LOCUS       62056_8435       12953 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12953)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        188..206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(955..971)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             986..1450
                     /codon_start=1
                     /label=VN155(I152L)
                     /note="improved N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     promoter        3882..3900
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3945..4295
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             4300..5322
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5869..5887)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(5956..5987)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6002..6023)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    6391..6415
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7715..8341
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8778..9842
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     misc_feature    10449..10589
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10775..11363)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11453..12244)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12669..12693
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(12771..12945)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 aacattaacg tttacaattt cgcgccattc gccattcagg ctgcgcaact gttgggaagg
       61 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
      121 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      181 tgaattgtaa tacgactcac tatagggcga attgggtacc gcatgcggag agacggacgg
      241 acgcagagag aagggctgag taataagcgc cactgcgcca gacagctctg gcggctctga
      301 ggtgcagtgg atgattatta atccgggacc ggccgcccct ccgccccgaa gtggaaaggc
      361 tggtgtgccc ctcgttgacc aagaatctat tgcatcatcg gagaatatgg agcttcatcg
      421 aatcaccggc agtaagcgaa ggagaatgtg aagccagggg tgtatagccg tcggcgaaat
      481 agcatgccat taacctaggt acagaagtcc aattgcttcc gatctggtaa aagattcacg
      541 agatagtacc ttctccgaag taggtagagc gagtacccgg cgcgtaagct ccctaattgg
      601 cccatccggc atctgtaggg cgtccaaata tcgtgcctct cctgctttgc ccggtgtatg
      661 aaaccggaaa ggccgctcag gagctggcca gcggcgcaga ccgggaacac aagctggcag
      721 tcgacccatc cggtgctctg cactcgacct gctgaggtcc ctcagtccct ggtaggcagc
      781 tttgccccgt ctgtccgccc ggtgtgtcgg cggggttgac aaggtcgttg cgtcagtcca
      841 acatttgttg ccatattttc ctgctctccc caccagctgc tcttttcttt tctctttctt
      901 ttcccatctt cagtatattc atcttcccat ccaagaacct ttaatcaagc ttatcgatac
      961 cgtcgacctc gactctagag ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     1021 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1081 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1141 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     1201 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1261 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1321 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1381 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1441 tatcatggcc gaccgccccg cctgcaagat ccccaacgac ctcaagcaga aggtcatgaa
     1501 ccaccacgtg atgtatcccc atcatggtcc cccgatgcct cctccgcaga agccggagac
     1561 tttcatgctg tccagcgagg cgcagcagag tctgcctcac gatgcgcaag tcgctctgca
     1621 gcaagtcgat aacctgaaat acttccttct gtcggctcct gtggactggc aacccgatca
     1681 attcgttcga cgcttcttgc tgcccacggg cgaacacatt tcctgcgtgc tttggagcaa
     1741 cctcttccac atctcaggaa ctgatatcgt cagatgtctg gcgttccgtt tcacagcatt
     1801 tggccgcccc gtcaagaact cgaaaaagtt cgaagaaggc atcttttccg acttgcgaaa
     1861 cctcaaggcc ggtaccgatg ctacgcttga agagcccaag agccccttcc tcgacttcct
     1921 ctacaagaac aactgtatcc gcacccagaa gaagcaaaag gtcttctact ggtatagcgt
     1981 cccgcacgat cgtctcttcc tcgatgccct tgaacgggac ttgaagaggg agaagatggg
     2041 acaggaggcc accaccgttg ccgtcagcga gcccgccttg tctttcgagt tcgattcgtc
     2101 ccagtcgctc tatgagcagc tcaccaaggc tcagcaagca aatacttctt cgtttgctgc
     2161 acacgcaagt acgacatttg gccagcccac ctccccaatc gtgcggtccg ttgacgctat
     2221 gcctcctcct cagatggctc cccagatggc tcccccggcg ctgcctttcc taccggacga
     2281 ttcggccaac acggcgatct acaatgcgat ccccttgccc cctacgctag cccagaattt
     2341 catcaagcgt gagcaggatt tcactcccat ccagtacgac cgcaacggga tgcccatcgc
     2401 ccgcatgcac cagcgtcacg cctccatgcc gacctttgtc gagtactcgc cggccccctc
     2461 cttcgtttcc tcccattttg aagactacag caaccgcggc ctgtcgtttg agcccgtgac
     2521 tcccccgcag catcatgtgc ccttgggtcc cgagcccgcg tacatcgcca atgaagacac
     2581 gggcctctac acggccatcc cggacatttc gtccgccccg tctttcaacc ccttgatgca
     2641 actgcccccc tcaaacctgg ccagcgccca tttcccggcc ccaccgcgga cgttccccta
     2701 ctcggtcatt gagggttctc ccacctataa gcagcggaga cgccgctctt cggtcacccc
     2761 gggagccgcc aacgctgcca tgaccacccc ccaaaccaca gccgctcccg cccagacaat
     2821 ggcgtacgca gcacacaaac ccagtgacct gcggagatcc atgtcgagct cggtggctcc
     2881 cgctgtgacc gagggcggca atgaatcgcg aacccagcgc gccacggcga gctacccagc
     2941 ggttcttccg cagaaggact tgttacagga aatttcccgt catggtaccc cgctctccaa
     3001 ccttgaggaa accaccagcc agaatgctct gtccctgaac aacgtccggg atgacctggc
     3061 ggctttcccg accgatgaga cggttgatgc cgctgtgcag aacagtgcaa ccaataaggc
     3121 cgagcgatat gttccgggcc cggtccgccg tgcccgaagc gctaccatga tggagctggg
     3181 gccctatccg caaaaatccc actcgtgtcc aatcccatcg tgtggacgac ttttcaagag
     3241 attagagcac ttgaagcgac atgttcgaac ccacacccaa gagcgaccat acccttgccc
     3301 ttactgcaac aaggcgttct cgcgatcgga caaccttgca cagcaccgtc ggatccacga
     3361 ggctcaacta gatggtcagc cgccgctgtc aaccaatgag gaagaattgg agaacgagga
     3421 aaatgaattc ggctcgccgg acgaggactt gtcaccgcaa gacacttcaa ttccgacctc
     3481 catgatgaac atgaccccta tgacctccat gccgactacc atggccacca caatgagcat
     3541 gccacctatg catactatgg ttgctccgaa tatgattgcg cctcagctct tgcagcagca
     3601 gatatgactc gaggccccgt ccggtcctgc ccgtcaccga gatccactta acgttactga
     3661 aatcatcaaa cagcttgacg aatctggata taagatcgtt ggtgtcgatg tcagctccgg
     3721 agttgagaca aatggtgttc aggatctcga taagatacgt tcatttgtcc aagcagcaaa
     3781 gagtgccttc tagtgattta atagctccat gtcaacaaga ataaaacgcg tttcgggttt
     3841 acctcttcca gatacagctc atctgcaatg cggatccgcg caattaaccc tcactaaagg
     3901 gaacaaaagc tggagctcca ccgcggtggc ggccgcgacg ttaactgata ttgaaggagc
     3961 atttttgggc ttggctggag ctagtggagg tcaacacatc aatgctattt tggtttagtc
     4021 gtccaggcgg atcacaaaat ttgtgtcgtt tgacaagatg gttcatttag gcaactggtc
     4081 agatcagccc acttgtaagc agtagcggcg gcgctcgaag tgtgactctt attagcagac
     4141 aggaacgagg acattattat catctgctgc ttggtgcacg ataacttgtg cgtttgtcaa
     4201 gcaaggtaag tgaacgaccc ggtcatacct tcttaagttc gcccttcctc cctttatttc
     4261 agattcaatc tgacttacct attctaccca agcatcgata tgaaaaagcc tgaactcacc
     4321 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     4381 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     4441 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     4501 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
     4561 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     4621 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     4681 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     4741 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     4801 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     4861 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     4921 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     4981 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     5041 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     5101 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     5161 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     5221 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     5281 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagagtag atgccgaccg
     5341 gatcgatcca cttaacgtta ctgaaatcat caaacagctt gacgaatctg gatataagat
     5401 cgttggtgtc gatgtcagct ccggagttga gacaaatggt gttcaggatc tcgataagat
     5461 acgttcattt gtccaagcag caaagagtgc cttctagtga tttaatagct ccatgtcaac
