LOCUS       Exported                6975 bp DNA     circular SYN 21-JUL-2025
DEFINITION  Shuttle vector pDL276, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Dunny GM, Lee LN, LeBlanc DJ.
  TITLE     Improved electroporation and cloning vector system for gram-positive
            bacteria
  JOURNAL   Appl. Environ. Microbiol. 57 (4), 1194-1201 (1991)
  PUBMED    1905518
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Dunny GM, Bae T.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-1999) Microbiology, University of Minnesota, Box 
            196 UMHC, 1460 Mayo Building, 420 Delaware St. SE, Minneapolis, MN 
            55455, USA
REFERENCE   3  (bases 1 to 6975)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6975)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6975)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "1991"; volume: "57"; issue: "4"; pages:
            "1194-1201"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-DEC-1999) Microbiology, University of Minnesota, Box 196 UMHC,
            1460 Mayo Building, 420 Delaware St. SE, Minneapolis, MN 55455, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(237..825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1746..2537
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      5397..5424
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      5556..5599
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(5895..5922)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(6014..6100)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     6574..6590
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6591..6647
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(6660..6676)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6684..6700)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6708..6738)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6753..6774)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      661 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1201 agaagtggtc cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt
     1261 atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct
     1321 accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata
     1381 ctgataagat aatatatctt ttatatagaa gatatcgccg tatgtaagga tttcaggggg
     1441 caaggcatag gcagcgcgct tatcaatata tctatagaat gggcaaagca taaaaacttg
     1501 catggactaa tgcttgaaac ccaggacaat aaccttatag cttgtaaatt ctatcataat
     1561 tgtggtttca aaatcggctc cgtcgatact atgttatacg ccaactttca aaacaacttt
     1621 gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc
     1681 gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat
     1741 aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct
     1801 gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg
     1861 aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac
     1921 gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact
     1981 ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct
     2041 cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca
     2101 tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc
     2161 gcttagccga attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact
     2221 gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa
     2281 agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga
     2341 aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt
     2401 atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg
     2461 agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt
     2521 tactggatga attgttttag tacctagatt tagatgtcta aaaagcttta actacaagct
     2581 ttgacatcta atcttttctg aagtacatcc gcaactgtcc atactctgat gttttatatc
     2641 ttttctaaaa gttcgctaga taggggtccc gagcgcctac gaggaatttg tatcggtcct
     2701 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     2761 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtacttt
     2821 ctcgtccata tcggaaacta cttttttatt cccttttttt cgaccgagat tattttgcga
     2881 taaaatccga ttaagatact gcctactaac tcccaattct tgagctaatt ctgatactgt
     2941 tttactcatc agctacctca caaaataatt ctttctatcc caattccaaa aggcaacaat
     3001 ttcacgacca gtactttctg cagattcttc tgctccggtc tgaacaagat tcccttcttc
     3061 gacatcatca agagctaact ccgccttgat tttcttgaac aactttccaa aaccaatctg
     3121 cctttttcgg tgcaaaccat tcgttaaatc ttctgttatc cgttctttga ccatttcaga
     3181 gaataatgtc acatcttctg tatctacatc aaaaggctta acaggatatt tagctgtttc
     3241 gataatagct gacttcaagt ttttacgttt atgagctttg acggttcgaa tatcaactac
