LOCUS       62056_8485        8180 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    202..223
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        238..268
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    276..292
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     300..316
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             519..542
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     primer_bind     complement(1833..1849)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      2323..2409
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2501..2528
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(2824..2867)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      7356..7944
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaatacgttt cccatttagc cgtcatttca gcactcgtta aaaggtgctg actttcaact
       61 gcctggcaca ataattttcc tttttcagtt acaacaagct ccaatacgca aaccgcctct
      121 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
      181 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
      241 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
      301 aggaaacagc tatgaccatg attacgccaa gcttgaaaag ccctgacaac ccttgttcct
      361 aaaaaggaat aagcgttcgg tcagtaaata atagaaataa aaaatcagac ctaagactga
      421 tgacaaaaag agaaaatttt gataaaatag tcttagaatt aaattaaaaa gggaggccaa
      481 atataatgaa aaatatgaat gacaatgatg ttcatatgga ctacaaggac gacgatgaca
      541 agggaggtgg cggttcactc gaggtgaaaa aaaaattagt ctcatcactt ctaaagtgtt
      601 ctctaatcat tattgttagc tttgctggtg gagcatttgc tagttttgtc atgaatcata
      661 atgacaatat tccaaatggt ggtgtcacta aaactagtaa agtaaattat aataacataa
      721 cgcctacaac aaaagctgtt aaaaaggtac aaaatagtgt tgtttctgtt atcaattata
      781 aacaacaaga gagtcgttct gacctatcag acttctatag tcattttttt ggtaatcagg
      841 ggggcaacac tgataagggc ttacaagttt acggtgaagg ctctggagtc atctataaaa
      901 aagatggtaa aaatgcctat gttgtcacta ataaccacgt cattgatggg gctaaacaaa
      961 ttgaaattca actagctgat ggctcaaaag cagttgggaa acttgttggg tcagatacct
     1021 actctgattt agccgtcgtc aaaattccat cagataaggt ttcaaatatt gcagaatttg
     1081 ctgattcatc aaaactcaac attggtgaaa ctgctatagc gatcggaagc cctcttggaa
     1141 ctgagtatgc aaattctgta actcaaggta ttgtatctag tttaaaaaga actgtaacaa
     1201 tgactaatga agaaggacaa acagtttcta caaatgctat ccagactgat gctgctatca
     1261 atcctggtaa ttcaggtgga gcacttatca atattgaagg acaggttatt ggaattaatt
     1321 ctagtaaaat ttcttctaca tcaaatcaaa cctcaggaca atcgtcagga aatagcgttg
     1381 aaggtatggg atttgccatt ccttcaaatg atgttgttaa gattatcaat caacttgaga
     1441 gtaacggaca agtagagaga cctgctctag gtatttctat ggctggatta agtaatttac
     1501 catccgatgt tattagtaaa ctgaaaatcc caagtaatgt tactaatggt attgtagtag
     1561 catctatcca atctggcatg ccagctcaag gcaaactaaa gaaatacgat gtcattacta
     1621 aagttgacga taaagaagta gcatctccaa gtgatttaca aagtttactc tatggccacc
     1681 aggtagggga ttccataaca gtaacctttt atcgtggtga aaataaacaa acagtaacta
     1741 taaaacttac taaaactagt aaagatttag ctaaacaacg agcaaataac taagtcgact
     1801 ctagaggatc cccgggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac
     1861 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     1921 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     1981 ggcgaatgcc gggaattatt caccactcca agaattggag ccaatcaatt cttgcggaga
     2041 actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc
     2101 agccgcacgc ggcgcatctc ggctgttttg gcggatgaga gaagattttc agcctgatac
     2161 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
