LOCUS       40924_14695        6993 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid for transforming fungi to hygromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Cullen D, Leong SA, Wilson LJ, Henner DJ.
  TITLE     Transformation of Aspergillus nidulans with the 
            hygromycin-resistance gene, hph.
  JOURNAL   Gene 1987;57:21-6.
  PUBMED    3322945
REFERENCE   2  (bases 1 to 6993)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6993)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1987"; volume: "57"; pages: "21-6"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     A ClaI site is present just upstream of the HygR start codon.
FEATURES             Location/Qualifiers
     source          1..6993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1540..1728
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    1833..1973
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2159..2747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2921..3778)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3779..3883)
                     /label=AmpR promoter
     promoter        4874..5230
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             5235..6257
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      6429..6987
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus
                     nidulans trpC gene"
ORIGIN
        1 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
       61 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
      121 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
      181 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
      241 ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt
      301 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
      361 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg
      421 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat
      481 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa
      541 gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
      601 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
      661 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
      721 aggatcgctc gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc
      781 gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct
      841 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
      901 aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa
      961 ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc
     1021 gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg
     1081 cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt
     1141 tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct
     1201 gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg
     1261 cggaagtcag cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta
     1321 ccctgtggaa cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt
     1381 ctctggtccc gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg
     1441 gcatgttcat catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt
     1501 acccccatga acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc
     1561 gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac
     1621 gagctggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag
     1681 ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     1741 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     1801 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     1861 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     1921 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     1981 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2041 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2101 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2161 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2221 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2281 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2341 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2401 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2461 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2521 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2581 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2641 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2701 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2761 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2821 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2881 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2941 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3001 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3061 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3121 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3181 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     3241 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3301 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3361 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3421 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3481 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     3541 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3601 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3661 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3721 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3781 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3841 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3901 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     3961 tcacgaggcc ctttcgtctt caagaattca tgccagttgt tccagtgatc ttcgtttcga
     4021 agatggacat ccaatttgtg caagttattc ggcctacctg gctgtggccg aggcagcgtt
     4081 atcatgaccg tcgctgttca aagataaggc gagaagtttg cgggctgtct tgacgatatg
     4141 gcttcgttca gacagatata gttcccggag tcgcaggctg ctattcttct ccgaaacaaa
     4201 ctcggctgca ctgtttccat caccgggtct ggcgtttgag gatgtcagcg aaactcggcc
     4261 gcaagtgaca ccgaaaagta tcgactccgg tgcccgtttc aagctagtgg cttcctcaac
     4321 agcgagtcgg ccaggagacg tgaagcagga cgggttgcca tccagaccgt gaccgaacgc
     4381 gttgatttca tcaatcccag ccttttcgct caaccaaaga gcatcggctt tgatttcctt
     4441 caggtcatac gaggcttgtg caatggtctc cgcatggata tcgcgtgctg ttctcctatc
     4501 aactcggaat tttgtcttag gggatggcgt aggaacctcg aatgctgatc aggatgtgac
     4561 aaaaacgact cgaaaacccg ggttcatcgg tgtgctttcg ggatcgcaag cgtaaagaaa
     4621 gattcactct tccaagacct agaagtatag caaatcagca gcagaccatc aatgtatagc
     4681 gaatgcgccc atacaaaacg tgaactcccc ggagaagcac tttgtccagg gacgggaaat
     4741 aggcttccgg aacgggagca ttggcagcac agctatatca ttctaagtaa acaaatgtaa
     4801 tgagcaagcg gacgagtgct gaaacctcct atgccgtgaa ggccgacgaa agcgcgttgt
     4861 tggattaagt cgacagaaga tgatattgaa ggagcatttt tgggcttggc tggagctagt
     4921 ggaggtcaac acatcaatgc tattttggtt tagtcgtcca ggcggatcac aaaatttgtg
     4981 tcgtttgaca agatggttca tttaggcaac tggtcagatc agcccacttg taagcagtag
     5041 cggcggcgct cgaagtgtga ctcttattag cagacaggaa cgaggacatt attatcatct
     5101 gctgcttggt gcacgataac ttgtgcgttt gtcaagcaag gtaagtgaac gacccggtca
     5161 taccttctta agttcgccct tcctcccttt atttcagatt caatctgact tacctattct
     5221 acccaagcat cgatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     5281 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     5341 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     5401 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     5461 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     5521 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     5581 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     5641 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     5701 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     5761 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     5821 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     5881 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     5941 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     6001 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     6061 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     6121 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     6181 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     6241 gtccgagggc aaaggaatag agtagatgcc gaccggatcc acttaacgtt actgaaatca
     6301 tcaaacagct tgacgaatct ggatataaga tcgttggtgt cgatgtcagc tccggagttg
     6361 agacaaatgg tgttcaggat ctcgataaga tacgttcatt tgtccaagca gcaaagagtg
     6421 ccttctagtg atttaatagc tccatgtcaa caagaataaa acgcgttttc gggtttacct
     6481 cttccagata cagctcatct gcaatgcatt aatgcattga ctgcaaccta gtaacgcctt
     6541 caggctccgg cgaagagaag aatagcttag cagagctatt ttcattttcg ggagacgaga
     6601 tcaagcagat caacggtcgt caagagacct acgagactga ggaatccgct cttggctcca
     6661 cgcgactata tatttgtctc taattgtact ttgacatgct cctcttcttt actctgatag
     6721 cttgactatg aaaattccgt caccagccct gggttcgcaa agataattgc atgtttcttc
     6781 cttgaactct caagcctaca ggacacacat tcatcgtagg tataaacctc gaaatcattc
     6841 ctactaagat ggtatacaat agtaaccatg gttgcctagt gaatgctccg taacacccaa
     6901 tacgccggcc gaaacttttt tacaactctc ctatgagtcg tttacccaga atgcacaggt
     6961 acacttgttt agaggtaatc cttctttcta gag
//
