LOCUS       62056_8445       11518 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        191..209
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        654..837
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             861..1649
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      2042..2294
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2913..3626)
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        complement(4434..4452)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(4521..4552)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4567..4588)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    4956..4980
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6280..6906
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7343..8407
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8476..8670
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9014..9154
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9340..9928)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10018..10809)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11234..11258
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(11336..11510)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 aacattaacg tttacaattt cgcgccattc gccattcagg ctgcgcaact gttgggaagg
       61 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
      121 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      181 tgagcgcgcg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt
      241 cgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatccg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctc caccgcggtg gcggccgcga cgttaactga
      361 tattgaagga gcactttttg ggcttggctg gagctagtgg aggtcaacaa tgaatgccta
      421 ttttggttta gtcgtccagg cggtgagcac aaaatttgtg tcgtttgaca agatggttca
      481 tttaggcaac tggtcagatc agccccactt gtagcagtag cggcggcgct cgaagtgtga
      541 ctcttattag cagacaggaa ctaggacatt atcatcatct gctgcttggt gcacgataac
      601 ttggtgcgtt tgtcaagcaa ggtaagtgaa cgacccggtc ataccttctt aaggggtttc
      661 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
      721 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
      781 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
      841 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
      901 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
      961 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     1021 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     1081 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     1141 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     1201 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     1261 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     1321 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     1381 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     1441 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     1501 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     1561 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     1621 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     1681 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     1741 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     1801 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     1861 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     1921 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     1981 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     2041 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     2101 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     2161 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     2221 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     2281 tatgttacta gatcacgcgt tttcgggttt acctcttcca gatacagctc aactgcaatg
     2341 cattaatgca ttggacctcg caaccctagt acgcccttca ggctccggcg aagcagaaga
     2401 atagcttagc agagtctatt ttcattttcg ggagacgaga tcaagcagat caacggtcgt
     2461 caagagacct acgagactga ggaatccgct cttggctcca cgcgactata tatttgtctc
     2521 taattgtact ttgacatgct cctcttcttt actctgatag cttgactatg aaaattccgt
     2581 caccagccct gactagtgca ttgcagatga gctgtatctg gaagaggtaa acccgaaacg
     2641 cgttttattc ttgttgacat ggagctatta aatcactaga aggcactctt tgctgcttgg
     2701 acaaatgaac gtatcttatc gagatcctga acaccatttg tctcaactcc ggagctgaca
     2761 tcgacaccaa cgatcttata tccagattcg tcaagctgtt tgatgatttc agtaacgtta
     2821 agtggatctc ggtgacgggc aggaccggac ggggcggtac cgggcccccc ctcgaggtcg
     2881 acggtatcga taagcttgat atcgaattct tacttgtata gttcatccat gccatgtgta
     2941 atcccagcag ctgttacaaa ctcaagaagg accatgtggt ctctcttttc gttgggatct
     3001 ttcgaaaggg cagattgtgt ggacaggtaa tggttgtctg gtaaaaggac agggccatcg
     3061 ccaattggag tattttgttg ataatggtct gctagttgaa cgcttccatc ttcaatgttg
     3121 tgtctaattt tgaagttaac tttgattcca ttcttttgtt tgtctgccat gatgtataca
     3181 ttgtgtgagt tatagttgta ttccaatttg tgtccaagaa tgtttccatc ttctttaaaa
     3241 tcaatacctt ttaactcgat tctattaaca agggtatcac cttcaaactt gacttcagca
     3301 cgtgtcttgt agttcccgtc atctttgaaa aatatagttc tttcctgtac ataaccttcg
     3361 ggcatggcac tcttgaaaaa gtcatgctgt ttcatatgat ctgggtatct cgcaaagcat
     3421 tgaacaccat aaccgaaagt agtgacaagt gttggccatg gaacaggtag ttttccagta
     3481 gtgcaaataa atttaagggt aagttttccg tatgttgcat caccttcacc ctctccactg
     3541 acagaaaatt tgtgcccatt aacatcacca tctaattcaa caagaattgg gacaactcca
     3601 gtgaaaagtt cttctccttt actcatggat cctctagagt cgaggtcgac ggtatcgata
     3661 agcttgatta aaggttcttg gatgggaaga tgaatatact gaagatggga aaagaaagag
     3721 aaaagaaaag agcagctggt ggggagagca ggaaaatatg gcaacaaatg ttggactgac
     3781 gcaacgacct tgtcaacccc gccgacacac cgggcggaca gacggggcaa agctgcctac
     3841 cagggactga gggacctcag caggtcgagt gcagagcacc ggatgggtcg actgccagct
     3901 tgtgttcccg gtctgcgccg ctggccagct cctgagcggc ctttccggtt tcatacaccg
     3961 ggcaaagcag gagaggcacg atatttggac gccctacaga tgccggatgg gccaattagg
     4021 gagcttacgc gccgggtact cgctctacct acttcggaga aggtactatc tcgtgaatct
     4081 tttaccagat cggaagcaat tggacttctg tacctaggtt aatggcatgc tatttcgccg
     4141 acggctatac acccctggct tcacattctc cttcgcttac tgccggtgat tcgatgaagc
     4201 tccatattct ccgatgatgc aatagattct tggtcaacga ggggcacacc agcctttcca
     4261 cttcggggcg gaggggcggc cggtcccgga ttaataatca tccactgcac ctcagagccg
     4321 ccagagctgt ctggcgcagt ggcgcttatt actcagccct tctctctgcg tccgtccgtc
     4381 tctccgcatg ctctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag
     4441 tgagggttaa ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     4501 tatccgctca caattccaca caacatacga gccggaaghc ataaagtgta aagcctgggg
     4561 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4621 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4681 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4741 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4801 taacgcagga aagaacatga aggctggcgt aatagcgaag aggcccgcac cgatcgccct
     4861 tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt
     4921 gtcgtttccc gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