     2221 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
     2281 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
     2341 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     2401 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     2461 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
     2521 ggcctttttg cgtttctaca aactcttcct gtcgtcatat ctacaagcca tccccccaca
     2581 gatacggtaa actagcctcg tttttgcatc aggaaagcag ctgttttggc ggatgagaga
     2641 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt
     2701 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
     2761 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat
     2821 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     2881 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa
     2941 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag
     3001 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttcctgt cgtcatatct
     3061 acaagccatc cccccacaga tacggtaaac tagcctcgtt tttgcatcag gaaagcagtc
     3121 gggcagcgtt gggtcctggc gattctgaga cctctgagag gctctcagag ctatctaaag
     3181 ctgagggata tataaatacc ttagaaaatt attcgaagag cttagaagcg aaaatagagc
     3241 gtttagagcg cgaggggctg aaattagaaa aactaaaaac acaaatagct gacctaaaaa
     3301 tcatgtctga gaaagaacta gcggctatta cccctaaaaa aggcgtgttc ggtaaagaat
     3361 atgtggaatt gactaaagag cagtttgaag aatttaaagg gctgatatac cgaagcagaa
     3421 accttgttca tcaaaaagag ctagagaatg agcaattaag gcggatagtg cctctgagac
     3481 gctctaaacg gtttgaagcg agttggaacg agctaaagaa aaaagtaagg gagagagcat
     3541 agagcgtctt aggaacgaaa atagagcgct tagaagtgaa aactcagttt tgagacagca
     3601 aaatgacaaa atgctaggaa aactgaaaga gtttatgcca gataaagcct taaaaaattt
     3661 tatatcagag ttaagagcta ttcagccaat cgtaagggta gttaaacgag tgattgaaaa
     3721 agggctaggc ctttgagcga tttatgccgt gaaagctaat tgacaataag caagggcaaa
     3781 gtacgctagg acgtgacgag ccgaaaggct ttagcgtttc gagccgacac ggacaaagga
     3841 cgtccgccct tggttacttg ttgtcaatta gaccatggaa taaagtaagc ggacatggta
     3901 taatagctag gtcgcaacgt tctttcgcta agttacgaac ttagattgga ggtgagcgct
     3961 gtgaagactt tcctagaact tgttttgata ccttttgtgg ttggcgttgc tgcagaggta
     4021 agtgctgatt ggttgattcg gtatgttcga aacaaacgcg acaaaaatta acctgagttc
     4081 tttttgagga caaaaaagaa agacagtagt tccagctact gttttttttg cgttgtgcta
     4141 ttcgtttcct agaacttcta gcgttaaaat tattatacca cgtttggatt tagaaagtca
     4201 aaatttgagg ttttaggggt gaatttttcg tgaaacgaaa aagagggctg aaaagccctt
     4261 aaaaacccaa ttgcgtagca agggtttttt cttatcttga tacatataga aataatgagt
     4321 ttttttattt tcttgtttta aagcacctca aacccttgat attgctgggt ttttaggtaa
     4381 caaaaaagcc cttgcaattt aataaaataa agtttataat ttaagtgtcc aatttaaaat
     4441 aataaactta ggagaattgc aggaactttt ttatatactc aaaaaaattt ttttgcaaga
     4501 aaattataac atgacaggta ctgaaaatca agtctttaag gactattcta agaatggcaa
     4561 agatagaaag tggcgagaac gcaagttaaa aaatattgag cttgctggtc gtttagagcg
     4621 tttaggatat cgttcgtttg aacgggtcta tcaatgtgcc gaagtgttga agtttatcga
     4681 acaacaagac ggcacgaaaa aactctatca gtcttatttt tgcaaaaata agctctgccc
     4741 aatttgtaac tggagacggt caatgaagta tgcttatcaa gctgaattag tggtaaatga
     4801 agcaatgaaa cgctatccaa aaggtcgctt tctctttttg accttgacga ttaagaacat
     4861 tagtggtgaa aaattaaata aatcaatttc agaaataggg cgagctttta atcgtcttat
     4921 gaaatacaag aaagtcgata aaaatgttat tggctatttg cgagccactg aggtaactta
     4981 ttcaactgag catgagaatt atcaccctca tttgcatgta ttgttatttg tgaaatctag
     5041 ctattttact ggaaataata caaattacat tagccaagaa gaatggacga aactatgggc
     5101 taaggcgatg aaattggatt atacacctgt agttgatatt cgaaccgtca aagctcataa