     5521 aagaataaaa cgcgttttcg ggtttacctc ttccagatac agctcatctg caatgcatta
     5581 atgcattgac tgcaacctag taacgccttc aggctccggc gaagagaaga atagcttagc
     5641 agagctattt tcattttcgg gagacgagat caagcagatc aacggtcgtc aagagaccta
     5701 cgagactgag gaatccgctc ttggctccac gcgactatat atttgtctct aattgtactt
     5761 tgacatgctc ctcttcttta ctctgatagc ttgactatga aaattccgtc accagccctg
     5821 actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg
     5881 gttaattccg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5941 gctcacaatt ccacacaaca tacgagccgg aaghcataaa gtgtaaagcc tggggtgcct
     6001 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6061 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6121 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6181 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6241 caggaaagaa catgaaggct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6301 acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt
     6361 ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     6421 agaaaagagc gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt
     6481 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
     6541 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
     6601 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
     6661 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
     6721 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
     6781 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
     6841 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
     6901 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
     6961 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
     7021 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
     7081 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
     7141 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
     7201 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
     7261 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
     7321 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
     7381 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
     7441 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
     7501 agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
     7561 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
     7621 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
     7681 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
     7741 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
     7801 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
     7861 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
     7921 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
     7981 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
     8041 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
     8101 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
     8161 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
     8221 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
     8281 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
     8341 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
     8401 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
     8461 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
     8521 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
     8581 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
     8641 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
     8701 gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc
     8761 cgaggaatcg gcgtgacggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     8821 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
     8881 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     8941 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     9001 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     9061 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     9121 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
     9181 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     9241 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     9301 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     9361 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     9421 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     9481 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
     9541 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     9601 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     9661 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     9721 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     9781 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
     9841 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
     9901 gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
     9961 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
    10021 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
    10081 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
    10141 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
    10201 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
    10261 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
    10321 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
    10381 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
    10441 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
    10501 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    10561 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
    10621 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    10681 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    10741 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    10801 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    10861 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    10921 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    10981 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    11041 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    11101 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    11161 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
    11221 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    11281 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    11341 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    11401 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt
    11461 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
    11521 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
    11581 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
    11641 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
    11701 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
    11761 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
    11821 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
    11881 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
    11941 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
    12001 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
    12061 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
    12121 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
    12181 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
    12241 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
    12301 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
    12361 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
    12421 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
    12481 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
    12541 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
    12601 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
    12661 ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac
    12721 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt
    12781 ttagtactgg attttggttt taggaattag aaattttatt gatagaagta ttttacaaat
    12841 acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc
    12901 ctaattccct tatctgggaa ctactcacac attattatgg agaaacttgg caa
//
