgtggtcgc atcagggtgc tggcttttca
      121 agttccttaa gaatggcctc aattttctct atacactcag ttggaacacg ggacctgtcc
      181 aggttaagca ccattttatc gcccttatac aatactgtcg ctccaggagc aaactgatgt
      241 cgtgagctta aactagttct tgatgcagat gacgttttaa gcacagaagt taaaagagtg
      301 ataacttctt cagcttcaaa tatcacccca gcttttttct gctcatgaag gttagatgcc
      361 tgctgcttaa gtaattcctc tttatctgta aaggcttttt gaagtgcatc acctgaccgg
      421 gcagatagtt caccggggtg agaaaaaaga gcaacaactg atttaggcaa tttggcggtg
      481 ttgatacagc gggtaataat cttacgtgaa atattttccg catcagccag cgcagaaata
      541 tttccagcaa attcattctg caatcggctt gcataacgct gaccacgttc ataagcactt
      601 gttgggcgat aatcgttacc caatctggat aatgcagcca tctgctcatc atccagctcg
      661 ccaaccagaa cacgataatc actttcggta agtgcagcag ctttacgacg gcgactccca
      721 tcggcaattt ctatgacacc agatactctt cgaccgaacg ccggtgtctg ttgaccagtc
      781 agtagaaaag aagggatgag atcatccagt gcgtcctcag taagcagctc ctggtcacgt
      841 tcattacctg accatacccg agaggtcttc tcaacactat caccccggag cacttcaaga
      901 gtaaacttca catcccgacc acatacaggc aaagtaatgg cattaccgcg agccattact
      961 cctacgcgcg caattaacga atccaccatc ggggcagctg gtgtcgataa cgaagtatct
     1021 tcaaccggtt gagtattgag cgtatgtttt ggaataacag gcgcacgctt cattatctaa
     1081 tctcccagcg tggtttaatc agacgatcga aaatttcatt gcagacaggt tcccaaatag
     1141 aaagagcatt tctccaggca ccagttgaag agcgttgatc aatggcctgt tcaaaaacag
     1201 ttctcatccg gatctgacct ttaccaactt catccgtttc acgtacaaca ttttttagaa
     1261 ccatgcttcc ccaggcatcc cgaatttgct cctccatcca cggggactga gagccattac
     1321 tattgctgta tttggtaagc aaaatacgta catcaggctc gaacccttta agatcaacgt
     1381 tcttgagcag atcacgaagc atatcgaaaa actgcagtgc ggaggtgtag tcaaacaact
     1441 cagcaggcgt gggaacaatc agcacatcag cagcacatac gacattaatc gtgccgatac
     1501 ccaggttagg cgcgctgtca ataactatga catcatagtc atgagcaaca gtttcaatgg
     1561 ccagtcggag catcaggtgt ggatcggtgg gcagtttacc ttcatcaaat ttgcccatta
     1621 actcagtttc aatacggtgc agagccagac aggaaggaat aatgtcaagc cccggccagc
     1681 aagtgggctt tattgcataa gtgacatcgt ccttttcccc aagatagaaa ggcaggagag
     1741 tgtcttctgc atgaatatga agatctggta cccatccgtg atacattgag gctgttccct
     1801 gggggtcgtt accttccacg agcaaaacac gtagcccctt cagagccaga tcctgagcaa
     1861 gatgaacaga aactgaggtt ttgtaaacgc cacctttatg ggcagcaacc ccgatcaccg
     1921 gtggaaatac gtcttcagca cgtcgcaatc gcgtaccaaa cacatcacgc atatgattaa
     1981 tttgttcgat tgtataacca acacgttgct caacccgtcc tcgaatttcc atatccgggt
     2041 gcggtagtcg ccctgctttc tcggcatctc tgatagcctg agaagaaacc ccaactaaat
     2101 ccgctgcttc acctattctc cagcgccggg ttattttcct cgcttccggg ctgtcatcat
     2161 taaactgtgc aatggcgata gccttcgtca tttcatgacc agcgtttatg cactggttaa
     2221 gtgtttccat gagtttcatt ctgaacatcc tttaatcatt gctttgcgtt tttttattaa
     2281 atcttgcaat ttactgcaaa gcaacaacaa aatcgcaaag tcatcaaaaa accgcaaagt
     2341 tgtttaaaat aagagcaaca ctacaaaagg agataagaag agcacatacc tcagtcactt
     2401 attatcacta gcgctcgccg cagccgtgta accgagcata gcgagcgaac tggcgaggaa
     2461 gcaaagaaga actgttctgt cagatagctc ttacgctcag cgcaagaaga aatatccacc
     2521 gtgggaaaaa ctccaggtag aggtacacac gcggatagcc aattcagagt aataaactgt
     2581 gataatcaac cctcatcaat gatgacgaac taacccccga tatcaggtca catgacgaag
     2641 ggaaagagaa ggaaatcaac tgtgacaaac tgccctcaaa tttggcttcc ttaaaaatta
     2701 cagttcaaaa agtatgagaa aatccatgca ggctgaagga aacagcaaaa ctgtgacaaa
     2761 ttaccctcag taggtcagaa caaatgtgac gaaccaccct caaatctgtg acagataacc
     2821 ctcagactat cctgtcgtca tggaagtgat atcgcggaag gaaaatacga tatgagtcgt
     2881 ctggcggcct ttctttttct caatgtatga gaggcgcatt ggagttctgc tgttgatctc
     2941 attaacacag acctgcagga agcggcggcg gaagtcaggc atacgctggt aactttgagg