     5161 acgtaaaaac ttgaagtcag ctattatcga aacagctaaa tatcctgtta agccttttga
     5221 tgtagataca gaagatgtga cattattctc tgaaatggtc aaagaacgga taacagaaga
     5281 tttaacgaat ggtttgcacc gaaaaaggca gattggtttt ggaaagttgt tcaagaaaat
     5341 caaggcggag ttagctcttg atgatgtcga agaagggaat cttgttcaga ccggagcaga
     5401 agaatctgca gaaagtactg gtcgtgaaat tgttgccttt tggaattggg atagaaagaa
     5461 ttattttgtg aggtagctga tgagtaaaac agtatcagaa ttagctcaag aattgggagt
     5521 tagtaggcag tatcttaatc ggattttatc gcaaaataat ctcggtcgaa aaaaagggaa
     5581 taaaaaagta gtttccgata tggacgagaa agttatgcaa gggtttattg ttttctaaaa
     5641 tctgattacc aattagaatg aatatttccc aaatattaaa taataaaaca aaaaaattga
     5701 aaaaagtgtt tccaccattt tttcaatttt tttataattt ttttaatctg ttatttaaat
     5761 agtttatagt taaatttaca ttttcattag tccattcaat attctctcca agataactac
     5821 gaactgctaa caaaattctc tccctatgtt ctaatggaga agattcagcc actgcatttc
     5881 ccgcaatatc ttttggtatg attttacccg tgtccatagt taaaatcata cggcataaag
     5941 ttaatataga gttggtttca tcatcctgat aattatctat taattcctct gacgaatcca
     6001 taatggctct tctcacatca gaaaatggaa tatcaggtag taattcctct aagtcataat
     6061 ttccgtatat tcttttattt tttcgttttg cttggtaaag cattatggtt aaatctgaat
     6121 ttaattcctt ctgaggaatg tatccttgtt cataaagctc ttgtaaccat tctccataaa
     6181 taaattcttg tttgggagga tgattccacg gtaccatttc ttgctgaata ataattgtta
     6241 attcaatata tcgtaagttg cttttatctc ctattttttt tgaaataggt ctaatttttt
     6301 gtataagtat ttctttactt tgatctgtca atggttcaga tacgacgact aaaaagtcaa
     6361 gatcactatt tggttttagt ccactctcaa ctcctgatcc aaacatgtaa gtaccaataa
     6421 ggttattttt taaatgtttc cgaagtattt ttttcacttt attaatttgt tcgtatgtat
     6481 tcaaatatat cctcctcact attttgatta gtacctattt tatatccata gttgttaatt
     6541 aaataaactt aatttagttt atttatagat ttcattggct tctaaatttt ttatctagat
     6601 aataattatt ttagttaatt ttattctaga ttatatatga tatgatcttt catttccata
     6661 aaactaaagt aagtgtaaac ctattcattg ttttaaaaat atctcttgcc agtcacgtta
     6721 cgttattagt tatagttatt ataacatgta ttcacgaacg aaaatcgatc ttctatataa
     6781 aagatatatt atcttatcag tattgtcaat atattcaagg caatctgcct cctcatcctc
     6841 ttcatcctct tcgtcttggt agctttttaa atatggcgct tcatagagta attctgtaaa
     6901 ggtccaattc tcgttttcat acctcggtat aatcttacct atcacctcaa atggttcgct
     6961 gggtttatcg gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7021 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7081 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7141 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     7201 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7261 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7321 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7381 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7441 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7501 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7561 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7621 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7681 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7741 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7801 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7861 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7921 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7981 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     8041 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     8101 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     8161 tggccgattc attaatgcag
//
