LOCUS       40924_14725        4311 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pDIVA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Laufer M, Mohammad H, Varrelmann M, Maiss E.
  TITLE     Construction of infectious full-length cDNA clones of Beet 
            soil-borne mosaic virus and Beet necrotic yellow vein virus A-type 
            by isothermal in vitro recombination analysis of systemic movement, 
            vector transmissions and transreplication properties
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Laufer M, Mohammad H, Varrelmann M, Maiss E.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2016) Phytomedicine, Leibniz Universitaet Hannover
            - Institute of Horticultural Production Systems, Herrenhaeuser 
            Strasse 2, Hannover 30419, Germany
REFERENCE   3  (bases 1 to 4311)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4311)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-AUG-2016) Phytomedicine, Leibniz Universitaet Hannover - 
            Institute of Horticultural Production Systems, Herrenhaeuser Strasse
            2, Hannover 30419, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method :: CLC Main Workbench version 6.8.1 Sequencing 
            Technology      :: Sanger dideoxy sequencing ##Assembly-Data-END## 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..622
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     CDS             839..1630
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             1932..3077
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    complement(3235..3259)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        3412..3754
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    3755..3839
                     /label=HDVagrz
                     /note="HDVagrz"
     misc_feature    3840..3874
                     /label=35 bp spacer
                     /note="35 bp spacer"
     misc_feature    3875..4079
                     /label=pA35S
                     /note="pA35S"
     primer_bind     complement(4109..4125)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(4206..4230)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      4310..4311
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg
       61 cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc
      121 tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact tgaggggccg
      181 actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg gcgacgtgga
      241 gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc ccacagatga
      301 tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc gcgactactg
      361 acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga tgaggggcgc
      421 acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc aagggtttcc
      481 gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca atatttataa
      541 accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg
      601 cccccccttc tcgaaccctc ccggcccgct cgagttgcag catcacccat aattgtggtt
      661 tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag
      721 ctgttttctg gtatttaaag ttttagaatg caaggaacag tgaattggag ttcgtcttgt
      781 tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga aataataaat
      841 ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa
      901 agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct
      961 atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa
     1021 ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc accttgaacg
     1081 gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga
     1141 gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct
     1201 ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc
     1261 cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga
     1321 agacacccca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga
     1381 agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg
     1441 caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat
     1501 tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg tcgagctatt
     1561 ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga
     1621 tgaattgttt tagtacctag atgtggcgca acgatgccgg cgacaagcag gagcgcaccg
     1681 acttcttccg catcaagtgt tttggctctc aggccgaggc ccacggcaag tatttgggca
     1741 aggggtcgct ggtattcgtg cagggcaaga ttcggaatac caagtacgag aaggacggcc
     1801 agacggtcta cgggaccgac ttcattgccg ataaggtgga ttatctggac accaaggcac
     1861 caggcgggtc aaatcaggaa taagggcaca ttgccccggc gtgagtcggg gcaatcccgc
     1921 aaggagggtg aatgaatcgg acgtttgacc ggaaggcata caggcaagaa ctgatcgacg
     1981 cggggttttc cgccgaggat gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc
     2041 gcgaaacctt ccagtccgtc ggctcgatgg cccagcaagc tacggccaag atcgagcgcg
     2101 acagcgtgca actggctccc cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc
     2161 gtcgtctcga acaggaggcg gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa
     2221 ctatgacgac caagaagcga aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg
     2281 ccaagcaggc cgcgttgctg aaacacacga agcagcagat caaggaaatg cagctttcct
     2341 tgttcgatat tgcgccgtgg ccggacacga tgcgagcgat gccaaacgac acggcccgct
     2401 ctgccctgtt caccacgcgc aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca
     2461 ttttccacgt caacaaggac gtgaagatca cctacaccgg cgtcgagctg cgggccgacg
     2521 atgacgaact ggtgtggcag caggtgttgg agtacgcgaa gcgcacccct atcggcgagc
     2581 cgatcacctt cacgttctac gagctttgcc aggacctggg ctggtcgatc aatggccggt
     2641 attacacgaa ggccgaggaa tgcctgtcgc gcctacaggc gacggcgatg ggcttcacgt
     2701 ccgaccgcgt tgggcacctg gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc
     2761 gtggcaagaa aacgtcccgt tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg
     2821 ctggcgacca ctacacgaaa ttcatatggg agaagtaccg caagctgtcg ccgacggccc
     2881 gacggatgtt cgactatttc agctcgcacc gggagccgta cccgctcaag ctggaaacct
     2941 tccgcctcat gtgcggatcg gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg
     3001 aagcctgcga agagttgcga ggcagcggcc tggtggaaca cgcctgggtc aatgatgacc
     3061 tggtgcattg caaacgctag ggccttgtgg ggtcagttcc ggctgggggt tcagcagcca
     3121 gcgctttact gagatctcct gtggttggca tgcacataca aatggacgaa cggataaacc
     3181 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
     3241 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
     3301 agctccaccg cggtggcggc cgctctagag atccgtcaac atggtggagc acgacactct
     3361 cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt
     3421 tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt
     3481 catcaaaagg acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg
     3541 aaaggctatc gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
     3601 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
     3661 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
     3721 ctctatataa ggaagttcat ttcatttgga gagggggtcg gcatggcatc tccacctcct
     3781 cgcggtccga cctgggcatc cgaaggagga cgcacgtcca ctcggatggc taagggagaa
     3841 gctggcccga gctcgatcga gaagtactag agtccgcaaa aatcaccagt ctctctctac
     3901 aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa
     3961 ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat
     4021 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg
     4081 acctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta
     4141 ccaaaaccac cccagtactt taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
     4201 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
     4261 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccactagag a
//