     4981 ctaagagaaa agagcgttta ttagaataac ggatatttaa aagggcgtga aaaggtttat
     5041 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
     5101 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
     5161 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     5221 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     5281 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     5341 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     5401 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     5461 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     5521 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
     5581 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     5641 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
     5701 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
     5761 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
     5821 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
     5881 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
     5941 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
     6001 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
     6061 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
     6121 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg
     6181 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
     6241 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
     6301 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
     6361 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
     6421 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
     6481 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
     6541 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
     6601 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
     6661 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
     6721 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
     6781 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
     6841 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
     6901 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
     6961 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
     7021 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
     7081 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
     7141 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
     7201 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
     7261 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc
     7321 aagcccgagg aatcggcgtg acggtcgcaa accatccggc ccggtacaaa tcggcgcggc
     7381 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
     7441 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
     7501 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
     7561 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
     7621 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
     7681 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
     7741 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
     7801 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
     7861 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
     7921 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
     7981 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
     8041 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga
     8101 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
     8161 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
     8221 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
     8281 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
     8341 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
     8401 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
     8461 aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
     8521 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
     8581 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
     8641 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct
     8701 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
     8761 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
     8821 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg
     8881 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     8941 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     9001 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     9061 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     9121 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     9181 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     9241 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9301 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9361 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9421 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9481 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9541 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9601 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9661 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9721 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9781 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9841 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9901 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9961 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta ggtactaaaa
    10021 caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa
    10081 gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca
    10141 gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact
    10201 tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag
    10261 ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg
    10321 agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc
    10381 caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga
    10441 gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc
    10501 ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc
    10561 atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat
    10621 catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt
    10681 taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc
    10741 cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat
    10801 tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct
    10861 aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag
    10921 aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
    10981 attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct
    11041 accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata
    11101 gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc
    11161 ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
    11221 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
    11281 ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt cgggggatct
    11341 ggattttagt actggatttt ggttttagga attagaaatt ttattgatag aagtatttta
    11401 caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga aaccctatag
    11461 gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa cttggcaa
//