     3301 aggtgtataa tccaatttca tcgccttagc ccatagtttc gtccattctt cttggctaat
     3361 gtaatttgta ttatttccag taaaatagct agatttcaca aataacaata catgcaaatg
     3421 agggtgataa ttctcatgct cagttgaata agttacctca gtggctcgca aatagccaat
     3481 aacattttta tcgactttct tgtatttcat aagacgatta aaagctcgcc ctatttctga
     3541 aattgattta tttaattttt caccactaat gttcttaatc gtcaaggtca aaaagagaaa
     3601 gcgacctttt ggatagcgtt tcattgcttc atttaccact aattcagctt gataagcata
     3661 cttcattgac cgtctccagt tacaaattgg gcagagctta tttttgcaaa aataagactg
     3721 atagagtttt ttcgtgccgt cttgttgttc gataaacttc aacacttcgg cacattgata
     3781 gacccgttca aacgaacgat atcctaaacg ctctaaacga ccagcaagct caatattttt
     3841 taacttgcgt tctcgccact ttctatcttt gccattctta gaatagtcct taaagacttg
     3901 attttcagta cctgtcatgt tataattttc ttgcaaaaaa atttttttga gtatataaaa
     3961 aagttcctgc aattctccta agtttattat tttaaattgg acacttaaat tataaacttt
     4021 attttattaa attgcaaggg cttttttgtt acctaaaaac ccagcaatat caagggtttg
     4081 aggtgcttta aaacaagaaa ataaaaaaac tcattatttc tatatgtatc aagataagaa
     4141 aaaacccttg ctacgcatgg gtttttaagg gcttttcagc cctctttttc gtttcacgaa
     4201 aaattcaccc ctaaaacctc aaattttgac tttctaaatc caaacgtggt ataataattt
     4261 taacgctaga agttctagga aacgaatagc acaacgcaaa aaaaacagta gctggaacta
     4321 ctgtctttct tttttgtcct caaaaagaac tcaggttaat ttttgtcgcg tttgtttcga
     4381 acataccgaa tcaaccaatc agcacttacc tctgcagcaa cgccaaccac aaaaggtatc
     4441 aaaacaagtt ctaggaaagt cttcacagcg ctcacctcca atctaagttc gtaacttagc
     4501 gaaagaacgt tgcgacctag ctattatacc atgtccgctt actttattcc atggtctaat
     4561 tgacaacaag taaccaaggg cggacgtcct ttgtccgtgt cggctcgaaa cgctaaagcc
     4621 tttcggctcg tcacgtccta gcgtactttg cccttgctta ttgtcaatta gctttcacgg
     4681 cataaatcgc tcaaaggcct agcccttttt caatcactcg tttaactacc cttacgattg
     4741 gctgaatagc tcttaactct gatataaaat tttttaaggc tttatctggc ataaactctt
     4801 tcagttttcc tagcattttg tcattttgct gtctcaaaac tgagttttca cttctaagcg
     4861 ctctattttc gttcctaaga cgctctatgc tctctccctt acttttttct ttagctcgtt
     4921 ccaaactcgc ttcaaaaccg tttttagagc gtctcagagg cactatccgc cttaattgct
     4981 cattctctag ctctttttga tgaacaaggt ttctgcttcg gtatatcagc cctttaaatt
     5041 cttcaaactg ctctttagtc aattccacat attctttacc gaacacgcct tttttagggg
     5101 taatagccgc tagttctttc tcagacatga tttttaggtc agctatttgt gtttttagtt
     5161 tttctaattt cagcccctcg cgctctaaac gctctatttt cgcttctaag ctcttcgaat
     5221 aattttctaa ggtatttata tatccctcag ctttagatag ctctgagagc ctctcagagg
     5281 tctcagaatc ccaacgctgc ccgactgctt tcctgatgca aaaacgaggc tagtttaccg
     5341 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aacgcaaaaa
     5401 ggccatccgt caggatggcc ttctgcttaa tttgatgcct ggcagtttat ggcgggcgtc
     5461 ctgcccgcca ccctccgggc cgttgcttcg caacgttcaa atccgctccc ggcggatttg
     5521 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg
     5581 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcatg gggagacccc
     5641 acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc aggtgggacc
     5701 accgcgctac tgccgccagg caaattctgt tttatcagac cgcttctgcg ttctgattta
     5761 atctgtatca ggctgaaaat cttctctcat ccgccaaaac agctgctttc ctgatgcaaa
     5821 aacgaggcta gtttaccgta tctgtggggg gatggcttgt agatatgacg acaggaagag
     5881 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg
     5941 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcgca acgttcaaat
     6001 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     6061 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggca gttccctact
     6121 ctcgcatggg gagaccccac actaccatcg gcgctacggc gtttcacttc tgagttcggc
     6181 atggggtcag gtgggaccac cgcgctactg ccgccaggca aattctgttt tatcagaccg
     6241 cttctgcgtt ctgatttaat ctgtatcagg ctgaaaatct tctctcatcc gccaaaacag
     6301 ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag
     6361 ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg
     6421 tgaataattc ccggcattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     6481 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     6541 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc
     6601 tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg
     6661 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6721 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6781 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6841 ggccaacgcg cggggagagg cggtttgcgt attggagctt gttgtaactg aaaaaggaaa
     6901 attattgtgc caggcagttg aaagtcagca ccttttaacg agtgctgaaa tgacggctaa
     6961 atgggaaacg tattt
//
