LOCUS       40924_14775        3666 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pDM131, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Veltman DM, Akar G, Bosgraaf L, Van Haastert PJ.
  TITLE     A new set of small, extrachromosomal expression vectors for 
            Dictyostelium discoideum
  JOURNAL   Plasmid 61 (2), 110-118 (2009)
  PUBMED    19063918
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Veltman DM, Akar G, Bosgraaf L, van Haastert PJM.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2008) Cell Biochemistry, Rijks Universiteit 
            Groningen, Kerklaan 30, Haren 9751 NN, The Netherlands
REFERENCE   3  (bases 1 to 3666)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3666)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2009"; volume: "61"; issue: "2"; pages: "110-118"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JUL-2008) Cell Biochemistry, Rijks Universiteit Groningen, 
            Kerklaan 30, Haren 9751 NN, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             12..686
                     /codon_start=1
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /translation="MASSEDVIKEFMRFKVKMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERIMNFEDGGV
                     VTVTQDSTLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYKAKKQVQLPGAYKTDIKLDIISHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(726..742)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(779..797)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(818..834)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(842..858)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(866..896)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(911..932)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1220..1808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1982..2839)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2840..2944)
                     /label=AmpR promoter
     rep_origin      2971..3426
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3567..3583
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3593..3611
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3637..3653
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggatccaaaa aatggcatca tcagaagatg ttattaaaga atttatgaga ttcaaagtta
       61 aaatggaagg ttcagttaac ggtcatgaat ttgaaattga aggtgaaggt gaaggtagac
      121 catatgaagg tacacaaaca gcaaaattaa aagttacaaa aggtggtcca ttaccatttg
      181 catgggatat tctttcacca caatttcaat atggtagtaa agcatatgtt aaacatccag
      241 cagatattcc agattatctt aaactttcat ttccagaagg ttttaaatgg gaaagaatta
      301 tgaattttga agatggtggt gttgtaacag ttactcaaga ttcaacatta caagatggtg
      361 aatttattta taaagttaaa ttaagaggta ccaattttcc atcagatggt ccagtaatgc
      421 aaaaaaaaac tatgggttgg gaagcatcaa cagaaagaat gtatccagaa gatggtgcac
      481 taaaaggtga aattaaaatg agattaaaac ttaaggatgg tggtcattat gatgcagaag
      541 ttaaaacaac ttataaagct aaaaaacaag ttcaattacc aggtgcttat aaaacagata
      601 ttaaattaga tattatttca cataatgaag attatactat tgttgaacaa tatgaaagag
      661 cagaaggtag acattcaaca ggtgcaagat ctagtactag tgcatcgaat tcctgcagcc
      721 cgggggatcc actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc
      781 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      841 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      901 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      961 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1021 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1081 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1141 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1201 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1261 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1321 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1381 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1441 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1501 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1561 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1621 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     1681 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1741 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1801 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1861 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1921 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1981 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2041 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2101 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2161 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2221 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2281 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2341 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2401 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2461 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2521 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2581 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2641 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2701 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2761 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2821 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2881 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2941 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     3001 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3061 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3121 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3181 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3241 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3301 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     3361 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     3421 caatttccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     3481 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     3541 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     3601 ctcactatag ggcgaattgg gtaccgggcc ccccctcgag gtcgacggta tcgataagct
     3661 tgatgc
//