     3001 cagctggtaa cgctctatga tccagtcgat tttcagagag acgatgcctg agccatccgg
     3061 cttacgatac tgacacaggg attcgtataa acgcatggca tacggattgg tgatttcttt
     3121 tgtttcacta agccgaaact gcgtaaaccg gttctgtaac ccgataaaga agggaatgag
     3181 atatgggttg atatgtacac tgtaaagccc tctggatgga ctgtgcgcac gtttgataaa
     3241 ccaaggaaaa gattcatagc ctttttcatc gccggcatcc tcttcagggc gataaaaaac
     3301 cacttccttc cccgcgaaac tcttcaatgc ctgccgtata tccttactgg cttccgcaga
     3361 ggtcaatccg aatatttcag catatttagc aacatggatc tcgcagatac cgtcatgttc
     3421 ctgtagggtg ccatcagatt ttctgatctg gtcaacgaac agatacagca tacgtttttg
     3481 atcccgggag agactatatg ccgcctcagt gaggtcgttt gactggacga ttcgcgggct
     3541 atttttacgt ttcttgtgat tgataaccgc tgtttccgcc atgacagatc catgtgaagt
     3601 gtgacaagtt tttagattgt cacactaaat aaaaaagagt caataagcag ggataacttt
     3661 gtgaaaaaac agcttcttct gagggcaatt tgtcacaggg ttaagggcaa tttgtcacag
     3721 acaggactgt catttgaggg tgatttgtca cactgaaagg gcaatttgtc acaacacctt
     3781 ctctagaacc agcatggata aaggcctaca aggcgctcta aaaaagaaga tctaaaaact
     3841 ataaaaaaaa taattataaa aatatccccg tggataagtg gataacccca agggaagttt
     3901 tttcaggcat cgtgtgtaag cagaatatat aagtgctgtt ccctggtgct tcctcgctca
     3961 ctcgaccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca
     4021 cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa
     4081 gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc
     4141 tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc
     4201 aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg
     4261 cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc
     4321 gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc
     4381 ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca
     4441 tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc cggccgcggt
     4501 gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca
     4561 gcccagcggc gagggcaacc agccgagggc ttcgccctgt cgctcgactg cggcgagcac
     4621 tactggctgt aaaaggacag accacatcat ggttctgtgt tcattaggtt gttctgtcca
     4681 ttgctgacat aatccgctcc acttcaacgt aacaccgcac gaagatttct attgttcctg
     4741 aaggcatatt caaatcgttt tcgttaccgc ttgcaggcat catgacagaa cactacttcc
     4801 tataaacgct acacaggctc ctgagattaa taatgcggat ctctacgata atgggagatt
     4861 ttcccgactg tttcgttcgc ttctcagtgg ataacagcca gcttctctgt ttaacagaca
     4921 aaaacagcat atccactcag ttccacattt ccatataaag gccaaggcat ttattctcag
     4981 gataattgtt tcagcatcgc aaccgcatca gactccggca tcgcaaactg cacccggtgc
     5041 cgggcagcca catccagcgc aaaaaccttc gtgtagactt ccgttgaact gatggactta
     5101 tgtcccatca ggctttgcag aactttcagc ggtataccgg catacagcat gtgcatcgca
     5161 taggaatggc ggaacgtatg tggtgtgacc ggaacagaga acgtcacacc gtcagcagca
     5221 gcggcggcaa ccgcctcccc aatccaggtc ctgaccgttc tgtccgtcac ttcccagatc
     5281 cgcgctttct ctgtccttcc tgtgcgacgg ttacgccgct ccatgagctt atcgcgaata
     5341 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
     5401 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
     5461 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
     5521 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     5581 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     5641 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     5701 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     5761 tcgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     5821 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     5881 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     5941 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     6001 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcacgat
     6061 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     6121 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     6181 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
     6241 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga tgataagctg
     6301 tcaaacatga gaattggtct cgacgagtat gcttggattc tcaccaataa aaaacgcccg
     6361 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     6421 caacaggagt ccaagcgacg tctgtgcaag tactactgtt ctgcagtcac ttgaattcga
     6481 tacccagctg ttatttgtat agttcatcca tgccatgtgt aatcccagca gcggttacaa
     6541 actcaagaag gaccatgtgg tctctctttt cgttgggatc tttcgaaagg gcagattgtg
     6601 tggacaggta atggttgtct ggtaaaagga cagggccatc gccaatcgga gtattttgtt
     6661 gataatggtc tgctagttga acgcttccat cttcaatgtt gtgtctaatt ttgaagttaa
     6721 ctttgattcc attcttttgt ttgtctgcca tgatgtatac attgtgtgag ttatagttgt
     6781 attccaattt gtgtccaaga atgtttccat cttctttaaa atcaatacct tttaactcga
     6841 ttctattaac aagggtatca ccttcaaact tgacttcagc acgtgtcttg tagttcccgt
     6901 catctttgaa aaatatagtt ctttcctgta cgtaaccttc gggcatggca ctcttgaaaa
     6961 agtcatgctg tttcatgtga tctgggtatc tcgcaaagca ttgaacacca taaccgaaag
     7021 tagtgacaag tgttggccac ggaacaggta gttttccagt agtgcaaata aatttaaggg
     7081 taagttttcc gtatgttgca tcaccttcac cctctccact gacagaaaat ttgtgcccat
     7141 taacatcacc atctaattca acaagaattg ggacaactcc agtgaaaagt tcttctcctt
     7201 tacgcatatt atacctcctt aatttagtgt catctacaac tggcatgcgg tcagtgcgtc
     7261 ctgctgatgt gctcagtata tctatcactg atagggatgt caatctctat cactgatagg
     7321 gaaacgtttc gcagaagctt ccgcaaggta ccactttgcc gcggttattg agacaggtag
     7381 ttcgcctggc ccgcgttcag cataaaggtc ggcacttcgt tggtgttttc ttcgtttttg
     7441 ttcgccgcct gcccgctttc cagtcgggaa acctgtcgtg ccagttgcat taatgaatcg
     7501 gccaacgcgc ggggagaggc ggtttgcgta ttgggagcca gggtggtttt tcttttcacc
     7561 agtgagacgg gcaacagttg attgcccttc accgcctggc cctgagagag ttgcagcaag
     7621 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
     7681 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg
     7741 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
     7801 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
     7861 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
     7921 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
     7981 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
     8041 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
     8101 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
     8161 atcagcccac tgacgcgctg cgcgagaaga ttgtgaaccg ccgctttaca ggcttcgacg
     8221 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
     8281 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
     8341 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
     8401 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
     8461 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta cagcgttact
     8521 ggtttcatat tgtacctcct taattcttga ttttctatcc tccggtcagt gcgtcctgct
     8581 gatgtgctca gtatatctat cactgatagg gatgtcaatc tctatcactg atagggacgg
     8641 ccgcccgttc catggctgaa atgagctgtt gacaattaat catccggctc gtataatgtg
     8701 tggaattgtg agcggataac aatttcacac gtcgacgcac cataaagcgg aaagtaagga
     8761 ggttagatat gtctcgttta gataaaagta aagtgattaa cagcgcatta gagctgctta
     8821 atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc
     8881 agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg
     8941 agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt
     9001 ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa
     9061 aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag
     9121 cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg
     9181 ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa
     9241 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg
     9301 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcatc tgcggattag
     9361 aaaaacaact taaatgtgaa agtgggtctt gataatccgg cttatcggtc agtttcacct
     9421 gatttacgta aaaacccgct tcggcgggtt tttgcttttg gaggggcaga aagatgaatg
     9481 actgtccacg acgctatacc caaaagaaag agctcggact ggactcgaat tgtgagcgct
     9541 cacaattact agcggccgca tggaattgtg agcgctcaca attttgacaa ttaatcatcc
     9601 ggctcgtata atgtgtggga attgtgagcg ctcacaattt cacacgaatt cgcattaagg
     9661 aggtaccatg agtaaaaaaa tcaaactacc tattttccaa atccgtggtt ctttcatcgt
     9721 tccgggtatc aaagaaaacc tggaagttgg tcgtaaaaac accctggctt ctgttaacta
     9781 cgctatcaaa aactctaaca accagatgat cgctatcccg cagatcgacg cttctgttga
     9841 aaaaccggag ttctctgacc tgcacgagtt cggtatcctg atcgacttcg aagttatcaa
     9901 agaatggaaa gacaactctc tgaccatctc taccaacccg atccagcgtt gcaaagttat
     9961 ctctttcttc gaaaacgaag accaggttcc gtacgctgaa gttgaactga tcgaatctat
    10021 caacgacttc tctgacgaag aactgaaaga actgatcgaa aaaatctctg acgctatcaa
    10081 aaccaaggct tctctggtta ccaaacagat caaacaactg atctctggtg aatctgacga
    10141 cctgtctctg gctttcgact ctatcatgtt caaactggct ccgtctaaaa tcctgaccaa
    10201 cccggaatac atcacctctc cgtctctgaa aacccgttgg tctatcatcg aaaaaatcat
    10261 cttcgctgaa gacggtatca tcacccgtaa cgctgaatct atcgacgctg ctcgtcagaa
    10321 aaacgaaatc gaacaggaac tgaaccacaa actgaaagaa aaaatggaca aacagcagaa
    10381 agaatactac ctgcgtgaaa aaatgcgtat catcaaagac gaactggaag acgaagacga
    10441 ctctgacgac tcttctctgg aaaaatacaa agaacgtctg gctaaagaac cgttcccgga
    10501 agaagttaaa cgtaaaatca tggcttctat caaacgtgtt gaagctctgc aatctggaac
    10561 cccggaatgg aacaccgaaa aaaactacat cgactggatg atgtctatcc cgtggtggga
    10621 agaaaccgaa gacctgaccg acctgaaata cgctaaaaaa atcctggaca aacaccacta
    10681 cggtatgaaa aaagttaaag aacgtatcat cgaatacctg gctgttaaaa ccaaaaccaa
    10741 atctctgaaa gctccgatca tcaccctggt tggtccgccg ggtgttggta aaacctctct
    10801 ggctaaatct atcgctgaag ctgttggtaa aaacttcgtt aaagtttctc tgggtggtgt
    10861 taaagacgaa tctgaaatcc gtggtcaccg taaaacctac gttggttcta tgccgggtcg
    10921 tatcatccag accatgaaac gtgctaaagt taaaaacccg ctgttcctgc tggacgaaat
    10981 cgacaaaatg gcttctgacc accgtggtga cccggcttct gctatgctgg aagttctgga
    11041 cccggaacag aacaaagagt tctctgacca ctacatcgaa gaaccgtacg acctgtctca
    11101 ggttatgttc atcgctaccg ctaactaccc ggaagacatc ccggaagctc tgtacgaccg
    11161 tatggaaatc atcaacctgt cttcttacac cgaaatcgaa aaagttaaaa tcgctcagga
    11221 ctacctggtt ccgaaagcta tcgaacagca cgaactgacc tctgaagaaa tctctttcac
    11281 cgaaggtgct atcaacgaaa tcatcaaata ctacacccgt gaagctggtg ttcgtcaact
    11341 ggaacgtcac atcaactcta tcatccgtaa atacatcgtt aaaaacctga acggtgaaat
    11401 ggacaaaatc gttatcgacg aaaaacaggt taacgacctg ctgggtaaac gtatcttcga
    11461 ccacaccgaa aaacaggaag aatctcagat cggtgttgtt accggtctgg cttacaccca
    11521 gttcggtggt gacatcctgc cgattgaagt ttctctgtat ccgggtaaag gtaacctgat
    11581 cctgaccggt aaactgggtg aagttatgaa agaatctgct accatcgctc tgacctacgt
    11641 taaatctaac ttcgaaaaat tcggtgttga caaaaaagtt ttcgaagaaa acgacatcca
    11701 cgttcacgtt ccggaaggtg ctgttccgaa agacggtccg tctgctggta tcaccatcac
    11761 caccgctctg atctctgctc tgtctgacaa accggtttct aaagaaatcg gtatgaccgg
    11821 tgaaatcacc ctgcgtggta acgttctgcc gattggtggt ctgcgtgaaa aatctatctc
    11881 tgcttctcgt tctggtctga aaaccatcat catcccgaaa aaaaacgaac gtgacctgga
    11941 cgaaatcccg gacgaagtta aagctaaact gaaaatcatc ccggctgaaa aatacgaaga
    12001 ggtatttgca atagttttta aaacaaaata atctcgagag aatcaatatt aatccaacgc
    12061 gtggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
    12121 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ccctagactt aggcgttcgg
    12181 ctgcggcgag cggtatcagc tcactcaaag
//
